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P23613

- HYALP_CAVPO

UniProt

P23613 - HYALP_CAVPO

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Protein

Hyaluronidase PH-20

Gene

SPAM1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei147 – 1471Proton donorBy similarity

GO - Molecular functioni

  1. hyalurononglucosaminidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. cell adhesion Source: UniProtKB-KW
  3. fusion of sperm to egg plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Cell adhesion

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase PH-20 (EC:3.2.1.35)
Short name:
Hyal-PH20
Alternative name(s):
Hyaluronoglucosaminidase PH-20
Sperm adhesion molecule 1
Sperm surface protein PH-20
Gene namesi
Name:SPAM1
Synonyms:PH-20, PH20
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3535Add
BLAST
Chaini36 – 492457Hyaluronidase PH-20PRO_0000012087Add
BLAST
Propeptidei493 – 52937Removed in mature formSequence AnalysisPRO_0000012088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 351By similarity
Glycosylationi81 – 811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi165 – 1651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi179 – 1791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi223 ↔ 237By similarity
Glycosylationi253 – 2531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi376 ↔ 387By similarity
Disulfide bondi381 ↔ 435By similarity
Glycosylationi401 – 4011N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi437 ↔ 464By similarity
Lipidationi492 – 4921GPI-anchor amidated serineSequence Analysis

Post-translational modificationi

Endoproteolysis (toward the C-terminus producing two disulfide-linked fragments) could activate PH-20.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Tissue specificityi

Testis.

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000001550.

Structurei

3D structure databases

ProteinModelPortaliP23613.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG77606.
GeneTreeiENSGT00550000074476.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiP23613.
OMAiQIDHYIS.
OrthoDBiEOG74J97S.
TreeFamiTF321598.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23613-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGAFTFKHSF FGSFVECSGV LQTVFIFLLI PCCLADKRAP PLIPNVPLLW
60 70 80 90 100
VWNAPTEFCI GGTNQPLDMS FFSIVGTPRK NITGQSITLY YVDRLGYYPY
110 120 130 140 150
IDPHTGAIVH GGLPQLMNLQ QHLRKSRQDI LFYMPTDSVG LAVIDWEEWR
160 170 180 190 200
PTWTRNWRPK DIYRNKSIEL VKSQHPQYNH SYAVAVAKRD FERTGKAFML
210 220 230 240 250
ETLKLGKSLR PSSLWGYYLF PDCYNTHFTK PNYDGHCPPI ELQRNNDLQW
260 270 280 290 300
LWNDSTALYP SVYLTSRVRS SQNGALYVRN RVHESIRVSK LMDDKNPLPI
310 320 330 340 350
YVYIRLVFTD QTTTFLELDD LVHSVGEIVP LGVSGIIIWG SLSLTRSLVS
360 370 380 390 400
CIGLENYMKG TLLPYLINVT LAAKMCGQVL CKNQGICTRK DWNTNTYLHL
410 420 430 440 450
NATNFDIELQ QNGKFVVHGK PSLEDLQEFS KNFHCSCYTN VACKDRLDVH
460 470 480 490 500
NVRSVNVCTA NNICIDAVLN FPSLDDDDEP PITDDTSQNQ DSISDITSSA
510 520
PPSSHILPKD LSWCLFLLSI FSQHWKYLL
Length:529
Mass (Da):60,365
Last modified:November 1, 1991 - v1
Checksum:iB3432356AED46245
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56332 mRNA. Translation: CAA39768.1.
PIRiA36343.
RefSeqiNP_001166492.1. NM_001173021.2.

Genome annotation databases

EnsembliENSCPOT00000001737; ENSCPOP00000001550; ENSCPOG00000001716.
GeneIDi100135624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X56332 mRNA. Translation: CAA39768.1 .
PIRi A36343.
RefSeqi NP_001166492.1. NM_001173021.2.

3D structure databases

ProteinModelPortali P23613.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000001550.

Protein family/group databases

CAZyi GH56. Glycoside Hydrolase Family 56.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSCPOT00000001737 ; ENSCPOP00000001550 ; ENSCPOG00000001716 .
GeneIDi 100135624.

Organism-specific databases

CTDi 6677.

Phylogenomic databases

eggNOGi NOG77606.
GeneTreei ENSGT00550000074476.
HOGENOMi HOG000015133.
HOVERGENi HBG052053.
InParanoidi P23613.
OMAi QIDHYIS.
OrthoDBi EOG74J97S.
TreeFami TF321598.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
IPR001439. Hyaluronidase_PH20.
[Graphical view ]
PANTHERi PTHR11769. PTHR11769. 1 hit.
Pfami PF01630. Glyco_hydro_56. 1 hit.
[Graphical view ]
PIRSFi PIRSF038193. Hyaluronidase. 1 hit.
PIRSF500773. Hyaluronidase_PH20_Hyal5. 1 hit.
PRINTSi PR00846. GLHYDRLASE56.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "cDNA cloning reveals the molecular structure of a sperm surface protein, PH-20, involved in sperm-egg adhesion and the wide distribution of its gene among mammals."
    Lathrop W.F., Carmichael E.P., Myles D.G., Primakoff P.
    J. Cell Biol. 111:2939-2949(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: Hartley.

Entry informationi

Entry nameiHYALP_CAVPO
AccessioniPrimary (citable) accession number: P23613
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3