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P23611

- IRF8_MOUSE

UniProt

P23611 - IRF8_MOUSE

Protein

Interferon regulatory factor 8

Gene

Irf8

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi7 – 114108IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: UniProtKB
    3. regulatory region DNA binding Source: InterPro
    4. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. cellular response to lipopolysaccharide Source: MGI
    2. defense response to bacterium Source: MGI
    3. defense response to protozoan Source: MGI
    4. immune response Source: MGI
    5. myeloid cell differentiation Source: MGI
    6. negative regulation of growth of symbiont in host Source: MGI
    7. phagocytosis Source: MGI
    8. positive regulation of interferon-gamma production Source: MGI
    9. positive regulation of interleukin-12 production Source: MGI
    10. positive regulation of transcription, DNA-templated Source: MGI
    11. positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
    12. regulation of transcription, DNA-templated Source: MGI
    13. response to bacterium Source: MGI
    14. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_198660. Interferon gamma signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Interferon regulatory factor 8
    Short name:
    IRF-8
    Alternative name(s):
    Interferon consensus sequence-binding protein
    Short name:
    ICSBP
    Gene namesi
    Name:Irf8
    Synonyms:Icsbp, Icsbp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 8

    Organism-specific databases

    MGIiMGI:96395. Irf8.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 424424Interferon regulatory factor 8PRO_0000154565Add
    BLAST

    Post-translational modificationi

    Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.1 Publication

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    MaxQBiP23611.
    PaxDbiP23611.
    PRIDEiP23611.

    PTM databases

    PhosphoSiteiP23611.

    Expressioni

    Tissue specificityi

    Predominantly in lymphoid tissues.

    Inductioni

    By interferon gamma.1 Publication

    Gene expression databases

    ArrayExpressiP23611.
    BgeeiP23611.
    CleanExiMM_IRF8.
    GenevestigatoriP23611.

    Interactioni

    Subunit structurei

    Interacts with COPS2 By similarity. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.By similarity2 Publications

    Protein-protein interaction databases

    BioGridi200504. 6 interactions.
    IntActiP23611. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP23611.
    SMRiP23611. Positions 9-115, 198-377.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the IRF family.PROSITE-ProRule annotation
    Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG42878.
    HOGENOMiHOG000010107.
    HOVERGENiHBG003072.
    InParanoidiP23611.
    KOiK10155.
    OMAiRELQQFY.
    OrthoDBiEOG7CCBR1.
    PhylomeDBiP23611.
    TreeFamiTF328512.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProiIPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view]
    PRINTSiPR00267. INTFRNREGFCT.
    SMARTiSM00348. IRF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23611-1 [UniParc]FASTAAdd to Basket

    « Hide

    MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ    50
    EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ 100
    LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP 150
    SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS 200
    AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF 250
    PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN 300
    AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE 350
    EFPDTVPLRS KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA 400
    FRMFPDICTS HQRPFFRENQ QITV 424
    Length:424
    Mass (Da):48,237
    Last modified:November 1, 1991 - v1
    Checksum:iFBE79A76846E8EB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32489 mRNA. Translation: AAA37878.1.
    AK018533 mRNA. Translation: BAB31258.1.
    BC005450 mRNA. Translation: AAH05450.1.
    CCDSiCCDS22721.1.
    PIRiA35861.
    RefSeqiNP_032346.1. NM_008320.3.
    XP_006530767.1. XM_006530704.1.
    UniGeneiMm.334861.

    Genome annotation databases

    EnsembliENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515.
    ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515.
    GeneIDi15900.
    KEGGimmu:15900.
    UCSCiuc009nrk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M32489 mRNA. Translation: AAA37878.1 .
    AK018533 mRNA. Translation: BAB31258.1 .
    BC005450 mRNA. Translation: AAH05450.1 .
    CCDSi CCDS22721.1.
    PIRi A35861.
    RefSeqi NP_032346.1. NM_008320.3.
    XP_006530767.1. XM_006530704.1.
    UniGenei Mm.334861.

    3D structure databases

    ProteinModelPortali P23611.
    SMRi P23611. Positions 9-115, 198-377.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200504. 6 interactions.
    IntActi P23611. 1 interaction.

    PTM databases

    PhosphoSitei P23611.

    Proteomic databases

    MaxQBi P23611.
    PaxDbi P23611.
    PRIDEi P23611.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047737 ; ENSMUSP00000040245 ; ENSMUSG00000041515 .
    ENSMUST00000162001 ; ENSMUSP00000125029 ; ENSMUSG00000041515 .
    GeneIDi 15900.
    KEGGi mmu:15900.
    UCSCi uc009nrk.1. mouse.

    Organism-specific databases

    CTDi 3394.
    MGIi MGI:96395. Irf8.

    Phylogenomic databases

    eggNOGi NOG42878.
    HOGENOMi HOG000010107.
    HOVERGENi HBG003072.
    InParanoidi P23611.
    KOi K10155.
    OMAi RELQQFY.
    OrthoDBi EOG7CCBR1.
    PhylomeDBi P23611.
    TreeFami TF328512.

    Enzyme and pathway databases

    Reactomei REACT_198660. Interferon gamma signaling.

    Miscellaneous databases

    NextBioi 288616.
    PROi P23611.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23611.
    Bgeei P23611.
    CleanExi MM_IRF8.
    Genevestigatori P23611.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.10. 1 hit.
    InterProi IPR019817. Interferon_reg_fac_CS.
    IPR001346. Interferon_reg_fact_DNA-bd_dom.
    IPR019471. Interferon_reg_factor-3.
    IPR017855. SMAD_dom-like.
    IPR008984. SMAD_FHA_domain.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00605. IRF. 1 hit.
    PF10401. IRF-3. 1 hit.
    [Graphical view ]
    PRINTSi PR00267. INTFRNREGFCT.
    SMARTi SM00348. IRF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS00601. IRF_1. 1 hit.
    PS51507. IRF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes."
      Driggers P.H., Ennist D.L., Gleason S.L., Mak W.-H., Marks M.S., Levi B.-Z., Flanagan J.R., Appella E., Ozato K.
      Proc. Natl. Acad. Sci. U.S.A. 87:3743-3747(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Colon.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    4. "Autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages."
      Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P., Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K.
      J. Immunol. 179:26-30(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TRIM21, UBIQUITINATION, INDUCTION, SUBCELLULAR LOCATION.
    5. Cited for: FUNCTION, INTERACTION WITH BATF.

    Entry informationi

    Entry nameiIRF8_MOUSE
    AccessioniPrimary (citable) accession number: P23611
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 126 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3