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P23611

- IRF8_MOUSE

UniProt

P23611 - IRF8_MOUSE

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Protein

Interferon regulatory factor 8

Gene
Irf8, Icsbp, Icsbp1
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi7 – 114108IRF tryptophan pentad repeatAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. protein binding Source: UniProtKB
  3. regulatory region DNA binding Source: InterPro
  4. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: MGI
  2. defense response to bacterium Source: MGI
  3. defense response to protozoan Source: MGI
  4. immune response Source: MGI
  5. myeloid cell differentiation Source: MGI
  6. negative regulation of growth of symbiont in host Source: MGI
  7. phagocytosis Source: MGI
  8. positive regulation of interferon-gamma production Source: MGI
  9. positive regulation of interleukin-12 production Source: MGI
  10. positive regulation of transcription, DNA-templated Source: MGI
  11. positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  12. regulation of transcription, DNA-templated Source: MGI
  13. response to bacterium Source: MGI
  14. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_198660. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 8
Short name:
IRF-8
Alternative name(s):
Interferon consensus sequence-binding protein
Short name:
ICSBP
Gene namesi
Name:Irf8
Synonyms:Icsbp, Icsbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 8

Organism-specific databases

MGIiMGI:96395. Irf8.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Interferon regulatory factor 8PRO_0000154565Add
BLAST

Post-translational modificationi

Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP23611.
PaxDbiP23611.
PRIDEiP23611.

PTM databases

PhosphoSiteiP23611.

Expressioni

Tissue specificityi

Predominantly in lymphoid tissues.

Inductioni

By interferon gamma.1 Publication

Gene expression databases

ArrayExpressiP23611.
BgeeiP23611.
CleanExiMM_IRF8.
GenevestigatoriP23611.

Interactioni

Subunit structurei

Interacts with COPS2 By similarity. Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.2 Publications

Protein-protein interaction databases

BioGridi200504. 6 interactions.
IntActiP23611. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP23611.
SMRiP23611. Positions 9-115, 198-377.

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.

Phylogenomic databases

eggNOGiNOG42878.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiP23611.
KOiK10155.
OMAiRELQQFY.
OrthoDBiEOG7CCBR1.
PhylomeDBiP23611.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23611-1 [UniParc]FASTAAdd to Basket

« Hide

MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ    50
EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ 100
LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP 150
SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS 200
AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF 250
PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN 300
AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE 350
EFPDTVPLRS KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA 400
FRMFPDICTS HQRPFFRENQ QITV 424
Length:424
Mass (Da):48,237
Last modified:November 1, 1991 - v1
Checksum:iFBE79A76846E8EB2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32489 mRNA. Translation: AAA37878.1.
AK018533 mRNA. Translation: BAB31258.1.
BC005450 mRNA. Translation: AAH05450.1.
CCDSiCCDS22721.1.
PIRiA35861.
RefSeqiNP_032346.1. NM_008320.3.
XP_006530767.1. XM_006530704.1.
UniGeneiMm.334861.

Genome annotation databases

EnsembliENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515.
ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515.
GeneIDi15900.
KEGGimmu:15900.
UCSCiuc009nrk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M32489 mRNA. Translation: AAA37878.1 .
AK018533 mRNA. Translation: BAB31258.1 .
BC005450 mRNA. Translation: AAH05450.1 .
CCDSi CCDS22721.1.
PIRi A35861.
RefSeqi NP_032346.1. NM_008320.3.
XP_006530767.1. XM_006530704.1.
UniGenei Mm.334861.

3D structure databases

ProteinModelPortali P23611.
SMRi P23611. Positions 9-115, 198-377.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 200504. 6 interactions.
IntActi P23611. 1 interaction.

PTM databases

PhosphoSitei P23611.

Proteomic databases

MaxQBi P23611.
PaxDbi P23611.
PRIDEi P23611.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047737 ; ENSMUSP00000040245 ; ENSMUSG00000041515 .
ENSMUST00000162001 ; ENSMUSP00000125029 ; ENSMUSG00000041515 .
GeneIDi 15900.
KEGGi mmu:15900.
UCSCi uc009nrk.1. mouse.

Organism-specific databases

CTDi 3394.
MGIi MGI:96395. Irf8.

Phylogenomic databases

eggNOGi NOG42878.
HOGENOMi HOG000010107.
HOVERGENi HBG003072.
InParanoidi P23611.
KOi K10155.
OMAi RELQQFY.
OrthoDBi EOG7CCBR1.
PhylomeDBi P23611.
TreeFami TF328512.

Enzyme and pathway databases

Reactomei REACT_198660. Interferon gamma signaling.

Miscellaneous databases

NextBioi 288616.
PROi P23611.
SOURCEi Search...

Gene expression databases

ArrayExpressi P23611.
Bgeei P23611.
CleanExi MM_IRF8.
Genevestigatori P23611.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProi IPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
Pfami PF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view ]
PRINTSi PR00267. INTFRNREGFCT.
SMARTi SM00348. IRF. 1 hit.
[Graphical view ]
SUPFAMi SSF49879. SSF49879. 1 hit.
PROSITEi PS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes."
    Driggers P.H., Ennist D.L., Gleason S.L., Mak W.-H., Marks M.S., Levi B.-Z., Flanagan J.R., Appella E., Ozato K.
    Proc. Natl. Acad. Sci. U.S.A. 87:3743-3747(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Colon.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages."
    Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P., Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K.
    J. Immunol. 179:26-30(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM21, UBIQUITINATION, INDUCTION, SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, INTERACTION WITH BATF.

Entry informationi

Entry nameiIRF8_MOUSE
AccessioniPrimary (citable) accession number: P23611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 3, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi