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Protein

Interferon regulatory factor 8

Gene

Irf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role as a transcriptional activator or repressor (By similarity). Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a negative regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes (PubMed:22992524).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi7 – 114IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd BLAST108

GO - Molecular functioni

GO - Biological processi

  • cellular response to interferon-gamma Source: UniProtKB
  • cellular response to lipopolysaccharide Source: MGI
  • defense response to bacterium Source: MGI
  • defense response to protozoan Source: MGI
  • immune response Source: MGI
  • myeloid cell differentiation Source: MGI
  • negative regulation of growth of symbiont in host Source: MGI
  • negative regulation of transcription by RNA polymerase II Source: MGI
  • phagocytosis Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of interleukin-12 production Source: MGI
  • positive regulation of transcription, DNA-templated Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: MGI
  • positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  • regulation of transcription, DNA-templated Source: MGI
  • response to bacterium Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionActivator, DNA-binding, Repressor
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 8
Short name:
IRF-8
Alternative name(s):
Interferon consensus sequence-binding protein
Short name:
ICSBP
Gene namesi
Name:Irf8
Synonyms:Icsbp, Icsbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96395 Irf8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001545651 – 424Interferon regulatory factor 8Add BLAST424

Post-translational modificationi

Ubiquitinated (PubMed:17579016). Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes (PubMed:17579016). Ubiquitination leads to its degradation by the proteasome (By similarity).By similarity1 Publication
Sumoylated with SUMO3. Desumoylated by SENP1.By similarity

Keywords - PTMi

Ubl conjugation

Proteomic databases

EPDiP23611
PaxDbiP23611
PeptideAtlasiP23611
PRIDEiP23611

PTM databases

iPTMnetiP23611
PhosphoSitePlusiP23611

Expressioni

Tissue specificityi

Predominantly in lymphoid tissues.

Inductioni

By interferon gamma.1 Publication

Gene expression databases

BgeeiENSMUSG00000041515
CleanExiMM_IRF8
ExpressionAtlasiP23611 baseline and differential
GenevisibleiP23611 MM

Interactioni

Subunit structurei

Interacts with COPS2 (By similarity). Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.By similarity2 Publications

Protein-protein interaction databases

BioGridi200504, 5 interactors
IntActiP23611, 1 interactor
STRINGi10090.ENSMUSP00000040245

Structurei

3D structure databases

ProteinModelPortaliP23611
SMRiP23611
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410IED8 Eukaryota
ENOG410XNYR LUCA
GeneTreeiENSGT00760000119093
HOGENOMiHOG000010107
HOVERGENiHBG003072
InParanoidiP23611
KOiK10155
OMAiCPEGCRL
OrthoDBiEOG091G067P
PhylomeDBiP23611
TreeFamiTF328512

Family and domain databases

CDDicd00103 IRF, 1 hit
Gene3Di1.10.10.10, 1 hit
2.60.200.10, 1 hit
InterProiView protein in InterPro
IPR019817 Interferon_reg_fac_CS
IPR001346 Interferon_reg_fact_DNA-bd_dom
IPR019471 Interferon_reg_factor-3
IPR017855 SMAD-like_dom_sf
IPR008984 SMAD_FHA_dom_sf
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR11949 PTHR11949, 1 hit
PfamiView protein in Pfam
PF00605 IRF, 1 hit
PF10401 IRF-3, 1 hit
PRINTSiPR00267 INTFRNREGFCT
SMARTiView protein in SMART
SM00348 IRF, 1 hit
SM01243 IRF-3, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF49879 SSF49879, 1 hit
PROSITEiView protein in PROSITE
PS00601 IRF_1, 1 hit
PS51507 IRF_2, 1 hit

Sequencei

Sequence statusi: Complete.

P23611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ
60 70 80 90 100
EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ
110 120 130 140 150
LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP
160 170 180 190 200
SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS
210 220 230 240 250
AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF
260 270 280 290 300
PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN
310 320 330 340 350
AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE
360 370 380 390 400
EFPDTVPLRS KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA
410 420
FRMFPDICTS HQRPFFRENQ QITV
Length:424
Mass (Da):48,237
Last modified:November 1, 1991 - v1
Checksum:iFBE79A76846E8EB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32489 mRNA Translation: AAA37878.1
AK018533 mRNA Translation: BAB31258.1
BC005450 mRNA Translation: AAH05450.1
CCDSiCCDS22721.1
PIRiA35861
RefSeqiNP_001288740.1, NM_001301811.1
NP_032346.1, NM_008320.4
UniGeneiMm.334861

Genome annotation databases

EnsembliENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515
ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515
GeneIDi15900
KEGGimmu:15900
UCSCiuc009nrk.2 mouse

Similar proteinsi

Entry informationi

Entry nameiIRF8_MOUSE
AccessioniPrimary (citable) accession number: P23611
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 23, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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