Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Interferon regulatory factor 8

Gene

Irf8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)). Plays a regulatory role in cells of the immune system. Involved in CD8+ dendritic cell differentiation by forming a complex with the BATF-JUNB heterodimer in immune cells, leading to recognition of AICE sequence (5'-TGAnTCA/GAAA-3'), an immune-specific regulatory element, followed by cooperative binding of BATF and IRF8 and activation of genes.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi7 – 114108IRF tryptophan pentad repeatPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. regulatory region DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: MGI

GO - Biological processi

  1. cellular response to lipopolysaccharide Source: MGI
  2. defense response to bacterium Source: MGI
  3. defense response to protozoan Source: MGI
  4. immune response Source: MGI
  5. myeloid cell differentiation Source: MGI
  6. negative regulation of growth of symbiont in host Source: MGI
  7. phagocytosis Source: MGI
  8. positive regulation of interferon-gamma production Source: MGI
  9. positive regulation of interleukin-12 production Source: MGI
  10. positive regulation of transcription, DNA-templated Source: MGI
  11. positive regulation of transcription initiation from RNA polymerase II promoter Source: MGI
  12. regulation of transcription, DNA-templated Source: MGI
  13. response to bacterium Source: MGI
  14. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_349056. Interferon gamma signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Interferon regulatory factor 8
Short name:
IRF-8
Alternative name(s):
Interferon consensus sequence-binding protein
Short name:
ICSBP
Gene namesi
Name:Irf8
Synonyms:Icsbp, Icsbp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:96395. Irf8.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. nucleoplasm Source: MGI
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 424424Interferon regulatory factor 8PRO_0000154565Add
BLAST

Post-translational modificationi

Ubiquitinated. Ubiquitination by TRIM21 in macrophages, a process that is strongly increased upon interferon gamma stimulation, leds to the enhanced transcriptional activity of target cytokine genes.1 Publication

Keywords - PTMi

Ubl conjugation

Proteomic databases

MaxQBiP23611.
PaxDbiP23611.
PRIDEiP23611.

PTM databases

PhosphoSiteiP23611.

Expressioni

Tissue specificityi

Predominantly in lymphoid tissues.

Inductioni

By interferon gamma.1 Publication

Gene expression databases

BgeeiP23611.
CleanExiMM_IRF8.
ExpressionAtlasiP23611. baseline and differential.
GenevestigatoriP23611.

Interactioni

Subunit structurei

Interacts with COPS2 (By similarity). Interacts (via C-terminus) with TRIM21 (via C-terminus). Interacts with the BATF-JUNB heterodimer. Interacts with BATF (via bZIP domain); the interaction is direct.By similarity2 Publications

Protein-protein interaction databases

BioGridi200504. 6 interactions.
IntActiP23611. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP23611.
SMRiP23611. Positions 9-115, 198-377.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IRF family.PROSITE-ProRule annotation
Contains 1 IRF tryptophan pentad repeat DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG42878.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiP23611.
KOiK10155.
OMAiRELQQFY.
OrthoDBiEOG7CCBR1.
PhylomeDBiP23611.
TreeFamiTF328512.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23611-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCDRNGGRRL RQWLIEQIDS SMYPGLIWEN DEKTMFRIPW KHAGKQDYNQ
60 70 80 90 100
EVDASIFKAW AVFKGKFKEG DKAEPATWKT RLRCALNKSP DFEEVTDRSQ
110 120 130 140 150
LDISEPYKVY RIVPEEEQKC KLGVAPAGCM SEVPEMECGR SEIEELIKEP
160 170 180 190 200
SVDEYMGMTK RSPSPPEACR SQILPDWWVQ QPSAGLPLVT GYAAYDTHHS
210 220 230 240 250
AFSQMVISFY YGGKLVGQAT TTCLEGCRLS LSQPGLPKLY GPDGLEPVCF
260 270 280 290 300
PTADTIPSER QRQVTRKLFG HLERGVLLHS NRKGVFVKRL CQGRVFCSGN
310 320 330 340 350
AVVCKGRPNK LERDEVVQVF DTNQFIRELQ QFYATQSRLP DSRVVLCFGE
360 370 380 390 400
EFPDTVPLRS KLILVQVEQL YARQLVEEAG KSCGAGSLMP ALEEPQPDQA
410 420
FRMFPDICTS HQRPFFRENQ QITV
Length:424
Mass (Da):48,237
Last modified:October 31, 1991 - v1
Checksum:iFBE79A76846E8EB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32489 mRNA. Translation: AAA37878.1.
AK018533 mRNA. Translation: BAB31258.1.
BC005450 mRNA. Translation: AAH05450.1.
CCDSiCCDS22721.1.
PIRiA35861.
RefSeqiNP_001288740.1. NM_001301811.1.
NP_032346.1. NM_008320.4.
UniGeneiMm.334861.

Genome annotation databases

EnsembliENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515.
ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515.
GeneIDi15900.
KEGGimmu:15900.
UCSCiuc009nrk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M32489 mRNA. Translation: AAA37878.1.
AK018533 mRNA. Translation: BAB31258.1.
BC005450 mRNA. Translation: AAH05450.1.
CCDSiCCDS22721.1.
PIRiA35861.
RefSeqiNP_001288740.1. NM_001301811.1.
NP_032346.1. NM_008320.4.
UniGeneiMm.334861.

3D structure databases

ProteinModelPortaliP23611.
SMRiP23611. Positions 9-115, 198-377.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200504. 6 interactions.
IntActiP23611. 1 interaction.

PTM databases

PhosphoSiteiP23611.

Proteomic databases

MaxQBiP23611.
PaxDbiP23611.
PRIDEiP23611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047737; ENSMUSP00000040245; ENSMUSG00000041515.
ENSMUST00000162001; ENSMUSP00000125029; ENSMUSG00000041515.
GeneIDi15900.
KEGGimmu:15900.
UCSCiuc009nrk.1. mouse.

Organism-specific databases

CTDi3394.
MGIiMGI:96395. Irf8.

Phylogenomic databases

eggNOGiNOG42878.
HOGENOMiHOG000010107.
HOVERGENiHBG003072.
InParanoidiP23611.
KOiK10155.
OMAiRELQQFY.
OrthoDBiEOG7CCBR1.
PhylomeDBiP23611.
TreeFamiTF328512.

Enzyme and pathway databases

ReactomeiREACT_349056. Interferon gamma signaling.

Miscellaneous databases

NextBioi288616.
PROiP23611.
SOURCEiSearch...

Gene expression databases

BgeeiP23611.
CleanExiMM_IRF8.
ExpressionAtlasiP23611. baseline and differential.
GenevestigatoriP23611.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProiIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSiPR00267. INTFRNREGFCT.
SMARTiSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMiSSF49879. SSF49879. 1 hit.
PROSITEiPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "An interferon gamma-regulated protein that binds the interferon-inducible enhancer element of major histocompatibility complex class I genes."
    Driggers P.H., Ennist D.L., Gleason S.L., Mak W.-H., Marks M.S., Levi B.-Z., Flanagan J.R., Appella E., Ozato K.
    Proc. Natl. Acad. Sci. U.S.A. 87:3743-3747(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Colon.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  4. "Autoantigen Ro52 is an interferon inducible E3 ligase that ubiquitinates IRF-8 and enhances cytokine expression in macrophages."
    Kong H.J., Anderson D.E., Lee C.H., Jang M.K., Tamura T., Tailor P., Cho H.K., Cheong J., Xiong H., Morse H.C. III, Ozato K.
    J. Immunol. 179:26-30(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRIM21, UBIQUITINATION, INDUCTION, SUBCELLULAR LOCATION.
  5. Cited for: FUNCTION, INTERACTION WITH BATF.

Entry informationi

Entry nameiIRF8_MOUSE
AccessioniPrimary (citable) accession number: P23611
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: October 31, 1991
Last modified: March 31, 2015
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.