Reviewed, UniProtKB/Swiss-Prot P23599 (1A11_CUCMA)

Names and origin

Protein names

Recommended name:
    1-aminocyclopropane-1-carboxylate synthase CMW33
      Short name=ACC synthase
    EC=4.4.1.14
Alternative name(s):
    S-adenosyl-L-methionine methylthioadenosine-lyase

Gene names

Name:	ACS1
Synonyms:	ACCW

Organism

Cucurbita maxima (Pumpkin) (Winter squash)

Taxonomic identifier

3661 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucurbita

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants.
Catalytic activity	S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.
Cofactor	Pyridoxal phosphate.
Pathway	Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.
Subunit structure	Homodimer.
Induction	By tissue wounding.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Ethylene biosynthesis Fruit ripening
Ligand	Pyridoxal phosphate S-adenosyl-L-methionine
Molecular function	Lyase
Gene Ontology (GO)
Biological process	ethylene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	1-aminocyclopropane-1-carboxylate synthase CMW33		PRO_0000123907
Amino acid modifications
	Modified residue	279	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23599-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: F39234AC99CBEF6B	FASTA	493	55,896
	10 20 30 40 50 60 MEFHQIDERN QALLSKIAVD DGHGENSPYF DGWKAYDNDP FHPEDNPLGV IQMGLAENQL 70 80 90 100 110 120 SFDMIVDWIR KHPEASICTP KGLERFKSIA NFQDYHGLPE FRNGIASFMG KVRGGRVQFD 130 140 150 160 170 180 PSRIVMGGGA TGASETVIFC LADPGDAFLV PSPYYAAFDR DLKWRTRAQI IRVHCNSSNN 190 200 210 220 230 240 FQVTKAALEI AYKKAQEANI KVKGVIITNP SNPLGTTYDR DTLKTLVTFV NQHDIHLICD 250 260 270 280 290 300 EIYSATVFKA PTFISIAQIV EEMEHCKKEL IHILYSLSKD MGLPGFRVGI IYSYNDVVVR 310 320 330 340 350 360 RARQMSSFGL VSSQTQHLLA AMLSDEDFVD KFLAENSKRL AERHARFTKE LDKMGITCLN 370 380 390 400 410 420 SNAGVFVWMD LRRLLKDQTF KAEMELWRVI INEVKLNVSP GSSFHVTEPG WFRVCFANMD 430 440 450 460 470 480 DNTVDVALNR IHSFVENIDK KEDNTVAMPS KTRRRENKLR LSFSFSGRRY DEGNVLNSPH 490 TMSPHSPLVI AKN

References

[1]	"Molecular cloning and sequence of a complementary DNA encoding 1-aminocyclopropane-1-carboxylate synthase induced by tissue wounding." Nakajima N., Mori H., Yamazaki K., Imaseki H. Plant Cell Physiol. 31:1021-1029(1990) Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	D01032 mRNA. Translation: BAA00838.1.
PIR	JQ0926.
3D structure databases
HSSP	HSSP built from PDB template 1IAX based on UniProtKB P18485.
SMR	P23599. Positions 18-435.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.14. 2176.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1A11_CUCMA

Accession

Primary (citable) accession number: P23599

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 22, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)