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Protein

Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

Gene

PPH21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Exact function not known, phosphatase 2A performs an essential cellular function.

Catalytic activityi

[a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117Manganese 1By similarity1
Metal bindingi119Manganese 1By similarity1
Metal bindingi145Manganese 1By similarity1
Metal bindingi145Manganese 2By similarity1
Metal bindingi177Manganese 2By similarity1
Active sitei178Proton donorBy similarity1
Metal bindingi227Manganese 2By similarity1
Metal bindingi301Manganese 2By similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • protein serine/threonine phosphatase activity Source: SGD

GO - Biological processi

  • actin filament organization Source: SGD
  • budding cell bud growth Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • mitotic spindle assembly checkpoint Source: SGD
  • peptidyl-serine dephosphorylation Source: SGD
  • protein dephosphorylation Source: SGD
  • regulation of translation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-29532-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (EC:3.1.3.16)
Gene namesi
Name:PPH21
Ordered Locus Names:YDL134C
ORF Names:D2180
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDL134C.
SGDiS000002292. PPH21.

Subcellular locationi

GO - Cellular componenti

  • cytoplasmic stress granule Source: SGD
  • protein phosphatase type 2A complex Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi99L → A: Reduced interaction with TAP42. 1 Publication1
Mutagenesisi102E → A: Reduced interaction with TAP42. 1 Publication1
Mutagenesisi103E → A: Reduced interaction with TAP42. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000588731 – 369Serine/threonine-protein phosphatase PP2A-1 catalytic subunitAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei369Leucine methyl ester1 Publication1

Post-translational modificationi

Reversibly methyl esterified on Leu-369 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.2 Publications

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP23594.
PRIDEiP23594.

PTM databases

iPTMnetiP23594.

Interactioni

Subunit structurei

Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD2, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD2-TAP42.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TAP42Q043726EBI-12745,EBI-18926

Protein-protein interaction databases

BioGridi31927. 145 interactors.
DIPiDIP-2282N.
IntActiP23594. 13 interactors.
MINTiMINT-534242.

Structurei

3D structure databases

ProteinModelPortaliP23594.
SMRiP23594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
InParanoidiP23594.
KOiK04382.
OMAiEIFKCAG.
OrthoDBiEOG092C2R40.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG
60 70 80 90 100
IADHKSSKPL ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ
110 120 130 140 150
FEENVKPINV PVTICGDVHG QFHDLLELFK IGGPCPDTNY LFMGDYVDRG
160 170 180 190 200
YYSVETVSYL VAMKVRYPHR ITILRGNHES RQITQVYGFY DECLRKYGSA
210 220 230 240 250
NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV RELNRIQEVP
260 270 280 290 300
HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA
310 320 330 340 350
HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD
360
PSVRPGEPSV SRKTPDYFL
Length:369
Mass (Da):41,938
Last modified:November 1, 1991 - v1
Checksum:iD8D5757283C9BBD6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56261 Genomic DNA. Translation: CAA39702.1.
X58856 Genomic DNA. Translation: CAA41656.1.
X96876 Genomic DNA. Translation: CAA65625.1.
Z74182 Genomic DNA. Translation: CAA98707.1.
BK006938 Genomic DNA. Translation: DAA11724.1.
PIRiA41525. PABY21.
RefSeqiNP_010147.1. NM_001180193.1.

Genome annotation databases

EnsemblFungiiYDL134C; YDL134C; YDL134C.
GeneIDi851421.
KEGGisce:YDL134C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56261 Genomic DNA. Translation: CAA39702.1.
X58856 Genomic DNA. Translation: CAA41656.1.
X96876 Genomic DNA. Translation: CAA65625.1.
Z74182 Genomic DNA. Translation: CAA98707.1.
BK006938 Genomic DNA. Translation: DAA11724.1.
PIRiA41525. PABY21.
RefSeqiNP_010147.1. NM_001180193.1.

3D structure databases

ProteinModelPortaliP23594.
SMRiP23594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31927. 145 interactors.
DIPiDIP-2282N.
IntActiP23594. 13 interactors.
MINTiMINT-534242.

PTM databases

iPTMnetiP23594.

Proteomic databases

MaxQBiP23594.
PRIDEiP23594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYDL134C; YDL134C; YDL134C.
GeneIDi851421.
KEGGisce:YDL134C.

Organism-specific databases

EuPathDBiFungiDB:YDL134C.
SGDiS000002292. PPH21.

Phylogenomic databases

GeneTreeiENSGT00550000074618.
HOGENOMiHOG000172696.
InParanoidiP23594.
KOiK04382.
OMAiEIFKCAG.
OrthoDBiEOG092C2R40.

Enzyme and pathway databases

BioCyciYEAST:G3O-29532-MONOMER.

Miscellaneous databases

PROiP23594.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR004843. Calcineurin-like_PHP_ApaH.
IPR029052. Metallo-depent_PP-like.
IPR006186. Ser/Thr-sp_prot-phosphatase.
[Graphical view]
PfamiPF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR00114. STPHPHTASE.
SMARTiSM00156. PP2Ac. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPP2A1_YEAST
AccessioniPrimary (citable) accession number: P23594
Secondary accession number(s): D6VRL4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5620 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.