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P23594

- PP2A1_YEAST

UniProt

P23594 - PP2A1_YEAST

Protein

Serine/threonine-protein phosphatase PP2A-1 catalytic subunit

Gene

PPH21

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Exact function not known, phosphatase 2A performs an essential cellular function.

    Catalytic activityi

    [a protein]-serine/threonine phosphate + H2O = [a protein]-serine/threonine + phosphate.

    Cofactori

    Binds 2 manganese ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi117 – 1171Manganese 1By similarity
    Metal bindingi119 – 1191Manganese 1By similarity
    Metal bindingi145 – 1451Manganese 1By similarity
    Metal bindingi145 – 1451Manganese 2By similarity
    Metal bindingi177 – 1771Manganese 2By similarity
    Active sitei178 – 1781Proton donorBy similarity
    Metal bindingi227 – 2271Manganese 2By similarity
    Metal bindingi301 – 3011Manganese 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: SGD

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. budding cell bud growth Source: SGD
    3. G1/S transition of mitotic cell cycle Source: SGD
    4. mitotic spindle assembly checkpoint Source: SGD
    5. protein dephosphorylation Source: SGD
    6. regulation of translation Source: SGD

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-29532-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein phosphatase PP2A-1 catalytic subunit (EC:3.1.3.16)
    Gene namesi
    Name:PPH21
    Ordered Locus Names:YDL134C
    ORF Names:D2180
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome IV

    Organism-specific databases

    CYGDiYDL134c.
    SGDiS000002292. PPH21.

    Subcellular locationi

    GO - Cellular componenti

    1. protein phosphatase type 2A complex Source: SGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi99 – 991L → A: Reduced interaction with TAP42. 1 Publication
    Mutagenesisi102 – 1021E → A: Reduced interaction with TAP42. 1 Publication
    Mutagenesisi103 – 1031E → A: Reduced interaction with TAP42. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Serine/threonine-protein phosphatase PP2A-1 catalytic subunitPRO_0000058873Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei369 – 3691Leucine methyl ester2 Publications

    Post-translational modificationi

    Reversibly methyl esterified on Leu-369 by leucine carboxyl methyltransferase 1 (PPM1) and protein phosphatase methylesterase 1 (PPE1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.2 Publications

    Keywords - PTMi

    Methylation

    Proteomic databases

    MaxQBiP23594.
    PaxDbiP23594.
    PeptideAtlasiP23594.

    Expressioni

    Gene expression databases

    GenevestigatoriP23594.

    Interactioni

    Subunit structurei

    Inactivated in a complex with phosphatase methylesterase PPE1 (PP2Ai). Interacts with phosphatase 2A activator RRD2, which can reactivate PP2Ai by dissociating the catalytic subunit from the complex. Forms a ternary complex with RRD2-TAP42.3 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    TAP42Q043726EBI-12745,EBI-18926

    Protein-protein interaction databases

    BioGridi31927. 132 interactions.
    DIPiDIP-2282N.
    IntActiP23594. 13 interactions.
    MINTiMINT-534242.
    STRINGi4932.YDL134C.

    Structurei

    3D structure databases

    ProteinModelPortaliP23594.
    SMRiP23594. Positions 32-357.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PPP phosphatase family. PP-2A subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG0639.
    GeneTreeiENSGT00550000074618.
    HOGENOMiHOG000172696.
    KOiK04382.
    OMAiGVECAAS.
    OrthoDBiEOG7FFN29.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view]
    PfamiPF00149. Metallophos. 1 hit.
    [Graphical view]
    PRINTSiPR00114. STPHPHTASE.
    SMARTiSM00156. PP2Ac. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.
    PROSITEiPS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23594-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDTDLDVPMQ DAVTEQLTPT VSEDMDLNNN SSDNNAEEFS VDDLKPGSSG    50
    IADHKSSKPL ELNNTNINQL DQWIEHLSKC EPLSEDDVAR LCKMAVDVLQ 100
    FEENVKPINV PVTICGDVHG QFHDLLELFK IGGPCPDTNY LFMGDYVDRG 150
    YYSVETVSYL VAMKVRYPHR ITILRGNHES RQITQVYGFY DECLRKYGSA 200
    NVWKMFTDLF DYFPITALVD NKIFCLHGGL SPMIETIDQV RELNRIQEVP 250
    HEGPMCDLLW SDPDDRGGWG ISPRGAGFTF GQDVSEQFNH TNDLSLIARA 300
    HQLVMEGYAW SHQQNVVTIF SAPNYCYRCG NQAAIMEVDE NHNRQFLQYD 350
    PSVRPGEPSV SRKTPDYFL 369
    Length:369
    Mass (Da):41,938
    Last modified:November 1, 1991 - v1
    Checksum:iD8D5757283C9BBD6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56261 Genomic DNA. Translation: CAA39702.1.
    X58856 Genomic DNA. Translation: CAA41656.1.
    X96876 Genomic DNA. Translation: CAA65625.1.
    Z74182 Genomic DNA. Translation: CAA98707.1.
    BK006938 Genomic DNA. Translation: DAA11724.1.
    PIRiA41525. PABY21.
    RefSeqiNP_010147.1. NM_001180193.1.

    Genome annotation databases

    EnsemblFungiiYDL134C; YDL134C; YDL134C.
    GeneIDi851421.
    KEGGisce:YDL134C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56261 Genomic DNA. Translation: CAA39702.1 .
    X58856 Genomic DNA. Translation: CAA41656.1 .
    X96876 Genomic DNA. Translation: CAA65625.1 .
    Z74182 Genomic DNA. Translation: CAA98707.1 .
    BK006938 Genomic DNA. Translation: DAA11724.1 .
    PIRi A41525. PABY21.
    RefSeqi NP_010147.1. NM_001180193.1.

    3D structure databases

    ProteinModelPortali P23594.
    SMRi P23594. Positions 32-357.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31927. 132 interactions.
    DIPi DIP-2282N.
    IntActi P23594. 13 interactions.
    MINTi MINT-534242.
    STRINGi 4932.YDL134C.

    Proteomic databases

    MaxQBi P23594.
    PaxDbi P23594.
    PeptideAtlasi P23594.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YDL134C ; YDL134C ; YDL134C .
    GeneIDi 851421.
    KEGGi sce:YDL134C.

    Organism-specific databases

    CYGDi YDL134c.
    SGDi S000002292. PPH21.

    Phylogenomic databases

    eggNOGi COG0639.
    GeneTreei ENSGT00550000074618.
    HOGENOMi HOG000172696.
    KOi K04382.
    OMAi GVECAAS.
    OrthoDBi EOG7FFN29.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-29532-MONOMER.

    Miscellaneous databases

    NextBioi 968624.

    Gene expression databases

    Genevestigatori P23594.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR004843. Calcineurin-like_PHP_apaH.
    IPR029052. Metallo-depent_PP-like.
    IPR006186. Ser/Thr-sp_prot-phosphatase.
    [Graphical view ]
    Pfami PF00149. Metallophos. 1 hit.
    [Graphical view ]
    PRINTSi PR00114. STPHPHTASE.
    SMARTi SM00156. PP2Ac. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    PROSITEi PS00125. SER_THR_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Saccharomyces cerevisiae protein phosphatase 2A performs an essential cellular function and is encoded by two genes."
      Sneddon A.A., Cohen P.T.W., Stark M.J.R.
      EMBO J. 9:4339-4346(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: LL20.
    2. "Protein phosphatase 2A in Saccharomyces cerevisiae: effects on cell growth and bud morphogenesis."
      Ronne H., Carlberg M., Hu G.-Z., Nehlin J.O.
      Mol. Cell. Biol. 11:4876-4884(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 208353 / W303-1A.
    3. "Analysis of a 26,756 bp segment from the left arm of yeast chromosome IV."
      Woelfl S., Haneman V., Saluz H.P.
      Yeast 12:1549-1554(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    4. "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV."
      Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G., Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C., Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F., Delaveau T.
      , del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M., Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T., Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C., Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S., Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L., Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H., Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M., Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M., Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A., Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G., Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E., Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S., Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D., Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V., Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E., Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M., Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D., Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X., Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A., Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T., Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L., Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E., Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L., Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M., Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K., Mewes H.-W., Zollner A., Zaccaria P.
      Nature 387:75-78(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. "Carboxyl methylation of the phosphoprotein phosphatase 2A catalytic subunit promotes its functional association with regulatory subunits in vivo."
      Wu J., Tolstykh T., Lee J., Boyd K., Stock J.B., Broach J.R.
      EMBO J. 19:5672-5681(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION AT LEU-369 BY PPM1, DEMETHYLATION AT LEU-369 BY PPE1.
    7. "Protein phosphatase methyltransferase 1 (Ppm1p) is the sole activity responsible for modification of the major forms of protein phosphatase 2A in yeast."
      Kalhor H.R., Luk K., Ramos A., Zobel-Thropp P., Clarke S.
      Arch. Biochem. Biophys. 395:239-245(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: METHYLATION BY PPM1.
    8. "Interaction with Tap42 is required for the essential function of Sit4 and type 2A phosphatases."
      Wang H., Wang X., Jiang Y.
      Mol. Biol. Cell 14:4342-4351(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH TAP42, MUTAGENESIS OF LEU-99; GLU-102 AND GLU-103.
    9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    10. "Specific interactions of PP2A and PP2A-like phosphatases with the yeast PTPA homologues, Ypa1 and Ypa2."
      Van Hoof C., Martens E., Longin S., Jordens J., Stevens I., Janssens V., Goris J.
      Biochem. J. 386:93-102(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH PPE1 AND RRD2.
    11. "The yeast phosphotyrosyl phosphatase activator is part of the Tap42-phosphatase complexes."
      Zheng Y., Jiang Y.
      Mol. Biol. Cell 16:2119-2127(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RRD2 AND TAP42.

    Entry informationi

    Entry nameiPP2A1_YEAST
    AccessioniPrimary (citable) accession number: P23594
    Secondary accession number(s): D6VRL4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 5620 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome IV
      Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names

    External Data

    Dasty 3