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Protein

GDP-L-fucose synthase

Gene

Tsta3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.By similarity

Miscellaneous

Mutagen treatment of P815 tumor cells produces tum-variants that elicit a cytolytic T-lymphocyte response (CTL). The antigenic allele differs from the normal allele by a single mutation in position 139.

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.

Pathwayi: GDP-L-fucose biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GDP-mannose 4,6 dehydratase (Gmds)
  2. GDP-L-fucose synthase (Tsta3)
This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose, the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei114Important for catalytic activityBy similarity1
Sitei116Important for catalytic activityBy similarity1
Active sitei143Proton donor/acceptorBy similarity1
Binding sitei147NADPBy similarity1
Sitei147Lowers pKa of active site TyrBy similarity1
Binding sitei186NADPBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei208SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei277SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADPBy similarity7
Nucleotide bindingi170 – 173NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • 'de novo' GDP-L-fucose biosynthetic process Source: MGI
  • cytolysis Source: MGI
  • GDP-mannose metabolic process Source: MGI

Keywordsi

Molecular functionIsomerase, Multifunctional enzyme, Oxidoreductase, Tumor antigen
LigandNADP

Enzyme and pathway databases

ReactomeiR-MMU-6787639 GDP-fucose biosynthesis
UniPathwayiUPA00128; UER00191

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-L-fucose synthase (EC:1.1.1.271)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
Protein FX
Red cell NADP(H)-binding protein
Transplantation antigen P35B
Tum-P35B antigen
Gene namesi
Name:Tsta3
Synonyms:P35b, Tstap35b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:98857 Tsta3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743511 – 321GDP-L-fucose synthaseAdd BLAST321

Proteomic databases

EPDiP23591
PaxDbiP23591
PeptideAtlasiP23591
PRIDEiP23591

PTM databases

iPTMnetiP23591
PhosphoSitePlusiP23591

Expressioni

Gene expression databases

BgeeiENSMUSG00000022570
CleanExiMM_TSTA3
ExpressionAtlasiP23591 baseline and differential
GenevisibleiP23591 MM

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP23591, 2 interactors
MINTiP23591
STRINGi10090.ENSMUSP00000023231

Structurei

3D structure databases

ProteinModelPortaliP23591
SMRiP23591
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1431 Eukaryota
COG0451 LUCA
GeneTreeiENSGT00390000004681
HOGENOMiHOG000168011
HOVERGENiHBG000059
InParanoidiP23591
KOiK02377
OMAiIHCAGRV
OrthoDBiEOG091G0CG3
PhylomeDBiP23591
TreeFamiTF314936

Family and domain databases

CDDicd05239 GDP_FS_SDR_e, 1 hit
HAMAPiMF_00956 GDP_fucose_synth, 1 hit
InterProiView protein in InterPro
IPR001509 Epimerase_deHydtase
IPR028614 GDP_fucose/colitose_synth
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF01370 Epimerase, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit

Sequencei

Sequence statusi: Complete.

P23591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPHGSMRI LVTGGSGLVG RAIQKVVADG AGLPGEEWVF VSSKDADLTD
60 70 80 90 100
AAQTQALFQK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHINDNVLHS
110 120 130 140 150
AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHSSN FGYSYAKRMI
160 170 180 190 200
DVQNRAYFQQ HGCTFTAVIP TNVFGPYDNF NIEDGHVLPG LIHKVHLAKS
210 220 230 240 250
SDSALTVWGT GKPRRQFIYS LDLARLFIWV LREYSEVEPI ILSVGEEDEV
260 270 280 290 300
SIKEAAEAVV EAMDFNGEVT FDSTKSDGQY KKTASNGKLR SYLPDFRFTP
310 320
FKQAVKETCT WFTDNYEQAR K
Length:321
Mass (Da):35,878
Last modified:May 27, 2002 - v3
Checksum:i358D86D68F173531
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti139S → N in allele TUM-. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53620
, X53621, X53622, X53623, X53624, X53625, X53626, X53627, X53628 Genomic DNA Translation: CAB94217.1 Sequence problems.
M30127 mRNA Translation: AAA39673.2
M30128 Genomic DNA Translation: AAA39674.1 Frameshift.
AK029632 mRNA Translation: BAC26539.1
CCDSiCCDS27555.1
PIRiS12516
RefSeqiNP_112478.1, NM_031201.1
XP_006520814.1, XM_006520751.2
UniGeneiMm.22596

Genome annotation databases

EnsembliENSMUST00000023231; ENSMUSP00000023231; ENSMUSG00000022570
GeneIDi22122
KEGGimmu:22122
UCSCiuc007whs.1 mouse

Similar proteinsi

Entry informationi

Entry nameiFCL_MOUSE
AccessioniPrimary (citable) accession number: P23591
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 27, 2002
Last modified: May 23, 2018
This is version 151 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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