Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GDP-L-fucose synthase

Gene

Tsta3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.By similarity

Catalytic activityi

GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH.

Pathwayi: GDP-L-fucose biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. GDP-mannose 4,6 dehydratase (Gmds)
  2. GDP-L-fucose synthase (Tsta3)
This subpathway is part of the pathway GDP-L-fucose biosynthesis via de novo pathway, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GDP-L-fucose from GDP-alpha-D-mannose, the pathway GDP-L-fucose biosynthesis via de novo pathway and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei114Important for catalytic activityBy similarity1
Sitei116Important for catalytic activityBy similarity1
Active sitei143Proton donor/acceptorBy similarity1
Binding sitei147NADPBy similarity1
Sitei147Lowers pKa of active site TyrBy similarity1
Binding sitei186NADPBy similarity1
Binding sitei194SubstrateBy similarity1
Binding sitei208SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei277SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi14 – 20NADPBy similarity7
Nucleotide bindingi170 – 173NADPBy similarity4

GO - Molecular functioni

GO - Biological processi

  • 'de novo' GDP-L-fucose biosynthetic process Source: MGI
  • cytolysis Source: MGI
  • GDP-mannose metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Oxidoreductase, Tumor antigen

Keywords - Ligandi

NADP

Enzyme and pathway databases

ReactomeiR-MMU-6787639. GDP-fucose biosynthesis.
UniPathwayiUPA00128; UER00191.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-L-fucose synthase (EC:1.1.1.271)
Alternative name(s):
GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase
Protein FX
Red cell NADP(H)-binding protein
Transplantation antigen P35B
Tum-P35B antigen
Gene namesi
Name:Tsta3
Synonyms:P35b, Tstap35b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:98857. Tsta3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001743511 – 321GDP-L-fucose synthaseAdd BLAST321

Proteomic databases

EPDiP23591.
PaxDbiP23591.
PeptideAtlasiP23591.
PRIDEiP23591.

PTM databases

iPTMnetiP23591.
PhosphoSitePlusiP23591.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022570.
CleanExiMM_TSTA3.
ExpressionAtlasiP23591. baseline and differential.
GenevisibleiP23591. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

IntActiP23591. 2 interactors.
MINTiMINT-1856547.
STRINGi10090.ENSMUSP00000023231.

Structurei

3D structure databases

ProteinModelPortaliP23591.
SMRiP23591.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1431. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00390000004681.
HOGENOMiHOG000168011.
HOVERGENiHBG000059.
InParanoidiP23591.
KOiK02377.
OMAiRMHTAKL.
OrthoDBiEOG091G0CG3.
PhylomeDBiP23591.
TreeFamiTF314936.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P23591-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEPHGSMRI LVTGGSGLVG RAIQKVVADG AGLPGEEWVF VSSKDADLTD
60 70 80 90 100
AAQTQALFQK VQPTHVIHLA AMVGGLFRNI KYNLDFWRKN VHINDNVLHS
110 120 130 140 150
AFEVGARKVV SCLSTCIFPD KTTYPIDETM IHNGPPHSSN FGYSYAKRMI
160 170 180 190 200
DVQNRAYFQQ HGCTFTAVIP TNVFGPYDNF NIEDGHVLPG LIHKVHLAKS
210 220 230 240 250
SDSALTVWGT GKPRRQFIYS LDLARLFIWV LREYSEVEPI ILSVGEEDEV
260 270 280 290 300
SIKEAAEAVV EAMDFNGEVT FDSTKSDGQY KKTASNGKLR SYLPDFRFTP
310 320
FKQAVKETCT WFTDNYEQAR K
Length:321
Mass (Da):35,878
Last modified:May 27, 2002 - v3
Checksum:i358D86D68F173531
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti139S → N in allele TUM-. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53620
, X53621, X53622, X53623, X53624, X53625, X53626, X53627, X53628 Genomic DNA. Translation: CAB94217.1. Sequence problems.
M30127 mRNA. Translation: AAA39673.2.
M30128 Genomic DNA. Translation: AAA39674.1. Frameshift.
AK029632 mRNA. Translation: BAC26539.1.
CCDSiCCDS27555.1.
PIRiS12516.
RefSeqiNP_112478.1. NM_031201.1.
XP_006520814.1. XM_006520751.2.
UniGeneiMm.22596.

Genome annotation databases

EnsembliENSMUST00000023231; ENSMUSP00000023231; ENSMUSG00000022570.
GeneIDi22122.
KEGGimmu:22122.
UCSCiuc007whs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53620
, X53621, X53622, X53623, X53624, X53625, X53626, X53627, X53628 Genomic DNA. Translation: CAB94217.1. Sequence problems.
M30127 mRNA. Translation: AAA39673.2.
M30128 Genomic DNA. Translation: AAA39674.1. Frameshift.
AK029632 mRNA. Translation: BAC26539.1.
CCDSiCCDS27555.1.
PIRiS12516.
RefSeqiNP_112478.1. NM_031201.1.
XP_006520814.1. XM_006520751.2.
UniGeneiMm.22596.

3D structure databases

ProteinModelPortaliP23591.
SMRiP23591.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP23591. 2 interactors.
MINTiMINT-1856547.
STRINGi10090.ENSMUSP00000023231.

PTM databases

iPTMnetiP23591.
PhosphoSitePlusiP23591.

Proteomic databases

EPDiP23591.
PaxDbiP23591.
PeptideAtlasiP23591.
PRIDEiP23591.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023231; ENSMUSP00000023231; ENSMUSG00000022570.
GeneIDi22122.
KEGGimmu:22122.
UCSCiuc007whs.1. mouse.

Organism-specific databases

CTDi7264.
MGIiMGI:98857. Tsta3.

Phylogenomic databases

eggNOGiKOG1431. Eukaryota.
COG0451. LUCA.
GeneTreeiENSGT00390000004681.
HOGENOMiHOG000168011.
HOVERGENiHBG000059.
InParanoidiP23591.
KOiK02377.
OMAiRMHTAKL.
OrthoDBiEOG091G0CG3.
PhylomeDBiP23591.
TreeFamiTF314936.

Enzyme and pathway databases

UniPathwayiUPA00128; UER00191.
ReactomeiR-MMU-6787639. GDP-fucose biosynthesis.

Miscellaneous databases

PROiP23591.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022570.
CleanExiMM_TSTA3.
ExpressionAtlasiP23591. baseline and differential.
GenevisibleiP23591. MM.

Family and domain databases

CDDicd05239. GDP_FS_SDR_e. 1 hit.
Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00956. GDP_fucose_synth. 1 hit.
InterProiIPR001509. Epimerase_deHydtase.
IPR028614. GDP_fucose/colitose_synth.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFCL_MOUSE
AccessioniPrimary (citable) accession number: P23591
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 27, 2002
Last modified: November 30, 2016
This is version 144 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutagen treatment of P815 tumor cells produces tum-variants that elicit a cytolytic T-lymphocyte response (CTL). The antigenic allele differs from the normal allele by a single mutation in position 139.

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.