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Protein

Eukaryotic translation initiation factor 4B

Gene

EIF4B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

GO - Molecular functioni

  • helicase activity Source: Reactome
  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • ribosomal small subunit binding Source: GO_Central
  • RNA binding Source: ProtInc
  • RNA strand annealing activity Source: GO_Central
  • RNA strand-exchange activity Source: GO_Central
  • translation initiation factor activity Source: ProtInc

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-166208. mTORC1-mediated signalling.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiP23588.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 4B
Short name:
eIF-4B
Gene namesi
Name:EIF4B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:3285. EIF4B.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: Reactome
  • eukaryotic translation initiation factor 4F complex Source: ProtInc
  • polysome Source: GO_Central
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA27713.

Polymorphism and mutation databases

BioMutaiEIF4B.
DMDMi205371761.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 611611Eukaryotic translation initiation factor 4BPRO_0000081616Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei93 – 931PhosphoserineCombined sources
Modified residuei192 – 1921PhosphoserineCombined sources
Modified residuei207 – 2071PhosphoserineCombined sources
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei283 – 2831PhosphoserineCombined sources
Modified residuei359 – 3591PhosphoserineCombined sources
Modified residuei365 – 3651N6-acetyllysineBy similarity
Modified residuei406 – 4061PhosphoserineCombined sources
Modified residuei409 – 4091PhosphoserineCombined sources
Modified residuei412 – 4121PhosphothreonineCombined sources
Modified residuei418 – 4181PhosphoserineCombined sources
Modified residuei422 – 4221Phosphoserine; by RPS6KA1 and RPS6KB1Combined sources2 Publications
Modified residuei425 – 4251PhosphoserineCombined sources
Modified residuei445 – 4451PhosphoserineCombined sources
Modified residuei459 – 4591PhosphoserineCombined sources
Modified residuei462 – 4621PhosphoserineCombined sources
Modified residuei497 – 4971PhosphoserineBy similarity
Modified residuei498 – 4981PhosphoserineCombined sources
Modified residuei500 – 5001PhosphothreonineBy similarity
Modified residuei504 – 5041PhosphoserineCombined sources
Modified residuei506 – 5061PhosphothreonineBy similarity
Modified residuei586 – 5861N6-acetyllysineCombined sources
Modified residuei597 – 5971PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP23588.
MaxQBiP23588.
PaxDbiP23588.
PeptideAtlasiP23588.
PRIDEiP23588.

PTM databases

iPTMnetiP23588.
PhosphoSiteiP23588.
SwissPalmiP23588.

Miscellaneous databases

PMAP-CutDBP23588.

Expressioni

Gene expression databases

BgeeiENSG00000063046.
CleanExiHS_EIF4B.
ExpressionAtlasiP23588. baseline and differential.
GenevisibleiP23588. HS.

Organism-specific databases

HPAiHPA039072.
HPA046164.

Interactioni

Subunit structurei

Self-associates and interacts with EIF3 p170 subunit.

Binary interactionsi

WithEntry#Exp.IntActNotes
PLK1P533503EBI-970310,EBI-476768
TINF2Q9BSI42EBI-970310,EBI-717399

Protein-protein interaction databases

BioGridi108291. 87 interactions.
IntActiP23588. 36 interactions.
MINTiMINT-210187.
STRINGi9606.ENSP00000262056.

Structurei

Secondary structure

1
611
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi92 – 943Combined sources
Beta strandi96 – 1027Combined sources
Helixi109 – 1157Combined sources
Turni116 – 1183Combined sources
Beta strandi121 – 1255Combined sources
Turni130 – 1334Combined sources
Beta strandi140 – 1467Combined sources
Helixi147 – 1548Combined sources
Helixi155 – 1573Combined sources
Beta strandi167 – 1704Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI8NMR-A88-178[»]
2J76NMR-E77-176[»]
ProteinModelPortaliP23588.
SMRiP23588. Positions 96-176.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23588.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini96 – 17378RRMPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi164 – 331168Arg-richAdd
BLAST
Compositional biasi169 – 325157Asp-richAdd
BLAST

Sequence similaritiesi

Contains 1 RRM (RNA recognition motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
ENOG4111NS2. LUCA.
GeneTreeiENSGT00530000063406.
HOGENOMiHOG000006553.
HOVERGENiHBG006129.
InParanoidiP23588.
KOiK03258.
PhylomeDBiP23588.
TreeFamiTF101525.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033107. EIF4B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23236:SF2. PTHR23236:SF2. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23588-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAASAKKKNK KGKTISLTDF LAEDGGTGGG STYVSKPVSW ADETDDLEGD
60 70 80 90 100
VSTTWHSNDD DVYRAPPIDR SILPTAPRAA REPNIDRSRL PKSPPYTAFL
110 120 130 140 150
GNLPYDVTEE SIKEFFRGLN ISAVRLPREP SNPERLKGFG YAEFEDLDSL
160 170 180 190 200
LSALSLNEES LGNRRIRVDV ADQAQDKDRD DRSFGRDRNR DSDKTDTDWR
210 220 230 240 250
ARPATDSFDD YPPRRGDDSF GDKYRDRYDS DRYRDGYRDG YRDGPRRDMD
260 270 280 290 300
RYGGRDRYDD RGSRDYDRGY DSRIGSGRRA FGSGYRRDDD YRGGGDRYED
310 320 330 340 350
RYDRRDDRSW SSRDDYSRDD YRRDDRGPPQ RPKLNLKPRS TPKEDDSSAS
360 370 380 390 400
TSQSTRAASI FGGAKPVDTA AREREVEERL QKEQEKLQRQ LDEPKLERRP
410 420 430 440 450
RERHPSWRSE ETQERERSRT GSESSQTGTS TTSSRNARRR ESEKSLENET
460 470 480 490 500
LNKEEDCHSP TSKPPKPDQP LKVMPAPPPK ENAWVKRSSN PPARSQSSDT
510 520 530 540 550
EQQSPTSGGG KVAPAQPSEE GPGRKDENKV DGMNAPKGQT GNSSRGPGDG
560 570 580 590 600
GNRDHWKESD RKDGKKDQDS RSAPEPKKPE ENPASKFSSA SKYAALSVDG
610
EDENEGEDYA E
Length:611
Mass (Da):69,151
Last modified:September 2, 2008 - v2
Checksum:i31CEFEA865FB10D2
GO
Isoform 2 (identifier: P23588-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     121-159: Missing.

Note: No experimental confirmation available.
Show »
Length:572
Mass (Da):64,805
Checksum:i960DF53E27A1C2AA
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti164 – 1641R → K in BAD96248 (Ref. 5) Curated
Sequence conflicti246 – 2461R → C in AAH98437 (PubMed:15489334).Curated
Sequence conflicti343 – 3431K → E in CAA39265 (PubMed:2390971).Curated
Sequence conflicti391 – 3922LD → WN in CAA39265 (PubMed:2390971).Curated
Sequence conflicti471 – 4711L → Q in AAH73154 (PubMed:15489334).Curated
Sequence conflicti486 – 4861K → R in BAD96248 (Ref. 5) Curated
Sequence conflicti611 – 6111E → D in CAG33239 (Ref. 4) Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti203 – 2031P → R Found in a renal cell carcinoma case; somatic mutation. 1 Publication
VAR_064710

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei121 – 15939Missing in isoform 2. 1 PublicationVSP_057351Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55733 Genomic DNA. Translation: CAA39265.1.
AB076839 mRNA. Translation: BAB82380.1.
AK299526 mRNA. Translation: BAG61475.1.
CR456958 mRNA. Translation: CAG33239.1.
AK222528 mRNA. Translation: BAD96248.1.
AC068888 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96657.1.
BC073139 mRNA. Translation: AAH73139.1.
BC073154 mRNA. Translation: AAH73154.1.
BC098437 mRNA. Translation: AAH98437.1.
CCDSiCCDS41788.1. [P23588-1]
PIRiS12566.
RefSeqiNP_001408.2. NM_001417.5. [P23588-1]
UniGeneiHs.648394.
Hs.702041.

Genome annotation databases

EnsembliENST00000262056; ENSP00000262056; ENSG00000063046. [P23588-1]
ENST00000416762; ENSP00000412530; ENSG00000063046. [P23588-2]
GeneIDi1975.
KEGGihsa:1975.
UCSCiuc001sbh.5. human. [P23588-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55733 Genomic DNA. Translation: CAA39265.1.
AB076839 mRNA. Translation: BAB82380.1.
AK299526 mRNA. Translation: BAG61475.1.
CR456958 mRNA. Translation: CAG33239.1.
AK222528 mRNA. Translation: BAD96248.1.
AC068888 Genomic DNA. No translation available.
CH471054 Genomic DNA. Translation: EAW96657.1.
BC073139 mRNA. Translation: AAH73139.1.
BC073154 mRNA. Translation: AAH73154.1.
BC098437 mRNA. Translation: AAH98437.1.
CCDSiCCDS41788.1. [P23588-1]
PIRiS12566.
RefSeqiNP_001408.2. NM_001417.5. [P23588-1]
UniGeneiHs.648394.
Hs.702041.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WI8NMR-A88-178[»]
2J76NMR-E77-176[»]
ProteinModelPortaliP23588.
SMRiP23588. Positions 96-176.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108291. 87 interactions.
IntActiP23588. 36 interactions.
MINTiMINT-210187.
STRINGi9606.ENSP00000262056.

PTM databases

iPTMnetiP23588.
PhosphoSiteiP23588.
SwissPalmiP23588.

Polymorphism and mutation databases

BioMutaiEIF4B.
DMDMi205371761.

Proteomic databases

EPDiP23588.
MaxQBiP23588.
PaxDbiP23588.
PeptideAtlasiP23588.
PRIDEiP23588.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000262056; ENSP00000262056; ENSG00000063046. [P23588-1]
ENST00000416762; ENSP00000412530; ENSG00000063046. [P23588-2]
GeneIDi1975.
KEGGihsa:1975.
UCSCiuc001sbh.5. human. [P23588-1]

Organism-specific databases

CTDi1975.
GeneCardsiEIF4B.
HGNCiHGNC:3285. EIF4B.
HPAiHPA039072.
HPA046164.
MIMi603928. gene.
neXtProtiNX_P23588.
PharmGKBiPA27713.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0118. Eukaryota.
ENOG4111NS2. LUCA.
GeneTreeiENSGT00530000063406.
HOGENOMiHOG000006553.
HOVERGENiHBG006129.
InParanoidiP23588.
KOiK03258.
PhylomeDBiP23588.
TreeFamiTF101525.

Enzyme and pathway databases

ReactomeiR-HSA-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-HSA-166208. mTORC1-mediated signalling.
R-HSA-429947. Deadenylation of mRNA.
R-HSA-72649. Translation initiation complex formation.
R-HSA-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-HSA-72702. Ribosomal scanning and start codon recognition.
R-HSA-72706. GTP hydrolysis and joining of the 60S ribosomal subunit.
SIGNORiP23588.

Miscellaneous databases

ChiTaRSiEIF4B. human.
EvolutionaryTraceiP23588.
GeneWikiiEIF4B.
GenomeRNAii1975.
PMAP-CutDBP23588.
PROiP23588.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000063046.
CleanExiHS_EIF4B.
ExpressionAtlasiP23588. baseline and differential.
GenevisibleiP23588. HS.

Family and domain databases

Gene3Di3.30.70.330. 1 hit.
InterProiIPR033107. EIF4B.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23236:SF2. PTHR23236:SF2. 1 hit.
PfamiPF00076. RRM_1. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 1 hit.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 1 hit.
PROSITEiPS50102. RRM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF4B_HUMAN
AccessioniPrimary (citable) accession number: P23588
Secondary accession number(s): B4DS13
, Q4G0E3, Q53HQ2, Q6GPH5, Q6IB46, Q8WYK5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: September 2, 2008
Last modified: September 7, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. Translation initiation factors
    List of translation initiation factor entries
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.