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P23585

- HXT2_YEAST

UniProt

P23585 - HXT2_YEAST

Protein

High-affinity glucose transporter HXT2

Gene

HXT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    High-affinity glucose transporter. Is only indispensable for growth on low glucose-containing media, because S.cerevisiae possesses other sugar transporters.

    GO - Molecular functioni

    1. fructose transmembrane transporter activity Source: SGD
    2. glucose transmembrane transporter activity Source: SGD
    3. mannose transmembrane transporter activity Source: SGD
    4. pentose transmembrane transporter activity Source: SGD

    GO - Biological processi

    1. fructose transport Source: GOC
    2. glucose transport Source: SGD
    3. mannose transport Source: GOC
    4. pentose transport Source: GOC

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32719-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.1.111. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High-affinity glucose transporter HXT2
    Gene namesi
    Name:HXT2
    Ordered Locus Names:YMR011W
    ORF Names:YM8270.15
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIII

    Organism-specific databases

    CYGDiYMR011w.
    SGDiS000004613. HXT2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: SGD

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 541540High-affinity glucose transporter HXT2PRO_0000050392Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei11 – 111Phosphoserine1 Publication
    Modified residuei13 – 131Phosphoserine1 Publication
    Modified residuei17 – 171Phosphoserine2 Publications
    Modified residuei20 – 201Phosphoserine2 Publications
    Modified residuei29 – 291Phosphothreonine3 Publications
    Modified residuei32 – 321Phosphoserine2 Publications
    Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
    Modified residuei266 – 2661Phosphoserine; by PKASequence Analysis
    Modified residuei539 – 5391Phosphoserine; by PKASequence Analysis

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP23585.
    PaxDbiP23585.
    PeptideAtlasiP23585.

    Expressioni

    Inductioni

    Repressed at high glucose concentrations.

    Gene expression databases

    GenevestigatoriP23585.

    Interactioni

    Protein-protein interaction databases

    BioGridi35180. 96 interactions.
    DIPiDIP-7912N.
    IntActiP23585. 6 interactions.
    MINTiMINT-4497394.
    STRINGi4932.YMR011W.

    Structurei

    3D structure databases

    ProteinModelPortaliP23585.
    SMRiP23585. Positions 56-512.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 4948CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini71 – 10737ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini129 – 1346CytoplasmicSequence Analysis
    Topological domaini156 – 1627ExtracellularSequence Analysis
    Topological domaini184 – 1929CytoplasmicSequence Analysis
    Topological domaini214 – 22714ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini249 – 32779CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini348 – 3503ExtracellularSequence Analysis
    Topological domaini372 – 3798CytoplasmicSequence Analysis
    Topological domaini401 – 41818ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini440 – 45617CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini478 – 4847ExtracellularSequence Analysis
    Topological domaini506 – 54136CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei50 – 7021Helical; Name=1Sequence AnalysisAdd
    BLAST
    Transmembranei108 – 12821Helical; Name=2Sequence AnalysisAdd
    BLAST
    Transmembranei135 – 15521Helical; Name=3Sequence AnalysisAdd
    BLAST
    Transmembranei163 – 18321Helical; Name=4Sequence AnalysisAdd
    BLAST
    Transmembranei193 – 21321Helical; Name=5Sequence AnalysisAdd
    BLAST
    Transmembranei228 – 24821Helical; Name=6Sequence AnalysisAdd
    BLAST
    Transmembranei328 – 34720Helical; Name=7Sequence AnalysisAdd
    BLAST
    Transmembranei351 – 37121Helical; Name=8Sequence AnalysisAdd
    BLAST
    Transmembranei380 – 40021Helical; Name=9Sequence AnalysisAdd
    BLAST
    Transmembranei419 – 43921Helical; Name=10Sequence AnalysisAdd
    BLAST
    Transmembranei457 – 47721Helical; Name=11Sequence AnalysisAdd
    BLAST
    Transmembranei485 – 50521Helical; Name=12Sequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00720000108754.
    HOGENOMiHOG000202870.
    KOiK08139.
    OMAiCAFGGLT.
    OrthoDBiEOG73JM4H.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    PRINTSiPR00171. SUGRTRNSPORT.
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00879. SP. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23585-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSEFATSRVE SGSQQTSIHS TPIVQKLETD ESPIQTKSEY TNAELPAKPI    50
    AAYWTVICLC LMIAFGGFVF GWDTGTISGF VNQTDFKRRF GQMKSDGTYY 100
    LSDVRTGLIV GIFNIGCAFG GLTLGRLGDM YGRRIGLMCV VLVYIVGIVI 150
    QIASSDKWYQ YFIGRIISGM GVGGIAVLSP TLISETAPKH IRGTCVSFYQ 200
    LMITLGIFLG YCTNYGTKDY SNSVQWRVPL GLNFAFAIFM IAGMLMVPES 250
    PRFLVEKGRY EDAKRSLAKS NKVTIEDPSI VAEMDTIMAN VETERLAGNA 300
    SWGELFSNKG AILPRVIMGI MIQSLQQLTG NNYFFYYGTT IFNAVGMKDS 350
    FQTSIVLGIV NFASTFVALY TVDKFGRRKC LLGGSASMAI CFVIFSTVGV 400
    TSLYPNGKDQ PSSKAAGNVM IVFTCLFIFF FAISWAPIAY VIVAESYPLR 450
    VKNRAMAIAV GANWIWGFLI GFFTPFITSA IGFSYGYVFM GCLVFSFFYV 500
    FFFVCETKGL TLEEVNEMYV EGVKPWKSGS WISKEKRVSE E 541
    Length:541
    Mass (Da):59,841
    Last modified:November 1, 1991 - v1
    Checksum:i6AEFEC0A87391CA7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33270 Genomic DNA. Translation: AAA34701.1.
    Z48613 Genomic DNA. Translation: CAA88528.1.
    BK006946 Genomic DNA. Translation: DAA09909.1.
    PIRiA36380. MMBYH2.
    RefSeqiNP_013724.1. NM_001182507.1.

    Genome annotation databases

    EnsemblFungiiYMR011W; YMR011W; YMR011W.
    GeneIDi855023.
    KEGGisce:YMR011W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M33270 Genomic DNA. Translation: AAA34701.1 .
    Z48613 Genomic DNA. Translation: CAA88528.1 .
    BK006946 Genomic DNA. Translation: DAA09909.1 .
    PIRi A36380. MMBYH2.
    RefSeqi NP_013724.1. NM_001182507.1.

    3D structure databases

    ProteinModelPortali P23585.
    SMRi P23585. Positions 56-512.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35180. 96 interactions.
    DIPi DIP-7912N.
    IntActi P23585. 6 interactions.
    MINTi MINT-4497394.
    STRINGi 4932.YMR011W.

    Protein family/group databases

    TCDBi 2.A.1.1.111. the major facilitator superfamily (mfs).

    Proteomic databases

    MaxQBi P23585.
    PaxDbi P23585.
    PeptideAtlasi P23585.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YMR011W ; YMR011W ; YMR011W .
    GeneIDi 855023.
    KEGGi sce:YMR011W.

    Organism-specific databases

    CYGDi YMR011w.
    SGDi S000004613. HXT2.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00720000108754.
    HOGENOMi HOG000202870.
    KOi K08139.
    OMAi CAFGGLT.
    OrthoDBi EOG73JM4H.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32719-MONOMER.

    Miscellaneous databases

    NextBioi 978213.

    Gene expression databases

    Genevestigatori P23585.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR005828. Sub_transporter.
    IPR003663. Sugar/inositol_transpt.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    PRINTSi PR00171. SUGRTRNSPORT.
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00879. SP. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 1 hit.
    PS00217. SUGAR_TRANSPORT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport."
      Kruckeberg A.L., Bisson L.F.
      Mol. Cell. Biol. 10:5903-5913(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
      Kim H., Melen K., Oesterberg M., von Heijne G.
      Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: ATCC 208353 / W303-1A.
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; SER-13; SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiHXT2_YEAST
    AccessioniPrimary (citable) accession number: P23585
    Secondary accession number(s): D6VZI5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome XIII
      Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

    External Data

    Dasty 3