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P23585

- HXT2_YEAST

UniProt

P23585 - HXT2_YEAST

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Protein

High-affinity glucose transporter HXT2

Gene

HXT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

High-affinity glucose transporter. Is only indispensable for growth on low glucose-containing media, because S.cerevisiae possesses other sugar transporters.

GO - Molecular functioni

  1. fructose transmembrane transporter activity Source: SGD
  2. glucose transmembrane transporter activity Source: SGD
  3. mannose transmembrane transporter activity Source: SGD
  4. pentose transmembrane transporter activity Source: SGD

GO - Biological processi

  1. fructose transport Source: GOC
  2. glucose transport Source: SGD
  3. mannose transport Source: GOC
  4. pentose transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32719-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.111. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glucose transporter HXT2
Gene namesi
Name:HXT2
Ordered Locus Names:YMR011W
ORF Names:YM8270.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR011w.
SGDiS000004613. HXT2.

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 541540High-affinity glucose transporter HXT2PRO_0000050392Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei11 – 111Phosphoserine1 Publication
Modified residuei13 – 131Phosphoserine1 Publication
Modified residuei17 – 171Phosphoserine2 Publications
Modified residuei20 – 201Phosphoserine2 Publications
Modified residuei29 – 291Phosphothreonine3 Publications
Modified residuei32 – 321Phosphoserine2 Publications
Glycosylationi82 – 821N-linked (GlcNAc...)Sequence Analysis
Modified residuei266 – 2661Phosphoserine; by PKASequence Analysis
Modified residuei539 – 5391Phosphoserine; by PKASequence Analysis

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP23585.
PaxDbiP23585.
PeptideAtlasiP23585.

Expressioni

Inductioni

Repressed at high glucose concentrations.

Gene expression databases

GenevestigatoriP23585.

Interactioni

Protein-protein interaction databases

BioGridi35180. 96 interactions.
DIPiDIP-7912N.
IntActiP23585. 6 interactions.
MINTiMINT-4497394.
STRINGi4932.YMR011W.

Structurei

3D structure databases

ProteinModelPortaliP23585.
SMRiP23585. Positions 56-512.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 4948CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini71 – 10737ExtracellularSequence AnalysisAdd
BLAST
Topological domaini129 – 1346CytoplasmicSequence Analysis
Topological domaini156 – 1627ExtracellularSequence Analysis
Topological domaini184 – 1929CytoplasmicSequence Analysis
Topological domaini214 – 22714ExtracellularSequence AnalysisAdd
BLAST
Topological domaini249 – 32779CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini348 – 3503ExtracellularSequence Analysis
Topological domaini372 – 3798CytoplasmicSequence Analysis
Topological domaini401 – 41818ExtracellularSequence AnalysisAdd
BLAST
Topological domaini440 – 45617CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini478 – 4847ExtracellularSequence Analysis
Topological domaini506 – 54136CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei50 – 7021Helical; Name=1Sequence AnalysisAdd
BLAST
Transmembranei108 – 12821Helical; Name=2Sequence AnalysisAdd
BLAST
Transmembranei135 – 15521Helical; Name=3Sequence AnalysisAdd
BLAST
Transmembranei163 – 18321Helical; Name=4Sequence AnalysisAdd
BLAST
Transmembranei193 – 21321Helical; Name=5Sequence AnalysisAdd
BLAST
Transmembranei228 – 24821Helical; Name=6Sequence AnalysisAdd
BLAST
Transmembranei328 – 34720Helical; Name=7Sequence AnalysisAdd
BLAST
Transmembranei351 – 37121Helical; Name=8Sequence AnalysisAdd
BLAST
Transmembranei380 – 40021Helical; Name=9Sequence AnalysisAdd
BLAST
Transmembranei419 – 43921Helical; Name=10Sequence AnalysisAdd
BLAST
Transmembranei457 – 47721Helical; Name=11Sequence AnalysisAdd
BLAST
Transmembranei485 – 50521Helical; Name=12Sequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
InParanoidiP23585.
KOiK08139.
OMAiCAFGGLT.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23585-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSEFATSRVE SGSQQTSIHS TPIVQKLETD ESPIQTKSEY TNAELPAKPI
60 70 80 90 100
AAYWTVICLC LMIAFGGFVF GWDTGTISGF VNQTDFKRRF GQMKSDGTYY
110 120 130 140 150
LSDVRTGLIV GIFNIGCAFG GLTLGRLGDM YGRRIGLMCV VLVYIVGIVI
160 170 180 190 200
QIASSDKWYQ YFIGRIISGM GVGGIAVLSP TLISETAPKH IRGTCVSFYQ
210 220 230 240 250
LMITLGIFLG YCTNYGTKDY SNSVQWRVPL GLNFAFAIFM IAGMLMVPES
260 270 280 290 300
PRFLVEKGRY EDAKRSLAKS NKVTIEDPSI VAEMDTIMAN VETERLAGNA
310 320 330 340 350
SWGELFSNKG AILPRVIMGI MIQSLQQLTG NNYFFYYGTT IFNAVGMKDS
360 370 380 390 400
FQTSIVLGIV NFASTFVALY TVDKFGRRKC LLGGSASMAI CFVIFSTVGV
410 420 430 440 450
TSLYPNGKDQ PSSKAAGNVM IVFTCLFIFF FAISWAPIAY VIVAESYPLR
460 470 480 490 500
VKNRAMAIAV GANWIWGFLI GFFTPFITSA IGFSYGYVFM GCLVFSFFYV
510 520 530 540
FFFVCETKGL TLEEVNEMYV EGVKPWKSGS WISKEKRVSE E
Length:541
Mass (Da):59,841
Last modified:November 1, 1991 - v1
Checksum:i6AEFEC0A87391CA7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33270 Genomic DNA. Translation: AAA34701.1.
Z48613 Genomic DNA. Translation: CAA88528.1.
BK006946 Genomic DNA. Translation: DAA09909.1.
PIRiA36380. MMBYH2.
RefSeqiNP_013724.1. NM_001182507.1.

Genome annotation databases

EnsemblFungiiYMR011W; YMR011W; YMR011W.
GeneIDi855023.
KEGGisce:YMR011W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33270 Genomic DNA. Translation: AAA34701.1 .
Z48613 Genomic DNA. Translation: CAA88528.1 .
BK006946 Genomic DNA. Translation: DAA09909.1 .
PIRi A36380. MMBYH2.
RefSeqi NP_013724.1. NM_001182507.1.

3D structure databases

ProteinModelPortali P23585.
SMRi P23585. Positions 56-512.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35180. 96 interactions.
DIPi DIP-7912N.
IntActi P23585. 6 interactions.
MINTi MINT-4497394.
STRINGi 4932.YMR011W.

Protein family/group databases

TCDBi 2.A.1.1.111. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBi P23585.
PaxDbi P23585.
PeptideAtlasi P23585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR011W ; YMR011W ; YMR011W .
GeneIDi 855023.
KEGGi sce:YMR011W.

Organism-specific databases

CYGDi YMR011w.
SGDi S000004613. HXT2.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00720000108754.
HOGENOMi HOG000202870.
InParanoidi P23585.
KOi K08139.
OMAi CAFGGLT.
OrthoDBi EOG73JM4H.

Enzyme and pathway databases

BioCyci YEAST:G3O-32719-MONOMER.

Miscellaneous databases

NextBioi 978213.

Gene expression databases

Genevestigatori P23585.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
PRINTSi PR00171. SUGRTRNSPORT.
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00879. SP. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport."
    Kruckeberg A.L., Bisson L.F.
    Mol. Cell. Biol. 10:5903-5913(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; SER-13; SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHXT2_YEAST
AccessioniPrimary (citable) accession number: P23585
Secondary accession number(s): D6VZI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3