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P23585

- HXT2_YEAST

UniProt

P23585 - HXT2_YEAST

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Protein
High-affinity glucose transporter HXT2
Gene
HXT2, YMR011W, YM8270.15
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

High-affinity glucose transporter. Is only indispensable for growth on low glucose-containing media, because S.cerevisiae possesses other sugar transporters.

GO - Molecular functioni

  1. fructose transmembrane transporter activity Source: SGD
  2. glucose transmembrane transporter activity Source: SGD
  3. mannose transmembrane transporter activity Source: SGD
  4. pentose transmembrane transporter activity Source: SGD
Complete GO annotation...

GO - Biological processi

  1. fructose transport Source: GOC
  2. glucose transport Source: SGD
  3. mannose transport Source: GOC
  4. pentose transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-32719-MONOMER.

Protein family/group databases

TCDBi2.A.1.1.111. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
High-affinity glucose transporter HXT2
Gene namesi
Name:HXT2
Ordered Locus Names:YMR011W
ORF Names:YM8270.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIII

Organism-specific databases

CYGDiYMR011w.
SGDiS000004613. HXT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 4948Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei50 – 7021Helical; Name=1; Reviewed prediction
Add
BLAST
Topological domaini71 – 10737Extracellular Reviewed prediction
Add
BLAST
Transmembranei108 – 12821Helical; Name=2; Reviewed prediction
Add
BLAST
Topological domaini129 – 1346Cytoplasmic Reviewed prediction
Transmembranei135 – 15521Helical; Name=3; Reviewed prediction
Add
BLAST
Topological domaini156 – 1627Extracellular Reviewed prediction
Transmembranei163 – 18321Helical; Name=4; Reviewed prediction
Add
BLAST
Topological domaini184 – 1929Cytoplasmic Reviewed prediction
Transmembranei193 – 21321Helical; Name=5; Reviewed prediction
Add
BLAST
Topological domaini214 – 22714Extracellular Reviewed prediction
Add
BLAST
Transmembranei228 – 24821Helical; Name=6; Reviewed prediction
Add
BLAST
Topological domaini249 – 32779Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei328 – 34720Helical; Name=7; Reviewed prediction
Add
BLAST
Topological domaini348 – 3503Extracellular Reviewed prediction
Transmembranei351 – 37121Helical; Name=8; Reviewed prediction
Add
BLAST
Topological domaini372 – 3798Cytoplasmic Reviewed prediction
Transmembranei380 – 40021Helical; Name=9; Reviewed prediction
Add
BLAST
Topological domaini401 – 41818Extracellular Reviewed prediction
Add
BLAST
Transmembranei419 – 43921Helical; Name=10; Reviewed prediction
Add
BLAST
Topological domaini440 – 45617Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei457 – 47721Helical; Name=11; Reviewed prediction
Add
BLAST
Topological domaini478 – 4847Extracellular Reviewed prediction
Transmembranei485 – 50521Helical; Name=12; Reviewed prediction
Add
BLAST
Topological domaini506 – 54136Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 541540High-affinity glucose transporter HXT2
PRO_0000050392Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei11 – 111Phosphoserine1 Publication
Modified residuei13 – 131Phosphoserine1 Publication
Modified residuei17 – 171Phosphoserine2 Publications
Modified residuei20 – 201Phosphoserine2 Publications
Modified residuei29 – 291Phosphothreonine3 Publications
Modified residuei32 – 321Phosphoserine2 Publications
Glycosylationi82 – 821N-linked (GlcNAc...) Reviewed prediction
Modified residuei266 – 2661Phosphoserine; by PKA Reviewed prediction
Modified residuei539 – 5391Phosphoserine; by PKA Reviewed prediction

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP23585.
PaxDbiP23585.
PeptideAtlasiP23585.

Expressioni

Inductioni

Repressed at high glucose concentrations.

Gene expression databases

GenevestigatoriP23585.

Interactioni

Protein-protein interaction databases

BioGridi35180. 96 interactions.
DIPiDIP-7912N.
IntActiP23585. 6 interactions.
MINTiMINT-4497394.
STRINGi4932.YMR011W.

Structurei

3D structure databases

ProteinModelPortaliP23585.
SMRiP23585. Positions 56-512.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0477.
GeneTreeiENSGT00720000108754.
HOGENOMiHOG000202870.
KOiK08139.
OMAiCAFGGLT.
OrthoDBiEOG73JM4H.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
PRINTSiPR00171. SUGRTRNSPORT.
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00879. SP. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23585-1 [UniParc]FASTAAdd to Basket

« Hide

MSEFATSRVE SGSQQTSIHS TPIVQKLETD ESPIQTKSEY TNAELPAKPI    50
AAYWTVICLC LMIAFGGFVF GWDTGTISGF VNQTDFKRRF GQMKSDGTYY 100
LSDVRTGLIV GIFNIGCAFG GLTLGRLGDM YGRRIGLMCV VLVYIVGIVI 150
QIASSDKWYQ YFIGRIISGM GVGGIAVLSP TLISETAPKH IRGTCVSFYQ 200
LMITLGIFLG YCTNYGTKDY SNSVQWRVPL GLNFAFAIFM IAGMLMVPES 250
PRFLVEKGRY EDAKRSLAKS NKVTIEDPSI VAEMDTIMAN VETERLAGNA 300
SWGELFSNKG AILPRVIMGI MIQSLQQLTG NNYFFYYGTT IFNAVGMKDS 350
FQTSIVLGIV NFASTFVALY TVDKFGRRKC LLGGSASMAI CFVIFSTVGV 400
TSLYPNGKDQ PSSKAAGNVM IVFTCLFIFF FAISWAPIAY VIVAESYPLR 450
VKNRAMAIAV GANWIWGFLI GFFTPFITSA IGFSYGYVFM GCLVFSFFYV 500
FFFVCETKGL TLEEVNEMYV EGVKPWKSGS WISKEKRVSE E 541
Length:541
Mass (Da):59,841
Last modified:November 1, 1991 - v1
Checksum:i6AEFEC0A87391CA7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33270 Genomic DNA. Translation: AAA34701.1.
Z48613 Genomic DNA. Translation: CAA88528.1.
BK006946 Genomic DNA. Translation: DAA09909.1.
PIRiA36380. MMBYH2.
RefSeqiNP_013724.1. NM_001182507.1.

Genome annotation databases

EnsemblFungiiYMR011W; YMR011W; YMR011W.
GeneIDi855023.
KEGGisce:YMR011W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M33270 Genomic DNA. Translation: AAA34701.1 .
Z48613 Genomic DNA. Translation: CAA88528.1 .
BK006946 Genomic DNA. Translation: DAA09909.1 .
PIRi A36380. MMBYH2.
RefSeqi NP_013724.1. NM_001182507.1.

3D structure databases

ProteinModelPortali P23585.
SMRi P23585. Positions 56-512.
ModBasei Search...

Protein-protein interaction databases

BioGridi 35180. 96 interactions.
DIPi DIP-7912N.
IntActi P23585. 6 interactions.
MINTi MINT-4497394.
STRINGi 4932.YMR011W.

Protein family/group databases

TCDBi 2.A.1.1.111. the major facilitator superfamily (mfs).

Proteomic databases

MaxQBi P23585.
PaxDbi P23585.
PeptideAtlasi P23585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YMR011W ; YMR011W ; YMR011W .
GeneIDi 855023.
KEGGi sce:YMR011W.

Organism-specific databases

CYGDi YMR011w.
SGDi S000004613. HXT2.

Phylogenomic databases

eggNOGi COG0477.
GeneTreei ENSGT00720000108754.
HOGENOMi HOG000202870.
KOi K08139.
OMAi CAFGGLT.
OrthoDBi EOG73JM4H.

Enzyme and pathway databases

BioCyci YEAST:G3O-32719-MONOMER.

Miscellaneous databases

NextBioi 978213.

Gene expression databases

Genevestigatori P23585.

Family and domain databases

InterProi IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR005828. Sub_transporter.
IPR003663. Sugar/inositol_transpt.
IPR005829. Sugar_transporter_CS.
[Graphical view ]
Pfami PF00083. Sugar_tr. 1 hit.
[Graphical view ]
PRINTSi PR00171. SUGRTRNSPORT.
SUPFAMi SSF103473. SSF103473. 1 hit.
TIGRFAMsi TIGR00879. SP. 1 hit.
PROSITEi PS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
PS00217. SUGAR_TRANSPORT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The HXT2 gene of Saccharomyces cerevisiae is required for high-affinity glucose transport."
    Kruckeberg A.L., Bisson L.F.
    Mol. Cell. Biol. 10:5903-5913(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-11; SER-13; SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-29, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17; SER-20; THR-29 AND SER-32, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiHXT2_YEAST
AccessioniPrimary (citable) accession number: P23585
Secondary accession number(s): D6VZI5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 14, 2014
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Glucose transport is thought to be mediated by two kinetically distinct systems, a glucose-repressible high-affinity system and a constitutive low-affinity system.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

External Data

Dasty 3

Similar proteinsi