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Protein

Acrosin

Gene

Acr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891Charge relay systemBy similarity
Active sitei143 – 1431Charge relay systemBy similarity
Active sitei241 – 2411Charge relay systemBy similarity

GO - Molecular functioni

  • amidase activity Source: UniProtKB
  • drug binding Source: UniProtKB
  • fucose binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • acrosome reaction Source: UniProtKB
  • activation of adenylate cyclase activity Source: UniProtKB
  • binding of sperm to zona pellucida Source: MGI
  • penetration of zona pellucida Source: MGI
  • response to steroid hormone Source: Ensembl
  • single fertilization Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiREACT_279120. Acrosome Reaction.

Protein family/group databases

MEROPSiS01.223.

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:Acr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:87884. Acr.

Subcellular locationi

GO - Cellular componenti

  • acrosomal matrix Source: HGNC
  • acrosomal vesicle Source: MGI
  • Golgi-associated vesicle Source: Ensembl
  • nucleus Source: MGI
  • protein complex Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Add
BLAST
Chaini20 – 436417AcrosinPRO_0000027522Add
BLAST
Chaini20 – 4223Acrosin light chainPRO_0000027523Add
BLAST
Chaini43 – 345303Acrosin heavy chainPRO_0000027524Add
BLAST
Propeptidei346 – 43691Pro-richBy similarityPRO_0000027525Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi22 – 221N-linked (GlcNAc...)By similarity
Disulfide bondi25 ↔ 155Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi29 ↔ 163Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
Disulfide bondi178 ↔ 247PROSITE-ProRule annotation
Disulfide bondi210 ↔ 226PROSITE-ProRule annotation
Glycosylationi211 – 2111N-linked (GlcNAc...)By similarity
Disulfide bondi237 ↔ 267PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP23578.
PRIDEiP23578.

Expressioni

Gene expression databases

BgeeiP23578.
CleanExiMM_ACR.
ExpressionAtlasiP23578. baseline.
GenevisibleiP23578. MM.

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi197930. 1 interaction.
STRINGi10090.ENSMUSP00000023295.

Structurei

3D structure databases

ProteinModelPortaliP23578.
SMRiP23578. Positions 43-303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 291249Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00800000124034.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP23578.
OMAiKKVYDWR.
OrthoDBiEOG75B84T.
PhylomeDBiP23578.
TreeFamiTF335943.

Family and domain databases

InterProiIPR012267. Pept_S1A_acrosin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEMLPTVAV LVLAVSVVAK DNTTCDGPCG LRFRQNSQAG TRIVSGQSAQ
60 70 80 90 100
LGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW
110 120 130 140 150
RLVFGAQEIE YGRNKPVKEP QQERYVQKIV IHEKYNVVTE GNDIALLKIT
160 170 180 190 200
PPVTCGNFIG PCCLPHFKAG PPQIPHTCYV TGWGYIKEKA PRPSPVLMEA
210 220 230 240 250
RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDN
260 270 280 290 300
VDSPFVVVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPNALHLIQP
310 320 330 340 350
ATPHPPTTRH PMVSFHPPSL RPPWYFQHLP SRPLYLRPLR PLLHRPSSTQ
360 370 380 390 400
TSSSLMPLLS PPTPAQPASF TIATQHMRHR TTLSFARRLQ RLIEALKMRT
410 420 430
YPMKHPSQYS GPRNYHYRFS TFEPLSNKPS EPFLHS
Length:436
Mass (Da):48,929
Last modified:November 1, 1991 - v1
Checksum:i4CC6EAD56CB51F54
GO

Sequence cautioni

The sequence AAA40124.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence BAA00651.1 differs from that shown. Reason: Erroneous initiation. Curated
The sequence CAA36704.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81Missing in CAA36704 (PubMed:2111255).Curated
Sequence conflicti8 – 81Missing in AAA40124 (PubMed:2111255).Curated
Sequence conflicti10 – 145VLVLA → FWSVK in CAA36704 (PubMed:2111255).Curated
Sequence conflicti10 – 145VLVLA → FWSVK in AAA40124 (PubMed:2111255).Curated
Sequence conflicti17 – 182VV → AG in CAA36704 (PubMed:2111255).Curated
Sequence conflicti17 – 182VV → AG in AAA40124 (PubMed:2111255).Curated
Sequence conflicti23 – 231T → A in CAA36704 (PubMed:2111255).Curated
Sequence conflicti23 – 231T → A in AAA40124 (PubMed:2111255).Curated
Sequence conflicti26 – 261D → F in CAA36704 (PubMed:2111255).Curated
Sequence conflicti26 – 261D → F in AAA40124 (PubMed:2111255).Curated
Sequence conflicti33 – 331F → T in CAA36704 (PubMed:2111255).Curated
Sequence conflicti33 – 331F → T in AAA40124 (PubMed:2111255).Curated
Sequence conflicti50 – 512QL → HV in CAA36704 (PubMed:2111255).Curated
Sequence conflicti50 – 512QL → HV in AAA40124 (PubMed:2111255).Curated
Sequence conflicti122 – 1221Q → E in CAA36704 (PubMed:2111255).Curated
Sequence conflicti122 – 1221Q → E in AAA40124 (PubMed:2111255).Curated
Sequence conflicti122 – 1221Q → E (PubMed:1783391).Curated
Sequence conflicti149 – 1491I → V in CAA36704 (PubMed:2111255).Curated
Sequence conflicti149 – 1491I → V in AAA40124 (PubMed:2111255).Curated
Sequence conflicti149 – 1491I → V (PubMed:1783391).Curated
Sequence conflicti173 – 1742QI → RNT in AAA40124 (PubMed:2111255).Curated
Sequence conflicti173 – 1731Q → RK in CAA36704 (PubMed:2111255).Curated
Sequence conflicti177 – 1837TCYVTGW → LLRDWV in AAA40124 (PubMed:2111255).Curated
Sequence conflicti185 – 1862YI → IH in AAA40124 (PubMed:2111255).Curated
Sequence conflicti188 – 1892EK → RE in CAA36704 (PubMed:2111255).Curated
Sequence conflicti188 – 1892EK → RE in AAA40124 (PubMed:2111255).Curated
Sequence conflicti251 – 2533VDS → ARQ in CAA36704 (PubMed:2111255).Curated
Sequence conflicti251 – 2533VDS → ARQ in AAA40124 (PubMed:2111255).Curated
Sequence conflicti255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA40124 (PubMed:2111255).CuratedAdd
BLAST
Sequence conflicti255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA37163 (PubMed:1783391).CuratedAdd
BLAST
Sequence conflicti299 – 3002QP → PA in CAA36704 (PubMed:2111255).Curated
Sequence conflicti299 – 3002QP → PA in AAA40124 (PubMed:2111255).Curated
Sequence conflicti320 – 3201L → F in CAA36704 (PubMed:2111255).Curated
Sequence conflicti320 – 3201L → F in AAA40124 (PubMed:2111255).Curated
Sequence conflicti405 – 4051H → Y in CAA36704 (PubMed:2111255).Curated
Sequence conflicti405 – 4051H → Y in AAA40124 (PubMed:2111255).Curated
Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in CAA36704 (PubMed:2111255).CuratedAdd
BLAST
Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in AAA40124 (PubMed:2111255).CuratedAdd
BLAST
Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV (PubMed:1783391).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00754 mRNA. Translation: BAA00651.1. Different initiation.
S66245, S64500, S66243 Genomic DNA. Translation: AAB20293.1.
X52466 mRNA. Translation: CAA36704.1. Different initiation.
M85170 mRNA. Translation: AAA40124.1. Different initiation.
M96430
, M96426, M96427, M96428 Genomic DNA. Translation: AAA37163.1.
CCDSiCCDS27755.1.
PIRiA37344.
JX0172.
RefSeqiNP_038483.1. NM_013455.3.
UniGeneiMm.57.

Genome annotation databases

EnsembliENSMUST00000023295; ENSMUSP00000023295; ENSMUSG00000022622.
GeneIDi11434.
UCSCiuc007xhc.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00754 mRNA. Translation: BAA00651.1. Different initiation.
S66245, S64500, S66243 Genomic DNA. Translation: AAB20293.1.
X52466 mRNA. Translation: CAA36704.1. Different initiation.
M85170 mRNA. Translation: AAA40124.1. Different initiation.
M96430
, M96426, M96427, M96428 Genomic DNA. Translation: AAA37163.1.
CCDSiCCDS27755.1.
PIRiA37344.
JX0172.
RefSeqiNP_038483.1. NM_013455.3.
UniGeneiMm.57.

3D structure databases

ProteinModelPortaliP23578.
SMRiP23578. Positions 43-303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197930. 1 interaction.
STRINGi10090.ENSMUSP00000023295.

Protein family/group databases

MEROPSiS01.223.

Proteomic databases

PaxDbiP23578.
PRIDEiP23578.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023295; ENSMUSP00000023295; ENSMUSG00000022622.
GeneIDi11434.
UCSCiuc007xhc.2. mouse.

Organism-specific databases

CTDi49.
MGIiMGI:87884. Acr.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00800000124034.
HOGENOMiHOG000033792.
HOVERGENiHBG013304.
InParanoidiP23578.
OMAiKKVYDWR.
OrthoDBiEOG75B84T.
PhylomeDBiP23578.
TreeFamiTF335943.

Enzyme and pathway databases

ReactomeiREACT_279120. Acrosome Reaction.

Miscellaneous databases

NextBioi278728.
PROiP23578.
SOURCEiSearch...

Gene expression databases

BgeeiP23578.
CleanExiMM_ACR.
ExpressionAtlasiP23578. baseline.
GenevisibleiP23578. MM.

Family and domain databases

InterProiIPR012267. Pept_S1A_acrosin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PANTHERiPTHR24252. PTHR24252. 1 hit.
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001141. Acrosin. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of mouse proacrosin deduced from the cDNA sequence and its gene expression during spermatogenesis."
    Kashiwabara S., Baba T., Takada M., Watanabe K., Yano Y., Arai Y.
    J. Biochem. 108:785-791(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Structure and organization of the mouse acrosin gene."
    Watanabe K., Baba T., Kashiwabara S., Okamoto A., Arai Y.
    J. Biochem. 109:828-833(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Mouse preproacrosin: cDNA sequence, primary structure and postmeiotic expression in spermatogenesis."
    Klemm U., Maier W.-M., Tsaousidou S., Adham I.M., Willison K., Engel W.
    Differentiation 42:160-166(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "Mouse proacrosin gene: nucleotide sequence, diploid expression, and chromosomal localization."
    Kremling H., Keime S., Wilhelm K., Adham I.M., Hameister H., Engel W.
    Genomics 11:828-834(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiACRO_MOUSE
AccessioniPrimary (citable) accession number: P23578
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 22, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.