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Protein

Acrosin

Gene

Acr

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulationi

Inhibited by SERPINA5.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei89Charge relay systemBy similarity1
Active sitei143Charge relay systemBy similarity1
Active sitei241Charge relay systemBy similarity1

GO - Molecular functioni

  • amidase activity Source: UniProtKB
  • drug binding Source: UniProtKB
  • fucose binding Source: UniProtKB
  • mannose binding Source: UniProtKB
  • peptidase activity Source: MGI
  • serine-type endopeptidase activity Source: UniProtKB

GO - Biological processi

  • acrosome reaction Source: UniProtKB
  • activation of adenylate cyclase activity Source: UniProtKB
  • binding of sperm to zona pellucida Source: MGI
  • penetration of zona pellucida Source: MGI
  • response to steroid hormone Source: Ensembl
  • single fertilization Source: UniProtKB

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Enzyme and pathway databases

ReactomeiR-MMU-1300645 Acrosome Reaction

Protein family/group databases

MEROPSiS01.223

Names & Taxonomyi

Protein namesi
Recommended name:
Acrosin (EC:3.4.21.10)
Cleaved into the following 2 chains:
Gene namesi
Name:Acr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:87884 Acr

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Add BLAST19
ChainiPRO_000002752220 – 436AcrosinAdd BLAST417
ChainiPRO_000002752320 – 42Acrosin light chainAdd BLAST23
ChainiPRO_000002752443 – 345Acrosin heavy chainAdd BLAST303
PropeptideiPRO_0000027525346 – 436Pro-richBy similarityAdd BLAST91

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi22N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi25 ↔ 155Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi29 ↔ 163Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
Disulfide bondi178 ↔ 247PROSITE-ProRule annotation
Disulfide bondi210 ↔ 226PROSITE-ProRule annotation
Glycosylationi211N-linked (GlcNAc...) asparagineBy similarity1
Disulfide bondi237 ↔ 267PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiP23578
PRIDEiP23578

Expressioni

Gene expression databases

BgeeiENSMUSG00000022622
CleanExiMM_ACR
GenevisibleiP23578 MM

Interactioni

Subunit structurei

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 (By similarity).By similarity

Protein-protein interaction databases

BioGridi197930, 1 interactor
STRINGi10090.ENSMUSP00000023295

Structurei

3D structure databases

ProteinModelPortaliP23578
SMRiP23578
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 291Peptidase S1PROSITE-ProRule annotationAdd BLAST249

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00900000140784
HOGENOMiHOG000033792
HOVERGENiHBG013304
InParanoidiP23578
KOiK01317
OMAiDWRLIFG
OrthoDBiEOG091G0AH5
PhylomeDBiP23578
TreeFamiTF335943

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR012267 Pept_S1A_acrosin
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PANTHERiPTHR24252 PTHR24252, 1 hit
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001141 Acrosin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23578-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEMLPTVAV LVLAVSVVAK DNTTCDGPCG LRFRQNSQAG TRIVSGQSAQ
60 70 80 90 100
LGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW
110 120 130 140 150
RLVFGAQEIE YGRNKPVKEP QQERYVQKIV IHEKYNVVTE GNDIALLKIT
160 170 180 190 200
PPVTCGNFIG PCCLPHFKAG PPQIPHTCYV TGWGYIKEKA PRPSPVLMEA
210 220 230 240 250
RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDN
260 270 280 290 300
VDSPFVVVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPNALHLIQP
310 320 330 340 350
ATPHPPTTRH PMVSFHPPSL RPPWYFQHLP SRPLYLRPLR PLLHRPSSTQ
360 370 380 390 400
TSSSLMPLLS PPTPAQPASF TIATQHMRHR TTLSFARRLQ RLIEALKMRT
410 420 430
YPMKHPSQYS GPRNYHYRFS TFEPLSNKPS EPFLHS
Length:436
Mass (Da):48,929
Last modified:November 1, 1991 - v1
Checksum:i4CC6EAD56CB51F54
GO

Sequence cautioni

The sequence AAA40124 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA00651 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAA36704 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8Missing in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti8Missing in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti10 – 14VLVLA → FWSVK in CAA36704 (PubMed:2111255).Curated5
Sequence conflicti10 – 14VLVLA → FWSVK in AAA40124 (PubMed:2111255).Curated5
Sequence conflicti17 – 18VV → AG in CAA36704 (PubMed:2111255).Curated2
Sequence conflicti17 – 18VV → AG in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti23T → A in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti23T → A in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti26D → F in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti26D → F in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti33F → T in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti33F → T in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti50 – 51QL → HV in CAA36704 (PubMed:2111255).Curated2
Sequence conflicti50 – 51QL → HV in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti122Q → E in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti122Q → E in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti122Q → E (PubMed:1783391).Curated1
Sequence conflicti149I → V in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti149I → V in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti149I → V (PubMed:1783391).Curated1
Sequence conflicti173 – 174QI → RNT in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti173Q → RK in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti177 – 183TCYVTGW → LLRDWV in AAA40124 (PubMed:2111255).Curated7
Sequence conflicti185 – 186YI → IH in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti188 – 189EK → RE in CAA36704 (PubMed:2111255).Curated2
Sequence conflicti188 – 189EK → RE in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti251 – 253VDS → ARQ in CAA36704 (PubMed:2111255).Curated3
Sequence conflicti251 – 253VDS → ARQ in AAA40124 (PubMed:2111255).Curated3
Sequence conflicti255 – 268FVVVG…GVGCA → LCGRGDHELGGRLC in AAA40124 (PubMed:2111255).CuratedAdd BLAST14
Sequence conflicti255 – 268FVVVG…GVGCA → LCGRGDHELGGRLC in AAA37163 (PubMed:1783391).CuratedAdd BLAST14
Sequence conflicti299 – 300QP → PA in CAA36704 (PubMed:2111255).Curated2
Sequence conflicti299 – 300QP → PA in AAA40124 (PubMed:2111255).Curated2
Sequence conflicti320L → F in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti320L → F in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti405H → Y in CAA36704 (PubMed:2111255).Curated1
Sequence conflicti405H → Y in AAA40124 (PubMed:2111255).Curated1
Sequence conflicti407 – 436SQYSG…PFLHS → PSTVDKELPLPLLHV in CAA36704 (PubMed:2111255).CuratedAdd BLAST30
Sequence conflicti407 – 436SQYSG…PFLHS → PSTVDKELPLPLLHV in AAA40124 (PubMed:2111255).CuratedAdd BLAST30
Sequence conflicti407 – 436SQYSG…PFLHS → PSTVDKELPLPLLHV (PubMed:1783391).CuratedAdd BLAST30

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D00754 mRNA Translation: BAA00651.1 Different initiation.
S66245, S64500, S66243 Genomic DNA Translation: AAB20293.1
X52466 mRNA Translation: CAA36704.1 Different initiation.
M85170 mRNA Translation: AAA40124.1 Different initiation.
M96430
, M96426, M96427, M96428 Genomic DNA Translation: AAA37163.1
CCDSiCCDS27755.1
PIRiA37344
JX0172
RefSeqiNP_038483.1, NM_013455.3
UniGeneiMm.57

Genome annotation databases

EnsembliENSMUST00000023295; ENSMUSP00000023295; ENSMUSG00000022622
GeneIDi11434
KEGGimmu:11434
UCSCiuc007xhc.2 mouse

Similar proteinsi

Entry informationi

Entry nameiACRO_MOUSE
AccessioniPrimary (citable) accession number: P23578
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 25, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health