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P23578

- ACRO_MOUSE

UniProt

P23578 - ACRO_MOUSE

Protein

Acrosin

Gene

Acr

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Enzyme regulationi

    Inhibited by SERPINA5.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891Charge relay systemBy similarity
    Active sitei143 – 1431Charge relay systemBy similarity
    Active sitei241 – 2411Charge relay systemBy similarity

    GO - Molecular functioni

    1. amidase activity Source: UniProtKB
    2. drug binding Source: UniProtKB
    3. fucose binding Source: UniProtKB
    4. mannose binding Source: UniProtKB
    5. peptidase activity Source: MGI
    6. protein binding Source: MGI
    7. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. acrosome reaction Source: UniProtKB
    2. activation of adenylate cyclase activity Source: UniProtKB
    3. binding of sperm to zona pellucida Source: MGI
    4. penetration of zona pellucida Source: MGI
    5. response to steroid hormone Source: Ensembl
    6. single fertilization Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    ReactomeiREACT_199073. Acrosome Reaction.

    Protein family/group databases

    MEROPSiS01.223.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acrosin (EC:3.4.21.10)
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Acr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:87884. Acr.

    Subcellular locationi

    GO - Cellular componenti

    1. acrosomal matrix Source: HGNC
    2. acrosomal vesicle Source: MGI
    3. Golgi-associated vesicle Source: Ensembl
    4. protein complex Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Add
    BLAST
    Chaini20 – 436417AcrosinPRO_0000027522Add
    BLAST
    Chaini20 – 4223Acrosin light chainPRO_0000027523Add
    BLAST
    Chaini43 – 345303Acrosin heavy chainPRO_0000027524Add
    BLAST
    Propeptidei346 – 43691Pro-richBy similarityPRO_0000027525Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi22 – 221N-linked (GlcNAc...)By similarity
    Disulfide bondi25 ↔ 155Interchain (between light and heavy chains)PROSITE-ProRule annotation
    Disulfide bondi29 ↔ 163Interchain (between light and heavy chains)PROSITE-ProRule annotation
    Disulfide bondi74 ↔ 90PROSITE-ProRule annotation
    Disulfide bondi178 ↔ 247PROSITE-ProRule annotation
    Disulfide bondi210 ↔ 226PROSITE-ProRule annotation
    Glycosylationi211 – 2111N-linked (GlcNAc...)By similarity
    Disulfide bondi237 ↔ 267PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    PaxDbiP23578.
    PRIDEiP23578.

    Expressioni

    Gene expression databases

    BgeeiP23578.
    CleanExiMM_ACR.
    GenevestigatoriP23578.

    Interactioni

    Subunit structurei

    Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi197930. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP23578.
    SMRiP23578. Positions 43-303.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini43 – 291249Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00750000117343.
    HOGENOMiHOG000033792.
    HOVERGENiHBG013304.
    InParanoidiP23578.
    KOiK01317.
    OMAiGSNALHM.
    OrthoDBiEOG75B84T.
    PhylomeDBiP23578.
    TreeFamiTF335943.

    Family and domain databases

    InterProiIPR012267. Pept_S1A_acrosin.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001141. Acrosin. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23578-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEMLPTVAV LVLAVSVVAK DNTTCDGPCG LRFRQNSQAG TRIVSGQSAQ    50
    LGAWPWMVSL QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW 100
    RLVFGAQEIE YGRNKPVKEP QQERYVQKIV IHEKYNVVTE GNDIALLKIT 150
    PPVTCGNFIG PCCLPHFKAG PPQIPHTCYV TGWGYIKEKA PRPSPVLMEA 200
    RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD SGGPLMCRDN 250
    VDSPFVVVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPNALHLIQP 300
    ATPHPPTTRH PMVSFHPPSL RPPWYFQHLP SRPLYLRPLR PLLHRPSSTQ 350
    TSSSLMPLLS PPTPAQPASF TIATQHMRHR TTLSFARRLQ RLIEALKMRT 400
    YPMKHPSQYS GPRNYHYRFS TFEPLSNKPS EPFLHS 436
    Length:436
    Mass (Da):48,929
    Last modified:November 1, 1991 - v1
    Checksum:i4CC6EAD56CB51F54
    GO

    Sequence cautioni

    The sequence AAA40124.1 differs from that shown. Reason: Erroneous initiation.
    The sequence BAA00651.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAA36704.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti8 – 81Missing in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti8 – 81Missing in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti10 – 145VLVLA → FWSVK in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti10 – 145VLVLA → FWSVK in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti17 – 182VV → AG in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti17 – 182VV → AG in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti23 – 231T → A in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti23 – 231T → A in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti26 – 261D → F in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti26 – 261D → F in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti33 – 331F → T in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti33 – 331F → T in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti50 – 512QL → HV in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti50 – 512QL → HV in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti122 – 1221Q → E in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti122 – 1221Q → E in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti122 – 1221Q → E(PubMed:1783391)Curated
    Sequence conflicti149 – 1491I → V in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti149 – 1491I → V in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti149 – 1491I → V(PubMed:1783391)Curated
    Sequence conflicti173 – 1742QI → RNT in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti173 – 1731Q → RK in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti177 – 1837TCYVTGW → LLRDWV in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti185 – 1862YI → IH in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti188 – 1892EK → RE in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti188 – 1892EK → RE in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti251 – 2533VDS → ARQ in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti251 – 2533VDS → ARQ in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA40124. (PubMed:2111255)CuratedAdd
    BLAST
    Sequence conflicti255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA37163. (PubMed:1783391)CuratedAdd
    BLAST
    Sequence conflicti299 – 3002QP → PA in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti299 – 3002QP → PA in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti320 – 3201L → F in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti320 – 3201L → F in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti405 – 4051H → Y in CAA36704. (PubMed:2111255)Curated
    Sequence conflicti405 – 4051H → Y in AAA40124. (PubMed:2111255)Curated
    Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in CAA36704. (PubMed:2111255)CuratedAdd
    BLAST
    Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in AAA40124. (PubMed:2111255)CuratedAdd
    BLAST
    Sequence conflicti407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV(PubMed:1783391)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00754 mRNA. Translation: BAA00651.1. Different initiation.
    S66245, S64500, S66243 Genomic DNA. Translation: AAB20293.1.
    X52466 mRNA. Translation: CAA36704.1. Different initiation.
    M85170 mRNA. Translation: AAA40124.1. Different initiation.
    M96430
    , M96426, M96427, M96428 Genomic DNA. Translation: AAA37163.1.
    CCDSiCCDS27755.1.
    PIRiA37344.
    JX0172.
    RefSeqiNP_038483.1. NM_013455.3.
    UniGeneiMm.57.

    Genome annotation databases

    EnsembliENSMUST00000023295; ENSMUSP00000023295; ENSMUSG00000022622.
    GeneIDi11434.
    KEGGimmu:11434.
    UCSCiuc007xhc.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D00754 mRNA. Translation: BAA00651.1 . Different initiation.
    S66245 , S64500 , S66243 Genomic DNA. Translation: AAB20293.1 .
    X52466 mRNA. Translation: CAA36704.1 . Different initiation.
    M85170 mRNA. Translation: AAA40124.1 . Different initiation.
    M96430
    , M96426 , M96427 , M96428 Genomic DNA. Translation: AAA37163.1 .
    CCDSi CCDS27755.1.
    PIRi A37344.
    JX0172.
    RefSeqi NP_038483.1. NM_013455.3.
    UniGenei Mm.57.

    3D structure databases

    ProteinModelPortali P23578.
    SMRi P23578. Positions 43-303.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 197930. 1 interaction.

    Protein family/group databases

    MEROPSi S01.223.

    Proteomic databases

    PaxDbi P23578.
    PRIDEi P23578.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023295 ; ENSMUSP00000023295 ; ENSMUSG00000022622 .
    GeneIDi 11434.
    KEGGi mmu:11434.
    UCSCi uc007xhc.2. mouse.

    Organism-specific databases

    CTDi 49.
    MGIi MGI:87884. Acr.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00750000117343.
    HOGENOMi HOG000033792.
    HOVERGENi HBG013304.
    InParanoidi P23578.
    KOi K01317.
    OMAi GSNALHM.
    OrthoDBi EOG75B84T.
    PhylomeDBi P23578.
    TreeFami TF335943.

    Enzyme and pathway databases

    Reactomei REACT_199073. Acrosome Reaction.

    Miscellaneous databases

    NextBioi 278728.
    PROi P23578.
    SOURCEi Search...

    Gene expression databases

    Bgeei P23578.
    CleanExi MM_ACR.
    Genevestigatori P23578.

    Family and domain databases

    InterProi IPR012267. Pept_S1A_acrosin.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001141. Acrosin. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of mouse proacrosin deduced from the cDNA sequence and its gene expression during spermatogenesis."
      Kashiwabara S., Baba T., Takada M., Watanabe K., Yano Y., Arai Y.
      J. Biochem. 108:785-791(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Structure and organization of the mouse acrosin gene."
      Watanabe K., Baba T., Kashiwabara S., Okamoto A., Arai Y.
      J. Biochem. 109:828-833(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Mouse preproacrosin: cDNA sequence, primary structure and postmeiotic expression in spermatogenesis."
      Klemm U., Maier W.-M., Tsaousidou S., Adham I.M., Willison K., Engel W.
      Differentiation 42:160-166(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "Mouse proacrosin gene: nucleotide sequence, diploid expression, and chromosomal localization."
      Kremling H., Keime S., Wilhelm K., Adham I.M., Hameister H., Engel W.
      Genomics 11:828-834(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiACRO_MOUSE
    AccessioniPrimary (citable) accession number: P23578
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3