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P23578 (ACRO_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Acrosin

EC=3.4.21.10

Cleaved into the following 2 chains:

  1. Acrosin light chain
  2. Acrosin heavy chain
Gene names
Name:Acr
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length436 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acrosin is the major protease of mammalian spermatozoa. It is a serine protease of trypsin-like cleavage specificity, it is synthesized in a zymogen form, proacrosin and stored in the acrosome.

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Enzyme regulation

Inhibited by SERPINA5 By similarity.

Subunit structure

Heavy chain (catalytic) and a light chain linked by two disulfide bonds. Forms a heterodimer with SERPINA5 By similarity.

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Sequence caution

The sequence AAA40124.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAA00651.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAA36704.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   DomainSignal
   Molecular functionHydrolase
Protease
Serine protease
   PTMDisulfide bond
Glycoprotein
Zymogen
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processacrosome reaction

Inferred from sequence or structural similarity. Source: UniProtKB

activation of adenylate cyclase activity

Inferred from sequence or structural similarity. Source: UniProtKB

binding of sperm to zona pellucida

Inferred from genetic interaction PubMed 15685642. Source: MGI

penetration of zona pellucida

Inferred from mutant phenotype PubMed 7989357PubMed 9041140. Source: MGI

response to steroid hormone

Inferred from electronic annotation. Source: Ensembl

single fertilization

Inferred from mutant phenotype PubMed 15685642. Source: UniProtKB

   Cellular_componentGolgi-associated vesicle

Inferred from electronic annotation. Source: Ensembl

acrosomal matrix

Traceable author statement PubMed 12398221. Source: HGNC

acrosomal vesicle

Inferred from direct assay PubMed 7798216. Source: MGI

protein complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionamidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

drug binding

Inferred from sequence or structural similarity. Source: UniProtKB

fucose binding

Inferred from sequence or structural similarity. Source: UniProtKB

mannose binding

Inferred from sequence or structural similarity. Source: UniProtKB

peptidase activity

Inferred from mutant phenotype PubMed 7989357PubMed 9041140. Source: MGI

protein binding

Inferred from physical interaction PubMed 11739644. Source: MGI

serine-type endopeptidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919
Chain20 – 436417Acrosin
PRO_0000027522
Chain20 – 4223Acrosin light chain
PRO_0000027523
Chain43 – 345303Acrosin heavy chain
PRO_0000027524
Propeptide346 – 43691Pro-rich By similarity
PRO_0000027525

Regions

Domain43 – 291249Peptidase S1

Sites

Active site891Charge relay system By similarity
Active site1431Charge relay system By similarity
Active site2411Charge relay system By similarity

Amino acid modifications

Glycosylation221N-linked (GlcNAc...) By similarity
Glycosylation2111N-linked (GlcNAc...) By similarity
Disulfide bond25 ↔ 155Interchain (between light and heavy chains) By similarity
Disulfide bond29 ↔ 163Interchain (between light and heavy chains) By similarity
Disulfide bond74 ↔ 90 By similarity
Disulfide bond178 ↔ 247 By similarity
Disulfide bond210 ↔ 226 By similarity
Disulfide bond237 ↔ 267 By similarity

Experimental info

Sequence conflict81Missing in CAA36704. Ref.3
Sequence conflict81Missing in AAA40124. Ref.3
Sequence conflict10 – 145VLVLA → FWSVK in CAA36704. Ref.3
Sequence conflict10 – 145VLVLA → FWSVK in AAA40124. Ref.3
Sequence conflict17 – 182VV → AG in CAA36704. Ref.3
Sequence conflict17 – 182VV → AG in AAA40124. Ref.3
Sequence conflict231T → A in CAA36704. Ref.3
Sequence conflict231T → A in AAA40124. Ref.3
Sequence conflict261D → F in CAA36704. Ref.3
Sequence conflict261D → F in AAA40124. Ref.3
Sequence conflict331F → T in CAA36704. Ref.3
Sequence conflict331F → T in AAA40124. Ref.3
Sequence conflict50 – 512QL → HV in CAA36704. Ref.3
Sequence conflict50 – 512QL → HV in AAA40124. Ref.3
Sequence conflict1221Q → E in CAA36704. Ref.3
Sequence conflict1221Q → E in AAA40124. Ref.3
Sequence conflict1221Q → E Ref.4
Sequence conflict1491I → V in CAA36704. Ref.3
Sequence conflict1491I → V in AAA40124. Ref.3
Sequence conflict1491I → V Ref.4
Sequence conflict173 – 1742QI → RNT in AAA40124. Ref.3
Sequence conflict1731Q → RK in CAA36704. Ref.3
Sequence conflict177 – 1837TCYVTGW → LLRDWV in AAA40124. Ref.3
Sequence conflict185 – 1862YI → IH in AAA40124. Ref.3
Sequence conflict188 – 1892EK → RE in CAA36704. Ref.3
Sequence conflict188 – 1892EK → RE in AAA40124. Ref.3
Sequence conflict251 – 2533VDS → ARQ in CAA36704. Ref.3
Sequence conflict251 – 2533VDS → ARQ in AAA40124. Ref.3
Sequence conflict255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA40124. Ref.3
Sequence conflict255 – 26814FVVVG…GVGCA → LCGRGDHELGGRLC in AAA37163. Ref.4
Sequence conflict299 – 3002QP → PA in CAA36704. Ref.3
Sequence conflict299 – 3002QP → PA in AAA40124. Ref.3
Sequence conflict3201L → F in CAA36704. Ref.3
Sequence conflict3201L → F in AAA40124. Ref.3
Sequence conflict4051H → Y in CAA36704. Ref.3
Sequence conflict4051H → Y in AAA40124. Ref.3
Sequence conflict407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in CAA36704. Ref.3
Sequence conflict407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV in AAA40124. Ref.3
Sequence conflict407 – 43630SQYSG…PFLHS → PSTVDKELPLPLLHV Ref.4

Sequences

Sequence LengthMass (Da)Tools
P23578 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 4CC6EAD56CB51F54

FASTA43648,929
        10         20         30         40         50         60 
MVEMLPTVAV LVLAVSVVAK DNTTCDGPCG LRFRQNSQAG TRIVSGQSAQ LGAWPWMVSL 

        70         80         90        100        110        120 
QIFTSHNSRR YHACGGSLLN SHWVLTAAHC FDNKKKVYDW RLVFGAQEIE YGRNKPVKEP 

       130        140        150        160        170        180 
QQERYVQKIV IHEKYNVVTE GNDIALLKIT PPVTCGNFIG PCCLPHFKAG PPQIPHTCYV 

       190        200        210        220        230        240 
TGWGYIKEKA PRPSPVLMEA RVDLIDLDLC NSTQWYNGRV TSTNVCAGYP EGKIDTCQGD 

       250        260        270        280        290        300 
SGGPLMCRDN VDSPFVVVGI TSWGVGCARA KRPGVYTATW DYLDWIASKI GPNALHLIQP 

       310        320        330        340        350        360 
ATPHPPTTRH PMVSFHPPSL RPPWYFQHLP SRPLYLRPLR PLLHRPSSTQ TSSSLMPLLS 

       370        380        390        400        410        420 
PPTPAQPASF TIATQHMRHR TTLSFARRLQ RLIEALKMRT YPMKHPSQYS GPRNYHYRFS 

       430 
TFEPLSNKPS EPFLHS 

« Hide

References

[1]"Primary structure of mouse proacrosin deduced from the cDNA sequence and its gene expression during spermatogenesis."
Kashiwabara S., Baba T., Takada M., Watanabe K., Yano Y., Arai Y.
J. Biochem. 108:785-791(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Structure and organization of the mouse acrosin gene."
Watanabe K., Baba T., Kashiwabara S., Okamoto A., Arai Y.
J. Biochem. 109:828-833(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Mouse preproacrosin: cDNA sequence, primary structure and postmeiotic expression in spermatogenesis."
Klemm U., Maier W.-M., Tsaousidou S., Adham I.M., Willison K., Engel W.
Differentiation 42:160-166(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Mouse proacrosin gene: nucleotide sequence, diploid expression, and chromosomal localization."
Kremling H., Keime S., Wilhelm K., Adham I.M., Hameister H., Engel W.
Genomics 11:828-834(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D00754 mRNA. Translation: BAA00651.1. Different initiation.
S66245, S64500, S66243 Genomic DNA. Translation: AAB20293.1.
X52466 mRNA. Translation: CAA36704.1. Different initiation.
M85170 mRNA. Translation: AAA40124.1. Different initiation.
M96430 expand/collapse EMBL AC list , M96426, M96427, M96428 Genomic DNA. Translation: AAA37163.1.
CCDSCCDS27755.1.
PIRA37344.
JX0172.
RefSeqNP_038483.1. NM_013455.3.
UniGeneMm.57.

3D structure databases

ProteinModelPortalP23578.
SMRP23578. Positions 43-303.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid197930. 1 interaction.

Protein family/group databases

MEROPSS01.223.

Proteomic databases

PaxDbP23578.
PRIDEP23578.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023295; ENSMUSP00000023295; ENSMUSG00000022622.
GeneID11434.
KEGGmmu:11434.
UCSCuc007xhc.2. mouse.

Organism-specific databases

CTD49.
MGIMGI:87884. Acr.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00750000117343.
HOGENOMHOG000033792.
HOVERGENHBG013304.
InParanoidP23578.
KOK01317.
OMAGSNALHM.
OrthoDBEOG75B84T.
PhylomeDBP23578.
TreeFamTF335943.

Gene expression databases

BgeeP23578.
CleanExMM_ACR.
GenevestigatorP23578.

Family and domain databases

InterProIPR012267. Pept_S1A_acrosin.
IPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFPIRSF001141. Acrosin. 1 hit.
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio278728.
PROP23578.
SOURCESearch...

Entry information

Entry nameACRO_MOUSE
AccessionPrimary (citable) accession number: P23578
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot