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P23577

- CYF_CHLRE

UniProt

P23577 - CYF_CHLRE

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Protein
Apocytochrome f
Gene
petA
Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.UniRule annotation

Cofactori

Binds 1 heme group covalently.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi32 – 321Iron (heme axial ligand); via amino nitrogen
Binding sitei52 – 521Heme (covalent)
Binding sitei55 – 551Heme (covalent)
Metal bindingi56 – 561Iron (heme axial ligand)

GO - Molecular functioni

  1. electron carrier activity Source: UniProtKB-HAMAP
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. oxidation-reduction process Source: UniProtKB-HAMAP
  2. photosynthesis Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Biological processi

Electron transport, Photosynthesis, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciCHLAMY:CHRECP001-MONOMER.
MetaCyc:CHRECP001-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Apocytochrome f
Gene namesi
Name:petA
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
ProteomesiUP000006906: Chloroplast

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei283 – 30220Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
  2. integral component of thylakoid membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 31311 Publication
Add
BLAST
Chaini32 – 317286Apocytochrome fUniRule annotation
PRO_0000023809Add
BLAST

Proteomic databases

PRIDEiP23577.

Interactioni

Subunit structurei

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Protein-protein interaction databases

DIPiDIP-48404N.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi33 – 397
Beta strandi41 – 444
Helixi51 – 544
Beta strandi63 – 653
Beta strandi68 – 703
Beta strandi75 – 817
Beta strandi86 – 883
Beta strandi95 – 984
Beta strandi101 – 1077
Helixi117 – 1193
Helixi122 – 1287
Beta strandi134 – 1374
Beta strandi143 – 1508
Helixi151 – 1544
Beta strandi155 – 1628
Turni166 – 1683
Beta strandi174 – 18613
Beta strandi198 – 2003
Beta strandi208 – 22013
Beta strandi223 – 2286
Beta strandi230 – 2323
Beta strandi234 – 2396
Beta strandi241 – 2433
Beta strandi258 – 2603
Beta strandi267 – 27711
Helixi280 – 31435

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CFMX-ray2.00A/B/C32-282[»]
1E2VX-ray1.85A/B/C32-282[»]
1E2WX-ray1.60A/B32-282[»]
1E2ZX-ray2.50A/B/C32-282[»]
1EWHX-ray2.35A/B/C32-282[»]
1Q90X-ray3.10A32-317[»]
ProteinModelPortaliP23577.
SMRiP23577. Positions 32-317.

Miscellaneous databases

EvolutionaryTraceiP23577.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome f family.

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

KOiK02634.

Family and domain databases

Gene3Di1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPiMF_00610. Cytb6_f_cytF.
InterProiIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01333. Apocytochr_F_C. 1 hit.
[Graphical view]
PRINTSiPR00610. CYTOCHROMEF.
SUPFAMiSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEiPS51010. CYTF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23577-1 [UniParc]FASTAAdd to Basket

« Hide

MSNQVFTTLR AATLAVILGM AGGLAVSPAQ AYPVFAQQNY ANPREANGRI    50
VCANCHLAQK AVEIEVPQAV LPDTVFEAVI ELPYDKQVKQ VLANGKKGDL 100
NVGMVLILPE GFELAPPDRV PAEIKEKVGN LYYQPYSPEQ KNILVVGPVP 150
GKKYSEMVVP ILSPDPAKNK NVSYLKYPIY FGGNRGRGQV YPDGKKSNNT 200
IYNASAAGKI VAITALSEKK GGFEVSIEKA NGEVVVDKIP AGPDLIVKEG 250
QTVQADQPLT NNPNVGGFGQ AETEIVLQNP ARIQGLLVFF SFVLLTQVLL 300
VLKKKQFEKV QLAEMNF 317
Length:317
Mass (Da):34,292
Last modified:November 1, 1991 - v1
Checksum:i1DC74E50552B7A53
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti53 – 531A → N AA sequence 1 Publication
Sequence conflicti64 – 641I → S in CAA40911. 1 Publication
Sequence conflicti89 – 913KQV → NS in CAA40911. 1 Publication
Sequence conflicti132 – 1321Y → F in CAA40911. 1 Publication
Sequence conflicti165 – 1651D → Y1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72917 Genomic DNA. Translation: CAA51422.1.
D01036 Genomic DNA. Translation: BAA00844.1.
X57744 Genomic DNA. Translation: CAA40911.1.
FJ423446 Genomic DNA. Translation: ACJ50091.1.
BK000554 Genomic DNA. Translation: DAA00904.1.
PIRiS16916.
RefSeqiNP_958358.1. NC_005353.1.

Genome annotation databases

EnsemblPlantsiDAA00904; DAA00904; DAA00904.
GeneIDi2716989.
KEGGicre:ChreCp001.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X72917 Genomic DNA. Translation: CAA51422.1 .
D01036 Genomic DNA. Translation: BAA00844.1 .
X57744 Genomic DNA. Translation: CAA40911.1 .
FJ423446 Genomic DNA. Translation: ACJ50091.1 .
BK000554 Genomic DNA. Translation: DAA00904.1 .
PIRi S16916.
RefSeqi NP_958358.1. NC_005353.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1CFM X-ray 2.00 A/B/C 32-282 [» ]
1E2V X-ray 1.85 A/B/C 32-282 [» ]
1E2W X-ray 1.60 A/B 32-282 [» ]
1E2Z X-ray 2.50 A/B/C 32-282 [» ]
1EWH X-ray 2.35 A/B/C 32-282 [» ]
1Q90 X-ray 3.10 A 32-317 [» ]
ProteinModelPortali P23577.
SMRi P23577. Positions 32-317.
ModBasei Search...

Protein-protein interaction databases

DIPi DIP-48404N.

Proteomic databases

PRIDEi P23577.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi DAA00904 ; DAA00904 ; DAA00904 .
GeneIDi 2716989.
KEGGi cre:ChreCp001.

Phylogenomic databases

KOi K02634.

Enzyme and pathway databases

BioCyci CHLAMY:CHRECP001-MONOMER.
MetaCyc:CHRECP001-MONOMER.

Miscellaneous databases

EvolutionaryTracei P23577.

Family and domain databases

Gene3Di 1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPi MF_00610. Cytb6_f_cytF.
InterProi IPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01333. Apocytochr_F_C. 1 hit.
[Graphical view ]
PRINTSi PR00610. CYTOCHROMEF.
SUPFAMi SSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEi PS51010. CYTF. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii."
    Bueschlen S., Choquet Y., Kuras R., Wollman F.A.
    FEBS Lett. 284:257-262(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 137c / CC-125.
  2. "Structural analysis and expression during dark-light transitions of a gene for cytochrome f in Chlamydomonas reinhardtii."
    Matsumoto T., Matsuo M., Matsuda Y.
    Plant Cell Physiol. 32:863-872(1991)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cw15.
  3. "Nucleotide sequence of the petA (cytochrome f) gene from the green alga, Chlamydomonas reinhardtii."
    Bertsch J., Malkin R.
    Plant Mol. Biol. 17:131-133(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
    Smith D.R., Lee R.W.
    BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC-503.
  5. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
    Pierre Y., Breyton C., Kramer D., Popot J.-L.
    J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 32-53, CHARACTERIZATION.
    Strain: WT12.
  6. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
    Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
    Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
  7. "X-ray structure of a truncated form of cytochrome f from Chlamydomonas reinhardtii."
    Chi Y.-I., Huang L.-S., Zhang Z., Fernandez-Velasco J.G., Berry E.A.
    Biochemistry 39:7689-7701(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
  8. "Interruption of the internal water chain of cytochrome f impairs photosynthetic function."
    Sainz G., Carrell C.J., Ponamarev M.V., Soriano G.M., Cramer W.A., Smith J.L.
    Biochemistry 39:9164-9173(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

Entry informationi

Entry nameiCYF_CHLRE
AccessioniPrimary (citable) accession number: P23577
Secondary accession number(s): B7U1E4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 14, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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