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P23577 (CYF_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Apocytochrome f
Gene names
Name:petA
Encoded onPlastid; Chloroplast
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii) [Reference proteome]
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions. HAMAP-Rule MF_00610

Cofactor

Binds 1 heme group covalently.

Subunit structure

The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

Subcellular location

Plastidchloroplast thylakoid membrane; Single-pass membrane protein HAMAP-Rule MF_00610.

Sequence similarities

Belongs to the cytochrome f family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3131 Ref.5
Chain32 – 317286Apocytochrome f HAMAP-Rule MF_00610
PRO_0000023809

Regions

Transmembrane283 – 30220Helical; Potential

Sites

Metal binding321Iron (heme axial ligand); via amino nitrogen
Metal binding561Iron (heme axial ligand)
Binding site521Heme (covalent)
Binding site551Heme (covalent)

Experimental info

Sequence conflict531A → N AA sequence Ref.5
Sequence conflict641I → S in CAA40911. Ref.3
Sequence conflict89 – 913KQV → NS in CAA40911. Ref.3
Sequence conflict1321Y → F in CAA40911. Ref.3
Sequence conflict1651D → Y Ref.1

Secondary structure

................................................... 317
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23577 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 1DC74E50552B7A53

FASTA31734,292
        10         20         30         40         50         60 
MSNQVFTTLR AATLAVILGM AGGLAVSPAQ AYPVFAQQNY ANPREANGRI VCANCHLAQK 

        70         80         90        100        110        120 
AVEIEVPQAV LPDTVFEAVI ELPYDKQVKQ VLANGKKGDL NVGMVLILPE GFELAPPDRV 

       130        140        150        160        170        180 
PAEIKEKVGN LYYQPYSPEQ KNILVVGPVP GKKYSEMVVP ILSPDPAKNK NVSYLKYPIY 

       190        200        210        220        230        240 
FGGNRGRGQV YPDGKKSNNT IYNASAAGKI VAITALSEKK GGFEVSIEKA NGEVVVDKIP 

       250        260        270        280        290        300 
AGPDLIVKEG QTVQADQPLT NNPNVGGFGQ AETEIVLQNP ARIQGLLVFF SFVLLTQVLL 

       310 
VLKKKQFEKV QLAEMNF 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii."
Bueschlen S., Choquet Y., Kuras R., Wollman F.A.
FEBS Lett. 284:257-262(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 137c / CC-125.
[2]"Structural analysis and expression during dark-light transitions of a gene for cytochrome f in Chlamydomonas reinhardtii."
Matsumoto T., Matsuo M., Matsuda Y.
Plant Cell Physiol. 32:863-872(1991)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cw15.
[3]"Nucleotide sequence of the petA (cytochrome f) gene from the green alga, Chlamydomonas reinhardtii."
Bertsch J., Malkin R.
Plant Mol. Biol. 17:131-133(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
Smith D.R., Lee R.W.
BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CC-503.
[5]"Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
Pierre Y., Breyton C., Kramer D., Popot J.-L.
J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 32-53, CHARACTERIZATION.
Strain: WT12.
[6]"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
[7]"X-ray structure of a truncated form of cytochrome f from Chlamydomonas reinhardtii."
Chi Y.-I., Huang L.-S., Zhang Z., Fernandez-Velasco J.G., Berry E.A.
Biochemistry 39:7689-7701(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
[8]"Interruption of the internal water chain of cytochrome f impairs photosynthetic function."
Sainz G., Carrell C.J., Ponamarev M.V., Soriano G.M., Cramer W.A., Smith J.L.
Biochemistry 39:9164-9173(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X72917 Genomic DNA. Translation: CAA51422.1.
D01036 Genomic DNA. Translation: BAA00844.1.
X57744 Genomic DNA. Translation: CAA40911.1.
FJ423446 Genomic DNA. Translation: ACJ50091.1.
BK000554 Genomic DNA. Translation: DAA00904.1.
PIRS16916.
RefSeqNP_958358.1. NC_005353.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1CFMX-ray2.00A/B/C32-282[»]
1E2VX-ray1.85A/B/C32-282[»]
1E2WX-ray1.60A/B32-282[»]
1E2ZX-ray2.50A/B/C32-282[»]
1EWHX-ray2.35A/B/C32-282[»]
1Q90X-ray3.10A32-317[»]
ProteinModelPortalP23577.
SMRP23577. Positions 32-317.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48404N.

Proteomic databases

PRIDEP23577.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsDAA00904; DAA00904; DAA00904.
GeneID2716989.
KEGGcre:ChreCp001.

Phylogenomic databases

KOK02634.
ProtClustDBCHL00037.

Enzyme and pathway databases

BioCycCHLAMY:CHRECP001-MONOMER.
MetaCyc:CHRECP001-MONOMER.

Family and domain databases

Gene3D1.20.5.700. 1 hit.
2.60.40.830. 2 hits.
HAMAPMF_00610. Cytb6_f_cytF.
InterProIPR024058. Cyt-f_TM.
IPR002325. Cyt_f.
IPR024094. Cyt_f_lg_dom.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01333. Apocytochr_F_C. 1 hit.
[Graphical view]
PRINTSPR00610. CYTOCHROMEF.
SUPFAMSSF103431. SSF103431. 1 hit.
SSF49441. SSF49441. 2 hits.
SSF51246. SSF51246. 1 hit.
PROSITEPS51010. CYTF. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23577.

Entry information

Entry nameCYF_CHLRE
AccessionPrimary (citable) accession number: P23577
Secondary accession number(s): B7U1E4
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references