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P23577

- CYF_CHLRE

UniProt

P23577 - CYF_CHLRE

Protein

Apocytochrome f

Gene

petA

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions.

    Cofactori

    Binds 1 heme group covalently.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi32 – 321Iron (heme axial ligand); via amino nitrogen
    Binding sitei52 – 521Heme (covalent)
    Binding sitei55 – 551Heme (covalent)
    Metal bindingi56 – 561Iron (heme axial ligand)

    GO - Molecular functioni

    1. electron carrier activity Source: UniProtKB-HAMAP
    2. heme binding Source: InterPro
    3. iron ion binding Source: InterPro

    GO - Biological processi

    1. oxidation-reduction process Source: UniProtKB-HAMAP
    2. photosynthesis Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Electron transport, Photosynthesis, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciCHLAMY:CHRECP001-MONOMER.
    MetaCyc:CHRECP001-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Apocytochrome f
    Gene namesi
    Name:petA
    Encoded oniPlastid; Chloroplast
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
    ProteomesiUP000006906: Chloroplast

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast thylakoid membrane Source: UniProtKB-SubCell
    2. integral component of thylakoid membrane Source: InterPro

    Keywords - Cellular componenti

    Chloroplast, Membrane, Plastid, Thylakoid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 31311 PublicationAdd
    BLAST
    Chaini32 – 317286Apocytochrome fPRO_0000023809Add
    BLAST

    Proteomic databases

    PRIDEiP23577.

    Interactioni

    Subunit structurei

    The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer.

    Protein-protein interaction databases

    DIPiDIP-48404N.

    Structurei

    Secondary structure

    1
    317
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi33 – 397
    Beta strandi41 – 444
    Helixi51 – 544
    Beta strandi63 – 653
    Beta strandi68 – 703
    Beta strandi75 – 817
    Beta strandi86 – 883
    Beta strandi95 – 984
    Beta strandi101 – 1077
    Helixi117 – 1193
    Helixi122 – 1287
    Beta strandi134 – 1374
    Beta strandi143 – 1508
    Helixi151 – 1544
    Beta strandi155 – 1628
    Turni166 – 1683
    Beta strandi174 – 18613
    Beta strandi198 – 2003
    Beta strandi208 – 22013
    Beta strandi223 – 2286
    Beta strandi230 – 2323
    Beta strandi234 – 2396
    Beta strandi241 – 2433
    Beta strandi258 – 2603
    Beta strandi267 – 27711
    Helixi280 – 31435

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CFMX-ray2.00A/B/C32-282[»]
    1E2VX-ray1.85A/B/C32-282[»]
    1E2WX-ray1.60A/B32-282[»]
    1E2ZX-ray2.50A/B/C32-282[»]
    1EWHX-ray2.35A/B/C32-282[»]
    1Q90X-ray3.10A32-317[»]
    ProteinModelPortaliP23577.
    SMRiP23577. Positions 32-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23577.

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei283 – 30220HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome f family.Curated

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    KOiK02634.

    Family and domain databases

    Gene3Di1.20.5.700. 1 hit.
    2.60.40.830. 2 hits.
    HAMAPiMF_00610. Cytb6_f_cytF.
    InterProiIPR024058. Cyt-f_TM.
    IPR002325. Cyt_f.
    IPR024094. Cyt_f_lg_dom.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF01333. Apocytochr_F_C. 1 hit.
    [Graphical view]
    PRINTSiPR00610. CYTOCHROMEF.
    SUPFAMiSSF103431. SSF103431. 1 hit.
    SSF49441. SSF49441. 2 hits.
    SSF51246. SSF51246. 1 hit.
    PROSITEiPS51010. CYTF. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23577-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNQVFTTLR AATLAVILGM AGGLAVSPAQ AYPVFAQQNY ANPREANGRI    50
    VCANCHLAQK AVEIEVPQAV LPDTVFEAVI ELPYDKQVKQ VLANGKKGDL 100
    NVGMVLILPE GFELAPPDRV PAEIKEKVGN LYYQPYSPEQ KNILVVGPVP 150
    GKKYSEMVVP ILSPDPAKNK NVSYLKYPIY FGGNRGRGQV YPDGKKSNNT 200
    IYNASAAGKI VAITALSEKK GGFEVSIEKA NGEVVVDKIP AGPDLIVKEG 250
    QTVQADQPLT NNPNVGGFGQ AETEIVLQNP ARIQGLLVFF SFVLLTQVLL 300
    VLKKKQFEKV QLAEMNF 317
    Length:317
    Mass (Da):34,292
    Last modified:November 1, 1991 - v1
    Checksum:i1DC74E50552B7A53
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti53 – 531A → N AA sequence (PubMed:7493968)Curated
    Sequence conflicti64 – 641I → S in CAA40911. (PubMed:1868213)Curated
    Sequence conflicti89 – 913KQV → NS in CAA40911. (PubMed:1868213)Curated
    Sequence conflicti132 – 1321Y → F in CAA40911. (PubMed:1868213)Curated
    Sequence conflicti165 – 1651D → Y(PubMed:2060646)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72917 Genomic DNA. Translation: CAA51422.1.
    D01036 Genomic DNA. Translation: BAA00844.1.
    X57744 Genomic DNA. Translation: CAA40911.1.
    FJ423446 Genomic DNA. Translation: ACJ50091.1.
    BK000554 Genomic DNA. Translation: DAA00904.1.
    PIRiS16916.
    RefSeqiNP_958358.1. NC_005353.1.

    Genome annotation databases

    EnsemblPlantsiDAA00904; DAA00904; DAA00904.
    GeneIDi2716989.
    KEGGicre:ChreCp001.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X72917 Genomic DNA. Translation: CAA51422.1 .
    D01036 Genomic DNA. Translation: BAA00844.1 .
    X57744 Genomic DNA. Translation: CAA40911.1 .
    FJ423446 Genomic DNA. Translation: ACJ50091.1 .
    BK000554 Genomic DNA. Translation: DAA00904.1 .
    PIRi S16916.
    RefSeqi NP_958358.1. NC_005353.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CFM X-ray 2.00 A/B/C 32-282 [» ]
    1E2V X-ray 1.85 A/B/C 32-282 [» ]
    1E2W X-ray 1.60 A/B 32-282 [» ]
    1E2Z X-ray 2.50 A/B/C 32-282 [» ]
    1EWH X-ray 2.35 A/B/C 32-282 [» ]
    1Q90 X-ray 3.10 A 32-317 [» ]
    ProteinModelPortali P23577.
    SMRi P23577. Positions 32-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48404N.

    Proteomic databases

    PRIDEi P23577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi DAA00904 ; DAA00904 ; DAA00904 .
    GeneIDi 2716989.
    KEGGi cre:ChreCp001.

    Phylogenomic databases

    KOi K02634.

    Enzyme and pathway databases

    BioCyci CHLAMY:CHRECP001-MONOMER.
    MetaCyc:CHRECP001-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P23577.

    Family and domain databases

    Gene3Di 1.20.5.700. 1 hit.
    2.60.40.830. 2 hits.
    HAMAPi MF_00610. Cytb6_f_cytF.
    InterProi IPR024058. Cyt-f_TM.
    IPR002325. Cyt_f.
    IPR024094. Cyt_f_lg_dom.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF01333. Apocytochr_F_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00610. CYTOCHROMEF.
    SUPFAMi SSF103431. SSF103431. 1 hit.
    SSF49441. SSF49441. 2 hits.
    SSF51246. SSF51246. 1 hit.
    PROSITEi PS51010. CYTF. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequences of the continuous and separated petA, petB and petD chloroplast genes in Chlamydomonas reinhardtii."
      Bueschlen S., Choquet Y., Kuras R., Wollman F.A.
      FEBS Lett. 284:257-262(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 137c / CC-125.
    2. "Structural analysis and expression during dark-light transitions of a gene for cytochrome f in Chlamydomonas reinhardtii."
      Matsumoto T., Matsuo M., Matsuda Y.
      Plant Cell Physiol. 32:863-872(1991)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cw15.
    3. "Nucleotide sequence of the petA (cytochrome f) gene from the green alga, Chlamydomonas reinhardtii."
      Bertsch J., Malkin R.
      Plant Mol. Biol. 17:131-133(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis."
      Smith D.R., Lee R.W.
      BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CC-503.
    5. "Purification and characterization of the cytochrome b6 f complex from Chlamydomonas reinhardtii."
      Pierre Y., Breyton C., Kramer D., Popot J.-L.
      J. Biol. Chem. 270:29342-29349(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 32-53, CHARACTERIZATION.
      Strain: WT12.
    6. "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats."
      Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B.
      Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
    7. "X-ray structure of a truncated form of cytochrome f from Chlamydomonas reinhardtii."
      Chi Y.-I., Huang L.-S., Zhang Z., Fernandez-Velasco J.G., Berry E.A.
      Biochemistry 39:7689-7701(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
    8. "Interruption of the internal water chain of cytochrome f impairs photosynthetic function."
      Sainz G., Carrell C.J., Ponamarev M.V., Soriano G.M., Cramer W.A., Smith J.L.
      Biochemistry 39:9164-9173(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS).

    Entry informationi

    Entry nameiCYF_CHLRE
    AccessioniPrimary (citable) accession number: P23577
    Secondary accession number(s): B7U1E4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 116 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3