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P23572

- CDK1_DROME

UniProt

P23572 - CDK1_DROME

Protein

Cyclin-dependent kinase 1

Gene

cdc2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 158 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.
    ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-161 activates it.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei128 – 1281Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: FlyBase
    3. protein binding Source: UniProtKB
    4. protein kinase activity Source: FlyBase
    5. protein serine/threonine kinase activity Source: FlyBase
    6. RNA polymerase II carboxy-terminal domain kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. asymmetric neuroblast division Source: FlyBase
    2. cellular process Source: FlyBase
    3. cellular response to DNA damage stimulus Source: FlyBase
    4. G1/S transition of mitotic cell cycle Source: FlyBase
    5. G2/M transition of mitotic cell cycle Source: FlyBase
    6. germarium-derived cystoblast division Source: FlyBase
    7. male meiosis Source: FlyBase
    8. male meiosis I Source: FlyBase
    9. meiotic G2/MI transition Source: FlyBase
    10. mitotic cell cycle Source: FlyBase
    11. mitotic G2/M transition checkpoint Source: FlyBase
    12. mitotic G2 DNA damage checkpoint Source: FlyBase
    13. mitotic nuclear division Source: UniProtKB-KW
    14. neurogenesis Source: FlyBase
    15. protein phosphorylation Source: FlyBase
    16. regulation of protein localization Source: FlyBase
    17. spermatogenesis Source: FlyBase
    18. spermatogonial cell division Source: FlyBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_180285. G1/S-Specific Transcription.
    REACT_184285. Condensation of Prometaphase Chromosomes.
    REACT_184304. Nuclear Pore Complex (NPC) Disassembly.
    REACT_184356. MASTL Facilitates Mitotic Progression.
    REACT_209388. E2F mediated regulation of DNA replication.
    REACT_219169. Depolymerisation of the Nuclear Lamina.
    REACT_220914. ERK1 activation.
    REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
    SignaLinkiP23572.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 1 (EC:2.7.11.22, EC:2.7.11.23)
    Short name:
    CDK1
    Alternative name(s):
    Cell division control protein 2 homolog
    Cell division protein kinase 1
    p34 protein kinase
    Gene namesi
    Name:cdc2
    Synonyms:cdk1
    ORF Names:CG5363
    OrganismiDrosophila melanogaster (Fruit fly)
    Taxonomic identifieri7227 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
    ProteomesiUP000000803: Chromosome 2L

    Organism-specific databases

    FlyBaseiFBgn0004106. cdc2.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. microtubule associated complex Source: FlyBase
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi43 – 431G → D in cdc2-E1-4; larval-pupal lethal with some adult escapers. 1 Publication
    Mutagenesisi145 – 1451A → V in cdc2-216A; larval-pupal lethal. 2 Publications
    Mutagenesisi148 – 1481G → R in cdc2-D57; embryonic or larval-pupal lethal. 2 Publications
    Mutagenesisi176 – 1761L → Q in cdc2-E10; larval-pupal lethal. 1 Publication
    Mutagenesisi196 – 1961E → K in cdc2-E1-24; larval-pupal lethal with some adult escapers. 2 Publications
    Mutagenesisi206 – 2061G → D in cdc2-E1-23; larval-pupal lethal. 2 Publications
    Mutagenesisi242 – 2421P → S in cdc2-E1-9; larval-pupal lethal. 2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Cyclin-dependent kinase 1PRO_0000085743Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141Phosphothreonine1 Publication
    Modified residuei15 – 151Phosphotyrosine1 Publication
    Modified residuei160 – 1601Phosphotyrosine1 Publication
    Modified residuei161 – 1611Phosphothreonine; by CAKSequence Analysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP23572.
    PRIDEiP23572.

    Expressioni

    Developmental stagei

    Expressed both maternally and zygotically.2 Publications

    Gene expression databases

    BgeeiP23572.

    Interactioni

    Subunit structurei

    Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cks85AQ9VHN14EBI-108689,EBI-122930
    CycB3Q9I7I02EBI-108689,EBI-150964
    CycCP250084EBI-108689,EBI-195485
    CycEP547336EBI-108689,EBI-203549
    CycKQ961D14EBI-108689,EBI-130995
    tweQ030194EBI-108689,EBI-138996

    Protein-protein interaction databases

    BioGridi60495. 38 interactions.
    DIPiDIP-649N.
    IntActiP23572. 25 interactions.
    MINTiMINT-292976.

    Structurei

    3D structure databases

    ProteinModelPortaliP23572.
    SMRiP23572. Positions 1-287.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 287284Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00720000108415.
    InParanoidiP23572.
    KOiK02087.
    OMAiDILEHPY.
    OrthoDBiEOG7966H8.
    PhylomeDBiP23572.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23572-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDFEKIEKI GEGTYGVVYK GRNRLTGQIV AMKKIRLESD DEGVPSTAIR    50
    EISLLKELKH ENIVCLEDVL MEENRIYLIF EFLSMDLKKY MDSLPVDKHM 100
    ESELVRSYLY QITSAILFCH RRRVLHRDLK PQNLLIDKSG LIKVADFGLG 150
    RSFGIPVRIY THEIVTLWYR APEVLLGSPR YSCPVDIWSI GCIFAEMATR 200
    KPLFQGDSEI DQLFRMFRIL KTPTEDIWPG VTSLPDYKNT FPCWSTNQLT 250
    NQLKNLDANG IDLIQKMLIY DPVHRISAKD ILEHPYFNGF QSGLVRN 297
    Length:297
    Mass (Da):34,439
    Last modified:November 1, 1991 - v1
    Checksum:i952CF136D8AB64C0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57485 mRNA. Translation: CAA40723.1.
    X57496 mRNA. Translation: CAA40733.1.
    S66801 Genomic DNA. Translation: AAP13986.1.
    S66804 Genomic DNA. Translation: AAP13987.1.
    S66805 Genomic DNA. Translation: AAP13988.1.
    S66807 Genomic DNA. Translation: AAP13989.1.
    S66810 Genomic DNA. Translation: AAP13990.1.
    AM294319 Genomic DNA. Translation: CAL26249.1.
    AM294320 Genomic DNA. Translation: CAL26250.1.
    AM294321 Genomic DNA. Translation: CAL26251.1.
    AM294322 Genomic DNA. Translation: CAL26252.1.
    AM294323 Genomic DNA. Translation: CAL26253.1.
    AM294324 Genomic DNA. Translation: CAL26254.1.
    AM294325 Genomic DNA. Translation: CAL26255.1.
    AM294326 Genomic DNA. Translation: CAL26256.1.
    AM294327 Genomic DNA. Translation: CAL26257.1.
    AM294328 Genomic DNA. Translation: CAL26258.1.
    AM294329 Genomic DNA. Translation: CAL26259.1.
    AE014134 Genomic DNA. Translation: AAF52932.1.
    AY061450 mRNA. Translation: AAL28998.1.
    PIRiS12009.
    RefSeqiNP_476797.1. NM_057449.4.
    UniGeneiDm.3187.

    Genome annotation databases

    EnsemblMetazoaiFBtr0080051; FBpp0079641; FBgn0004106.
    GeneIDi34411.
    KEGGidme:Dmel_CG5363.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X57485 mRNA. Translation: CAA40723.1 .
    X57496 mRNA. Translation: CAA40733.1 .
    S66801 Genomic DNA. Translation: AAP13986.1 .
    S66804 Genomic DNA. Translation: AAP13987.1 .
    S66805 Genomic DNA. Translation: AAP13988.1 .
    S66807 Genomic DNA. Translation: AAP13989.1 .
    S66810 Genomic DNA. Translation: AAP13990.1 .
    AM294319 Genomic DNA. Translation: CAL26249.1 .
    AM294320 Genomic DNA. Translation: CAL26250.1 .
    AM294321 Genomic DNA. Translation: CAL26251.1 .
    AM294322 Genomic DNA. Translation: CAL26252.1 .
    AM294323 Genomic DNA. Translation: CAL26253.1 .
    AM294324 Genomic DNA. Translation: CAL26254.1 .
    AM294325 Genomic DNA. Translation: CAL26255.1 .
    AM294326 Genomic DNA. Translation: CAL26256.1 .
    AM294327 Genomic DNA. Translation: CAL26257.1 .
    AM294328 Genomic DNA. Translation: CAL26258.1 .
    AM294329 Genomic DNA. Translation: CAL26259.1 .
    AE014134 Genomic DNA. Translation: AAF52932.1 .
    AY061450 mRNA. Translation: AAL28998.1 .
    PIRi S12009.
    RefSeqi NP_476797.1. NM_057449.4.
    UniGenei Dm.3187.

    3D structure databases

    ProteinModelPortali P23572.
    SMRi P23572. Positions 1-287.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 60495. 38 interactions.
    DIPi DIP-649N.
    IntActi P23572. 25 interactions.
    MINTi MINT-292976.

    Proteomic databases

    PaxDbi P23572.
    PRIDEi P23572.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai FBtr0080051 ; FBpp0079641 ; FBgn0004106 .
    GeneIDi 34411.
    KEGGi dme:Dmel_CG5363.

    Organism-specific databases

    CTDi 34411.
    FlyBasei FBgn0004106. cdc2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00720000108415.
    InParanoidi P23572.
    KOi K02087.
    OMAi DILEHPY.
    OrthoDBi EOG7966H8.
    PhylomeDBi P23572.

    Enzyme and pathway databases

    Reactomei REACT_180285. G1/S-Specific Transcription.
    REACT_184285. Condensation of Prometaphase Chromosomes.
    REACT_184304. Nuclear Pore Complex (NPC) Disassembly.
    REACT_184356. MASTL Facilitates Mitotic Progression.
    REACT_209388. E2F mediated regulation of DNA replication.
    REACT_219169. Depolymerisation of the Nuclear Lamina.
    REACT_220914. ERK1 activation.
    REACT_222733. E2F-enabled inhibition of pre-replication complex formation.
    REACT_224351. Regulation of APC/C activators between G1/S and early anaphase.
    REACT_225636. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.
    REACT_33104. APC/C:Cdc20 mediated degradation of Cyclin B.
    SignaLinki P23572.

    Miscellaneous databases

    GenomeRNAii 34411.
    NextBioi 788369.
    PROi P23572.

    Gene expression databases

    Bgeei P23572.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Drosophila cdc2 homologs: a functional homolog is coexpressed with a cognate variant."
      Lehner C.F., O'Farrell P.H.
      EMBO J. 9:3573-3581(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Complementation of fission yeast cdc2ts and cdc25ts mutants identifies two cell cycle genes from Drosophila: a cdc2 homologue and string."
      Jimenez J., Alphey L., Nurse P., Glover D.M.
      EMBO J. 9:3565-3571(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    3. "Genetic analysis of the Drosophila cdc2 homolog."
      Stern B., Ried G., Clegg N.J., Grigliatti T.A., Lehner C.F.
      Development 117:219-232(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], MUTAGENESIS OF ALA-145; GLY-148; GLU-196; GLY-206 AND PRO-242.
    4. "Widespread adaptive evolution of Drosophila genes with sex-biased expression."
      Proeschel M., Zhang Z., Parsch J.
      Genetics 174:893-900(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL229, ZBMEL377, ZBMEL384, ZBMEL398, ZBMEL82, ZBMEL84 and ZBMEL95.
    5. "The genome sequence of Drosophila melanogaster."
      Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
      , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
      Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Berkeley.
    6. Cited for: GENOME REANNOTATION.
      Strain: Berkeley.
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Berkeley.
    8. "A developmental and molecular analysis of cdc2 mutations in Drosophila melanogaster."
      Clegg N.J., Whitehead I.P., Williams J.A., Spiegelman G.B., Grigliatti T.A.
      Genome 36:676-685(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF GLY-43; ALA-145; GLY-148; LEU-176; GLU-196; GLY-206 AND PRO-242, DEVELOPMENTAL STAGE.
    9. "Phosphoproteome analysis of Drosophila melanogaster embryos."
      Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
      J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-14; TYR-15; TYR-160 AND THR-161, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryo.

    Entry informationi

    Entry nameiCDK1_DROME
    AccessioniPrimary (citable) accession number: P23572
    Secondary accession number(s): A0ANT1
    , Q86FT5, Q86FT6, Q86FT7, Q86FT8, Q86FT9, Q9TX68, Q9TX69, Q9TX70, Q9TX71, Q9TX72, Q9TX73, Q9TX74, Q9VKX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 158 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programDrosophila annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Drosophila
      Drosophila: entries, gene names and cross-references to FlyBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3