P23571 (3MG_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA-3-methyladenine glycosylase
EC=3.2.2.21

Alternative name(s):
3-alkyladenine DNA glycosylase
3-methyladenine DNA glycosidase
ADPG
N-methylpurine-DNA glycosylase

Gene names

Name:

Mpg

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	317 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine, and 7-methylguanine from the damaged DNA polymer formed by alkylation lesions.
Catalytic activity	Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.
Subunit structure	Binds MBD1 By similarity.
Subcellular location	Nucleus Potential.
Sequence similarities	Belongs to the DNA glycosylase MPG family.
Sequence caution	The sequence CAA39814.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	DNA damage DNA repair
Cellular component	Nucleus
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-3-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-3-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methyladenine glycosylase activity Inferred from electronic annotation. Source: EC DNA-7-methylguanine glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 317	›317	DNA-3-methyladenine glycosylase		PRO_0000100067
Amino acid modifications
	Modified residue	84	1	Phosphoserine By similarity
	Modified residue	258	1	Phosphoserine By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P23571 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: 613849E4ADEF1BDB	FASTA	317	34,814
	10 20 30 40 50 60 SKEPVSVVLP DAEHPAFPGR TRRPGNARAG SQVTGSREVG QMPAPLSRKI GQKKQQLAQS 70 80 90 100 110 120 EQQQTPKEKL SSTPGLLRSI YFSSPEDRPA RLGPEYFDQP AVTLARAFLG QVLVRRLADG 130 140 150 160 170 180 TELRGRIVET EAYLGPEDEA AHSRGGRQTP RNRGMFMKPG TLYVYLIYGM YFCLNVSSQG 190 200 210 220 230 240 AGACVLLRAL EPLEGLETMR QLRNSLRKST VGRSLKDREL CNGPSKLCQA LARSKSFDQR 250 260 270 280 290 300 DLAQDEAVWL EHGPLESSSP AVVAAAAGIG HAGEWTQKPL RFYVQGSPWV SVVDRVAEQM 310 YQPQQTACSD XALIVQK

References

[1]	"Isolation and structure of a cDNA expressing a mammalian 3-methyladenine-DNA glycosylase." O'Connor T.R., Laval F. EMBO J. 9:3337-3342(1990) [PubMed: 1698614] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56420 mRNA. Translation: CAA39814.1. Frameshift.
IPI	IPI00958080.
PIR	S12059.
UniGene	Rn.11241.
3D structure databases
ProteinModelPortal	P23571.
SMR	P23571. Positions 91-299.
ModBase	Search...
Protein-protein interaction databases
STRING	P23571.
Proteomic databases
PRIDE	P23571.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	3106. Mpg.
Phylogenomic databases
eggNOG	roNOG09926.
GeneTree	ENSGT00390000009825.
HOVERGEN	HBG000019.
InParanoid	P23571.
OrthoDB	EOG4KD6N3.
Gene expression databases
ArrayExpress	P23571.
Genevestigator	P23571.
GermOnline	ENSRNOG00000020571. Rattus norvegicus.
Family and domain databases
InterPro	IPR011034. Formyl_transferase_C-like. IPR003180. PurDNA_glycsylse. [Graphical view]
Gene3D	G3DSA:3.10.300.10. PurDNA_glycsylse. 1 hit.
PANTHER	PTHR10429. PurDNA_glycsylse. 1 hit.
Pfam	PF02245. Pur_DNA_glyco. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit.
TIGRFAMs	TIGR00567. 3mg. 1 hit.
ProtoNet	Search...

Entry information

Entry name

3MG_RAT

Accession

Primary (citable) accession number: P23571

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program