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P23569 (CHSY_PUEML) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Chalcone synthase

EC=2.3.1.74
Alternative name(s):
Naringenin-chalcone synthase
Gene names
Name:CHS
OrganismPueraria montana var. lobata (Kudzu vine) (Pueraria lobata)
Taxonomic identifier3893 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaePueraria

Protein attributes

Sequence length389 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The primary product of this enzyme is 4,2',4',6'-tetrahydroxychalcone (also termed naringenin-chalcone or chalcone) which can under specific conditions spontaneously isomerize into naringenin.

Catalytic activity

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO2.

Pathway

Secondary metabolite biosynthesis; flavonoid biosynthesis.

Sequence similarities

Belongs to the chalcone/stilbene synthases family.

Ontologies

Keywords
   Biological processFlavonoid biosynthesis
   Molecular functionAcyltransferase
Transferase
Gene Ontology (GO)
   Biological_processflavonoid biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Molecular_functionnaringenin-chalcone synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 389389Chalcone synthase
PRO_0000216043

Sites

Active site1641 By similarity

Sequences

Sequence LengthMass (Da)Tools
P23569 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 438F6DC77A37F5DE

FASTA38942,832
        10         20         30         40         50         60 
MVSVAEIRQA QRAEGPATIL AIGTANPPNC VDQSTYPDYY FRITNSEHMT ELKEKFQRMC 

        70         80         90        100        110        120 
DKSMIKKRYM YLTEEILKEN PNMCAYMAPS LDARQDMVVV EVPKLGKEAA TKAIKEWGQP 

       130        140        150        160        170        180 
KSKITHLIFC TTSGVDMPGA DYQLTKQLGL RPYVKRYMMY QQGCFAGGTV LRLAKDLAEN 

       190        200        210        220        230        240 
NKGARVLVVC SEITAVTFRG PSDTHLDSLV GQALFGDGAA AVIVGSDPIP QVEKPLYELV 

       250        260        270        280        290        300 
WTAQTIAPDS EGAIDGHLRE VGLTFHLLKD VPGIVSKNID KALFEAFNPL NISDYNSIFW 

       310        320        330        340        350        360 
IAHPGGPAIL DQVEQKLGLK PEKMKATRDV LSDYGNMSSA CVLFILDEMR RKSAENGLKT 

       370        380 
TGEGLEWGVL FGFGPGLTIE TVVLRSVAI 

« Hide

References

[1]"Isolation, sequence and bacterial expression of a cDNA for chalcone synthase from the cultured cells of Pueraria lobata."
Nakajima O., Akiyama T., Hakamatsuka T., Shibuya M., Noguchi H., Ebizuka Y., Sankawa U.
Chem. Pharm. Bull. 39:1911-1913(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"cDNA and genomic DNA clonings of chalcone synthase from Pueraria lobata."
Nakajima O., Shibuya M., Hakamatsuka T., Noguchi H., Ebizuka Y., Sankawa U.
Biol. Pharm. Bull. 19:71-76(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-59.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10223 mRNA. Translation: BAA01075.1.
D63855 Genomic DNA. Translation: BAA09918.1.
PIRSYFJCP. JQ0911.

3D structure databases

ProteinModelPortalP23569.
SMRP23569. Positions 3-389.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

ProMEXP23569.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00154.

Family and domain databases

Gene3D3.40.47.10. 2 hits.
InterProIPR012328. Chalcone/stilbene_synth_C.
IPR018088. Chalcone/stilbene_synthase_AS.
IPR001099. Chalcone/stilbene_synthase_N.
IPR011141. Polyketide_synthase_type-III.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamPF02797. Chal_sti_synt_C. 1 hit.
PF00195. Chal_sti_synt_N. 1 hit.
[Graphical view]
PIRSFPIRSF000451. PKS_III. 1 hit.
SUPFAMSSF53901. SSF53901. 2 hits.
PROSITEPS00441. CHALCONE_SYNTH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCHSY_PUEML
AccessionPrimary (citable) accession number: P23569
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways