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P23566

- UBC2_SCHPO

UniProt

P23566 - UBC2_SCHPO

Protein

Ubiquitin-conjugating enzyme E2 2

Gene

rhp6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 3 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins. Component of the histone H2B ubiquitin ligase complex (HULC) which plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation By similarity. Required for obr1 ubiquitination, which regulates mating-type silencing. With cut8, regulates the nuclear accumulation of the proteasome.6 PublicationsPROSITE-ProRule annotation

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediate

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. protein binding Source: PomBase
    4. ubiquitin-protein transferase activity Source: PomBase

    GO - Biological processi

    1. cellular response to hyperoxia Source: PomBase
    2. chromatin assembly Source: PomBase
    3. chromatin remodeling Source: PomBase
    4. chromatin silencing Source: PomBase
    5. chromatin silencing at silent mating-type cassette Source: PomBase
    6. DNA repair Source: UniProtKB-KW
    7. DNA replication-independent nucleosome assembly Source: PomBase
    8. histone H2B conserved C-terminal lysine ubiquitination Source: PomBase
    9. negative regulation of SREBP signaling pathway by positive regulation of transcription factor catabolic process in response to increased oxygen levels Source: PomBase
    10. negative regulation of transcription by transcription factor catabolism Source: PomBase
    11. proteasome localization Source: PomBase
    12. proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
    13. regulation of histone H3-K4 methylation Source: PomBase
    14. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
    15. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Chromatin regulator, Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation, Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
    Alternative name(s):
    RAD6 homolog
    Ubiquitin carrier protein 2
    Ubiquitin-protein ligase 2
    Gene namesi
    Name:rhp6
    Synonyms:ubc2
    ORF Names:SPAC18B11.07c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC18B11.07c.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. HULC complex Source: PomBase
    3. nuclear chromatin Source: PomBase
    4. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi88 – 881C → A: No derepression of transcription. 1 Publication
    Mutagenesisi88 – 881C → S: No derepression of transcription. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 151151Ubiquitin-conjugating enzyme E2 2PRO_0000082536Add
    BLAST

    Proteomic databases

    MaxQBiP23566.
    PaxDbiP23566.

    Interactioni

    Subunit structurei

    Component of the histone H2B ubiquitin ligase complex (HULC) composed of at least brl1, brl2, rhp6 and shf1.1 Publication

    Protein-protein interaction databases

    BioGridi279076. 10 interactions.
    IntActiP23566. 4 interactions.
    MINTiMINT-4687768.
    STRINGi4896.SPAC18B11.07c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP23566.
    SMRiP23566. Positions 2-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233454.
    KOiK10573.
    OMAiPVPDNVM.
    OrthoDBiEOG7SBP18.
    PhylomeDBiP23566.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSTTARRRLM RDFKRMQQDP PAGVSASPVS DNVMLWNAVI IGPADTPFED    50
    GTFKLVLSFD EQYPNKPPLV KFVSTMFHPN VYANGELCLD ILQNRWSPTY 100
    DVAAILTSIQ SLLNDPNNAS PANAEAAQLH RENKKEYVRR VRKTVEDSWE 150
    S 151
    Length:151
    Mass (Da):17,097
    Last modified:November 1, 1995 - v3
    Checksum:iE4A88A40ECF35709
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti111 – 1111S → R in CAA37340. (PubMed:2184030)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53252 Genomic DNA. Translation: CAA37340.1.
    CU329670 Genomic DNA. Translation: CAA90592.1.
    PIRiS12529.
    T45220.
    RefSeqiNP_592876.1. NM_001018276.2.

    Genome annotation databases

    EnsemblFungiiSPAC18B11.07c.1; SPAC18B11.07c.1:pep; SPAC18B11.07c.
    GeneIDi2542622.
    KEGGispo:SPAC18B11.07c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53252 Genomic DNA. Translation: CAA37340.1 .
    CU329670 Genomic DNA. Translation: CAA90592.1 .
    PIRi S12529.
    T45220.
    RefSeqi NP_592876.1. NM_001018276.2.

    3D structure databases

    ProteinModelPortali P23566.
    SMRi P23566. Positions 2-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 279076. 10 interactions.
    IntActi P23566. 4 interactions.
    MINTi MINT-4687768.
    STRINGi 4896.SPAC18B11.07c-1.

    Proteomic databases

    MaxQBi P23566.
    PaxDbi P23566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC18B11.07c.1 ; SPAC18B11.07c.1:pep ; SPAC18B11.07c .
    GeneIDi 2542622.
    KEGGi spo:SPAC18B11.07c.

    Organism-specific databases

    PomBasei SPAC18B11.07c.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233454.
    KOi K10573.
    OMAi PVPDNVM.
    OrthoDBi EOG7SBP18.
    PhylomeDBi P23566.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_188524. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    NextBioi 20803671.
    PROi P23566.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The rhp6+ gene of Schizosaccharomyces pombe: a structural and functional homolog of the RAD6 gene from the distantly related yeast Saccharomyces cerevisiae."
      Reynolds P., Koken M.H.M., Hoeijmakers J.H.J., Prakash S., Prakash L.
      EMBO J. 9:1423-1430(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    3. "A novel function of the DNA repair gene rhp6 in mating-type silencing by chromatin remodeling in fission yeast."
      Singh J., Goel V., Klar A.J.S.
      Mol. Cell. Biol. 18:5511-5522(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    4. "The fission yeast ubiquitin-conjugating enzymes UbcP3, Ubc15, and Rhp6 affect transcriptional silencing of the mating-type region."
      Nielsen I.S., Nielsen O., Murray J.M., Thon G.
      Eukaryot. Cell 1:613-625(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF CYS-88.
    5. "Two ubiquitin-conjugating enzymes, Rhp6 and UbcX, regulate heterochromatin silencing in Schizosaccharomyces pombe."
      Choi E.S., Kim H.S., Jang Y.K., Hong S.H., Park S.D.
      Mol. Cell. Biol. 22:8366-8374(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Identification of Uhp1, a ubiquitinated histone-like protein, as a target/mediator of Rhp6 in mating-type silencing in fission yeast."
      Naresh A., Saini S., Singh J.
      J. Biol. Chem. 278:9185-9194(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "Regulation of nuclear proteasome by Rhp6/Ubc2 through ubiquitination and destruction of the sensor and anchor Cut8."
      Takeda K., Yanagida M.
      Cell 122:393-405(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    8. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
      Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
      Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. "HULC, a histone H2B ubiquitinating complex, modulates heterochromatin independent of histone methylation in fission yeast."
      Zofall M., Grewal S.I.S.
      J. Biol. Chem. 282:14065-14072(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE HULC COMPLEX, FUNCTION OF THE HULC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.

    Entry informationi

    Entry nameiUBC2_SCHPO
    AccessioniPrimary (citable) accession number: P23566
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 124 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3