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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

rhp6

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Component of the histone H2B ubiquitin ligase complex (HULC) which plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation (By similarity). Required for obr1 ubiquitination, which regulates mating-type silencing. With cut8, regulates the nuclear accumulation of the proteasome.PROSITE-ProRule annotation6 Publications

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediate

GO - Molecular functioni

GO - Biological processi

  • cellular response to hyperoxia Source: PomBase
  • chromatin assembly Source: PomBase
  • chromatin organization Source: PomBase
  • DNA repair Source: GO_Central
  • histone H2B conserved C-terminal lysine ubiquitination Source: PomBase
  • negative regulation of chromatin silencing at silent mating-type cassette Source: PomBase
  • negative regulation of chromatin silencing at telomere Source: PomBase
  • negative regulation of SREBP signaling pathway by positive regulation of transcription factor catabolic process in response to increased oxygen levels Source: PomBase
  • negative regulation of transcription by transcription factor catabolism Source: PomBase
  • proteasome localization to nuclear periphery Source: PomBase
  • proteasome-mediated ubiquitin-dependent protein catabolic process Source: PomBase
  • protein polyubiquitination involved in nucleus-associated proteasomal ubiquitin-dependent protein catabolic process Source: PomBase
  • regulation of histone H3-K4 methylation Source: PomBase
  • sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Transferase

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 2
RAD6 homolog
Ubiquitin carrier protein 2
Ubiquitin-protein ligase 2
Gene namesi
Name:rhp6
Synonyms:ubc2
ORF Names:SPAC18B11.07c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC18B11.07c.
PomBaseiSPAC18B11.07c. rhp6.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
  • HULC complex Source: PomBase
  • nuclear chromatin Source: GO_Central
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi88 – 881C → A: No derepression of transcription. 1 Publication
Mutagenesisi88 – 881C → S: No derepression of transcription. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 151151Ubiquitin-conjugating enzyme E2 2PRO_0000082536Add
BLAST

Proteomic databases

MaxQBiP23566.

Interactioni

Subunit structurei

Component of the histone H2B ubiquitin ligase complex (HULC) composed of at least brl1, brl2, rhp6 and shf1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi279076. 12 interactions.
IntActiP23566. 4 interactions.
MINTiMINT-4687768.

Structurei

3D structure databases

ProteinModelPortaliP23566.
SMRiP23566. Positions 2-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000233454.
InParanoidiP23566.
KOiK10573.
OMAiNEYPNRP.
OrthoDBiEOG092C50OL.
PhylomeDBiP23566.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTTARRRLM RDFKRMQQDP PAGVSASPVS DNVMLWNAVI IGPADTPFED
60 70 80 90 100
GTFKLVLSFD EQYPNKPPLV KFVSTMFHPN VYANGELCLD ILQNRWSPTY
110 120 130 140 150
DVAAILTSIQ SLLNDPNNAS PANAEAAQLH RENKKEYVRR VRKTVEDSWE

S
Length:151
Mass (Da):17,097
Last modified:November 1, 1995 - v3
Checksum:iE4A88A40ECF35709
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111S → R in CAA37340 (PubMed:2184030).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53252 Genomic DNA. Translation: CAA37340.1.
CU329670 Genomic DNA. Translation: CAA90592.1.
PIRiS12529.
T45220.
RefSeqiNP_592876.1. NM_001018276.2.

Genome annotation databases

EnsemblFungiiSPAC18B11.07c.1; SPAC18B11.07c.1:pep; SPAC18B11.07c.
GeneIDi2542622.
KEGGispo:SPAC18B11.07c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53252 Genomic DNA. Translation: CAA37340.1.
CU329670 Genomic DNA. Translation: CAA90592.1.
PIRiS12529.
T45220.
RefSeqiNP_592876.1. NM_001018276.2.

3D structure databases

ProteinModelPortaliP23566.
SMRiP23566. Positions 2-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279076. 12 interactions.
IntActiP23566. 4 interactions.
MINTiMINT-4687768.

Proteomic databases

MaxQBiP23566.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC18B11.07c.1; SPAC18B11.07c.1:pep; SPAC18B11.07c.
GeneIDi2542622.
KEGGispo:SPAC18B11.07c.

Organism-specific databases

EuPathDBiFungiDB:SPAC18B11.07c.
PomBaseiSPAC18B11.07c. rhp6.

Phylogenomic databases

HOGENOMiHOG000233454.
InParanoidiP23566.
KOiK10573.
OMAiNEYPNRP.
OrthoDBiEOG092C50OL.
PhylomeDBiP23566.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-SPO-110314. Recognition of DNA damage by PCNA-containing replication complex.
R-SPO-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

PROiP23566.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC2_SCHPO
AccessioniPrimary (citable) accession number: P23566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 143 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.