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Protein

Alpha-internexin

Gene

Ina

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges (By similarity).By similarity

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD
  • structural molecule activity Source: InterPro

GO - Biological processi

  • cell differentiation Source: UniProtKB-KW
  • nervous system development Source: UniProtKB-KW
  • tissue regeneration Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Neurogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-internexin
Short name:
Alpha-Inx
Gene namesi
Name:Ina
Synonyms:Inexa
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2911. Ina.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 505505Alpha-internexinPRO_0000063785Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721PhosphoserineBy similarity
Modified residuei219 – 2191PhosphoserineBy similarity
Modified residuei290 – 2901N6-acetyllysineBy similarity
Modified residuei335 – 3351PhosphoserineCombined sources
Modified residuei474 – 4741PhosphoserineCombined sources
Modified residuei502 – 5021PhosphoserineBy similarity

Post-translational modificationi

O-glycosylated.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP23565.
PRIDEiP23565.

2D gel databases

World-2DPAGE0004:P23565.

PTM databases

iPTMnetiP23565.
PhosphoSiteiP23565.

Expressioni

Developmental stagei

Levels of this protein reach a maximum at embryonic day 16 and decline into adulthood.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
NsmfQ9EPI65EBI-6899875,EBI-6899705

GO - Molecular functioni

  • protein heterodimerization activity Source: RGD

Protein-protein interaction databases

BioGridi246662. 3 interactions.
IntActiP23565. 3 interactions.
MINTiMINT-4589517.
STRINGi10116.ENSRNOP00000027417.

Structurei

3D structure databases

ProteinModelPortaliP23565.
SMRiP23565. Positions 92-129, 325-402.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 8787HeadAdd
BLAST
Regioni88 – 408321RodAdd
BLAST
Regioni88 – 12942Coil 1AAdd
BLAST
Regioni130 – 14213Linker 1Add
BLAST
Regioni143 – 23896Coil 1BAdd
BLAST
Regioni239 – 26224Linker 2Add
BLAST
Regioni263 – 408146Coil 2Add
BLAST
Regioni409 – 50597TailAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi449 – 45810Poly-Glu

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP23565.
PhylomeDBiP23565.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23565-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFGSEHYLC SASSYRKVFG DGSRLSARLS GPGASGSFRS QSLSRSNVAS
60 70 80 90 100
TAACSSASSL GLGLAYRRLP ASDGLDLSQA AARTNEYKII RTNEKEQLQG
110 120 130 140 150
LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELREL
160 170 180 190 200
RAQLEEASSA RAQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ
210 220 230 240 250
RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA
260 270 280 290 300
EVDVAVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR
310 320 330 340 350
STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE
360 370 380 390 400
VAGYQDSIGQ LESDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL
410 420 430 440 450
EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSSTTSKVS SAGLSLKKEE
460 470 480 490 500
EEEEEEEEGA SKEVTKKTSK VGESFEETLE ETVVSTKKTE KSTIEEITTS

SSQKM
Length:505
Mass (Da):56,115
Last modified:October 1, 1996 - v2
Checksum:i287E548F0DA4D818
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti152 – 1532AQ → GE in CAA36264 (PubMed:2311576).Curated
Sequence conflicti239 – 2424Missing AA sequence (PubMed:2311576).Curated
Sequence conflicti458 – 4581Missing AA sequence (PubMed:2311576).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52017 mRNA. Translation: CAA36264.1.
M73049 Genomic DNA. Translation: AAA41444.1.
PIRiA41023.
UniGeneiRn.88941.

Genome annotation databases

UCSCiRGD:2911. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52017 mRNA. Translation: CAA36264.1.
M73049 Genomic DNA. Translation: AAA41444.1.
PIRiA41023.
UniGeneiRn.88941.

3D structure databases

ProteinModelPortaliP23565.
SMRiP23565. Positions 92-129, 325-402.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi246662. 3 interactions.
IntActiP23565. 3 interactions.
MINTiMINT-4589517.
STRINGi10116.ENSRNOP00000027417.

PTM databases

iPTMnetiP23565.
PhosphoSiteiP23565.

2D gel databases

World-2DPAGE0004:P23565.

Proteomic databases

PaxDbiP23565.
PRIDEiP23565.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:2911. rat.

Organism-specific databases

RGDi2911. Ina.

Phylogenomic databases

eggNOGiENOG410IGME. Eukaryota.
ENOG410XPTM. LUCA.
HOGENOMiHOG000230977.
HOVERGENiHBG013015.
InParanoidiP23565.
PhylomeDBiP23565.

Miscellaneous databases

PROiP23565.

Family and domain databases

InterProiIPR027703. Alpha-Inx.
IPR001664. IF.
IPR006821. Intermed_filament_DNA-bd.
IPR018039. Intermediate_filament_CS.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PTHR23239:SF132. PTHR23239:SF132. 1 hit.
PfamiPF00038. Filament. 1 hit.
PF04732. Filament_head. 1 hit.
[Graphical view]
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAINX_RAT
AccessioniPrimary (citable) accession number: P23565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 1, 1996
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.