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P23562 (B3AT_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name=AE 1
Short name=Anion exchanger 1
Solute carrier family 4 member 1
CD_antigen=CD233
Gene names
Name:Slc4a1
Synonyms:Ae1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length927 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine By similarity.

Subunit structure

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity. Basolateral cell membrane; Multi-pass membrane protein By similarity. Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney By similarity.

Tissue specificity

Kidney.

Sequence similarities

Belongs to the anion exchanger (TC 2.A.31) family. [View classification]

Sequence caution

The sequence AAA40800.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAnion exchange
Ion transport
Transport
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
Transmembrane helix
   PTMAcetylation
Glycoprotein
Lipoprotein
Palmitate
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanion transport

Inferred from sequence or structural similarity. Source: UniProtKB

bicarbonate transport

Inferred from sequence or structural similarity. Source: UniProtKB

chloride transmembrane transport

Inferred from sequence or structural similarity. Source: GOC

chloride transport

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentZ disc

Inferred from direct assay PubMed 12898519. Source: RGD

basolateral plasma membrane

Inferred from direct assay PubMed 16144966PubMed 16352747PubMed 17804457. Source: UniProtKB

cortical cytoskeleton

Inferred from sequence or structural similarity. Source: UniProtKB

integral component of plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionactin binding

Inferred from physical interaction PubMed 12898519. Source: RGD

anion:anion antiporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

bicarbonate transmembrane transporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

chloride transmembrane transporter activity

Inferred from sequence or structural similarity. Source: UniProtKB

inorganic anion exchanger activity

Inferred from sequence or structural similarity. Source: UniProtKB

protein C-terminus binding

Inferred from physical interaction PubMed 12898519. Source: RGD

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Erythrocyte (identifier: P23562-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Kidney (identifier: P23562-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 927927Band 3 anion transport protein
PRO_0000079211

Regions

Topological domain1 – 420420Cytoplasmic Potential
Transmembrane421 – 44121Helical; Potential
Transmembrane454 – 47320Helical; Potential
Transmembrane477 – 49620Helical; Potential
Transmembrane508 – 52720Helical; Potential
Transmembrane540 – 55819Helical; Potential
Topological domain559 – 58426Extracellular Potential
Transmembrane585 – 60420Helical; Potential
Topological domain605 – 61915Cytoplasmic Potential
Transmembrane620 – 64021Helical; Potential
Topological domain641 – 67636Extracellular Potential
Transmembrane677 – 69620Helical; Potential
Transmembrane715 – 73521Helical; Potential
Transmembrane779 – 79618Helical; Potential
Transmembrane801 – 82222Helical; Potential
Transmembrane860 – 88122Helical; Potential
Region69 – 303235Globular By similarity
Region190 – 19910Interaction with ANK1 By similarity
Region317 – 37054Dimerization arm By similarity
Region421 – 927507Membrane (anion exchange)

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue311Phosphotyrosine By similarity
Modified residue561Phosphotyrosine By similarity
Modified residue3721Phosphotyrosine By similarity
Modified residue9201Phosphotyrosine By similarity
Lipidation8591S-palmitoyl cysteine By similarity
Glycosylation6581N-linked (GlcNAc...) Probable

Natural variations

Alternative sequence1 – 7979Missing in isoform Kidney.
VSP_000455

Sequences

Sequence LengthMass (Da)Tools
Isoform Erythrocyte [UniParc].

Last modified March 27, 2002. Version 3.
Checksum: 681A228474E5E9DE

FASTA927103,173
        10         20         30         40         50         60 
MGDMQDHEKV LEIPDRDSEE ELEHVIEQIA YRDLDIPVTE MQESEALPTE QTATDYIPTS 

        70         80         90        100        110        120 
TSTSHPSSSQ VYVELQELMM DQRNQELQWV EAAHWIGLEE NLREDGVWGR PHLSYLTFWS 

       130        140        150        160        170        180 
LLELQKVFSK GTFLLDLAET SLAGVANKLL DSFIYEDQIR PQDRDELLRA LLLKRSHAED 

       190        200        210        220        230        240 
LKDLEGVKPA VLTRSGAPSE PLLPHQPSLE TKLYCAQAEG GSEEPSPSGI LKIPPNSETT 

       250        260        270        280        290        300 
LVLVGRASFL VKPVLGFVRL KEAVPLEDLV LPEPVSFLLV LLGPEAPHID YTQLGRAAAT 

       310        320        330        340        350        360 
LMTERVFRVT ASLAQSRGEL LSSLDSFLDC SLVLPPTEAP SEKALLNLVP VQKELLRKRY 

       370        380        390        400        410        420 
LPRPAKPDPN LYEALDGGKE GPGDEDDPLR RTGRIFGGLI RDIRRRYPYY LSDITDALSP 

       430        440        450        460        470        480 
QVLAAVIFIY FAALSPAVTF GGLLGEKTRN LMGVSELLIS TAVQGILFAL LGAQPLLVLG 

       490        500        510        520        530        540 
FSGPLLVFEE AFYSFCESNN LEYIVGRAWI GFWLILLVVL VVAFEGSFLV QYISRYTQEI 

       550        560        570        580        590        600 
FSFLISLIFI YETFSKLIKI FQDYPLQESY APVVMKPKPQ GPVPNTALLS LVLMVGTFLL 

       610        620        630        640        650        660 
AMMLRKFKNS TYFPGKLRRV IGDFGVPISI LIMVLVDTFI KNTYTQKLSV PDGLKVSNSS 

       670        680        690        700        710        720 
ARGWVIHPLG LYNHFPKWMM FASVLPALLV FILIFLESQI TTLIVSKPER KMIKGSGFHL 

       730        740        750        760        770        780 
DLLLVVGMGG VAALFGMPWL SATTVRSVTH ANALTVMGKA SGPGAAAQIQ EVKEQRISGL 

       790        800        810        820        830        840 
LVSVLVGLSI LMEPILSRIP LAVLFGIFLY MGITSLSGIQ LFDRILLLFK PPKYHPDVPF 

       850        860        870        880        890        900 
VKRVKTWRMH LFTGIQIICL AVLWVVKSTP ASLALPFVLI LTVPLRRLLL PLIFRELELQ 

       910        920 
CLDGDDAKVT FDEAEGLDEY DEVPMPV 

« Hide

Isoform Kidney [UniParc].

Checksum: 28FE0DA29BA74027
Show »

FASTA84894,153

References

[1]"Primary structure of the rat kidney band 3 anion exchange protein deduced from a cDNA."
Kudrycki K.E., Shull G.E.
J. Biol. Chem. 264:8185-8192(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-927.
Tissue: Kidney.
[2]"Rat kidney band 3 Cl-/HCO3- exchanger mRNA is transcribed from an alternative promoter."
Kudrycki K.E., Shull G.E.
Am. J. Physiol. 264:F540-F547(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-45.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
L02943 mRNA. Translation: AAA40801.1.
PIRA33810.
A48854.
RefSeqNP_036783.2. NM_012651.2.
XP_006247317.1. XM_006247255.1. [P23562-1]
UniGeneRn.32202.

3D structure databases

ProteinModelPortalP23562.
SMRP23562. Positions 70-368, 406-447, 819-851.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP23562.

Proteomic databases

PaxDbP23562.
PRIDEP23562.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID24779.
KEGGrno:24779.
UCSCRGD:3710. rat. [P23562-1]

Organism-specific databases

CTD6521.
RGD3710. Slc4a1.

Phylogenomic databases

eggNOGNOG268067.
HOGENOMHOG000280683.
HOVERGENHBG004326.
InParanoidP23562.
KOK06573.
PhylomeDBP23562.

Gene expression databases

GenevestigatorP23562.

Family and domain databases

Gene3D3.40.1100.10. 1 hit.
InterProIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERPTHR11453. PTHR11453. 1 hit.
PfamPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMSSF55804. SSF55804. 1 hit.
TIGRFAMsTIGR00834. ae. 1 hit.
PROSITEPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio604378.
PROP23562.

Entry information

Entry nameB3AT_RAT
AccessionPrimary (citable) accession number: P23562
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: March 27, 2002
Last modified: June 11, 2014
This is version 118 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families