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Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine (By similarity).By similarity

GO - Molecular functioni

  1. actin binding Source: RGD
  2. anion:anion antiporter activity Source: UniProtKB
  3. anion transmembrane transporter activity Source: GO_Central
  4. bicarbonate transmembrane transporter activity Source: UniProtKB
  5. chloride transmembrane transporter activity Source: UniProtKB
  6. inorganic anion exchanger activity Source: UniProtKB
  7. protein C-terminus binding Source: RGD

GO - Biological processi

  1. anion transport Source: UniProtKB
  2. bicarbonate transport Source: UniProtKB
  3. chloride transmembrane transport Source: GO_Central
  4. chloride transport Source: UniProtKB
  5. regulation of intracellular pH Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Transport

Enzyme and pathway databases

ReactomeiREACT_199188. Erythrocytes take up carbon dioxide and release oxygen.
REACT_199216. Erythrocytes take up oxygen and release carbon dioxide.
REACT_260612. Bicarbonate transporters.

Names & Taxonomyi

Protein namesi
Recommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name:
AE 1
Short name:
Anion exchanger 1
Solute carrier family 4 member 1
CD_antigen: CD233
Gene namesi
Name:Slc4a1
Synonyms:Ae1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi3710. Slc4a1.

Subcellular locationi

Cell membrane By similarity; Multi-pass membrane protein By similarity. Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 420420CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei421 – 44121HelicalSequence AnalysisAdd
BLAST
Transmembranei454 – 47320HelicalSequence AnalysisAdd
BLAST
Transmembranei477 – 49620HelicalSequence AnalysisAdd
BLAST
Transmembranei508 – 52720HelicalSequence AnalysisAdd
BLAST
Transmembranei540 – 55819HelicalSequence AnalysisAdd
BLAST
Topological domaini559 – 58426ExtracellularSequence AnalysisAdd
BLAST
Transmembranei585 – 60420HelicalSequence AnalysisAdd
BLAST
Topological domaini605 – 61915CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei620 – 64021HelicalSequence AnalysisAdd
BLAST
Topological domaini641 – 67636ExtracellularSequence AnalysisAdd
BLAST
Transmembranei677 – 69620HelicalSequence AnalysisAdd
BLAST
Transmembranei715 – 73521HelicalSequence AnalysisAdd
BLAST
Transmembranei779 – 79618HelicalSequence AnalysisAdd
BLAST
Transmembranei801 – 82222HelicalSequence AnalysisAdd
BLAST
Transmembranei860 – 88122HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. blood microparticle Source: Ensembl
  3. cortical cytoskeleton Source: UniProtKB
  4. extracellular vesicular exosome Source: Ensembl
  5. integral component of plasma membrane Source: UniProtKB
  6. Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Band 3 anion transport proteinPRO_0000079211Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei31 – 311PhosphotyrosineBy similarity
Modified residuei56 – 561PhosphotyrosineBy similarity
Modified residuei372 – 3721PhosphotyrosineBy similarity
Glycosylationi658 – 6581N-linked (GlcNAc...)Curated
Lipidationi859 – 8591S-palmitoyl cysteineBy similarity
Modified residuei920 – 9201PhosphotyrosineBy similarity

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP23562.
PRIDEiP23562.

PTM databases

PhosphoSiteiP23562.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

ExpressionAtlasiP23562. baseline and differential.
GenevestigatoriP23562.

Interactioni

Subunit structurei

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP23562.
SMRiP23562. Positions 70-368, 406-447, 819-851.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni69 – 303235GlobularBy similarityAdd
BLAST
Regioni190 – 19910Interaction with ANK1By similarity
Regioni317 – 37054Dimerization armBy similarityAdd
BLAST
Regioni421 – 927507Membrane (anion exchange)Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG268067.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP23562.
KOiK06573.
PhylomeDBiP23562.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Erythrocyte (identifier: P23562-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMQDHEKV LEIPDRDSEE ELEHVIEQIA YRDLDIPVTE MQESEALPTE
60 70 80 90 100
QTATDYIPTS TSTSHPSSSQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLDLAET SLAGVANKLL
160 170 180 190 200
DSFIYEDQIR PQDRDELLRA LLLKRSHAED LKDLEGVKPA VLTRSGAPSE
210 220 230 240 250
PLLPHQPSLE TKLYCAQAEG GSEEPSPSGI LKIPPNSETT LVLVGRASFL
260 270 280 290 300
VKPVLGFVRL KEAVPLEDLV LPEPVSFLLV LLGPEAPHID YTQLGRAAAT
310 320 330 340 350
LMTERVFRVT ASLAQSRGEL LSSLDSFLDC SLVLPPTEAP SEKALLNLVP
360 370 380 390 400
VQKELLRKRY LPRPAKPDPN LYEALDGGKE GPGDEDDPLR RTGRIFGGLI
410 420 430 440 450
RDIRRRYPYY LSDITDALSP QVLAAVIFIY FAALSPAVTF GGLLGEKTRN
460 470 480 490 500
LMGVSELLIS TAVQGILFAL LGAQPLLVLG FSGPLLVFEE AFYSFCESNN
510 520 530 540 550
LEYIVGRAWI GFWLILLVVL VVAFEGSFLV QYISRYTQEI FSFLISLIFI
560 570 580 590 600
YETFSKLIKI FQDYPLQESY APVVMKPKPQ GPVPNTALLS LVLMVGTFLL
610 620 630 640 650
AMMLRKFKNS TYFPGKLRRV IGDFGVPISI LIMVLVDTFI KNTYTQKLSV
660 670 680 690 700
PDGLKVSNSS ARGWVIHPLG LYNHFPKWMM FASVLPALLV FILIFLESQI
710 720 730 740 750
TTLIVSKPER KMIKGSGFHL DLLLVVGMGG VAALFGMPWL SATTVRSVTH
760 770 780 790 800
ANALTVMGKA SGPGAAAQIQ EVKEQRISGL LVSVLVGLSI LMEPILSRIP
810 820 830 840 850
LAVLFGIFLY MGITSLSGIQ LFDRILLLFK PPKYHPDVPF VKRVKTWRMH
860 870 880 890 900
LFTGIQIICL AVLWVVKSTP ASLALPFVLI LTVPLRRLLL PLIFRELELQ
910 920
CLDGDDAKVT FDEAEGLDEY DEVPMPV
Length:927
Mass (Da):103,173
Last modified:March 27, 2002 - v3
Checksum:i681A228474E5E9DE
GO
Isoform Kidney (identifier: P23562-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Show »
Length:848
Mass (Da):94,153
Checksum:i28FE0DA29BA74027
GO

Sequence cautioni

The sequence AAA40800.1 differs from that shown. Reason: Erroneous initiation. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7979Missing in isoform Kidney. CuratedVSP_000455Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
L02943 mRNA. Translation: AAA40801.1.
PIRiA33810.
A48854.
RefSeqiNP_036783.2. NM_012651.2.
XP_008766168.1. XM_008767946.1. [P23562-1]
UniGeneiRn.32202.

Genome annotation databases

GeneIDi24779.
KEGGirno:24779.
UCSCiRGD:3710. rat. [P23562-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
L02943 mRNA. Translation: AAA40801.1.
PIRiA33810.
A48854.
RefSeqiNP_036783.2. NM_012651.2.
XP_008766168.1. XM_008767946.1. [P23562-1]
UniGeneiRn.32202.

3D structure databases

ProteinModelPortaliP23562.
SMRiP23562. Positions 70-368, 406-447, 819-851.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiP23562.

Proteomic databases

PaxDbiP23562.
PRIDEiP23562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24779.
KEGGirno:24779.
UCSCiRGD:3710. rat. [P23562-1]

Organism-specific databases

CTDi6521.
RGDi3710. Slc4a1.

Phylogenomic databases

eggNOGiNOG268067.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP23562.
KOiK06573.
PhylomeDBiP23562.

Enzyme and pathway databases

ReactomeiREACT_199188. Erythrocytes take up carbon dioxide and release oxygen.
REACT_199216. Erythrocytes take up oxygen and release carbon dioxide.
REACT_260612. Bicarbonate transporters.

Miscellaneous databases

NextBioi604378.
PROiP23562.

Gene expression databases

ExpressionAtlasiP23562. baseline and differential.
GenevestigatoriP23562.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of the rat kidney band 3 anion exchange protein deduced from a cDNA."
    Kudrycki K.E., Shull G.E.
    J. Biol. Chem. 264:8185-8192(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-927.
    Tissue: Kidney.
  2. "Rat kidney band 3 Cl-/HCO3- exchanger mRNA is transcribed from an alternative promoter."
    Kudrycki K.E., Shull G.E.
    Am. J. Physiol. 264:F540-F547(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-45.

Entry informationi

Entry nameiB3AT_RAT
AccessioniPrimary (citable) accession number: P23562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: March 27, 2002
Last modified: March 4, 2015
This is version 124 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.