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Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.By similarity

GO - Molecular functioni

GO - Biological processi

  • anion transport Source: UniProtKB
  • bicarbonate transport Source: UniProtKB
  • chloride transmembrane transport Source: GO_Central
  • chloride transport Source: UniProtKB
  • circadian rhythm Source: RGD
  • positive regulation of T cell proliferation Source: RGD
  • protein oligomerization Source: RGD
  • regulation of intracellular pH Source: GO_Central
  • response to activity Source: RGD
  • response to arsenic-containing substance Source: RGD
  • response to carbon dioxide Source: RGD
  • response to hydrogen peroxide Source: RGD
  • response to nutrient levels Source: RGD
  • response to water deprivation Source: RGD
Complete GO annotation...

Keywords - Biological processi

Anion exchange, Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Band 3 anion transport protein
Alternative name(s):
Anion exchange protein 1
Short name:
AE 1
Short name:
Anion exchanger 1
Solute carrier family 4 member 1
CD_antigen: CD233
Gene namesi
Name:Slc4a1
Synonyms:Ae1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3710. Slc4a1.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity

  • Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 420CytoplasmicBy similarityAdd BLAST420
Transmembranei421 – 444Helical; Name=1By similarityAdd BLAST24
Topological domaini445 – 452ExtracellularBy similarity8
Transmembranei453 – 473Helical; Name=2By similarityAdd BLAST21
Topological domaini474 – 476CytoplasmicBy similarity3
Transmembranei477 – 493Discontinuously helical; Name=3By similarityAdd BLAST17
Topological domaini494 – 502ExtracellularBy similarity9
Transmembranei503 – 523Helical; Name=4By similarityAdd BLAST21
Topological domaini524 – 535CytoplasmicBy similarityAdd BLAST12
Transmembranei536 – 558Helical; Name=5By similarityAdd BLAST23
Topological domaini559 – 586ExtracellularBy similarityAdd BLAST28
Transmembranei587 – 607Helical; Name=6By similarityAdd BLAST21
Topological domaini608 – 618CytoplasmicBy similarityAdd BLAST11
Transmembranei619 – 639Helical; Name=7By similarityAdd BLAST21
Topological domaini640 – 679ExtracellularBy similarityAdd BLAST40
Transmembranei680 – 700Helical; Name=8By similarityAdd BLAST21
Topological domaini701 – 716CytoplasmicBy similarityAdd BLAST16
Transmembranei717 – 735Helical; Name=9By similarityAdd BLAST19
Transmembranei736 – 753Discontinuously helical; Name=10By similarityAdd BLAST18
Topological domaini754 – 776CytoplasmicBy similarityAdd BLAST23
Transmembranei777 – 797Helical; Name=11By similarityAdd BLAST21
Transmembranei798 – 816Helical; Name=12By similarityAdd BLAST19
Topological domaini817 – 854CytoplasmicBy similarityAdd BLAST38
Intramembranei855 – 885Discontinuously helicalBy similarityAdd BLAST31
Topological domaini886 – 927CytoplasmicBy similarityAdd BLAST42

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB
  • cell surface Source: RGD
  • cortical cytoskeleton Source: UniProtKB
  • integral component of plasma membrane Source: UniProtKB
  • intercalated disc Source: RGD
  • plasma membrane Source: RGD
  • Z disc Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000792111 – 927Band 3 anion transport proteinAdd BLAST927

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei18PhosphoserineCombined sources1
Modified residuei31PhosphotyrosineBy similarity1
Modified residuei56PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei372PhosphotyrosineBy similarity1
Glycosylationi658N-linked (GlcNAc...)Curated1
Lipidationi859S-palmitoyl cysteineBy similarity1
Modified residuei920PhosphotyrosineBy similarity1

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP23562.
PeptideAtlasiP23562.
PRIDEiP23562.

PTM databases

iPTMnetiP23562.
PhosphoSitePlusiP23562.

Expressioni

Tissue specificityi

Kidney.

Gene expression databases

BgeeiENSRNOG00000020951.

Interactioni

Subunit structurei

A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM. Isoform 2 interacts with TMEM139.By similarity

GO - Molecular functioni

  • actin binding Source: RGD
  • enzyme binding Source: RGD
  • protein C-terminus binding Source: RGD

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028445.

Structurei

3D structure databases

ProteinModelPortaliP23562.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni69 – 303GlobularBy similarityAdd BLAST235
Regioni190 – 199Interaction with ANK1By similarity10
Regioni317 – 370Dimerization armBy similarityAdd BLAST54

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP23562.
KOiK06573.
PhylomeDBiP23562.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23562-1) [UniParc]FASTAAdd to basket
Also known as: Erythrocyte

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGDMQDHEKV LEIPDRDSEE ELEHVIEQIA YRDLDIPVTE MQESEALPTE
60 70 80 90 100
QTATDYIPTS TSTSHPSSSQ VYVELQELMM DQRNQELQWV EAAHWIGLEE
110 120 130 140 150
NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLDLAET SLAGVANKLL
160 170 180 190 200
DSFIYEDQIR PQDRDELLRA LLLKRSHAED LKDLEGVKPA VLTRSGAPSE
210 220 230 240 250
PLLPHQPSLE TKLYCAQAEG GSEEPSPSGI LKIPPNSETT LVLVGRASFL
260 270 280 290 300
VKPVLGFVRL KEAVPLEDLV LPEPVSFLLV LLGPEAPHID YTQLGRAAAT
310 320 330 340 350
LMTERVFRVT ASLAQSRGEL LSSLDSFLDC SLVLPPTEAP SEKALLNLVP
360 370 380 390 400
VQKELLRKRY LPRPAKPDPN LYEALDGGKE GPGDEDDPLR RTGRIFGGLI
410 420 430 440 450
RDIRRRYPYY LSDITDALSP QVLAAVIFIY FAALSPAVTF GGLLGEKTRN
460 470 480 490 500
LMGVSELLIS TAVQGILFAL LGAQPLLVLG FSGPLLVFEE AFYSFCESNN
510 520 530 540 550
LEYIVGRAWI GFWLILLVVL VVAFEGSFLV QYISRYTQEI FSFLISLIFI
560 570 580 590 600
YETFSKLIKI FQDYPLQESY APVVMKPKPQ GPVPNTALLS LVLMVGTFLL
610 620 630 640 650
AMMLRKFKNS TYFPGKLRRV IGDFGVPISI LIMVLVDTFI KNTYTQKLSV
660 670 680 690 700
PDGLKVSNSS ARGWVIHPLG LYNHFPKWMM FASVLPALLV FILIFLESQI
710 720 730 740 750
TTLIVSKPER KMIKGSGFHL DLLLVVGMGG VAALFGMPWL SATTVRSVTH
760 770 780 790 800
ANALTVMGKA SGPGAAAQIQ EVKEQRISGL LVSVLVGLSI LMEPILSRIP
810 820 830 840 850
LAVLFGIFLY MGITSLSGIQ LFDRILLLFK PPKYHPDVPF VKRVKTWRMH
860 870 880 890 900
LFTGIQIICL AVLWVVKSTP ASLALPFVLI LTVPLRRLLL PLIFRELELQ
910 920
CLDGDDAKVT FDEAEGLDEY DEVPMPV
Length:927
Mass (Da):103,173
Last modified:March 27, 2002 - v3
Checksum:i681A228474E5E9DE
GO
Isoform 2 (identifier: P23562-2) [UniParc]FASTAAdd to basket
Also known as: Kidney

The sequence of this isoform differs from the canonical sequence as follows:
     1-79: Missing.

Show »
Length:848
Mass (Da):94,153
Checksum:i28FE0DA29BA74027
GO

Sequence cautioni

The sequence AAA40800 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0004551 – 79Missing in isoform 2. CuratedAdd BLAST79

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
L02943 mRNA. Translation: AAA40801.1.
PIRiA33810.
A48854.
RefSeqiNP_036783.2. NM_012651.2.
XP_008766168.1. XM_008767946.2. [P23562-1]
UniGeneiRn.32202.

Genome annotation databases

GeneIDi24779.
KEGGirno:24779.
UCSCiRGD:3710. rat. [P23562-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
L02943 mRNA. Translation: AAA40801.1.
PIRiA33810.
A48854.
RefSeqiNP_036783.2. NM_012651.2.
XP_008766168.1. XM_008767946.2. [P23562-1]
UniGeneiRn.32202.

3D structure databases

ProteinModelPortaliP23562.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000028445.

PTM databases

iPTMnetiP23562.
PhosphoSitePlusiP23562.

Proteomic databases

PaxDbiP23562.
PeptideAtlasiP23562.
PRIDEiP23562.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24779.
KEGGirno:24779.
UCSCiRGD:3710. rat. [P23562-1]

Organism-specific databases

CTDi6521.
RGDi3710. Slc4a1.

Phylogenomic databases

eggNOGiKOG1172. Eukaryota.
ENOG410XPHD. LUCA.
HOGENOMiHOG000280683.
HOVERGENiHBG004326.
InParanoidiP23562.
KOiK06573.
PhylomeDBiP23562.

Miscellaneous databases

PROiP23562.

Gene expression databases

BgeeiENSRNOG00000020951.

Family and domain databases

Gene3Di3.40.1100.10. 1 hit.
InterProiIPR001717. Anion_exchange.
IPR002977. Anion_exchange_1.
IPR018241. Anion_exchange_CS.
IPR013769. Band3_cytoplasmic_dom.
IPR011531. HCO3_transpt_C.
IPR003020. HCO3_transpt_euk.
IPR016152. PTrfase/Anion_transptr.
[Graphical view]
PANTHERiPTHR11453. PTHR11453. 1 hit.
PfamiPF07565. Band_3_cyto. 1 hit.
PF00955. HCO3_cotransp. 2 hits.
[Graphical view]
PRINTSiPR00165. ANIONEXCHNGR.
PR01187. ANIONEXHNGR1.
PR01231. HCO3TRNSPORT.
SUPFAMiSSF55804. SSF55804. 1 hit.
TIGRFAMsiTIGR00834. ae. 1 hit.
PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
PS00220. ANION_EXCHANGER_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiB3AT_RAT
AccessioniPrimary (citable) accession number: P23562
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: March 27, 2002
Last modified: November 2, 2016
This is version 142 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.