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P23562

- B3AT_RAT

UniProt

P23562 - B3AT_RAT

Protein

Band 3 anion transport protein

Gene

Slc4a1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 3 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine By similarity.By similarity

    GO - Molecular functioni

    1. actin binding Source: RGD
    2. anion:anion antiporter activity Source: UniProtKB
    3. bicarbonate transmembrane transporter activity Source: UniProtKB
    4. chloride transmembrane transporter activity Source: UniProtKB
    5. inorganic anion exchanger activity Source: UniProtKB
    6. protein C-terminus binding Source: RGD

    GO - Biological processi

    1. anion transport Source: UniProtKB
    2. bicarbonate transport Source: UniProtKB
    3. chloride transmembrane transport Source: GOC
    4. chloride transport Source: UniProtKB

    Keywords - Biological processi

    Anion exchange, Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_199188. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_199216. Erythrocytes take up oxygen and release carbon dioxide.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Band 3 anion transport protein
    Alternative name(s):
    Anion exchange protein 1
    Short name:
    AE 1
    Short name:
    Anion exchanger 1
    Solute carrier family 4 member 1
    CD_antigen: CD233
    Gene namesi
    Name:Slc4a1
    Synonyms:Ae1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi3710. Slc4a1.

    Subcellular locationi

    Cell membrane By similarity; Multi-pass membrane protein By similarity. Basolateral cell membrane By similarity; Multi-pass membrane protein By similarity
    Note: Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. cortical cytoskeleton Source: UniProtKB
    3. integral component of plasma membrane Source: UniProtKB
    4. Z disc Source: RGD

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 927927Band 3 anion transport proteinPRO_0000079211Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei31 – 311PhosphotyrosineBy similarity
    Modified residuei56 – 561PhosphotyrosineBy similarity
    Modified residuei372 – 3721PhosphotyrosineBy similarity
    Glycosylationi658 – 6581N-linked (GlcNAc...)Curated
    Lipidationi859 – 8591S-palmitoyl cysteineBy similarity
    Modified residuei920 – 9201PhosphotyrosineBy similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiP23562.
    PRIDEiP23562.

    PTM databases

    PhosphoSiteiP23562.

    Expressioni

    Tissue specificityi

    Kidney.

    Gene expression databases

    GenevestigatoriP23562.

    Interactioni

    Subunit structurei

    A dimer in solution, but in its membrane environment, it exists primarily as a mixture of dimers and tetramers and spans the membrane asymmetrically. Interacts (via cytoplasmic N-terminal domain) with ANK1 (via N-terminal ANK repeats); tetramer formation is critical for ankyrin association. Interacts with STOM By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP23562.
    SMRiP23562. Positions 70-368, 406-447, 819-851.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 420420CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini559 – 58426ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini605 – 61915CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini641 – 67636ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei421 – 44121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei454 – 47320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei477 – 49620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei508 – 52720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei540 – 55819HelicalSequence AnalysisAdd
    BLAST
    Transmembranei585 – 60420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei620 – 64021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei677 – 69620HelicalSequence AnalysisAdd
    BLAST
    Transmembranei715 – 73521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei779 – 79618HelicalSequence AnalysisAdd
    BLAST
    Transmembranei801 – 82222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei860 – 88122HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni69 – 303235GlobularBy similarityAdd
    BLAST
    Regioni190 – 19910Interaction with ANK1By similarity
    Regioni317 – 37054Dimerization armBy similarityAdd
    BLAST
    Regioni421 – 927507Membrane (anion exchange)Add
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG268067.
    HOGENOMiHOG000280683.
    HOVERGENiHBG004326.
    InParanoidiP23562.
    KOiK06573.
    PhylomeDBiP23562.

    Family and domain databases

    Gene3Di3.40.1100.10. 1 hit.
    InterProiIPR001717. Anion_exchange.
    IPR002977. Anion_exchange_1.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view]
    PANTHERiPTHR11453. PTHR11453. 1 hit.
    PfamiPF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view]
    PRINTSiPR00165. ANIONEXCHNGR.
    PR01187. ANIONEXHNGR1.
    PR01231. HCO3TRNSPORT.
    SUPFAMiSSF55804. SSF55804. 1 hit.
    TIGRFAMsiTIGR00834. ae. 1 hit.
    PROSITEiPS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Erythrocyte (identifier: P23562-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGDMQDHEKV LEIPDRDSEE ELEHVIEQIA YRDLDIPVTE MQESEALPTE    50
    QTATDYIPTS TSTSHPSSSQ VYVELQELMM DQRNQELQWV EAAHWIGLEE 100
    NLREDGVWGR PHLSYLTFWS LLELQKVFSK GTFLLDLAET SLAGVANKLL 150
    DSFIYEDQIR PQDRDELLRA LLLKRSHAED LKDLEGVKPA VLTRSGAPSE 200
    PLLPHQPSLE TKLYCAQAEG GSEEPSPSGI LKIPPNSETT LVLVGRASFL 250
    VKPVLGFVRL KEAVPLEDLV LPEPVSFLLV LLGPEAPHID YTQLGRAAAT 300
    LMTERVFRVT ASLAQSRGEL LSSLDSFLDC SLVLPPTEAP SEKALLNLVP 350
    VQKELLRKRY LPRPAKPDPN LYEALDGGKE GPGDEDDPLR RTGRIFGGLI 400
    RDIRRRYPYY LSDITDALSP QVLAAVIFIY FAALSPAVTF GGLLGEKTRN 450
    LMGVSELLIS TAVQGILFAL LGAQPLLVLG FSGPLLVFEE AFYSFCESNN 500
    LEYIVGRAWI GFWLILLVVL VVAFEGSFLV QYISRYTQEI FSFLISLIFI 550
    YETFSKLIKI FQDYPLQESY APVVMKPKPQ GPVPNTALLS LVLMVGTFLL 600
    AMMLRKFKNS TYFPGKLRRV IGDFGVPISI LIMVLVDTFI KNTYTQKLSV 650
    PDGLKVSNSS ARGWVIHPLG LYNHFPKWMM FASVLPALLV FILIFLESQI 700
    TTLIVSKPER KMIKGSGFHL DLLLVVGMGG VAALFGMPWL SATTVRSVTH 750
    ANALTVMGKA SGPGAAAQIQ EVKEQRISGL LVSVLVGLSI LMEPILSRIP 800
    LAVLFGIFLY MGITSLSGIQ LFDRILLLFK PPKYHPDVPF VKRVKTWRMH 850
    LFTGIQIICL AVLWVVKSTP ASLALPFVLI LTVPLRRLLL PLIFRELELQ 900
    CLDGDDAKVT FDEAEGLDEY DEVPMPV 927
    Length:927
    Mass (Da):103,173
    Last modified:March 27, 2002 - v3
    Checksum:i681A228474E5E9DE
    GO
    Isoform Kidney (identifier: P23562-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-79: Missing.

    Show »
    Length:848
    Mass (Da):94,153
    Checksum:i28FE0DA29BA74027
    GO

    Sequence cautioni

    The sequence AAA40800.1 differs from that shown. Reason: Erroneous initiation.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 7979Missing in isoform Kidney. CuratedVSP_000455Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04793 Genomic DNA. Translation: AAA40800.1. Different initiation.
    L02943 mRNA. Translation: AAA40801.1.
    PIRiA33810.
    A48854.
    RefSeqiNP_036783.2. NM_012651.2.
    XP_006247317.1. XM_006247255.1. [P23562-1]
    UniGeneiRn.32202.

    Genome annotation databases

    GeneIDi24779.
    KEGGirno:24779.
    UCSCiRGD:3710. rat. [P23562-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J04793 Genomic DNA. Translation: AAA40800.1 . Different initiation.
    L02943 mRNA. Translation: AAA40801.1 .
    PIRi A33810.
    A48854.
    RefSeqi NP_036783.2. NM_012651.2.
    XP_006247317.1. XM_006247255.1. [P23562-1 ]
    UniGenei Rn.32202.

    3D structure databases

    ProteinModelPortali P23562.
    SMRi P23562. Positions 70-368, 406-447, 819-851.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P23562.

    Proteomic databases

    PaxDbi P23562.
    PRIDEi P23562.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 24779.
    KEGGi rno:24779.
    UCSCi RGD:3710. rat. [P23562-1 ]

    Organism-specific databases

    CTDi 6521.
    RGDi 3710. Slc4a1.

    Phylogenomic databases

    eggNOGi NOG268067.
    HOGENOMi HOG000280683.
    HOVERGENi HBG004326.
    InParanoidi P23562.
    KOi K06573.
    PhylomeDBi P23562.

    Enzyme and pathway databases

    Reactomei REACT_199188. Erythrocytes take up carbon dioxide and release oxygen.
    REACT_199216. Erythrocytes take up oxygen and release carbon dioxide.

    Miscellaneous databases

    NextBioi 604378.
    PROi P23562.

    Gene expression databases

    Genevestigatori P23562.

    Family and domain databases

    Gene3Di 3.40.1100.10. 1 hit.
    InterProi IPR001717. Anion_exchange.
    IPR002977. Anion_exchange_1.
    IPR018241. Anion_exchange_CS.
    IPR013769. Band3_cytoplasmic_dom.
    IPR011531. HCO3_transpt_C.
    IPR003020. HCO3_transpt_euk.
    IPR016152. PTrfase/Anion_transptr.
    [Graphical view ]
    PANTHERi PTHR11453. PTHR11453. 1 hit.
    Pfami PF07565. Band_3_cyto. 1 hit.
    PF00955. HCO3_cotransp. 2 hits.
    [Graphical view ]
    PRINTSi PR00165. ANIONEXCHNGR.
    PR01187. ANIONEXHNGR1.
    PR01231. HCO3TRNSPORT.
    SUPFAMi SSF55804. SSF55804. 1 hit.
    TIGRFAMsi TIGR00834. ae. 1 hit.
    PROSITEi PS00219. ANION_EXCHANGER_1. 1 hit.
    PS00220. ANION_EXCHANGER_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the rat kidney band 3 anion exchange protein deduced from a cDNA."
      Kudrycki K.E., Shull G.E.
      J. Biol. Chem. 264:8185-8192(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 46-927.
      Tissue: Kidney.
    2. "Rat kidney band 3 Cl-/HCO3- exchanger mRNA is transcribed from an alternative promoter."
      Kudrycki K.E., Shull G.E.
      Am. J. Physiol. 264:F540-F547(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-45.

    Entry informationi

    Entry nameiB3AT_RAT
    AccessioniPrimary (citable) accession number: P23562
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 120 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3