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P23561

- STE11_YEAST

UniProt

P23561 - STE11_YEAST

Protein

Serine/threonine-protein kinase STE11

Gene

STE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 153 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it phosphorylates the STE7 protein kinase which itself, phosphorylates the FUS3 and or KSS1 kinases.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei444 – 4441ATPPROSITE-ProRule annotation
    Active sitei579 – 5791Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi421 – 4299ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. identical protein binding Source: IntAct
    3. MAP kinase kinase kinase activity Source: SGD
    4. protein binding Source: IntAct
    5. SAM domain binding Source: SGD

    GO - Biological processi

    1. activation of MAPKK activity Source: SGD
    2. cellular response to heat Source: SGD
    3. invasive growth in response to glucose limitation Source: SGD
    4. MAPK cascade involved in cell wall organization or biogenesis Source: SGD
    5. MAPK cascade involved in osmosensory signaling pathway Source: SGD
    6. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
    7. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
    8. protein phosphorylation Source: SGD
    9. pseudohyphal growth Source: SGD
    10. regulation of transposition, RNA-mediated Source: SGD
    11. signal transduction involved in filamentous growth Source: SGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Pheromone response

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-32433-MONOMER.
    BRENDAi2.7.11.25. 984.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase STE11 (EC:2.7.11.25)
    Gene namesi
    Name:STE11
    Ordered Locus Names:YLR362W
    ORF Names:L8039.10
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XII

    Organism-specific databases

    SGDiS000004354. STE11.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi59 – 591I → R: Disrupts interaction with STE50 and abolishes signal transduction. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 717717Serine/threonine-protein kinase STE11PRO_0000086684Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei323 – 3231Phosphoserine2 Publications
    Modified residuei465 – 4651Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP23561.
    PaxDbiP23561.
    PeptideAtlasiP23561.

    Expressioni

    Gene expression databases

    GenevestigatoriP23561.

    Interactioni

    Subunit structurei

    Homodimer Probable. Interacts (via SAM domain) with STE50 (via SAM domain). Interacts with PBS2 and SHO1.3 PublicationsCurated

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself3EBI-18259,EBI-18259
    FUS3P168926EBI-18259,EBI-7193
    KSS1P146814EBI-18259,EBI-9945
    SHO1P400733EBI-18259,EBI-18140
    STE5P329177EBI-18259,EBI-18373
    STE50P253446EBI-18259,EBI-18305

    Protein-protein interaction databases

    BioGridi31623. 107 interactions.
    DIPiDIP-861N.
    IntActiP23561. 19 interactions.
    MINTiMINT-408610.
    STRINGi4932.YLR362W.

    Structurei

    Secondary structure

    1
    717
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi24 – 329
    Helixi36 – 449
    Helixi49 – 546
    Helixi57 – 637
    Helixi68 – 8013

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1OW5NMR-A15-92[»]
    1X9XNMR-A/B16-83[»]
    ProteinModelPortaliP23561.
    SMRiP23561. Positions 22-83, 394-712.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23561.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 8465SAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini415 – 712298Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117243.
    HOGENOMiHOG000172453.
    KOiK11228.
    OMAiVELVTIC.
    OrthoDBiEOG71K6B8.

    Family and domain databases

    Gene3Di1.10.150.50. 1 hit.
    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR029458. Ras-bd_By2.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    PF14847. Ras_bdg_2. 1 hit.
    PF07647. SAM_2. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view]
    SUPFAMiSSF47769. SSF47769. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23561-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQTQTAEGT DLLIGDEKTN DLPFVQLFLE EIGCTQYLDS FIQCNLVTEE    50
    EIKYLDKDIL IALGVNKIGD RLKILRKSKS FQRDKRIEQV NRLKNLMEKV 100
    SSLSTATLSM NSELIPEKHC VIFILNDGSA KKVNVNGCFN ADSIKKRLIR 150
    RLPHELLATN SNGEVTKMVQ DYDVFVLDYT KNVLHLLYDV ELVTICHAND 200
    RVEKNRLIFV SKDQTPSDKA ISTSKKLYLR TLSALSQVGP SSSNLLAQNK 250
    GISHNNAEGK LRIDNTEKDR IRQIFNQRPP SEFISTNLAG YFPHTDMKRL 300
    QKTMRESFRH SARLSIAQRR PLSAESNNIG DILLKHSNAV DMALLQGLDQ 350
    TRLSSKLDTT KIPKLAHKRP EDNDAISNQL ELLSVESGEE EDHDFFGEDS 400
    DIVSLPTKIA TPKNWLKGAC IGSGSFGSVY LGMNAHTGEL MAVKQVEIKN 450
    NNIGVPTDNN KQANSDENNE QEEQQEKIED VGAVSHPKTN QNIHRKMVDA 500
    LQHEMNLLKE LHHENIVTYY GASQEGGNLN IFLEYVPGGS VSSMLNNYGP 550
    FEESLITNFT RQILIGVAYL HKKNIIHRDI KGANILIDIK GCVKITDFGI 600
    SKKLSPLNKK QNKRASLQGS VFWMSPEVVK QTATTAKADI WSTGCVVIEM 650
    FTGKHPFPDF SQMQAIFKIG TNTTPEIPSW ATSEGKNFLR KAFELDYQYR 700
    PSALELLQHP WLDAHII 717
    Length:717
    Mass (Da):80,721
    Last modified:June 16, 2009 - v3
    Checksum:iE9E0DF27114EEEEF
    GO

    Sequence cautioni

    The sequence AAB67571.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53431 Genomic DNA. Translation: CAA37522.1.
    U19103 Genomic DNA. Translation: AAB67571.1. Different initiation.
    BK006945 Genomic DNA. Translation: DAA09666.1.
    PIRiS51380.
    RefSeqiNP_013466.1. NM_001182251.1.

    Genome annotation databases

    EnsemblFungiiYLR362W; YLR362W; YLR362W.
    GeneIDi851076.
    KEGGisce:YLR362W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53431 Genomic DNA. Translation: CAA37522.1 .
    U19103 Genomic DNA. Translation: AAB67571.1 . Different initiation.
    BK006945 Genomic DNA. Translation: DAA09666.1 .
    PIRi S51380.
    RefSeqi NP_013466.1. NM_001182251.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1OW5 NMR - A 15-92 [» ]
    1X9X NMR - A/B 16-83 [» ]
    ProteinModelPortali P23561.
    SMRi P23561. Positions 22-83, 394-712.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31623. 107 interactions.
    DIPi DIP-861N.
    IntActi P23561. 19 interactions.
    MINTi MINT-408610.
    STRINGi 4932.YLR362W.

    Proteomic databases

    MaxQBi P23561.
    PaxDbi P23561.
    PeptideAtlasi P23561.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YLR362W ; YLR362W ; YLR362W .
    GeneIDi 851076.
    KEGGi sce:YLR362W.

    Organism-specific databases

    SGDi S000004354. STE11.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117243.
    HOGENOMi HOG000172453.
    KOi K11228.
    OMAi VELVTIC.
    OrthoDBi EOG71K6B8.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-32433-MONOMER.
    BRENDAi 2.7.11.25. 984.

    Miscellaneous databases

    EvolutionaryTracei P23561.
    NextBioi 967729.

    Gene expression databases

    Genevestigatori P23561.

    Family and domain databases

    Gene3Di 1.10.150.50. 1 hit.
    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR029458. Ras-bd_By2.
    IPR001660. SAM.
    IPR013761. SAM/pointed.
    IPR011510. SAM_2.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    PF14847. Ras_bdg_2. 1 hit.
    PF07647. SAM_2. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    SM00454. SAM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47769. SSF47769. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50105. SAM_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast."
      Rhodes N., Connell L., Errede B.
      Genes Dev. 4:1862-1874(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE.
    2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
      Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
      , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
      Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase."
      Cairns B.R., Ramer S.W., Kornberg K.D.
      Genes Dev. 6:1305-1318(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: POSSIBLE FUNCTION.
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway."
      Zarrinpar A., Bhattacharyya R.P., Nittler M.P., Lim W.A.
      Mol. Cell 14:825-832(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PBS2 AND SHO1.
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The solution structure of the S.cerevisiae Ste11 MAPKKK SAM domain and its partnership with Ste50."
      Kwan J.J., Warner N., Pawson T., Donaldson L.W.
      J. Mol. Biol. 342:681-693(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 15-92, INTERACTION WITH STE50, MUTAGENESIS OF ILE-59.
    11. "Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex."
      Bhattacharjya S., Xu P., Gingras R., Shaykhutdinov R., Wu C., Whiteway M., Ni F.
      J. Mol. Biol. 344:1071-1087(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR OF 16-83, SUBUNIT, INTERACTION WITH STE50.

    Entry informationi

    Entry nameiSTE11_YEAST
    AccessioniPrimary (citable) accession number: P23561
    Secondary accession number(s): D6VZ00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 153 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 736 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    External Data

    Dasty 3