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P23561

- STE11_YEAST

UniProt

P23561 - STE11_YEAST

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Protein

Serine/threonine-protein kinase STE11

Gene

STE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it phosphorylates the STE7 protein kinase which itself, phosphorylates the FUS3 and or KSS1 kinases.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei444 – 4441ATPPROSITE-ProRule annotation
Active sitei579 – 5791Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi421 – 4299ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. identical protein binding Source: IntAct
  3. MAP kinase kinase kinase activity Source: SGD
  4. SAM domain binding Source: SGD

GO - Biological processi

  1. activation of MAPKK activity Source: SGD
  2. cellular response to heat Source: SGD
  3. invasive growth in response to glucose limitation Source: SGD
  4. MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  5. MAPK cascade involved in osmosensory signaling pathway Source: SGD
  6. osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  7. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  8. protein phosphorylation Source: SGD
  9. pseudohyphal growth Source: SGD
  10. regulation of transposition, RNA-mediated Source: SGD
  11. signal transduction involved in filamentous growth Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Pheromone response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32433-MONOMER.
BRENDAi2.7.11.25. 984.
ReactomeiREACT_188484. Oxidative Stress Induced Senescence.
REACT_191493. FCERI mediated MAPK activation.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase STE11 (EC:2.7.11.25)
Gene namesi
Name:STE11
Ordered Locus Names:YLR362W
ORF Names:L8039.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XII

Organism-specific databases

SGDiS000004354. STE11.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi59 – 591I → R: Disrupts interaction with STE50 and abolishes signal transduction. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 717717Serine/threonine-protein kinase STE11PRO_0000086684Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei323 – 3231Phosphoserine2 Publications
Modified residuei465 – 4651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23561.
PaxDbiP23561.
PeptideAtlasiP23561.

Expressioni

Gene expression databases

GenevestigatoriP23561.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts (via SAM domain) with STE50 (via SAM domain). Interacts with PBS2 and SHO1.3 PublicationsCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-18259,EBI-18259
FUS3P168926EBI-18259,EBI-7193
KSS1P146814EBI-18259,EBI-9945
SHO1P400733EBI-18259,EBI-18140
STE5P329177EBI-18259,EBI-18373
STE50P253446EBI-18259,EBI-18305

Protein-protein interaction databases

BioGridi31623. 108 interactions.
DIPiDIP-861N.
IntActiP23561. 19 interactions.
MINTiMINT-408610.
STRINGi4932.YLR362W.

Structurei

Secondary structure

1
717
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi24 – 329Combined sources
Helixi36 – 449Combined sources
Helixi49 – 546Combined sources
Helixi57 – 637Combined sources
Helixi68 – 8013Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OW5NMR-A15-92[»]
1X9XNMR-A/B16-83[»]
ProteinModelPortaliP23561.
SMRiP23561. Positions 22-83, 394-712.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23561.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 8465SAMPROSITE-ProRule annotationAdd
BLAST
Domaini415 – 712298Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SAM (sterile alpha motif) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119077.
HOGENOMiHOG000172453.
InParanoidiP23561.
KOiK11228.
OMAiVELVTIC.
OrthoDBiEOG71K6B8.

Family and domain databases

Gene3Di1.10.150.50. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR029458. Ras-bd_By2.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF14847. Ras_bdg_2. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view]
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23561-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQTQTAEGT DLLIGDEKTN DLPFVQLFLE EIGCTQYLDS FIQCNLVTEE
60 70 80 90 100
EIKYLDKDIL IALGVNKIGD RLKILRKSKS FQRDKRIEQV NRLKNLMEKV
110 120 130 140 150
SSLSTATLSM NSELIPEKHC VIFILNDGSA KKVNVNGCFN ADSIKKRLIR
160 170 180 190 200
RLPHELLATN SNGEVTKMVQ DYDVFVLDYT KNVLHLLYDV ELVTICHAND
210 220 230 240 250
RVEKNRLIFV SKDQTPSDKA ISTSKKLYLR TLSALSQVGP SSSNLLAQNK
260 270 280 290 300
GISHNNAEGK LRIDNTEKDR IRQIFNQRPP SEFISTNLAG YFPHTDMKRL
310 320 330 340 350
QKTMRESFRH SARLSIAQRR PLSAESNNIG DILLKHSNAV DMALLQGLDQ
360 370 380 390 400
TRLSSKLDTT KIPKLAHKRP EDNDAISNQL ELLSVESGEE EDHDFFGEDS
410 420 430 440 450
DIVSLPTKIA TPKNWLKGAC IGSGSFGSVY LGMNAHTGEL MAVKQVEIKN
460 470 480 490 500
NNIGVPTDNN KQANSDENNE QEEQQEKIED VGAVSHPKTN QNIHRKMVDA
510 520 530 540 550
LQHEMNLLKE LHHENIVTYY GASQEGGNLN IFLEYVPGGS VSSMLNNYGP
560 570 580 590 600
FEESLITNFT RQILIGVAYL HKKNIIHRDI KGANILIDIK GCVKITDFGI
610 620 630 640 650
SKKLSPLNKK QNKRASLQGS VFWMSPEVVK QTATTAKADI WSTGCVVIEM
660 670 680 690 700
FTGKHPFPDF SQMQAIFKIG TNTTPEIPSW ATSEGKNFLR KAFELDYQYR
710
PSALELLQHP WLDAHII
Length:717
Mass (Da):80,721
Last modified:June 16, 2009 - v3
Checksum:iE9E0DF27114EEEEF
GO

Sequence cautioni

The sequence AAB67571.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53431 Genomic DNA. Translation: CAA37522.1.
U19103 Genomic DNA. Translation: AAB67571.1. Different initiation.
BK006945 Genomic DNA. Translation: DAA09666.1.
PIRiS51380.
RefSeqiNP_013466.1. NM_001182251.1.

Genome annotation databases

EnsemblFungiiYLR362W; YLR362W; YLR362W.
GeneIDi851076.
KEGGisce:YLR362W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53431 Genomic DNA. Translation: CAA37522.1 .
U19103 Genomic DNA. Translation: AAB67571.1 . Different initiation.
BK006945 Genomic DNA. Translation: DAA09666.1 .
PIRi S51380.
RefSeqi NP_013466.1. NM_001182251.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1OW5 NMR - A 15-92 [» ]
1X9X NMR - A/B 16-83 [» ]
ProteinModelPortali P23561.
SMRi P23561. Positions 22-83, 394-712.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31623. 108 interactions.
DIPi DIP-861N.
IntActi P23561. 19 interactions.
MINTi MINT-408610.
STRINGi 4932.YLR362W.

Proteomic databases

MaxQBi P23561.
PaxDbi P23561.
PeptideAtlasi P23561.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YLR362W ; YLR362W ; YLR362W .
GeneIDi 851076.
KEGGi sce:YLR362W.

Organism-specific databases

SGDi S000004354. STE11.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119077.
HOGENOMi HOG000172453.
InParanoidi P23561.
KOi K11228.
OMAi VELVTIC.
OrthoDBi EOG71K6B8.

Enzyme and pathway databases

BioCyci YEAST:G3O-32433-MONOMER.
BRENDAi 2.7.11.25. 984.
Reactomei REACT_188484. Oxidative Stress Induced Senescence.
REACT_191493. FCERI mediated MAPK activation.

Miscellaneous databases

EvolutionaryTracei P23561.
NextBioi 967729.

Gene expression databases

Genevestigatori P23561.

Family and domain databases

Gene3Di 1.10.150.50. 1 hit.
InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR029458. Ras-bd_By2.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR011510. SAM_2.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
PF14847. Ras_bdg_2. 1 hit.
PF07647. SAM_2. 1 hit.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
SM00454. SAM. 1 hit.
[Graphical view ]
SUPFAMi SSF47769. SSF47769. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "STE11 is a protein kinase required for cell-type-specific transcription and signal transduction in yeast."
    Rhodes N., Connell L., Errede B.
    Genes Dev. 4:1862-1874(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION OF INITIATION SITE.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII."
    Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W., Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A., Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K., Heuss-Neitzel D., Hilbert H.
    , Hilger F., Kleine K., Koetter P., Louis E.J., Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S., Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D., Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M., Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P., Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M., Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K., Zollner A., Hani J., Hoheisel J.D.
    Nature 387:87-90(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Order of action of components in the yeast pheromone response pathway revealed with a dominant allele of the STE11 kinase and the multiple phosphorylation of the STE7 kinase."
    Cairns B.R., Ramer S.W., Kornberg K.D.
    Genes Dev. 6:1305-1318(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: POSSIBLE FUNCTION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Sho1 and Pbs2 act as coscaffolds linking components in the yeast high osmolarity MAP kinase pathway."
    Zarrinpar A., Bhattacharyya R.P., Nittler M.P., Lim W.A.
    Mol. Cell 14:825-832(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PBS2 AND SHO1.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-465, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The solution structure of the S.cerevisiae Ste11 MAPKKK SAM domain and its partnership with Ste50."
    Kwan J.J., Warner N., Pawson T., Donaldson L.W.
    J. Mol. Biol. 342:681-693(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 15-92, INTERACTION WITH STE50, MUTAGENESIS OF ILE-59.
  11. "Solution structure of the dimeric SAM domain of MAPKKK Ste11 and its interactions with the adaptor protein Ste50 from the budding yeast: implications for Ste11 activation and signal transmission through the Ste50-Ste11 complex."
    Bhattacharjya S., Xu P., Gingras R., Shaykhutdinov R., Wu C., Whiteway M., Ni F.
    J. Mol. Biol. 344:1071-1087(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 16-83, SUBUNIT, INTERACTION WITH STE50.

Entry informationi

Entry nameiSTE11_YEAST
AccessioniPrimary (citable) accession number: P23561
Secondary accession number(s): D6VZ00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 16, 2009
Last modified: November 26, 2014
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

External Data

Dasty 3