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Protein

Serine/threonine-protein kinase STE11

Gene

STE11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine protein kinase required for cell-type-specific transcription and signal transduction in yeast. It is thought that it phosphorylates the STE7 protein kinase which itself, phosphorylates the FUS3 and or KSS1 kinases.1 Publication

Miscellaneous

Present with 736 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei444ATPPROSITE-ProRule annotation1
Active sitei579Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi421 – 429ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • MAP kinase kinase kinase activity Source: SGD
  • SAM domain binding Source: SGD

GO - Biological processi

  • activation of MAPKK activity Source: SGD
  • cellular response to heat Source: SGD
  • invasive growth in response to glucose limitation Source: SGD
  • MAPK cascade involved in cell wall organization or biogenesis Source: SGD
  • MAPK cascade involved in osmosensory signaling pathway Source: SGD
  • osmosensory signaling pathway via Sho1 osmosensor Source: SGD
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: SGD
  • protein phosphorylation Source: SGD
  • pseudohyphal growth Source: SGD
  • regulation of apoptotic process Source: GO_Central
  • regulation of mitotic cell cycle Source: GO_Central
  • regulation of transposition, RNA-mediated Source: SGD
  • signal transduction involved in filamentous growth Source: SGD
  • stress-activated protein kinase signaling cascade Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processPheromone response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-32433-MONOMER.
BRENDAi2.7.11.25. 984.
ReactomeiR-SCE-2559580. Oxidative Stress Induced Senescence.
R-SCE-2871796. FCERI mediated MAPK activation.
R-SCE-389357. CD28 dependent PI3K/Akt signaling.
R-SCE-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-SCE-5668541. TNFR2 non-canonical NF-kB pathway.
R-SCE-5676590. NIK-->noncanonical NF-kB signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase STE11 (EC:2.7.11.25)
Gene namesi
Name:STE11
Ordered Locus Names:YLR362W
ORF Names:L8039.10
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XII

Organism-specific databases

EuPathDBiFungiDB:YLR362W.
SGDiS000004354. STE11.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi59I → R: Disrupts interaction with STE50 and abolishes signal transduction. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866841 – 717Serine/threonine-protein kinase STE11Add BLAST717

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei323PhosphoserineCombined sources1
Modified residuei465PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23561.
PRIDEiP23561.

PTM databases

iPTMnetiP23561.

Interactioni

Subunit structurei

Homodimer (Probable). Interacts (via SAM domain) with STE50 (via SAM domain). Interacts with PBS2 and SHO1.Curated3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • SAM domain binding Source: SGD

Protein-protein interaction databases

BioGridi31623. 114 interactors.
DIPiDIP-861N.
IntActiP23561. 22 interactors.
MINTiMINT-408610.
STRINGi4932.YLR362W.

Structurei

Secondary structure

1717
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi24 – 32Combined sources9
Helixi36 – 44Combined sources9
Helixi49 – 54Combined sources6
Helixi57 – 63Combined sources7
Helixi68 – 80Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OW5NMR-A15-92[»]
1X9XNMR-A/B16-83[»]
ProteinModelPortaliP23561.
SMRiP23561.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23561.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 84SAMPROSITE-ProRule annotationAdd BLAST65
Domaini415 – 712Protein kinasePROSITE-ProRule annotationAdd BLAST298

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00800000124036.
HOGENOMiHOG000172453.
InParanoidiP23561.
KOiK11228.
OMAiHGEDIIH.
OrthoDBiEOG092C030N.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR029458. Ras-bd_By2.
IPR001660. SAM.
IPR013761. SAM/pointed.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PF14847. Ras_bdg_2. 1 hit.
PF07647. SAM_2. 1 hit.
SMARTiView protein in SMART
SM01304. Ras_bdg_2. 1 hit.
SM00220. S_TKc. 1 hit.
SM00454. SAM. 1 hit.
SUPFAMiSSF47769. SSF47769. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiView protein in PROSITE
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50105. SAM_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P23561-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEQTQTAEGT DLLIGDEKTN DLPFVQLFLE EIGCTQYLDS FIQCNLVTEE
60 70 80 90 100
EIKYLDKDIL IALGVNKIGD RLKILRKSKS FQRDKRIEQV NRLKNLMEKV
110 120 130 140 150
SSLSTATLSM NSELIPEKHC VIFILNDGSA KKVNVNGCFN ADSIKKRLIR
160 170 180 190 200
RLPHELLATN SNGEVTKMVQ DYDVFVLDYT KNVLHLLYDV ELVTICHAND
210 220 230 240 250
RVEKNRLIFV SKDQTPSDKA ISTSKKLYLR TLSALSQVGP SSSNLLAQNK
260 270 280 290 300
GISHNNAEGK LRIDNTEKDR IRQIFNQRPP SEFISTNLAG YFPHTDMKRL
310 320 330 340 350
QKTMRESFRH SARLSIAQRR PLSAESNNIG DILLKHSNAV DMALLQGLDQ
360 370 380 390 400
TRLSSKLDTT KIPKLAHKRP EDNDAISNQL ELLSVESGEE EDHDFFGEDS
410 420 430 440 450
DIVSLPTKIA TPKNWLKGAC IGSGSFGSVY LGMNAHTGEL MAVKQVEIKN
460 470 480 490 500
NNIGVPTDNN KQANSDENNE QEEQQEKIED VGAVSHPKTN QNIHRKMVDA
510 520 530 540 550
LQHEMNLLKE LHHENIVTYY GASQEGGNLN IFLEYVPGGS VSSMLNNYGP
560 570 580 590 600
FEESLITNFT RQILIGVAYL HKKNIIHRDI KGANILIDIK GCVKITDFGI
610 620 630 640 650
SKKLSPLNKK QNKRASLQGS VFWMSPEVVK QTATTAKADI WSTGCVVIEM
660 670 680 690 700
FTGKHPFPDF SQMQAIFKIG TNTTPEIPSW ATSEGKNFLR KAFELDYQYR
710
PSALELLQHP WLDAHII
Length:717
Mass (Da):80,721
Last modified:June 16, 2009 - v3
Checksum:iE9E0DF27114EEEEF
GO

Sequence cautioni

The sequence AAB67571 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53431 Genomic DNA. Translation: CAA37522.1.
U19103 Genomic DNA. Translation: AAB67571.1. Different initiation.
BK006945 Genomic DNA. Translation: DAA09666.1.
PIRiS51380.
RefSeqiNP_013466.1. NM_001182251.1.

Genome annotation databases

EnsemblFungiiYLR362W; YLR362W; YLR362W.
GeneIDi851076.
KEGGisce:YLR362W.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSTE11_YEAST
AccessioniPrimary (citable) accession number: P23561
Secondary accession number(s): D6VZ00
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: June 16, 2009
Last modified: July 5, 2017
This is version 180 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XII
    Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names