Reviewed, UniProtKB/Swiss-Prot P23557 (XYN4_CALSA)

Names and origin

Protein names	Recommended name:     Putative endo-1,4-beta-xylanase       Short name=Xylanase     EC=3.2.1.8 Alternative name(s):     1,4-beta-D-xylan xylanohydrolase     ORF4
Organism	Caldocellum saccharolyticum (Caldicellulosiruptor saccharolyticus)
Taxonomic identifier	44001 [NCBI]
Taxonomic lineage	Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacterales Family III. Incertae Sedis › Caldicellulosiruptor

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Could be a xylanase By similarity.
Catalytic activity	Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans.
Pathway	Glycan degradation; xylan degradation.
Sequence similarities	Belongs to the glycosyl hydrolase 10 (cellulase F) family.

Ontologies

Keywords
Biological process	Xylan degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	xylan catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro endo-1,4-beta-xylanase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Putative endo-1,4-beta-xylanase		PRO_0000184068
Regions
	Region	23 – 274	252	Catalytic Potential
Sites
	Active site	104	1	Proton donor By similarity
	Active site	216	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23557-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 6C2A049212F9CB6E	FASTA	312	36,494
	10 20 30 40 50 60 MKQQYLLDYE ATKASKNGMP VCKFDSCIPA LQFCKENGIK MRGHVLVWHN QTPEWFFHKD 70 80 90 100 110 120 YDVSKPLVDA ATMERRLESY IKQVIEFCQK NYPGVVYCWD VVNEAILDDG SWREINNNWY 130 140 150 160 170 180 TIMKEKYVEK AFYYARKYAK KDVALFYNDY NVFLPAKREA IYNLAQKLKE KGLIDGLGLQ 190 200 210 220 230 240 PTVGLNYPEL DSDDIDSFKT TLETFAKLGL QIHITELNFE IKGDESNRTP ENLKKQADRY 250 260 270 280 290 300 YEMMKLLLKE DTDNGGPCNI TCVTVFGICD DYPLYKNFKQ CMYLWDKNCN PKPCFYSFLQ 310 AGLDWKASLL SK

References

[1]

"Cloning, sequence analysis, and expression of genes encoding xylan-degrading enzymes from the thermophile 'Caldocellum saccharolyticum'." Luethi E., Love D.R., McAnulty J., Wallace C., Caughey P.A., Saul D.J., Bergquist P.L. Appl. Environ. Microbiol. 56:1017-1024(1990) [PubMed: 2111111] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M34459 Genomic DNA. Translation: AAA23062.1.
PIR	D37202.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH10. Glycoside Hydrolase Family 10.
Enzyme and pathway databases
BRENDA	3.2.1.8. 15230.
Family and domain databases
InterPro	IPR001000. Glyco_hydro_10. IPR017853. Glyco_hydro_catalytic_core. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00331. Glyco_hydro_10. 1 hit. [Graphical view]
PRINTS	PR00134. GLHYDRLASE10.
SMART	SM00633. Glyco_10. 1 hit. [Graphical view]
PROSITE	PS00591. GLYCOSYL_HYDROL_F10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYN4_CALSA

Accession

Primary (citable) accession number: P23557

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	November 24, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)