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P23549 (GUN3_BACIU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Endoglucanase
EC=3.2.1.4
Alternative name(s):
Carboxymethyl-cellulase
Short name=CMCase
Short name=Cellulase
Endo-1,4-beta-glucanase
Gene names
Name:bglC
OrganismBacillus subtilis
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus ›

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Sequence similarities

Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Contains 1 CBM3 (carbohydrate binding type-3) domain.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Cellulose degradation
Polysaccharide degradation
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
Gene Ontology (GO)
   Biological_processcellulose catabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioncellulase activity

Inferred from electronic annotation. Source: EC

cellulose binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929
Chain30 – 499470Endoglucanase
PRO_0000007841

Regions

Domain350 – 499150CBM3

Sites

Active site1691Proton donor By similarity
Active site2571Nucleophile By similarity

Sequences

Sequence LengthMass (Da)Tools
P23549 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 2E821E3D8BBACA04

FASTA49955,169
        10         20         30         40         50         60 
MKRSISIFIT CLLITLLTMG GMLASPASAA GTKTPVAKNG QLSIKGTQLV NRDGKAVQLK 

        70         80         90        100        110        120 
GISSHGLQWY GEYVNKDSLK WLRDDWGITV FRAAMYTADG GIIDNPSVKN KMKEAVEAAK 

       130        140        150        160        170        180 
ELGIYVIIDW HILNDGNPNQ NKEKAKEFFK EMSSLYGNTP NVIYEIANEP NGDVNWKRDI 

       190        200        210        220        230        240 
KPYAEEVISV IRKNDPDNII IVGTGTWSQD VNDAADDQLK DANVMDALHF YAGTHGQFLR 

       250        260        270        280        290        300 
DKANYALSKG APIFVTEWGT SDASGNGGVF LDQSREWLKY LDSKTISWVN WNLSDKQESS 

       310        320        330        340        350        360 
SALKPGASKT GGWRLSDLSA SGTFVRENIL GTKDSTKDIP ETPAKDKPTQ ENGISVQYRA 

       370        380        390        400        410        420 
GDGSMNSNQI RPQLQIKNNG NTTVDLKDVT ARYWYNAKNK GQNVDCDYAQ LGCGNVTYKF 

       430        440        450        460        470        480 
VTLHKPKQGA DTYLELGFKN GTLAPGASTG NIQLRLHNDD WSNYAQSGDY SFFKSNTFKT 

       490 
TKKITLYDQG KLIWGTEPN

« Hide

References

[1]"Characterization and structure of the cellulase gene of Bacillus subtilis BSE616."
Park S.H., Kim H.K., Pack M.Y.
Agric. Biol. Chem. 55:441-448(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BSE616.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D01057 Genomic DNA. Translation: BAA00859.1.
PIRJN0111.

3D structure databases

ProteinModelPortalP23549.
SMRP23549. Positions 36-329.
ModBaseSearch...

Protein family/group databases

CAZyCBM3. Carbohydrate-Binding Module Family 3.
GH5. Glycoside Hydrolase Family 5.

Proteomic databases

PaxDbP23549.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG2730.

Family and domain databases

Gene3D2.60.40.710. 1 hit.
3.20.20.80. 1 hit.
InterProIPR008965. Carb-bd_dom.
IPR001956. CBD_3.
IPR001547. Glyco_hydro_5.
IPR018087. Glyco_hydro_5_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00942. CBM_3. 1 hit.
PF00150. Cellulase. 1 hit.
[Graphical view]
SMARTSM01067. CBM_3. 1 hit.
[Graphical view]
SUPFAMSSF49384. Cellul_bind. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS51172. CBM3. 1 hit.
PS00659. GLYCOSYL_HYDROL_F5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGUN3_BACIU
AccessionPrimary (citable) accession number: P23549
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 1, 2013
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

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