P23548 (GUN_PAEPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Endoglucanase EC=3.2.1.4 Alternative name(s): Cellulase Endo-1,4-beta-glucanase
Organism	Paenibacillus polymyxa (Bacillus polymyxa)
Taxonomic identifier	1406 [NCBI]
Taxonomic lineage	cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Paenibacillaceae › Paenibacillus

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 5 (cellulase A) family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Cellulose degradation Polysaccharide degradation
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological_process	cellulose catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cellulase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Endoglucanase		PRO_0000184042
Sites
	Active site	194	1	Proton donor By similarity
	Active site	317	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23548 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: B9C2E802C04F0A2A	FASTA	397	44,357
	10 20 30 40 50 60 MKKKGLKKTF FVIASLVMGF TLYGYTPVSA DAASVKGYYH TQGNKIVDES GKEAAFNGLN 70 80 90 100 110 120 WFGLETPNYT LHGLWSRSMD DMLDQVKKEG YNLIRLPYSN QLFDSSSRPD SIDYHKNPDL 130 140 150 160 170 180 VGLNPIQIMD KLIEKAGQRG IQIILDRHRP GSGGQSELWY TSQYPESRWI SDWKMLADRY 190 200 210 220 230 240 KNNPTVIGAD LHNEPHGQAS WGTGNASTDW RLAAQRAGNA ILSVNPNWLI LVEGVDHNVQ 250 260 270 280 290 300 GNNSQYWWGG NLTGVANYPV VLDVPNRVVY SPHDYGPGVS SQPWFNDPAF PSNLPAIWDQ 310 320 330 340 350 360 TWGYISKQNI APVLVGEFGG RNVDLSCPEG KWQNALVHYI GANNLYFTYW SLNPNSGDTG 370 380 390 GLLLDDWTTW NRPKQDMLGR IMKPVVSVAQ QAEAAAE

References

[1]	"Molecular cloning, expression, and characterization of endo-beta-1,4-glucanase genes from Bacillus polymyxa and Bacillus circulans." Baird S.D., Johnson D.A., Seligy V.L. J. Bacteriol. 172:1576-1586(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M33791 Genomic DNA. Translation: AAA22631.1.
PIR	A35136.
3D structure databases
ProteinModelPortal	P23548.
SMR	P23548. Positions 34-380.
ModBase	Search...
Protein family/group databases
CAZy	GH5. Glycoside Hydrolase Family 5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001547. Glyco_hydro_5. IPR018087. Glyco_hydro_5_CS. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00150. Cellulase. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00659. GLYCOSYL_HYDROL_F5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUN_PAEPO

Accession

Primary (citable) accession number: P23548

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program