P23547 (E13G_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GI9
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
PR-2B
PR-36

Gene names

Name:

PR2

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	343 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space.
Induction	Not found in healthy tissues, but accumulates to high levels in the extracellular compartment of leaves in response to pathogen infection or treatment with salicylic acid.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Probable
	Chain	30 – 343	314	Glucan endo-1,3-beta-glucosidase, acidic isoform GI9		PRO_0000011877
Sites
	Active site	264	1	Nucleophile By similarity
	Active site	324	1	Proton donor By similarity
Amino acid modifications
	Modified residue	30	1	Pyrrolidone carboxylic acid Probable
Experimental info
	Sequence conflict	54	1	N → D in AAA34103. Ref.2
	Sequence conflict	172	1	Y → N in AAA34103. Ref.2
	Sequence conflict	303	1	K → N in AAA34103. Ref.2
	Sequence conflict	323	1	Missing AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23547 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 22854FFAFE6B97E3	FASTA	343	37,723
	10 20 30 40 50 60 MTLCIKNGFL AAALVLVGLL ICSIQMIGAQ SIGVCYGKHA NNLPSDQDVI NLYNANGIRK 70 80 90 100 110 120 MRIYNPDTNV FNALRGSNIE IILDVPLQDL QSLTDPSRAN GWVQDNIINH FPDVKFKYIA 130 140 150 160 170 180 VGNEVSPGNN GQYAPFVAPA MQNVYNALAA AGLQDQIKVS TATYSGILAN TYPPKDSIFR 190 200 210 220 230 240 GEFNSFINPI IQFLVQHNLP LLANVYPYFG HIFNTADVPL SYALFTQQEA NPAGYQNLFD 250 260 270 280 290 300 ALLDSMYFAV EKAGGQNVEI IVSESGWPSE GNSAATIENA QTYYENLINH VKSGAGTPKK 310 320 330 340 PGKAIETYLF AMFDENNKEG DITEKHFGLF SPDQRAKYQL NFN

References

[1]	"Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco." Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F. Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Samsun NN.
[2]	"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection." Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A. Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Xanthi NC. Tissue: Leaf.
[3]	"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system." van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J. Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 39-115; 157-158; 253-276 AND 338-343.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59443 Genomic DNA. Translation: AAA63542.1. M60460 mRNA. Translation: AAA34103.1.
PIR	B38257. C38257.
3D structure databases
ProteinModelPortal	P23547.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13G_TOBAC

Accession

Primary (citable) accession number: P23547

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program