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P23547

- E13G_TOBAC

UniProt

P23547 - E13G_TOBAC

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Protein
Glucan endo-1,3-beta-glucosidase, acidic isoform GI9
Gene
PR2
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei264 – 2641Nucleophile By similarity
Active sitei324 – 3241Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GI9 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
PR-2B
PR-36
Gene namesi
Name:PR2
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Inferred
Add
BLAST
Chaini30 – 343314Glucan endo-1,3-beta-glucosidase, acidic isoform GI9
PRO_0000011877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Pyrrolidone carboxylic acid Inferred

Keywords - PTMi

Pyrrolidone carboxylic acid

Proteomic databases

PRIDEiP23547.

Expressioni

Inductioni

Not found in healthy tissues, but accumulates to high levels in the extracellular compartment of leaves in response to pathogen infection or treatment with salicylic acid.

Structurei

3D structure databases

ProteinModelPortaliP23547.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23547-1 [UniParc]FASTAAdd to Basket

« Hide

MTLCIKNGFL AAALVLVGLL ICSIQMIGAQ SIGVCYGKHA NNLPSDQDVI    50
NLYNANGIRK MRIYNPDTNV FNALRGSNIE IILDVPLQDL QSLTDPSRAN 100
GWVQDNIINH FPDVKFKYIA VGNEVSPGNN GQYAPFVAPA MQNVYNALAA 150
AGLQDQIKVS TATYSGILAN TYPPKDSIFR GEFNSFINPI IQFLVQHNLP 200
LLANVYPYFG HIFNTADVPL SYALFTQQEA NPAGYQNLFD ALLDSMYFAV 250
EKAGGQNVEI IVSESGWPSE GNSAATIENA QTYYENLINH VKSGAGTPKK 300
PGKAIETYLF AMFDENNKEG DITEKHFGLF SPDQRAKYQL NFN 343
Length:343
Mass (Da):37,723
Last modified:November 1, 1991 - v1
Checksum:i22854FFAFE6B97E3
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti54 – 541N → D in AAA34103. 1 Publication
Sequence conflicti172 – 1721Y → N in AAA34103. 1 Publication
Sequence conflicti303 – 3031K → N in AAA34103. 1 Publication
Sequence conflicti323 – 3231Missing AA sequence 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59443 Genomic DNA. Translation: AAA63542.1.
M60460 mRNA. Translation: AAA34103.1.
PIRiB38257.
C38257.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59443 Genomic DNA. Translation: AAA63542.1 .
M60460 mRNA. Translation: AAA34103.1 .
PIRi B38257.
C38257.

3D structure databases

ProteinModelPortali P23547.
ModBasei Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Proteomic databases

PRIDEi P23547.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco."
    Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F.
    Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Samsun NN.
  2. "Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection."
    Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.
    Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Strain: cv. Xanthi NC.
    Tissue: Leaf.
  3. "Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
    van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
    Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 39-115; 157-158; 253-276 AND 338-343.

Entry informationi

Entry nameiE13G_TOBAC
AccessioniPrimary (citable) accession number: P23547
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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