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P23547 (E13G_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GI9

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
PR-2B
PR-36
Gene names
Name:PR2
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length343 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the defense of plants against pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedextracellular space.

Induction

Not found in healthy tissues, but accumulates to high levels in the extracellular compartment of leaves in response to pathogen infection or treatment with salicylic acid.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMPyrrolidone carboxylic acid
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Probable
Chain30 – 343314Glucan endo-1,3-beta-glucosidase, acidic isoform GI9
PRO_0000011877

Sites

Active site2641Nucleophile By similarity
Active site3241Proton donor By similarity

Amino acid modifications

Modified residue301Pyrrolidone carboxylic acid Probable

Experimental info

Sequence conflict541N → D in AAA34103. Ref.2
Sequence conflict1721Y → N in AAA34103. Ref.2
Sequence conflict3031K → N in AAA34103. Ref.2
Sequence conflict3231Missing AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P23547 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 22854FFAFE6B97E3

FASTA34337,723
        10         20         30         40         50         60 
MTLCIKNGFL AAALVLVGLL ICSIQMIGAQ SIGVCYGKHA NNLPSDQDVI NLYNANGIRK 

        70         80         90        100        110        120 
MRIYNPDTNV FNALRGSNIE IILDVPLQDL QSLTDPSRAN GWVQDNIINH FPDVKFKYIA 

       130        140        150        160        170        180 
VGNEVSPGNN GQYAPFVAPA MQNVYNALAA AGLQDQIKVS TATYSGILAN TYPPKDSIFR 

       190        200        210        220        230        240 
GEFNSFINPI IQFLVQHNLP LLANVYPYFG HIFNTADVPL SYALFTQQEA NPAGYQNLFD 

       250        260        270        280        290        300 
ALLDSMYFAV EKAGGQNVEI IVSESGWPSE GNSAATIENA QTYYENLINH VKSGAGTPKK 

       310        320        330        340 
PGKAIETYLF AMFDENNKEG DITEKHFGLF SPDQRAKYQL NFN 

« Hide

References

[1]"Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco."
Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F.
Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: cv. Samsun NN.
[2]"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection."
Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.
Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Strain: cv. Xanthi NC.
Tissue: Leaf.
[3]"Characterization of vacuolar and extracellular beta(1,3)-glucanases of tobacco: evidence for a strictly compartmentalized plant defense system."
van den Bulcke M., Bauw G., Castresana C., van Montagu M., Vandekerckhove J.
Proc. Natl. Acad. Sci. U.S.A. 86:2673-2677(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 39-115; 157-158; 253-276 AND 338-343.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M59443 Genomic DNA. Translation: AAA63542.1.
M60460 mRNA. Translation: AAA34103.1.
PIRB38257.
C38257.

3D structure databases

ProteinModelPortalP23547.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Proteomic databases

PRIDEP23547.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13G_TOBAC
AccessionPrimary (citable) accession number: P23547
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries