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Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731NucleophileBy similarity
Active sitei329 – 3291Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLA
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Chaini33 – 348316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50PRO_0000011871Add
BLAST
Propeptidei349 – 37022Removed in mature formBy similarityPRO_0000011872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acidCurated
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By ethylene and salicylic acid.

Structurei

3D structure databases

ProteinModelPortaliP23546.
SMRiP23546. Positions 35-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23546-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSHKHNTP QMAAITLLGL LLVASSIDIA GAQSIGVCYG MLGNNLPNHW
60 70 80 90 100
EVIQLYKSRN IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM
110 120 130 140 150
EHARWWVQKN VKDFWPDVKI KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY
160 170 180 190 200
KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS QGSFRNDARW FVDAIVGFLR
210 220 230 240 250
DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG SRQYRNLFDA
260 270 280 290 300
MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA
310 320 330 340 350
KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNINFGVS
360 370
GGVWDSSVET NATASLVSEM
Length:370
Mass (Da):40,395
Last modified:November 1, 1991 - v1
Checksum:i561AE5662478D7A9
GO

Sequence cautioni

The sequence AAA63541.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941A → P (PubMed:2103484).Curated
Sequence conflicti194 – 1941A → P (PubMed:2000389).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59442 Genomic DNA. Translation: AAA63541.1. Different initiation.
X53600 Genomic DNA. Translation: CAA37669.1.
M60402 Genomic DNA. Translation: AAA63539.1.
S44870 mRNA. Translation: AAB23376.1.
PIRiA39115.
S12406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M59442 Genomic DNA. Translation: AAA63541.1. Different initiation.
X53600 Genomic DNA. Translation: CAA37669.1.
M60402 Genomic DNA. Translation: AAA63539.1.
S44870 mRNA. Translation: AAB23376.1.
PIRiA39115.
S12406.

3D structure databases

ProteinModelPortaliP23546.
SMRiP23546. Positions 35-347.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco."
    Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F.
    Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Samsun NN.
  2. "Structure and expression of a tobacco beta-1,3-glucanase gene."
    Ohme-Takagi M., Shinshi H.
    Plant Mol. Biol. 15:941-946(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Bright Yellow 4.
  3. "Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family."
    Sperisen C., Ryals J., Meins F.
    Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation."
    Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A., Meeks-Wagner D.R., Peacock W.J., Dennis E.S.
    Plant Cell 2:673-684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 314-370.
  5. "Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes."
    Bauw G., Rasmussen H.H., van den Bulcke M., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
    Electrophoresis 11:528-536(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 66-78; 105-109 AND 343-348.

Entry informationi

Entry nameiE13E_TOBAC
AccessioniPrimary (citable) accession number: P23546
Secondary accession number(s): Q9SAT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: January 7, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.