P23546 (E13E_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50 EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase Short name=(1->3)-beta-glucanase Beta-1,3-endoglucanase, basic Glucanase GLA
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole.
Tissue specificity	Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.
Induction	By ethylene and salicylic acid.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.
Sequence caution	The sequence AAA63541.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32
	Chain	33 – 348	316	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50		PRO_0000011871
	Propeptide	349 – 370	22	Removed in mature form By similarity		PRO_0000011872
Sites
	Active site	273	1	Nucleophile By similarity
	Active site	329	1	Proton donor By similarity
Amino acid modifications
	Modified residue	33	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	194	1	A → P Ref.2
	Sequence conflict	194	1	A → P Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P23546 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 561AE5662478D7A9	FASTA	370	40,395
	10 20 30 40 50 60 MSTSHKHNTP QMAAITLLGL LLVASSIDIA GAQSIGVCYG MLGNNLPNHW EVIQLYKSRN 70 80 90 100 110 120 IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI 130 140 150 160 170 180 KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS 190 200 210 220 230 240 QGSFRNDARW FVDAIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG 250 260 270 280 290 300 SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA 310 320 330 340 350 360 KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNINFGVS GGVWDSSVET 370 NATASLVSEM

References

[1]	"Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco." Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F. Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Samsun NN.
[2]	"Structure and expression of a tobacco beta-1,3-glucanase gene." Ohme-Takagi M., Shinshi H. Plant Mol. Biol. 15:941-946(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Bright Yellow 4.
[3]	"Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family." Sperisen C., Ryals J., Meins F. Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE.
[4]	"Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation." Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A., Meeks-Wagner D.R., Peacock W.J., Dennis E.S. Plant Cell 2:673-684(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 314-370.
[5]	"Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes." Bauw G., Rasmussen H.H., van den Bulcke M., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J. Electrophoresis 11:528-536(1990) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 66-78; 105-109 AND 343-348.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M59442 Genomic DNA. Translation: AAA63541.1. Different initiation. X53600 Genomic DNA. Translation: CAA37669.1. M60402 Genomic DNA. Translation: AAA63539.1. S44870 mRNA. Translation: AAB23376.1.
PIR	A39115. S12406.
3D structure databases
ProteinModelPortal	P23546.
SMR	P23546. Positions 35-347.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13E_TOBAC

Accession

Primary (citable) accession number: P23546
Secondary accession number(s): Q9SAT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program