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P23546

- E13E_TOBAC

UniProt

P23546 - E13E_TOBAC

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Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731Nucleophile By similarity
Active sitei329 – 3291Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLA
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Chaini33 – 348316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GGIB50PRO_0000011871Add
BLAST
Propeptidei349 – 37022Removed in mature form By similarityPRO_0000011872Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acid Inferred
Glycosylationi361 – 3611N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By ethylene and salicylic acid.

Structurei

3D structure databases

ProteinModelPortaliP23546.
SMRiP23546. Positions 35-347.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23546-1 [UniParc]FASTAAdd to Basket

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MSTSHKHNTP QMAAITLLGL LLVASSIDIA GAQSIGVCYG MLGNNLPNHW    50
EVIQLYKSRN IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM 100
EHARWWVQKN VKDFWPDVKI KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY 150
KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS QGSFRNDARW FVDAIVGFLR 200
DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG SRQYRNLFDA 250
MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA 300
KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNINFGVS 350
GGVWDSSVET NATASLVSEM 370
Length:370
Mass (Da):40,395
Last modified:November 1, 1991 - v1
Checksum:i561AE5662478D7A9
GO

Sequence cautioni

The sequence AAA63541.1 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti194 – 1941A → P1 Publication
Sequence conflicti194 – 1941A → P1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59442 Genomic DNA. Translation: AAA63541.1. Different initiation.
X53600 Genomic DNA. Translation: CAA37669.1.
M60402 Genomic DNA. Translation: AAA63539.1.
S44870 mRNA. Translation: AAB23376.1.
PIRiA39115.
S12406.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M59442 Genomic DNA. Translation: AAA63541.1 . Different initiation.
X53600 Genomic DNA. Translation: CAA37669.1 .
M60402 Genomic DNA. Translation: AAA63539.1 .
S44870 mRNA. Translation: AAB23376.1 .
PIRi A39115.
S12406.

3D structure databases

ProteinModelPortali P23546.
SMRi P23546. Positions 35-347.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Analysis of gene families encoding acidic and basic beta-1,3-glucanases of tobacco."
    Linthorst H.J.M., Melchers L.S., Mayer A., van Roekel J.S.C., Cornelissen B.J.C., Bol J.F.
    Proc. Natl. Acad. Sci. U.S.A. 87:8756-8760(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Samsun NN.
  2. "Structure and expression of a tobacco beta-1,3-glucanase gene."
    Ohme-Takagi M., Shinshi H.
    Plant Mol. Biol. 15:941-946(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Bright Yellow 4.
  3. "Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family."
    Sperisen C., Ryals J., Meins F.
    Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
  4. "Chitinase, beta-1,3-glucanase, osmotin, and extensin are expressed in tobacco explants during flower formation."
    Neale A.D., Wahleithner J.A., Lund M., Bonnett H.T., Kelly A., Meeks-Wagner D.R., Peacock W.J., Dennis E.S.
    Plant Cell 2:673-684(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 314-370.
  5. "Two-dimensional gel electrophoresis, protein electroblotting and microsequencing: a direct link between proteins and genes."
    Bauw G., Rasmussen H.H., van den Bulcke M., van Damme J., Puype M., Gesser B., Celis J.E., Vandekerckhove J.
    Electrophoresis 11:528-536(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 66-78; 105-109 AND 343-348.

Entry informationi

Entry nameiE13E_TOBAC
AccessioniPrimary (citable) accession number: P23546
Secondary accession number(s): Q9SAT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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