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Protein

Alkaline phosphatase synthesis sensor protein PhoR

Gene

phoR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system PhoP/PhoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoP in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphate transport, Transport, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU29100-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase synthesis sensor protein PhoR (EC:2.7.13.3)
Gene namesi
Name:phoR
Ordered Locus Names:BSU29100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence analysis
Transmembranei8 – 2821HelicalSequence analysisAdd
BLAST
Topological domaini29 – 150122ExtracellularSequence analysisAdd
BLAST
Transmembranei151 – 17121HelicalSequence analysisAdd
BLAST
Topological domaini172 – 579408CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 579579Alkaline phosphatase synthesis sensor protein PhoRPRO_0000074845Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei360 – 3601Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23545.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015881.

Structurei

Secondary structure

1
579
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi35 – 5319Combined sources
Helixi56 – 583Combined sources
Helixi62 – 7514Combined sources
Beta strandi78 – 836Combined sources
Beta strandi88 – 914Combined sources
Helixi99 – 1068Combined sources
Beta strandi121 – 1288Combined sources
Beta strandi131 – 1399Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CWFX-ray2.20A/B32-150[»]
ProteinModelPortaliP23545.
SMRiP23545. Positions 33-141, 174-226, 236-577.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23545.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini234 – 30067PASAdd
BLAST
Domaini357 – 575219Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG2202. LUCA.
HOGENOMiHOG000223176.
InParanoidiP23545.
KOiK07636.
OMAiVFHDMTE.
OrthoDBiEOG6G4VQG.
PhylomeDBiP23545.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR031967. PhoR_N.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PF16736. sCache_like. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKYRVRLFS VFVVCMILVF CVLGLFLQQL FETSDQRKAE EHIEKEAKYL
60 70 80 90 100
ASLLDAGNLN NQANEKIIKD AGGALDVSAS VIDTDGKVLY GSNGRSADSQ
110 120 130 140 150
KVQALVSGHE GILSTTDNKL YYGLSLRSEG EKTGYVLLSA SEKSDGLKGE
160 170 180 190 200
LWGMLTASLC TAFIVIVYFY SSMTSRYKRS IESATNVATE LSKGNYDART
210 220 230 240 250
YGGYIRRSDK LGHAMNSLAI DLMEMTRTQE MQRDRLLTVI ENIGSGLIMI
260 270 280 290 300
DGRGFINLVN RSYAKQFHIN PNHMLRRLYH DAFEHEEVIQ LVEDIFMTET
310 320 330 340 350
KKCKLLRLPI KIERRYFEVD GVPIMGPDDE WKGIVLVFHD MTETKKLEQM
360 370 380 390 400
RKDFVANVSH ELKTPITSIK GFTETLLDGA MEDKEALSEF LSIILKESER
410 420 430 440 450
LQSLVQDLLD LSKIEQQNFT LSIETFEPAK MLGEIETLLK HKADEKGISL
460 470 480 490 500
HLNVPKDPQY VSGDPYRLKQ VFLNLVNNAL TYTPEGGSVA INVKPREKDI
510 520 530 540 550
QIEVADSGIG IQKEEIPRIF ERFYRVDKDR SRNSGGTGLG LAIVKHLIEA
560 570
HEGKIDVTSE LGRGTVFTVT LKRAAEKSA
Length:579
Mass (Da):65,122
Last modified:November 1, 1991 - v1
Checksum:i0C4EE530A79A477B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23549 Genomic DNA. Translation: AAA22663.1.
AF008220 Genomic DNA. Translation: AAC00349.1.
AL009126 Genomic DNA. Translation: CAB14870.1.
PIRiA27650.
RefSeqiNP_390788.1. NC_000964.3.
WP_004398493.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14870; CAB14870; BSU29100.
GeneIDi937387.
KEGGibsu:BSU29100.
PATRICi18977712. VBIBacSub10457_3044.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23549 Genomic DNA. Translation: AAA22663.1.
AF008220 Genomic DNA. Translation: AAC00349.1.
AL009126 Genomic DNA. Translation: CAB14870.1.
PIRiA27650.
RefSeqiNP_390788.1. NC_000964.3.
WP_004398493.1. NZ_JNCM01000036.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3CWFX-ray2.20A/B32-150[»]
ProteinModelPortaliP23545.
SMRiP23545. Positions 33-141, 174-226, 236-577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015881.

Proteomic databases

PaxDbiP23545.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB14870; CAB14870; BSU29100.
GeneIDi937387.
KEGGibsu:BSU29100.
PATRICi18977712. VBIBacSub10457_3044.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG2202. LUCA.
HOGENOMiHOG000223176.
InParanoidiP23545.
KOiK07636.
OMAiVFHDMTE.
OrthoDBiEOG6G4VQG.
PhylomeDBiP23545.

Enzyme and pathway databases

BioCyciBSUB:BSU29100-MONOMER.
BRENDAi2.7.13.3. 658.

Miscellaneous databases

EvolutionaryTraceiP23545.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR031967. PhoR_N.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PF16736. sCache_like. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the Bacillus subtilis phoR gene."
    Seki T., Yoshikawa H., Takahashi H., Saito H.
    J. Bacteriol. 170:5935-5938(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region."
    Lapidus A., Galleron N., Sorokin A., Ehrlich S.D.
    Microbiology 143:3431-3441(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiPHOR_BACSU
AccessioniPrimary (citable) accession number: P23545
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 11, 2016
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.