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Protein

Alkaline phosphatase synthesis sensor protein PhoR

Gene

phoR

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Member of the two-component regulatory system PhoP/PhoR involved in the alkaline phosphatase genes regulation. PhoR may function as a membrane-associated protein kinase that phosphorylates PhoP in response to environmental signals.

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphate transport, Transport, Two-component regulatory system
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU29100-MONOMER.
BRENDAi2.7.13.3. 658.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase synthesis sensor protein PhoR (EC:2.7.13.3)
Gene namesi
Name:phoR
Ordered Locus Names:BSU29100
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 7CytoplasmicSequence analysis7
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Topological domaini29 – 150ExtracellularSequence analysisAdd BLAST122
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 579CytoplasmicSequence analysisAdd BLAST408

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000748451 – 579Alkaline phosphatase synthesis sensor protein PhoRAdd BLAST579

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei360Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP23545.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100015881.

Structurei

Secondary structure

1579
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi35 – 53Combined sources19
Helixi56 – 58Combined sources3
Helixi62 – 75Combined sources14
Beta strandi78 – 83Combined sources6
Beta strandi88 – 91Combined sources4
Helixi99 – 106Combined sources8
Beta strandi121 – 128Combined sources8
Beta strandi131 – 139Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3CWFX-ray2.20A/B32-150[»]
ProteinModelPortaliP23545.
SMRiP23545.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23545.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini234 – 300PASAdd BLAST67
Domaini357 – 575Histidine kinasePROSITE-ProRule annotationAdd BLAST219

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
COG0642. LUCA.
COG2202. LUCA.
HOGENOMiHOG000223176.
InParanoidiP23545.
KOiK07636.
OMAiVFHDMTE.
PhylomeDBiP23545.

Family and domain databases

CDDicd00075. HATPase_c. 1 hit.
cd00082. HisKA. 1 hit.
cd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003594. HATPase_C.
IPR036890. HATPase_C_sf.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR036097. HisK_dim/P_sf.
IPR000014. PAS.
IPR035965. PAS-like_dom_sf.
IPR013767. PAS_fold.
IPR031967. PhoR_single_Cache-like_dom.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00989. PAS. 1 hit.
PF16736. sCache_like. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiView protein in PROSITE
PS50109. HIS_KIN. 1 hit.

Sequencei

Sequence statusi: Complete.

P23545-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKYRVRLFS VFVVCMILVF CVLGLFLQQL FETSDQRKAE EHIEKEAKYL
60 70 80 90 100
ASLLDAGNLN NQANEKIIKD AGGALDVSAS VIDTDGKVLY GSNGRSADSQ
110 120 130 140 150
KVQALVSGHE GILSTTDNKL YYGLSLRSEG EKTGYVLLSA SEKSDGLKGE
160 170 180 190 200
LWGMLTASLC TAFIVIVYFY SSMTSRYKRS IESATNVATE LSKGNYDART
210 220 230 240 250
YGGYIRRSDK LGHAMNSLAI DLMEMTRTQE MQRDRLLTVI ENIGSGLIMI
260 270 280 290 300
DGRGFINLVN RSYAKQFHIN PNHMLRRLYH DAFEHEEVIQ LVEDIFMTET
310 320 330 340 350
KKCKLLRLPI KIERRYFEVD GVPIMGPDDE WKGIVLVFHD MTETKKLEQM
360 370 380 390 400
RKDFVANVSH ELKTPITSIK GFTETLLDGA MEDKEALSEF LSIILKESER
410 420 430 440 450
LQSLVQDLLD LSKIEQQNFT LSIETFEPAK MLGEIETLLK HKADEKGISL
460 470 480 490 500
HLNVPKDPQY VSGDPYRLKQ VFLNLVNNAL TYTPEGGSVA INVKPREKDI
510 520 530 540 550
QIEVADSGIG IQKEEIPRIF ERFYRVDKDR SRNSGGTGLG LAIVKHLIEA
560 570
HEGKIDVTSE LGRGTVFTVT LKRAAEKSA
Length:579
Mass (Da):65,122
Last modified:November 1, 1991 - v1
Checksum:i0C4EE530A79A477B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M23549 Genomic DNA. Translation: AAA22663.1.
AF008220 Genomic DNA. Translation: AAC00349.1.
AL009126 Genomic DNA. Translation: CAB14870.1.
PIRiA27650.
RefSeqiNP_390788.1. NC_000964.3.
WP_004398493.1. NZ_JNCM01000036.1.

Genome annotation databases

EnsemblBacteriaiCAB14870; CAB14870; BSU29100.
GeneIDi937387.
KEGGibsu:BSU29100.
PATRICifig|224308.179.peg.3159.

Similar proteinsi

Entry informationi

Entry nameiPHOR_BACSU
AccessioniPrimary (citable) accession number: P23545
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 25, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references