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Protein

Aspartate aminotransferase, cytoplasmic

Gene

AAT2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a key role in amino acid metabolism.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.3 Publications

Cofactori

pyridoxal 5'-phosphate2 Publications

Kineticsi

  1. KM=0.11 mM for L-aspartate1 Publication
  2. KM=20 mM for L-glutamate1 Publication
  3. KM=0.006 mM for oxaloacetate1 Publication
  4. KM=0.16 mM for 2-oxoglutarate1 Publication

    pH dependencei

    Optimum pH is 8-9.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei38Aspartate; via amide nitrogen1 Publication1
    Binding sitei135Aspartate1 Publication1
    Binding sitei188Aspartate1 Publication1
    Binding sitei387Aspartate1 Publication1

    GO - Molecular functioni

    GO - Biological processi

    • 2-oxoglutarate metabolic process Source: UniProtKB
    • asparagine biosynthetic process from oxaloacetate Source: SGD
    • aspartate biosynthetic process Source: SGD
    • aspartate metabolic process Source: UniProtKB
    • glutamate metabolic process Source: UniProtKB
    Complete GO annotation...

    Keywords - Molecular functioni

    Aminotransferase, Transferase

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR027C-MONOMER.
    YEAST:YLR027C-MONOMER.
    ReactomeiR-SCE-70263. Gluconeogenesis.
    R-SCE-70614. Amino acid synthesis and interconversion (transamination).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartate aminotransferase, cytoplasmic (EC:2.6.1.1)
    Alternative name(s):
    Transaminase A
    Gene namesi
    Name:AAT2
    Synonyms:ASP5
    Ordered Locus Names:YLR027C
    ORF Names:L1746
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    Proteomesi
    • UP000002311 Componenti: Chromosome XII

    Organism-specific databases

    EuPathDBiFungiDB:YLR027C.
    SGDiS000004017. AAT2.

    Subcellular locationi

    GO - Cellular componenti

    • cytosol Source: SGD
    • peroxisome Source: SGD
    Complete GO annotation...

    Keywords - Cellular componenti

    Cytoplasm, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Initiator methionineiRemovedCombined sources2 Publications
    ChainiPRO_00001238752 – 418Aspartate aminotransferase, cytoplasmicAdd BLAST417

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei2N-acetylserineCombined sources1 Publication1
    Modified residuei255N6-(pyridoxal phosphate)lysine1 Publication1
    Modified residuei389PhosphoserineCombined sources1

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP23542.
    PRIDEiP23542.

    PTM databases

    iPTMnetiP23542.

    Interactioni

    Subunit structurei

    Homodimer.2 Publications

    GO - Molecular functioni

    Protein-protein interaction databases

    BioGridi31301. 178 interactors.
    DIPiDIP-2897N.
    IntActiP23542. 13 interactors.
    MINTiMINT-701237.

    Structurei

    Secondary structure

    1418
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Turni3 – 8Combined sources6
    Helixi18 – 25Combined sources8
    Helixi51 – 61Combined sources11
    Helixi77 – 88Combined sources12
    Helixi93 – 96Combined sources4
    Beta strandi100 – 106Combined sources7
    Helixi107 – 122Combined sources16
    Beta strandi128 – 133Combined sources6
    Helixi138 – 143Combined sources6
    Turni144 – 146Combined sources3
    Beta strandi149 – 153Combined sources5
    Turni157 – 160Combined sources4
    Helixi164 – 173Combined sources10
    Beta strandi179 – 183Combined sources5
    Turni188 – 190Combined sources3
    Helixi196 – 208Combined sources13
    Beta strandi212 – 218Combined sources7
    Turni220 – 222Combined sources3
    Beta strandi223 – 225Combined sources3
    Helixi227 – 230Combined sources4
    Helixi232 – 240Combined sources9
    Turni241 – 244Combined sources4
    Beta strandi247 – 252Combined sources6
    Turni254 – 256Combined sources3
    Helixi260 – 262Combined sources3
    Beta strandi264 – 270Combined sources7
    Helixi277 – 293Combined sources17
    Turni294 – 296Combined sources3
    Helixi301 – 312Combined sources12
    Helixi314 – 345Combined sources32
    Helixi353 – 356Combined sources4
    Beta strandi359 – 363Combined sources5
    Helixi368 – 378Combined sources11
    Beta strandi386 – 389Combined sources4
    Helixi390 – 392Combined sources3
    Turni395 – 397Combined sources3
    Helixi398 – 411Combined sources14

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1YAAX-ray2.05A/B/C/D2-412[»]
    ProteinModelPortaliP23542.
    SMRiP23542.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23542.

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    GeneTreeiENSGT00390000014081.
    HOGENOMiHOG000185898.
    InParanoidiP23542.
    KOiK14454.
    OMAiDPLFGLK.
    OrthoDBiEOG092C2B7X.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR000796. Asp_trans.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11879. PTHR11879. 1 hit.
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00799. TRANSAMINASE.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23542-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSATLFNNIE LLPPDALFGI KQRYGQDQRA TKVDLGIGAY RDDNGKPWVL
    60 70 80 90 100
    PSVKAAEKLI HNDSSYNHEY LGITGLPSLT SNAAKIIFGT QSDAFQEDRV
    110 120 130 140 150
    ISVQSLSGTG ALHISAKFFS KFFPDKLVYL SKPTWANHMA IFENQGLKTA
    160 170 180 190 200
    TYPYWANETK SLDLNGFLNA IQKAPEGSIF VLHSCAHNPT GLDPTSEQWV
    210 220 230 240 250
    QIVDAIASKN HIALFDTAYQ GFATGDLDKD AYAVRLGVEK LSTVSPVFVC
    260 270 280 290 300
    QSFAKNAGMY GERVGCFHLA LTKQAQNKTI KPAVTSQLAK IIRSEVSNPP
    310 320 330 340 350
    AYGAKIVAKL LETPELTEQW HKDMVTMSSR ITKMRHALRD HLVKLGTPGN
    360 370 380 390 400
    WDHIVNQCGM FSFTGLTPQM VKRLEETHAV YLVASGRASI AGLNQGNVEY
    410
    VAKAIDEVVR FYTIEAKL
    Length:418
    Mass (Da):46,058
    Last modified:January 23, 2007 - v3
    Checksum:iD25F40F6C6DD2B33
    GO

    Sequence cautioni

    The sequence CAA97550 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti95F → L AA sequence (PubMed:2199266).Curated1
    Sequence conflicti95F → L AA sequence (PubMed:1859361).Curated1
    Sequence conflicti413 – 414TI → AT AA sequence (PubMed:2199266).Curated2
    Sequence conflicti413 – 414TI → AT AA sequence (PubMed:1859361).Curated2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z73199 Genomic DNA. Translation: CAA97550.1. Different initiation.
    BK006945 Genomic DNA. Translation: DAA09345.1.
    PIRiS64854.
    RefSeqiNP_013127.2. NM_001181914.1.

    Genome annotation databases

    EnsemblFungiiYLR027C; YLR027C; YLR027C.
    GeneIDi850714.
    KEGGisce:YLR027C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Z73199 Genomic DNA. Translation: CAA97550.1. Different initiation.
    BK006945 Genomic DNA. Translation: DAA09345.1.
    PIRiS64854.
    RefSeqiNP_013127.2. NM_001181914.1.

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1YAAX-ray2.05A/B/C/D2-412[»]
    ProteinModelPortaliP23542.
    SMRiP23542.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi31301. 178 interactors.
    DIPiDIP-2897N.
    IntActiP23542. 13 interactors.
    MINTiMINT-701237.

    PTM databases

    iPTMnetiP23542.

    Proteomic databases

    MaxQBiP23542.
    PRIDEiP23542.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblFungiiYLR027C; YLR027C; YLR027C.
    GeneIDi850714.
    KEGGisce:YLR027C.

    Organism-specific databases

    EuPathDBiFungiDB:YLR027C.
    SGDiS000004017. AAT2.

    Phylogenomic databases

    GeneTreeiENSGT00390000014081.
    HOGENOMiHOG000185898.
    InParanoidiP23542.
    KOiK14454.
    OMAiDPLFGLK.
    OrthoDBiEOG092C2B7X.

    Enzyme and pathway databases

    BioCyciMetaCyc:YLR027C-MONOMER.
    YEAST:YLR027C-MONOMER.
    ReactomeiR-SCE-70263. Gluconeogenesis.
    R-SCE-70614. Amino acid synthesis and interconversion (transamination).

    Miscellaneous databases

    EvolutionaryTraceiP23542.
    PROiP23542.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    InterProiIPR004839. Aminotransferase_I/II.
    IPR000796. Asp_trans.
    IPR004838. NHTrfase_class1_PyrdxlP-BS.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    [Graphical view]
    PANTHERiPTHR11879. PTHR11879. 1 hit.
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    PRINTSiPR00799. TRANSAMINASE.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiAATC_YEAST
    AccessioniPrimary (citable) accession number: P23542
    Secondary accession number(s): D6VY29
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: January 23, 2007
    Last modified: November 2, 2016
    This is version 166 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
    Present with 7700 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XII
      Yeast (Saccharomyces cerevisiae) chromosome XII: entries and gene names

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.