P23540 (RNMC_MOMCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonuclease MC Short name=RNase MC EC=3.1.27.1
Organism	Momordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifier	3673 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Cucurbitales › Cucurbitaceae › Momordiceae › Momordica

Protein attributes

Sequence length	191 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.
Miscellaneous	Has a remarkably high specificity toward CPU, APU, and UPU.
Sequence similarities	Belongs to the RNase T2 family.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro ribonuclease T2 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 191

191

Ribonuclease MC

PRO_0000206508

Sites

Active site

34

1

By similarity

Active site

85

1

By similarity

Active site

89

1

By similarity

Amino acid modifications

Disulfide bond

48 ↔ 92

By similarity

Disulfide bond

152 ↔ 185

By similarity

Secondary structure

1

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191

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Helix

11 – 16

6

Helix

24 – 27

4

Beta strand

32 – 40

9

Helix

56 – 62

7

Helix

63 – 69

7

Beta strand

73 – 76

4

Helix

78 – 88

11

Helix

90 – 93

4

Turn

94 – 96

3

Helix

99 – 111

13

Helix

115 – 119

5

Helix

120 – 122

3

Beta strand

127 – 132

6

Helix

133 – 144

12

Beta strand

149 – 154

6

Turn

156 – 158

3

Beta strand

161 – 171

11

Beta strand

174 – 178

5

Beta strand

187 – 191

5

Sequences

	Sequence		Length	Mass (Da)
	P23540 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: A67958CB471DC7D2	FASTA	191	21,260
	10 20 30 40 50 60 FDSFWFVQQW PPAVCSFQKS GSCPGSGLRT FTIHGLWPQG SGTSLTNCPQ GSPFDITKIS 70 80 90 100 110 120 HLQSQLNTLW PNVLRANNQQ FWSHEWTKHG TCSESTFNQA AYFKLAVDMR NNYDIIGALR 130 140 150 160 170 180 PHAAGPNGRT KSRQAIKGFL KAKFGKFPGL RCRTDPQTKV SYLVQVVACF AQDGSTLIDC 190 TRDTCGANFI F

References

[1]	"The complete amino acid sequence of ribonuclease from the seeds of bitter gourd (Momordica charantia)." Ide H., Kimura M., Arai M., Funatsu G. FEBS Lett. 284:161-164(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Seed.
[2]	Erratum Ide H., Kimura M., Arai M., Funatsu G. FEBS Lett. 289:126-126(1991) [PubMed] [Europe PMC] [Abstract]
+	Additional computationally mapped references.

Cross-references

Sequence databases

PIR

S17505.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BK7	X-ray	1.75	A	1-191	[»]
1J1F	X-ray	1.60	A	1-191	[»]
1J1G	X-ray	1.60	A	2-191	[»]
1UCA	X-ray	1.48	A	1-191	[»]
1UCC	X-ray	1.77	A	1-191	[»]
1UCD	X-ray	1.30	A	1-191	[»]
1UCG	X-ray	1.65	A/B	2-191	[»]
1V9H	X-ray	2.00	A	1-191	[»]

ProteinModelPortal

P23540.

SMR

P23540. Positions 1-191.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BRENDA

3.1.27.1. 3398.

Family and domain databases

Gene3D

3.90.730.10. 1 hit.

InterPro

IPR001568. RNase_T2-like.
IPR018188. RNase_T2_AS.
[Graphical view]

PANTHER

PTHR11240. PTHR11240. 1 hit.

Pfam

PF00445. Ribonuclease_T2. 1 hit.
[Graphical view]

SUPFAM

SSF55895. RNase_T2. 1 hit.

PROSITE

PS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23540.

Entry information

Entry name

RNMC_MOMCH

Accession

Primary (citable) accession number: P23540

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program