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Protein

Ribonuclease MC

Gene
N/A
Organism
Momordica charantia (Bitter gourd) (Balsam pear)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides with 2',3'-cyclic phosphate intermediates.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei34 – 341By similarity
Active sitei85 – 851By similarity
Active sitei89 – 891By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Enzyme and pathway databases

BRENDAi3.1.27.1. 3398.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease MC (EC:3.1.27.1)
Short name:
RNase MC
OrganismiMomordica charantia (Bitter gourd) (Balsam pear)
Taxonomic identifieri3673 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeMomordiceaeMomordica

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 191191Ribonuclease MCPRO_0000206508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi48 ↔ 92By similarity
Disulfide bondi152 ↔ 185By similarity

Keywords - PTMi

Disulfide bond

Structurei

Secondary structure

1
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 97Combined sources
Helixi11 – 166Combined sources
Helixi24 – 274Combined sources
Beta strandi32 – 409Combined sources
Helixi56 – 627Combined sources
Helixi63 – 697Combined sources
Beta strandi73 – 764Combined sources
Helixi78 – 8811Combined sources
Helixi90 – 934Combined sources
Turni94 – 963Combined sources
Helixi99 – 11113Combined sources
Helixi115 – 1195Combined sources
Helixi120 – 1223Combined sources
Beta strandi127 – 1326Combined sources
Helixi133 – 14412Combined sources
Beta strandi149 – 1546Combined sources
Turni156 – 1583Combined sources
Beta strandi161 – 17111Combined sources
Beta strandi174 – 1785Combined sources
Beta strandi187 – 1915Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BK7X-ray1.75A1-191[»]
1J1FX-ray1.60A1-191[»]
1J1GX-ray1.60A2-191[»]
1UCAX-ray1.48A1-191[»]
1UCCX-ray1.77A1-191[»]
1UCDX-ray1.30A1-191[»]
1UCGX-ray1.65A/B2-191[»]
1V9HX-ray2.00A1-191[»]
ProteinModelPortaliP23540.
SMRiP23540. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23540.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNase T2 family.Curated

Family and domain databases

CDDicd01061. RNase_T2_euk. 1 hit.
Gene3Di3.90.730.10. 1 hit.
InterProiIPR033697. Ribonuclease_T2_eukaryotic.
IPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23540-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
FDSFWFVQQW PPAVCSFQKS GSCPGSGLRT FTIHGLWPQG SGTSLTNCPQ
60 70 80 90 100
GSPFDITKIS HLQSQLNTLW PNVLRANNQQ FWSHEWTKHG TCSESTFNQA
110 120 130 140 150
AYFKLAVDMR NNYDIIGALR PHAAGPNGRT KSRQAIKGFL KAKFGKFPGL
160 170 180 190
RCRTDPQTKV SYLVQVVACF AQDGSTLIDC TRDTCGANFI F
Length:191
Mass (Da):21,260
Last modified:November 1, 1991 - v1
Checksum:iA67958CB471DC7D2
GO

Sequence databases

PIRiS17505.

Cross-referencesi

Sequence databases

PIRiS17505.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1BK7X-ray1.75A1-191[»]
1J1FX-ray1.60A1-191[»]
1J1GX-ray1.60A2-191[»]
1UCAX-ray1.48A1-191[»]
1UCCX-ray1.77A1-191[»]
1UCDX-ray1.30A1-191[»]
1UCGX-ray1.65A/B2-191[»]
1V9HX-ray2.00A1-191[»]
ProteinModelPortaliP23540.
SMRiP23540. Positions 1-191.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.27.1. 3398.

Miscellaneous databases

EvolutionaryTraceiP23540.

Family and domain databases

CDDicd01061. RNase_T2_euk. 1 hit.
Gene3Di3.90.730.10. 1 hit.
InterProiIPR033697. Ribonuclease_T2_eukaryotic.
IPR001568. RNase_T2-like.
IPR018188. RNase_T2_His_AS_1.
IPR033130. RNase_T2_His_AS_2.
[Graphical view]
PANTHERiPTHR11240. PTHR11240. 1 hit.
PfamiPF00445. Ribonuclease_T2. 1 hit.
[Graphical view]
SUPFAMiSSF55895. SSF55895. 1 hit.
PROSITEiPS00530. RNASE_T2_1. 1 hit.
PS00531. RNASE_T2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNMC_MOMCH
AccessioniPrimary (citable) accession number: P23540
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Has a remarkably high specificity toward CPU, APU, and UPU.

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.