P23535 (E13B_PHAVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glucan endo-1,3-beta-glucosidase, basic isoform EC=3.2.1.39 Alternative name(s): (1->3)-beta-glucan endohydrolase Short name=(1->3)-beta-glucanase Beta-1,3-endoglucanase
Organism	Phaseolus vulgaris (Kidney bean) (French bean)
Taxonomic identifier	3885 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Phaseolus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole By similarity.
Induction	By fungal elicitor.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Molecular function	Glycosidase Hydrolase
PTM	Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 316	316	Glucan endo-1,3-beta-glucosidase, basic isoform		PRO_0000011857
	Propeptide	317 – 348	32	Removed in mature form By similarity		PRO_0000011858
Sites
	Active site	240	1	Nucleophile By similarity
	Active site	297	1	Proton donor By similarity
Amino acid modifications
	Modified residue	1	1	Pyrrolidone carboxylic acid Probable

Sequences

	Sequence		Length	Mass (Da)
	P23535 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 83177308F77429D6	FASTA	348	38,869
	10 20 30 40 50 60 QIGVCYGMMG NNLPSANEVI NLYRSNNIRR MRLYDPNQAA LQALRNSGIE LILGVPNSDL 70 80 90 100 110 120 QGLATNADTA RQWVQRNVLN FWPSVKIKYI AVGNEVSPVG GSSWYAQYVL PAVQNVYQAV 130 140 150 160 170 180 RAQGLHDQIK VSTAIDMTLI GNSYPPSQGS FRGDVRSYLD PIIGYLLYAS APLHVNVYPY 190 200 210 220 230 240 FSYSGNPRDI SLPYALFTSP NVVVRDGQYG YQNLFDAMLD SVHAAIDNTR IGYVEVVVSE 250 260 270 280 290 300 SGWPSDGGFG ATYDNARVYL DNLVRRAGRG SPRRPSKPTE TYIFAMFDEN QKSPEIEKHF 310 320 330 340 GLFKPSKEKK YPFGFGAQRM QRLLLMSSMQ HIPLRVTCKL EPSSQSLL

References

[1]	"cDNA cloning and characterization of a putative 1,3-beta-D-glucanase transcript induced by fungal elicitor in bean cell suspension cultures." Edington B.V., Lamb C.J., Dixon R.A. Plant Mol. Biol. 16:81-94(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53129 mRNA. Translation: CAA37289.1.
PIR	S13323.
3D structure databases
ProteinModelPortal	P23535.
SMR	P23535. Positions 2-315.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_PHAVU

Accession

Primary (citable) accession number: P23535

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program