Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

PTS system lactose-specific EIIA component

Gene

lacF

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per trimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Tele-phosphohistidine intermediate1 Publication1
Metal bindingi81Magnesium; shared with all trimeric partners1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIIA component1 Publication (EC:2.7.1.207By similarity)
Alternative name(s):
EIIA-Lac1 Publication
EIII-Lac1 Publication
Lactose-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:lacF1 Publication
Encoded oniPlasmid pLP7120 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18G → E: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865971 – 105PTS system lactose-specific EIIA componentAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78Phosphohistidine; by HPrPROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.1 Publication

Interactioni

Subunit structurei

Homotrimer.3 Publications

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 32Combined sources30
Helixi36 – 65Combined sources30
Helixi74 – 103Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E2AX-ray2.30A/B/C1-105[»]
2E2AX-ray2.10A/B/C1-105[»]
ProteinModelPortaliP23532.
SMRiP23532.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23532.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 102PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST99

Domaini

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation

Sequence similaritiesi

Contains 1 PTS EIIA type-3 domain.PROSITE-ProRule annotation

Family and domain databases

CDDicd00215. PTS_IIA_lac. 1 hit.
Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac/cel.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI
60 70 80 90 100
AEAHSSQTGM LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY

KRGAK
Length:105
Mass (Da):11,448
Last modified:November 1, 1991 - v1
Checksum:iD2ACDEF3433A8C6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60447 Genomic DNA. Translation: AAA25181.1.
M65190 Genomic DNA. No translation available.
PIRiA23696.
RefSeqiWP_014011534.1. NZ_LKLY01000068.1.
YP_004761516.1. NC_015862.1.

Genome annotation databases

GeneIDi11013277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60447 Genomic DNA. Translation: AAA25181.1.
M65190 Genomic DNA. No translation available.
PIRiA23696.
RefSeqiWP_014011534.1. NZ_LKLY01000068.1.
YP_004761516.1. NC_015862.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E2AX-ray2.30A/B/C1-105[»]
2E2AX-ray2.10A/B/C1-105[»]
ProteinModelPortaliP23532.
SMRiP23532.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi11013277.

Miscellaneous databases

EvolutionaryTraceiP23532.

Family and domain databases

CDDicd00215. PTS_IIA_lac. 1 hit.
Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac/cel.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTLA_LACLL
AccessioniPrimary (citable) accession number: P23532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.