P23532 (PTLA_LACLL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lactose-specific phosphotransferase enzyme IIA component
EC=2.7.1.-

Alternative name(s):
EIIA-Lac
EIII-Lac
PTS system lactose-specific EIIA component

Gene names

Name:

lacF

Encoded on

Plasmid pLP712

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › Lactococcus lactis

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Cofactor	Binds 1 magnesium ion per trimer.
Subunit structure	Homotrimer. Ref.1
Subcellular location	Cytoplasm.
Induction	By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced. Ref.1
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Miscellaneous	This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.
Sequence similarities	Contains 1 PTS EIIA type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	3D-structure Direct protein sequencing Plasmid
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: InterPro
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 105

105

Lactose-specific phosphotransferase enzyme IIA component

PRO_0000186597

Regions

Domain

4 – 102

99

PTS EIIA type-3

Sites

Active site

78

1

Tele-phosphohistidine intermediate; by HPr

Metal binding

81

1

Magnesium; shared with all trimeric partners

Experimental info

Mutagenesis

18

1

G → E: No activity. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

105

Helix Strand Turn

Details...

Helix

3 – 32

30

Helix

36 – 65

30

Helix

74 – 103

30

Sequences

	Sequence		Length	Mass (Da)
	P23532 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: D2ACDEF3433A8C6B	FASTA	105	11,448
	10 20 30 40 50 60 MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI AEAHSSQTGM 70 80 90 100 LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY KRGAK

References

[1]	"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis." de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W. J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, MUTAGENESIS OF GLY-18, SUBUNIT, OPERON STRUCTURE, INDUCTION. Strain: MG1820.
[2]	"Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis." van Rooijen R.J., van Schalkwijk S., de Vos W.M. J. Biol. Chem. 266:7176-7181(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11. Strain: MG1820.
[3]	"The structure of enzyme IIAlactose from Lactococcus lactis reveals a new fold and points to possible interactions of a multicomponent system." Sliz P., Engelmann R., Hengstenberg W., Pai E.F. Structure 5:775-788(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M60447 Genomic DNA. Translation: AAA25181.1.
M65190 Genomic DNA. No translation available.

PIR

A23696.

RefSeq

YP_004761516.1. NC_015862.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E2A	X-ray	2.30	A/B/C	1-105	[»]
2E2A	X-ray	2.10	A/B/C	1-105	[»]

ProteinModelPortal

P23532.

SMR

P23532. Positions 1-102.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

11013277.

Family and domain databases

Gene3D

1.20.58.80. 1 hit.

InterPro

IPR003188. PTS_IIA_lac.
[Graphical view]

Pfam

PF02255. PTS_IIA. 1 hit.
[Graphical view]

PIRSF

PIRSF000699. PTS_IILac_III. 1 hit.

SUPFAM

SSF46973. Ptrans_IIA. 1 hit.

TIGRFAMs

TIGR00823. EIIA-LAC. 1 hit.

PROSITE

PS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P23532.

Entry information

Entry name

PTLA_LACLL

Accession

Primary (citable) accession number: P23532

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program