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P23532 (PTLA_LACLL) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lactose-specific phosphotransferase enzyme IIA component

EC=2.7.1.-
Alternative name(s):
EIIA-Lac
EIII-Lac
PTS system lactose-specific EIIA component
Gene names
Name:lacF
Encoded onPlasmid pLP712
OrganismLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifier1360 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Protein attributes

Sequence length105 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.

Cofactor

Binds 1 magnesium ion per trimer.

Subunit structure

Homotrimer. Ref.1

Subcellular location

Cytoplasm.

Induction

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced. Ref.1

Domain

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

Sequence similarities

Contains 1 PTS EIIA type-3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 105105Lactose-specific phosphotransferase enzyme IIA component
PRO_0000186597

Regions

Domain4 – 10299PTS EIIA type-3

Sites

Active site781Tele-phosphohistidine intermediate; by HPr
Metal binding811Magnesium; shared with all trimeric partners

Experimental info

Mutagenesis181G → E: No activity. Ref.1

Secondary structure

....... 105
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P23532 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: D2ACDEF3433A8C6B

FASTA10511,448
        10         20         30         40         50         60 
MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI AEAHSSQTGM 

        70         80         90        100 
LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY KRGAK 

« Hide

References

[1]"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, MUTAGENESIS OF GLY-18, SUBUNIT, OPERON STRUCTURE, INDUCTION.
Strain: MG1820.
[2]"Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis."
van Rooijen R.J., van Schalkwijk S., de Vos W.M.
J. Biol. Chem. 266:7176-7181(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
Strain: MG1820.
[3]"The structure of enzyme IIAlactose from Lactococcus lactis reveals a new fold and points to possible interactions of a multicomponent system."
Sliz P., Engelmann R., Hengstenberg W., Pai E.F.
Structure 5:775-788(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60447 Genomic DNA. Translation: AAA25181.1.
M65190 Genomic DNA. No translation available.
PIRA23696.
RefSeqYP_004761516.1. NC_015862.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1E2AX-ray2.30A/B/C1-105[»]
2E2AX-ray2.10A/B/C1-105[»]
ProteinModelPortalP23532.
SMRP23532. Positions 1-102.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID11013277.

Family and domain databases

Gene3D1.20.58.80. 1 hit.
InterProIPR003188. PTS_IIA_lac.
[Graphical view]
PfamPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMSSF46973. SSF46973. 1 hit.
TIGRFAMsTIGR00823. EIIA-LAC. 1 hit.
PROSITEPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP23532.

Entry information

Entry namePTLA_LACLL
AccessionPrimary (citable) accession number: P23532
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: April 16, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references