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Protein

PTS system lactose-specific EIIA component

Gene

lacF

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.1 Publication

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.1 Publication

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per trimer.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei78Tele-phosphohistidine intermediate1 Publication1
Metal bindingi81Magnesium; shared with all trimeric partners1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPhosphotransferase system, Sugar transport, Transport
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIIA component1 Publication
Alternative name(s):
EIIA-Lac1 Publication
EIII-Lac1 Publication
Lactose-specific phosphotransferase enzyme IIA component1 Publication
Gene namesi
Name:lacF1 Publication
Encoded oniPlasmid pLP7120 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi18G → E: No activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865971 – 105PTS system lactose-specific EIIA componentAdd BLAST105

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78Phosphohistidine; by HPrPROSITE-ProRule annotation1 Publication1

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP23532

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.1 Publication

Interactioni

Subunit structurei

Homotrimer.3 Publications

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 32Combined sources30
Helixi36 – 65Combined sources30
Helixi74 – 103Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E2AX-ray2.30A/B/C1-105[»]
2E2AX-ray2.10A/B/C1-105[»]
ProteinModelPortaliP23532
SMRiP23532
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23532

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 102PTS EIIA type-3PROSITE-ProRule annotationAdd BLAST99

Domaini

The PTS EIIA type-3 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the PTS EIIB type-3 domain.PROSITE-ProRule annotation

Family and domain databases

CDDicd00215 PTS_IIA_lac, 1 hit
InterProiView protein in InterPro
IPR003188 PTS_IIA_lac/cel
IPR036542 PTS_IIA_lac/cel_sf
PANTHERiPTHR34382 PTHR34382, 1 hit
PfamiView protein in Pfam
PF02255 PTS_IIA, 1 hit
PIRSFiPIRSF000699 PTS_IILac_III, 1 hit
SUPFAMiSSF46973 SSF46973, 1 hit
TIGRFAMsiTIGR00823 EIIA-LAC, 1 hit
PROSITEiView protein in PROSITE
PS51095 PTS_EIIA_TYPE_3, 1 hit

Sequencei

Sequence statusi: Complete.

P23532-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI
60 70 80 90 100
AEAHSSQTGM LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY

KRGAK
Length:105
Mass (Da):11,448
Last modified:November 1, 1991 - v1
Checksum:iD2ACDEF3433A8C6B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60447 Genomic DNA Translation: AAA25181.1
M65190 Genomic DNA No translation available.
PIRiA23696
RefSeqiWP_014011534.1, NZ_MUHS01000071.1
YP_004761516.1, NC_015862.1

Genome annotation databases

GeneIDi11013277

Similar proteinsi

Entry informationi

Entry nameiPTLA_LACLL
AccessioniPrimary (citable) accession number: P23532
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: March 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid
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Main funding by: National Institutes of Health