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P23532

- PTLA_LACLL

UniProt

P23532 - PTLA_LACLL

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Protein

Lactose-specific phosphotransferase enzyme IIA component

Gene

lacF

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Cofactori

Binds 1 magnesium ion per trimer.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Tele-phosphohistidine intermediate; by HPr
Metal bindingi81 – 811Magnesium; shared with all trimeric partners

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Lactose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
EIIA-Lac
EIII-Lac
PTS system lactose-specific EIIA component
Gene namesi
Name:lacF
Encoded oniPlasmid pLP7120 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi18 – 181G → E: No activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 105105Lactose-specific phosphotransferase enzyme IIA componentPRO_0000186597Add
BLAST

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.1 Publication

Interactioni

Subunit structurei

Homotrimer.1 Publication

Structurei

Secondary structure

1
105
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 3230
Helixi36 – 6530
Helixi74 – 10330

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E2AX-ray2.30A/B/C1-105[»]
2E2AX-ray2.10A/B/C1-105[»]
ProteinModelPortaliP23532.
SMRiP23532. Positions 1-102.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23532.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 10299PTS EIIA type-3PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-3 domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.20.58.80. 1 hit.
InterProiIPR003188. PTS_IIA_lac.
[Graphical view]
PfamiPF02255. PTS_IIA. 1 hit.
[Graphical view]
PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMiSSF46973. SSF46973. 1 hit.
TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23532-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI
60 70 80 90 100
AEAHSSQTGM LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY

KRGAK
Length:105
Mass (Da):11,448
Last modified:November 1, 1991 - v1
Checksum:iD2ACDEF3433A8C6B
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60447 Genomic DNA. Translation: AAA25181.1.
M65190 Genomic DNA. No translation available.
PIRiA23696.
RefSeqiWP_014011534.1. NC_015862.1.
YP_004761516.1. NC_015862.1.

Genome annotation databases

GeneIDi11013277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60447 Genomic DNA. Translation: AAA25181.1 .
M65190 Genomic DNA. No translation available.
PIRi A23696.
RefSeqi WP_014011534.1. NC_015862.1.
YP_004761516.1. NC_015862.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E2A X-ray 2.30 A/B/C 1-105 [» ]
2E2A X-ray 2.10 A/B/C 1-105 [» ]
ProteinModelPortali P23532.
SMRi P23532. Positions 1-102.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 11013277.

Miscellaneous databases

EvolutionaryTracei P23532.

Family and domain databases

Gene3Di 1.20.58.80. 1 hit.
InterProi IPR003188. PTS_IIA_lac.
[Graphical view ]
Pfami PF02255. PTS_IIA. 1 hit.
[Graphical view ]
PIRSFi PIRSF000699. PTS_IILac_III. 1 hit.
SUPFAMi SSF46973. SSF46973. 1 hit.
TIGRFAMsi TIGR00823. EIIA-LAC. 1 hit.
PROSITEi PS51095. PTS_EIIA_TYPE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
    de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
    J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, MUTAGENESIS OF GLY-18, SUBUNIT, OPERON STRUCTURE, INDUCTION.
    Strain: MG1820.
  2. "Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis."
    van Rooijen R.J., van Schalkwijk S., de Vos W.M.
    J. Biol. Chem. 266:7176-7181(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
    Strain: MG1820.
  3. "The structure of enzyme IIAlactose from Lactococcus lactis reveals a new fold and points to possible interactions of a multicomponent system."
    Sliz P., Engelmann R., Hengstenberg W., Pai E.F.
    Structure 5:775-788(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

Entry informationi

Entry nameiPTLA_LACLL
AccessioniPrimary (citable) accession number: P23532
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

Keywords - Technical termi

3D-structure, Direct protein sequencing, Plasmid

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3