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P23532

- PTLA_LACLL

UniProt

P23532 - PTLA_LACLL

Protein

Lactose-specific phosphotransferase enzyme IIA component

Gene

lacF

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system is involved in lactose transport.

    Catalytic activityi

    Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion per trimer.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei78 – 781Tele-phosphohistidine intermediate; by HPr
    Metal bindingi81 – 811Magnesium; shared with all trimeric partners

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Phosphotransferase system, Sugar transport, Transport

    Keywords - Ligandi

    Magnesium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lactose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
    Alternative name(s):
    EIIA-Lac
    EIII-Lac
    PTS system lactose-specific EIIA component
    Gene namesi
    Name:lacF
    Encoded oniPlasmid pLP7120 Publication
    OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
    Taxonomic identifieri1360 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi18 – 181G → E: No activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 105105Lactose-specific phosphotransferase enzyme IIA componentPRO_0000186597Add
    BLAST

    Expressioni

    Inductioni

    By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.1 Publication

    Interactioni

    Subunit structurei

    Homotrimer.1 Publication

    Structurei

    Secondary structure

    1
    105
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi3 – 3230
    Helixi36 – 6530
    Helixi74 – 10330

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1E2AX-ray2.30A/B/C1-105[»]
    2E2AX-ray2.10A/B/C1-105[»]
    ProteinModelPortaliP23532.
    SMRiP23532. Positions 1-102.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP23532.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 10299PTS EIIA type-3PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

    Sequence similaritiesi

    Contains 1 PTS EIIA type-3 domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.20.58.80. 1 hit.
    InterProiIPR003188. PTS_IIA_lac.
    [Graphical view]
    PfamiPF02255. PTS_IIA. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000699. PTS_IILac_III. 1 hit.
    SUPFAMiSSF46973. SSF46973. 1 hit.
    TIGRFAMsiTIGR00823. EIIA-LAC. 1 hit.
    PROSITEiPS51095. PTS_EIIA_TYPE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23532-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNREEMTLLG FEIVAYAGDA RSKLLEALKA AENGDFAKAD SLVVEAGSCI    50
    AEAHSSQTGM LAREASGEEL PYSVTMMHGQ DHLMTTILLK DVIHHLIELY 100
    KRGAK 105
    Length:105
    Mass (Da):11,448
    Last modified:November 1, 1991 - v1
    Checksum:iD2ACDEF3433A8C6B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60447 Genomic DNA. Translation: AAA25181.1.
    M65190 Genomic DNA. No translation available.
    PIRiA23696.
    RefSeqiWP_014011534.1. NC_015862.1.
    YP_004761516.1. NC_015862.1.

    Genome annotation databases

    GeneIDi11013277.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60447 Genomic DNA. Translation: AAA25181.1 .
    M65190 Genomic DNA. No translation available.
    PIRi A23696.
    RefSeqi WP_014011534.1. NC_015862.1.
    YP_004761516.1. NC_015862.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1E2A X-ray 2.30 A/B/C 1-105 [» ]
    2E2A X-ray 2.10 A/B/C 1-105 [» ]
    ProteinModelPortali P23532.
    SMRi P23532. Positions 1-102.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 11013277.

    Miscellaneous databases

    EvolutionaryTracei P23532.

    Family and domain databases

    Gene3Di 1.20.58.80. 1 hit.
    InterProi IPR003188. PTS_IIA_lac.
    [Graphical view ]
    Pfami PF02255. PTS_IIA. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000699. PTS_IILac_III. 1 hit.
    SUPFAMi SSF46973. SSF46973. 1 hit.
    TIGRFAMsi TIGR00823. EIIA-LAC. 1 hit.
    PROSITEi PS51095. PTS_EIIA_TYPE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
      de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
      J. Biol. Chem. 265:22554-22560(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-23, MUTAGENESIS OF GLY-18, SUBUNIT, OPERON STRUCTURE, INDUCTION.
      Strain: MG1820.
    2. "Molecular cloning, characterization, and nucleotide sequence of the tagatose 6-phosphate pathway gene cluster of the lactose operon of Lactococcus lactis."
      van Rooijen R.J., van Schalkwijk S., de Vos W.M.
      J. Biol. Chem. 266:7176-7181(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-11.
      Strain: MG1820.
    3. "The structure of enzyme IIAlactose from Lactococcus lactis reveals a new fold and points to possible interactions of a multicomponent system."
      Sliz P., Engelmann R., Hengstenberg W., Pai E.F.
      Structure 5:775-788(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS).

    Entry informationi

    Entry nameiPTLA_LACLL
    AccessioniPrimary (citable) accession number: P23532
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

    Keywords - Technical termi

    3D-structure, Direct protein sequencing, Plasmid

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3