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Protein

PTS system lactose-specific EIICB component

Gene

lacE

Organism
Lactococcus lactis subsp. lactis (Streptococcus lactis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.1 Publication

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.1 Publication

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei473Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIICB component1 Publication
Alternative name(s):
EIICB-Lac1 Publication
Short name:
EII-Lac1 Publication
Including the following 2 domains:
PTS system lactose-specific EIIC component1 Publication
Alternative name(s):
Lactose permease IIC component1 Publication
PTS system lactose-specific EIIB component1 Publication (EC:2.7.1.207By similarity)
Alternative name(s):
Lactose-specific phosphotransferase enzyme IIB component1 Publication
Gene namesi
Name:lacE1 Publication
Encoded oniPlasmid pLP7120 Publication
OrganismiLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifieri1360 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeLactococcus

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotation; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei62 – 82HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei103 – 123HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei128 – 148HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei183 – 203HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei222 – 242HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei339 – 359HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei389 – 409HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865841 – 568PTS system lactose-specific EIICB componentAdd BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei473Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced.1 Publication

Structurei

3D structure databases

ProteinModelPortaliP23531.
SMRiP23531.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 409PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST403
Domaini466 – 568PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST103

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiView protein in InterPro
IPR036095. PTS_EIIB-like_sf.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004801. PTS_IIC_lac_spec.
PfamiView protein in Pfam
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00394. lac_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
TIGR00853. pts-lac. 1 hit.
PROSITEiView protein in PROSITE
PS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P23531-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHKLIELIEK GKPFFEKISR NIYLRAIRDG FIAGMPVILF SSIFILIAYV
60 70 80 90 100
PNAWGFHWSK DIETFLMTPY SYSMGILAFF VGGTTAKALT DSKNRDLPAT
110 120 130 140 150
NQINFLSTML ASMVGFLLMA AEPAKEGGFL TAFMGTKGLL TAFIAAFVTV
160 170 180 190 200
NVYKVCVKNN VTIRMPEDVP PNISQVFKDL IPFTVSVVLL YGLELLVKGT
210 220 230 240 250
LGVTVAESIG TLIAPLFSAA DGYLGITLIF GAYAFFWFVG IHGPSIVEPA
260 270 280 290 300
IAAITYANID VNLHLIQAGQ HADKVITSGT QMFIATMGGT GATLIVPFLF
310 320 330 340 350
MWICKSDRNR AIGRASVVPT FFGVNEPILF GAPIVLNPIF FVPFIFAPIV
360 370 380 390 400
NVWIFKFFVD TLNMNSFSAN LPWVTPGPLG IVLGTNFQVL SFILAGLLVV
410 420 430 440 450
VDTIIYYPFV KVYDEQILEE ERSGKTNDAL KEKVAANFNT AKADAVLGKA
460 470 480 490 500
DVAKEDVAAN NNITKETNVL VLCAGGGTSG LLANALNKAA AEYNVPVKAA
510 520 530 540 550
AGGYGAHREM LPEFDLVILA PQVASNFDDM KAETDKLGIK LVKTEGAQYI
560
KLTRDGQGAL AFVQQQFD
Length:568
Mass (Da):61,563
Last modified:November 1, 1991 - v1
Checksum:iBE5ECA7736762446
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60447 Genomic DNA. Translation: AAA25182.1.
PIRiB23696.

Similar proteinsi

Entry informationi

Entry nameiPTLCB_LACLL
AccessioniPrimary (citable) accession number: P23531
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 25, 2017
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Plasmid