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P23531 (PTLCB_LACLL) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system lactose-specific EIICB component
Alternative name(s):
EIICB-Lac
Short name=EII-Lac

Including the following 2 domains:

  1. Lactose permease IIC component
    Alternative name(s):
    PTS system lactose-specific EIIC component
  2. Lactose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system lactose-specific EIIB component
Gene names
Name:lacE
Encoded onPlasmid pLP712
OrganismLactococcus lactis subsp. lactis (Streptococcus lactis)
Taxonomic identifier1360 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length568 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Induction

By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced. Ref.1

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Miscellaneous

This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.

Sequence similarities

Contains 1 PTS EIIB type-3 domain.

Contains 1 PTS EIIC type-3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 568568PTS system lactose-specific EIICB component
PRO_0000186584

Regions

Transmembrane29 – 4921Helical; Potential
Transmembrane61 – 8121Helical; Potential
Transmembrane101 – 12121Helical; Potential
Transmembrane127 – 14721Helical; Potential
Transmembrane218 – 23821Helical; Potential
Transmembrane278 – 29821Helical; Potential
Transmembrane333 – 35321Helical; Potential
Transmembrane382 – 40221Helical; Potential
Domain7 – 409403PTS EIIC type-3
Domain466 – 568103PTS EIIB type-3

Sites

Active site4731Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P23531 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: BE5ECA7736762446

FASTA56861,563
        10         20         30         40         50         60 
MHKLIELIEK GKPFFEKISR NIYLRAIRDG FIAGMPVILF SSIFILIAYV PNAWGFHWSK 

        70         80         90        100        110        120 
DIETFLMTPY SYSMGILAFF VGGTTAKALT DSKNRDLPAT NQINFLSTML ASMVGFLLMA 

       130        140        150        160        170        180 
AEPAKEGGFL TAFMGTKGLL TAFIAAFVTV NVYKVCVKNN VTIRMPEDVP PNISQVFKDL 

       190        200        210        220        230        240 
IPFTVSVVLL YGLELLVKGT LGVTVAESIG TLIAPLFSAA DGYLGITLIF GAYAFFWFVG 

       250        260        270        280        290        300 
IHGPSIVEPA IAAITYANID VNLHLIQAGQ HADKVITSGT QMFIATMGGT GATLIVPFLF 

       310        320        330        340        350        360 
MWICKSDRNR AIGRASVVPT FFGVNEPILF GAPIVLNPIF FVPFIFAPIV NVWIFKFFVD 

       370        380        390        400        410        420 
TLNMNSFSAN LPWVTPGPLG IVLGTNFQVL SFILAGLLVV VDTIIYYPFV KVYDEQILEE 

       430        440        450        460        470        480 
ERSGKTNDAL KEKVAANFNT AKADAVLGKA DVAKEDVAAN NNITKETNVL VLCAGGGTSG 

       490        500        510        520        530        540 
LLANALNKAA AEYNVPVKAA AGGYGAHREM LPEFDLVILA PQVASNFDDM KAETDKLGIK 

       550        560 
LVKTEGAQYI KLTRDGQGAL AFVQQQFD

« Hide

References

[1]"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis."
de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W.
J. Biol. Chem. 265:22554-22560(1990) [PubMed: 2125052] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, INDUCTION.
Strain: MG1820.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M60447 Genomic DNA. Translation: AAA25182.1.
PIRB23696.

3D structure databases

ProteinModelPortalP23531.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR014350. PTrfase_system_EIIB_3_subgr.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_IIC_lac.
IPR004801. PTS_IIC_lac_spec.
[Graphical view]
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00394. Lac_pts_IIC. 1 hit.
TIGR00410. LacE. 1 hit.
TIGR00853. Pts-lac. 1 hit.
PROSITEPS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTLCB_LACLL
AccessionPrimary (citable) accession number: P23531
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 19, 2011
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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