Reviewed, UniProtKB/Swiss-Prot P23531 (PTLCB_LACLA)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:

lacE

Encoded on

Plasmid pLP712

Organism

Lactococcus lactis subsp. lactis (Streptococcus lactis)

Taxonomic identifier

1360 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Induction	By lactose. The operon consists of lacABCDFEGX. A second transcript of only lacF and lacE is also lactose-induced. Ref.1
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Miscellaneous	This gene was sequenced from pMG820, a laboratory-derived deletion of the naturally occurring plasmid pLP712.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Plasmid
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	PTS system lactose-specific EIICB component		PRO_0000186584
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	61 – 81	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	127 – 147	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	278 – 298	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	382 – 402	21	Potential
	Domain	7 – 409	403	PTS EIIC type-3
	Domain	466 – 568	103	PTS EIIB type-3
Sites
	Active site	473	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23531-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: BE5ECA7736762446	FASTA	568	61,563
	10 20 30 40 50 60 MHKLIELIEK GKPFFEKISR NIYLRAIRDG FIAGMPVILF SSIFILIAYV PNAWGFHWSK 70 80 90 100 110 120 DIETFLMTPY SYSMGILAFF VGGTTAKALT DSKNRDLPAT NQINFLSTML ASMVGFLLMA 130 140 150 160 170 180 AEPAKEGGFL TAFMGTKGLL TAFIAAFVTV NVYKVCVKNN VTIRMPEDVP PNISQVFKDL 190 200 210 220 230 240 IPFTVSVVLL YGLELLVKGT LGVTVAESIG TLIAPLFSAA DGYLGITLIF GAYAFFWFVG 250 260 270 280 290 300 IHGPSIVEPA IAAITYANID VNLHLIQAGQ HADKVITSGT QMFIATMGGT GATLIVPFLF 310 320 330 340 350 360 MWICKSDRNR AIGRASVVPT FFGVNEPILF GAPIVLNPIF FVPFIFAPIV NVWIFKFFVD 370 380 390 400 410 420 TLNMNSFSAN LPWVTPGPLG IVLGTNFQVL SFILAGLLVV VDTIIYYPFV KVYDEQILEE 430 440 450 460 470 480 ERSGKTNDAL KEKVAANFNT AKADAVLGKA DVAKEDVAAN NNITKETNVL VLCAGGGTSG 490 500 510 520 530 540 LLANALNKAA AEYNVPVKAA AGGYGAHREM LPEFDLVILA PQVASNFDDM KAETDKLGIK 550 560 LVKTEGAQYI KLTRDGQGAL AFVQQQFD

References

[1]

"Characterization of the lactose-specific enzymes of the phosphotransferase system in Lactococcus lactis." de Vos W.M., Boerrigter I.J., van Rooyen R.J., Reiche B., Hengstenberg W. J. Biol. Chem. 265:22554-22560(1990) [PubMed: 2125052] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], OPERON STRUCTURE, INDUCTION. Strain: MG1820.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60447 Genomic DNA. Translation: AAA25182.1.
PIR	B23696.
3D structure databases
SMR	P23531. Positions 465-565.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.69. 278870.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR003501. PTS_EIIB_2/3. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_LACLA

Accession

Primary (citable) accession number: P23531

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)