Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerate 2-kinase

Gene

garK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.

Caution

E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme.Curated

Catalytic activityi

ATP + D-glycerate = ADP + 2-phospho-D-glycerate.

Kineticsi

  1. KM=51 µM for glycerate2 Publications
  2. KM=70 µM for glycerate2 Publications
  3. KM=61 µM for ATP2 Publications

    pH dependencei

    Optimum pH is 7.3-7.9.2 Publications

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • glycerate 2-kinase activity Source: EcoCyc
    • glycerate kinase activity Source: InterPro

    GO - Biological processi

    • D-glucarate catabolic process Source: EcoCyc
    • galactarate catabolic process Source: EcoCyc
    • organic acid phosphorylation Source: InterPro

    Keywordsi

    Molecular functionKinase, Transferase
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GKI-MONOMER
    MetaCyc:GKI-MONOMER
    BRENDAi2.7.1.165 2165

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerate 2-kinase (EC:2.7.1.165)
    Alternative name(s):
    Glycerate kinase 1
    Short name:
    GK1
    Gene namesi
    Name:garK
    Synonyms:yhaD
    Ordered Locus Names:b3124, JW3093
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11175 garK

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000715321 – 381Glycerate 2-kinaseAdd BLAST381

    Proteomic databases

    PaxDbiP23524
    PRIDEiP23524

    Expressioni

    Inductioni

    In glycerate, glucarate and glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4261538, 11 interactors
    DIPiDIP-28063N
    IntActiP23524, 7 interactors
    STRINGi316385.ECDH10B_3297

    Structurei

    3D structure databases

    ProteinModelPortaliP23524
    SMRiP23524
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycerate kinase type-1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105BZW Bacteria
    COG1929 LUCA
    HOGENOMiHOG000089298
    InParanoidiP23524
    KOiK00865
    OMAiGSATNDC
    PhylomeDBiP23524

    Family and domain databases

    Gene3Di3.40.50.10350, 2 hits
    3.90.1510.10, 1 hit
    InterProiView protein in InterPro
    IPR018193 Glyc_kinase_flavodox-like_fold
    IPR004381 Glycerate_kinase
    IPR018197 Glycerate_kinase_RE-like
    IPR036129 Glycerate_kinase_sf
    PANTHERiPTHR21599 PTHR21599, 1 hit
    PfamiView protein in Pfam
    PF02595 Gly_kinase, 1 hit
    PIRSFiPIRSF006078 GlxK, 1 hit
    SUPFAMiSSF110738 SSF110738, 1 hit
    TIGRFAMsiTIGR00045 TIGR00045, 1 hit

    Sequencei

    Sequence statusi: Complete.

    P23524-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIVIAPDSY KESLSASEVA QAIEKGFREI FPDAQYVSVP VADGGEGTVE
    60 70 80 90 100
    AMIAATQGAE RHAWVTGPLG EKVNASWGIS GDGKTAFIEM AAASGLELVP
    110 120 130 140 150
    AEKRDPLVTT SRGTGELILQ ALESGATNII IGIGGSATND GGAGMVQALG
    160 170 180 190 200
    AKLCDANGNE IGFGGGSLNT LNDIDISGLD PRLKDCVIRV ACDVTNPLVG
    210 220 230 240 250
    DNGASRIFGP QKGASEAMIV ELDNNLSHYA EVIKKALHVD VKDVPGAGAA
    260 270 280 290 300
    GGMGAALMAF LGAELKSGIE IVTTALNLEE HIHDCTLVIT GEGRIDSQSI
    310 320 330 340 350
    HGKVPIGVAN VAKKYHKPVI GIAGSLTDDV GVVHQHGIDA VFSVLTSIGT
    360 370 380
    LDEAFRGAYD NICRASRNIA ATLAIGMRNA G
    Length:381
    Mass (Da):39,104
    Last modified:December 15, 1998 - v2
    Checksum:i0A048E2E5F9FBE32
    GO

    Sequence cautioni

    The sequence AAA57927 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA14239 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAE77171 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90212 Genomic DNA Translation: BAA14239.1 Different initiation.
    U18997 Genomic DNA Translation: AAA57927.1 Different initiation.
    U00096 Genomic DNA Translation: AAC76158.2
    AP009048 Genomic DNA Translation: BAE77171.1 Different initiation.
    PIRiJQ0614
    RefSeqiNP_417593.4, NC_000913.3
    WP_001300387.1, NZ_LN832404.1

    Genome annotation databases

    EnsemblBacteriaiAAC76158; AAC76158; b3124
    BAE77171; BAE77171; BAE77171
    GeneIDi947632
    KEGGiecj:JW3093
    eco:b3124
    PATRICifig|1411691.4.peg.3608

    Similar proteinsi

    Entry informationi

    Entry nameiGLXK1_ECOLI
    AccessioniPrimary (citable) accession number: P23524
    Secondary accession number(s): Q2M985
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: December 15, 1998
    Last modified: March 28, 2018
    This is version 137 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health