Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glycerate 2-kinase

Gene

garK

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-glycerate = ADP + 2-phospho-D-glycerate.

Kineticsi

  1. KM=51 µM for glycerate2 Publications
  2. KM=70 µM for glycerate2 Publications
  3. KM=61 µM for ATP2 Publications

    pH dependencei

    Optimum pH is 7.3-7.9.2 Publications

    GO - Molecular functioni

    • ATP binding Source: UniProtKB-KW
    • glycerate 2-kinase activity Source: EcoCyc
    • glycerate kinase activity Source: InterPro

    GO - Biological processi

    • D-glucarate catabolic process Source: EcoCyc
    • galactarate catabolic process Source: EcoCyc
    • organic acid phosphorylation Source: InterPro
    Complete GO annotation...

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:GKI-MONOMER.
    ECOL316407:JW3093-MONOMER.
    MetaCyc:GKI-MONOMER.
    BRENDAi2.7.1.165. 2165.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerate 2-kinase (EC:2.7.1.165)
    Alternative name(s):
    GK1
    Gene namesi
    Name:garK
    Synonyms:yhaD
    Ordered Locus Names:b3124, JW3093
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    Proteomesi
    • UP000000318 Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    EcoGeneiEG11175. garK.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000715321 – 381Glycerate 2-kinaseAdd BLAST381

    Proteomic databases

    PaxDbiP23524.
    PRIDEiP23524.

    Expressioni

    Inductioni

    In glycerate, glucarate and glycolate-grown cells but not in glucose-grown cells (at protein level).2 Publications

    Interactioni

    Protein-protein interaction databases

    BioGridi4261538. 8 interactors.
    DIPiDIP-28063N.
    IntActiP23524. 7 interactors.
    STRINGi511145.b3124.

    Structurei

    3D structure databases

    ProteinModelPortaliP23524.
    SMRiP23524.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycerate kinase type-1 family.Curated

    Phylogenomic databases

    eggNOGiENOG4105BZW. Bacteria.
    COG1929. LUCA.
    HOGENOMiHOG000089298.
    InParanoidiP23524.
    KOiK00865.
    OMAiNDAGTGM.
    PhylomeDBiP23524.

    Family and domain databases

    Gene3Di3.90.1510.10. 1 hit.
    InterProiIPR018193. Glyc_kinase_flavodox-like-dom.
    IPR004381. Glycerate_kinase.
    [Graphical view]
    PANTHERiPTHR21599. PTHR21599. 1 hit.
    PfamiPF02595. Gly_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006078. GlxK. 1 hit.
    SUPFAMiSSF110738. SSF110738. 1 hit.
    TIGRFAMsiTIGR00045. TIGR00045. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P23524-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MKIVIAPDSY KESLSASEVA QAIEKGFREI FPDAQYVSVP VADGGEGTVE
    60 70 80 90 100
    AMIAATQGAE RHAWVTGPLG EKVNASWGIS GDGKTAFIEM AAASGLELVP
    110 120 130 140 150
    AEKRDPLVTT SRGTGELILQ ALESGATNII IGIGGSATND GGAGMVQALG
    160 170 180 190 200
    AKLCDANGNE IGFGGGSLNT LNDIDISGLD PRLKDCVIRV ACDVTNPLVG
    210 220 230 240 250
    DNGASRIFGP QKGASEAMIV ELDNNLSHYA EVIKKALHVD VKDVPGAGAA
    260 270 280 290 300
    GGMGAALMAF LGAELKSGIE IVTTALNLEE HIHDCTLVIT GEGRIDSQSI
    310 320 330 340 350
    HGKVPIGVAN VAKKYHKPVI GIAGSLTDDV GVVHQHGIDA VFSVLTSIGT
    360 370 380
    LDEAFRGAYD NICRASRNIA ATLAIGMRNA G
    Length:381
    Mass (Da):39,104
    Last modified:December 15, 1998 - v2
    Checksum:i0A048E2E5F9FBE32
    GO

    Sequence cautioni

    The sequence AAA57927 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAA14239 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
    The sequence BAE77171 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90212 Genomic DNA. Translation: BAA14239.1. Different initiation.
    U18997 Genomic DNA. Translation: AAA57927.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76158.2.
    AP009048 Genomic DNA. Translation: BAE77171.1. Different initiation.
    PIRiJQ0614.
    RefSeqiNP_417593.4. NC_000913.3.
    WP_001300387.1. NZ_LN832404.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76158; AAC76158; b3124.
    BAE77171; BAE77171; BAE77171.
    GeneIDi947632.
    KEGGiecj:JW3093.
    eco:b3124.
    PATRICi32121662. VBIEscCol129921_3217.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    D90212 Genomic DNA. Translation: BAA14239.1. Different initiation.
    U18997 Genomic DNA. Translation: AAA57927.1. Different initiation.
    U00096 Genomic DNA. Translation: AAC76158.2.
    AP009048 Genomic DNA. Translation: BAE77171.1. Different initiation.
    PIRiJQ0614.
    RefSeqiNP_417593.4. NC_000913.3.
    WP_001300387.1. NZ_LN832404.1.

    3D structure databases

    ProteinModelPortaliP23524.
    SMRiP23524.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261538. 8 interactors.
    DIPiDIP-28063N.
    IntActiP23524. 7 interactors.
    STRINGi511145.b3124.

    Proteomic databases

    PaxDbiP23524.
    PRIDEiP23524.

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC76158; AAC76158; b3124.
    BAE77171; BAE77171; BAE77171.
    GeneIDi947632.
    KEGGiecj:JW3093.
    eco:b3124.
    PATRICi32121662. VBIEscCol129921_3217.

    Organism-specific databases

    EchoBASEiEB1162.
    EcoGeneiEG11175. garK.

    Phylogenomic databases

    eggNOGiENOG4105BZW. Bacteria.
    COG1929. LUCA.
    HOGENOMiHOG000089298.
    InParanoidiP23524.
    KOiK00865.
    OMAiNDAGTGM.
    PhylomeDBiP23524.

    Enzyme and pathway databases

    BioCyciEcoCyc:GKI-MONOMER.
    ECOL316407:JW3093-MONOMER.
    MetaCyc:GKI-MONOMER.
    BRENDAi2.7.1.165. 2165.

    Miscellaneous databases

    PROiP23524.

    Family and domain databases

    Gene3Di3.90.1510.10. 1 hit.
    InterProiIPR018193. Glyc_kinase_flavodox-like-dom.
    IPR004381. Glycerate_kinase.
    [Graphical view]
    PANTHERiPTHR21599. PTHR21599. 1 hit.
    PfamiPF02595. Gly_kinase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006078. GlxK. 1 hit.
    SUPFAMiSSF110738. SSF110738. 1 hit.
    TIGRFAMsiTIGR00045. TIGR00045. 1 hit.
    ProtoNetiSearch...

    Entry informationi

    Entry nameiGLXK1_ECOLI
    AccessioniPrimary (citable) accession number: P23524
    Secondary accession number(s): Q2M985
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: December 15, 1998
    Last modified: November 2, 2016
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    GK1 has a half-life of 92 minutes while GK2 has a half-life of 11 minutes at 49 degrees Celsius.

    Caution

    E.coli has 2 glycerate kinases, GK1 and GK2; it is not clear which gene encodes which enzyme.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.