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P23511 (NFYA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear transcription factor Y subunit alpha
Alternative name(s):
CAAT box DNA-binding protein subunit A
Nuclear transcription factor Y subunit A
Short name=NF-YA
Gene names
Name:NFYA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length347 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes. Ref.8

Subunit structure

Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with SP1; the interaction is inhibited by glycosylation of SP1. Interacts with ZHX1. Interacts (via N-terminus) with ZHX2 (via homeobox domain). Interacts with ZFX3. Ref.5 Ref.6 Ref.7 Ref.8 Ref.10 Ref.11

Subcellular location

Nucleus.

Sequence similarities

Belongs to the NFYA/HAP2 subunit family.

Contains 1 NFYA/HAP2-type DNA-binding domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Long (identifier: P23511-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Short (identifier: P23511-2)

The sequence of this isoform differs from the canonical sequence as follows:
     26-54: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 347347Nuclear transcription factor Y subunit alpha
PRO_0000198768

Regions

DNA binding296 – 32126NFYA/HAP2-type
Motif266 – 28924Subunit association domain (SAD)
Compositional bias14 – 161148Gln-rich

Amino acid modifications

Modified residue3261Phosphoserine Ref.9

Natural variations

Alternative sequence26 – 5429Missing in isoform Short.
VSP_000849

Experimental info

Sequence conflict2731H → N in AAH39244. Ref.3

Secondary structure

.......... 347
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform Long [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: D00BE17041EB1A9E

FASTA34736,877
        10         20         30         40         50         60 
MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ TLQVVQGQPL 

        70         80         90        100        110        120 
MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG LQQIQLVPPG QIQIQGGQAV 

       130        140        150        160        170        180 
QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ QIAVQGQQVA QTAEGQTIVY QPVNADGTIL 

       190        200        210        220        230        240 
QQVTVPVSGM ITIPAASLAG AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV 

       250        260        270        280        290        300 
PGAGSVPAIQ RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH 

       310        320        330        340 
ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS 

« Hide

Isoform Short [UniParc].

Checksum: 9BF57D6031D83D8C
Show »

FASTA31833,939

References

« Hide 'large scale' references
[1]"Evolutionary variation of the CCAAT-binding transcription factor NF-Y."
Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D.
Nucleic Acids Res. 20:1087-1091(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The DNA sequence and analysis of human chromosome 6."
Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. expand/collapse author list , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
Tissue: Testis.
[4]"A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast."
Becker D.M., Fikes J.D., Guarente L.
Proc. Natl. Acad. Sci. U.S.A. 88:1968-1972(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 91-347.
[5]"Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y."
Yamada K., Printz R.L., Osawa H., Granner D.K.
Biochem. Biophys. Res. Commun. 261:614-621(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZHX1.
[6]"Identification of proteins that interact with NF-YA."
Yamada K., Osawa H., Granner D.K.
FEBS Lett. 460:41-45(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZHX1.
[7]"Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3."
Yamada K., Kawata H., Shou Z., Hirano S., Mizutani T., Yazawa T., Sekiguchi T., Yoshino M., Kajitani T., Miyamoto K.
Biochem. J. 373:167-178(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ZFX3.
Tissue: Testis.
[8]"Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor."
Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K.
Biochem. J. 373:747-757(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH ZHX2.
Tissue: Testis.
[9]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y."
Lim K., Chang H.I.
Biochem. Biophys. Res. Commun. 382:593-597(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH SP1.
[11]"Sequence-specific transcription factor NF-Y displays histone-like DNA binding and H2B-like ubiquitination."
Nardini M., Gnesutta N., Donati G., Gatta R., Forni C., Fossati A., Vonrhein C., Moras D., Romier C., Bolognesi M., Mantovani R.
Cell 152:132-143(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.08 ANGSTROMS) OF 262-332 IN COMPLEX WITH NYFB; NYFC AND PROMOTER DNA, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59711 mRNA. Translation: CAA42231.1.
AL031778 Genomic DNA. Translation: CAB44743.1.
AL031778 Genomic DNA. Translation: CAI20286.1.
BC039244 mRNA. Translation: AAH39244.1.
M59079 mRNA. Translation: AAA35950.1.
CCDSCCDS4849.1. [P23511-1]
CCDS4850.1. [P23511-2]
PIRA39123. S22816.
RefSeqNP_002496.1. NM_002505.4. [P23511-1]
NP_068351.1. NM_021705.3. [P23511-2]
UniGeneHs.10441.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4AWLX-ray3.08A262-332[»]
ProteinModelPortalP23511.
SMRP23511. Positions 262-322.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid110866. 32 interactions.
IntActP23511. 8 interactions.
MINTMINT-98352.
STRING9606.ENSP00000345702.

PTM databases

PhosphoSiteP23511.

Polymorphism databases

DMDM115844.

Proteomic databases

MaxQBP23511.
PaxDbP23511.
PRIDEP23511.

Protocols and materials databases

DNASU4800.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000341376; ENSP00000345702; ENSG00000001167. [P23511-1]
ENST00000353205; ENSP00000229418; ENSG00000001167. [P23511-2]
GeneID4800.
KEGGhsa:4800.
UCSCuc003opo.3. human. [P23511-1]
uc003opp.3. human. [P23511-2]

Organism-specific databases

CTD4800.
GeneCardsGC06P041040.
HGNCHGNC:7804. NFYA.
HPACAB009250.
HPA050779.
MIM189903. gene.
neXtProtNX_P23511.
PharmGKBPA31609.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG5224.
HOGENOMHOG000247016.
HOVERGENHBG005253.
InParanoidP23511.
KOK08064.
OMAQGMITIP.
OrthoDBEOG7MD4RM.
PhylomeDBP23511.
TreeFamTF323257.

Enzyme and pathway databases

ReactomeREACT_111217. Metabolism.
SignaLinkP23511.

Gene expression databases

ArrayExpressP23511.
BgeeP23511.
CleanExHS_NFYA.
GenevestigatorP23511.

Family and domain databases

InterProIPR018362. CCAAT-binding_factor_CS.
IPR001289. TF_CBFB.
[Graphical view]
PANTHERPTHR12632. PTHR12632. 1 hit.
PfamPF02045. CBFB_NFYA. 1 hit.
[Graphical view]
PRINTSPR00616. CCAATSUBUNTB.
SMARTSM00521. CBF. 1 hit.
[Graphical view]
PROSITEPS00686. NFYA_HAP2_1. 1 hit.
PS51152. NFYA_HAP2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNFYA. human.
GeneWikiNFYA.
GenomeRNAi4800.
NextBio18500.
PROP23511.
SOURCESearch...

Entry information

Entry nameNFYA_HUMAN
AccessionPrimary (citable) accession number: P23511
Secondary accession number(s): Q8IXU0
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 1, 1992
Last modified: July 9, 2014
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM