Reviewed, UniProtKB/Swiss-Prot P23511 (NFYA_HUMAN)

Names and origin

Protein names

Recommended name:
    Nuclear transcription factor Y subunit alpha
Alternative name(s):
    Nuclear transcription factor Y subunit A
      Short name=NF-YA
    CAAT-box DNA-binding protein subunit A

Gene names

Name:

NFYA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stimulates the transcription of various genes by recognizing and binding to a CCAAT motif in promoters, for example in type 1 collagen, albumin and beta-actin genes.
Subunit structure	Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the NFYA/HAP2 subunit family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	regulation of transcription, DNA-dependent Inferred from direct assay. Source: UniProtKB transcription from RNA polymerase II promoter Ref.4 Traceable author statement. Source: ProtInc
Cellular component	CCAAT-binding factor complex Inferred from direct assay. Source: UniProtKB
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB transcription factor activity Ref.4 Traceable author statement. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NFYB	P25208	1	EBI-389739,EBI-389728
SFRS1	Q07955	1	EBI-389739,EBI-398920
TP53	P04637	2	EBI-389739,EBI-366083

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Long (identifier: P23511-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: P23511-2)

The sequence of this isoform differs from the canonical sequence as follows:
     26-54: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Nuclear transcription factor Y subunit alpha		PRO_0000198768
Regions
	DNA binding	298 – 318	21	NFYA/HAP2-type
	Motif	266 – 282	17	Subunit association domain (SAD)
	Compositional bias	14 – 161	148	Gln-rich
Amino acid modifications
	Modified residue	326	1	Phosphoserine Ref.5
Natural variations
	Alternative sequence	26 – 54	29	Missing in isoform Short.		VSP_000849
Experimental info
	Sequence conflict	273	1	H → N in AAH39244. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified May 1, 1992. Version 2. Checksum: D00BE17041EB1A9E	FASTA	347	36,877
	10 20 30 40 50 60 MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ TLQVVQGQPL 70 80 90 100 110 120 MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG LQQIQLVPPG QIQIQGGQAV 130 140 150 160 170 180 QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ QIAVQGQQVA QTAEGQTIVY QPVNADGTIL 190 200 210 220 230 240 QQVTVPVSGM ITIPAASLAG AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV 250 260 270 280 290 300 PGAGSVPAIQ RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH 310 320 330 340 ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS
	Isoform Short. Checksum: 9BF57D6031D83D8C	FASTA	318	33,939
	10 20 30 40 50 60 MEQYTANSNS STEQIVVQAG QIQQQVQGQP LMVQVSGGQL ITSTGQPIMV QAVPGGQGQT 70 80 90 100 110 120 IMQVPVSGTQ GLQQIQLVPP GQIQIQGGQA VQVQGQQGQT QQIIIQQPQT AVTAGQTQTQ 130 140 150 160 170 180 QQIAVQGQQV AQTAEGQTIV YQPVNADGTI LQQVTVPVSG MITIPAASLA GAQIVQTGAN 190 200 210 220 230 240 TNTTSSGQGT VTVTLPVAGN VVNSGGMVMM VPGAGSVPAI QRIPLPGAEM LEEEPLYVNA 250 260 270 280 290 300 KQYHRILKRR QARAKLEAEG KIPKERRKYL HESRHRHAMA RKRGEGGRFF SPKEKDSPHM 310 QDPNQADEEA MTQIIRVS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Evolutionary variation of the CCAAT-binding transcription factor NF-Y." Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D. Nucleic Acids Res. 20:1087-1091(1992) [PubMed: 1549471] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT). Tissue: Testis.
[4]	"A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast." Becker D.M., Fikes J.D., Guarente L. Proc. Natl. Acad. Sci. U.S.A. 88:1968-1972(1991) [PubMed: 2000400] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 91-347.
[5]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59711 mRNA. Translation: CAA42231.1. AL031778 Genomic DNA. Translation: CAB44743.1. AL031778 Genomic DNA. Translation: CAI20286.1. BC039244 mRNA. Translation: AAH39244.1. M59079 mRNA. Translation: AAA35950.1.
IPI	IPI00218470. IPI00333568.
PIR	A39123. S22816.
RefSeq	NP_002496.1. NP_068351.1.
UniGene	Hs.10441 Hs.709432 Hs.710402
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P23511. 6 interactions.
PTM databases
PhosphoSite	P23511.
Proteomic databases
PRIDE	P23511.
Genome annotation databases
Ensembl	ENSG00000001167. Homo sapiens. [Contig view]
GeneID	4800.
KEGG	hsa:4800.
UCSC	uc003opo.1. human. uc003opp.1. human.
Organism-specific databases
GeneCards	GC06P041148.
H-InvDB	HIX0005860.
HGNC	HGNC:7804. NFYA.
HPA	CAB009250.
MIM	189903. gene.
PharmGKB	PA31609.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P23511.
HOVERGEN	P23511.
OMA	P23511. AITAGQN.
Gene expression databases
ArrayExpress	P23511.
Bgee	P23511.
CleanEx	HS_NFYA.
GermOnline	ENSG00000001167. Homo sapiens.
Family and domain databases
InterPro	IPR018362. CCAAT-binding_factor_CS. IPR001289. TF_CBFB. [Graphical view]
PANTHER	PTHR12632. TF_CBFB. 1 hit.
Pfam	PF02045. CBFB_NFYA. 1 hit. [Graphical view]
PRINTS	PR00616. CCAATSUBUNTB.
ProDom	PD003860. TF_CBFB. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00521. CBF. 1 hit. [Graphical view]
PROSITE	PS00686. NFYA_HAP2_1. 1 hit. PS51152. NFYA_HAP2_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	18500.
SOURCE	Search...

Entry information

Entry name

NFYA_HUMAN

Accession

Primary (citable) accession number: P23511
Secondary accession number(s): Q8IXU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	May 1, 1992
Last modified:	July 7, 2009
This is version 89 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)