Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23511

- NFYA_HUMAN

UniProt

P23511 - NFYA_HUMAN

Protein

Nuclear transcription factor Y subunit alpha

Gene

NFYA

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 139 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Component of the sequence-specific heterotrimeric transcription factor (NF-Y) which specifically recognizes a 5'-CCAAT-3' box motif found in the promoters of its target genes. NF-Y can function as both an activator and a repressor, depending on its interacting cofactors. NF-YA positively regulates the transcription of the core clock component ARNTL/BMAL1.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi296 – 32126NFYA/HAP2-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. core promoter sequence-specific DNA binding Source: UniProtKB
    2. DNA binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. sequence-specific DNA binding transcription factor activity Source: ProtInc

    GO - Biological processi

    1. cellular lipid metabolic process Source: Reactome
    2. positive regulation of transcription, DNA-templated Source: UniProtKB
    3. positive regulation of transcription from RNA polymerase II promoter Source: Ensembl
    4. regulation of transcription, DNA-templated Source: UniProtKB
    5. small molecule metabolic process Source: Reactome
    6. transcription from RNA polymerase II promoter Source: ProtInc

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Biological rhythms, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Enzyme and pathway databases

    ReactomeiREACT_116145. PPARA activates gene expression.
    REACT_147904. Activation of gene expression by SREBF (SREBP).
    REACT_18355. ATF4 activates genes.
    REACT_18423. ATF6-alpha activates chaperone genes.
    SignaLinkiP23511.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nuclear transcription factor Y subunit alpha
    Alternative name(s):
    CAAT box DNA-binding protein subunit A
    Nuclear transcription factor Y subunit A
    Short name:
    NF-YA
    Gene namesi
    Name:NFYA
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 6

    Organism-specific databases

    HGNCiHGNC:7804. NFYA.

    Subcellular locationi

    GO - Cellular componenti

    1. CCAAT-binding factor complex Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA31609.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 347347Nuclear transcription factor Y subunit alphaPRO_0000198768Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei326 – 3261Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP23511.
    PaxDbiP23511.
    PRIDEiP23511.

    PTM databases

    PhosphoSiteiP23511.

    Expressioni

    Gene expression databases

    ArrayExpressiP23511.
    BgeeiP23511.
    CleanExiHS_NFYA.
    GenevestigatoriP23511.

    Organism-specific databases

    HPAiCAB009250.
    HPA050779.

    Interactioni

    Subunit structurei

    Heterotrimeric transcription factor composed of three components, NF-YA, NF-YB and NF-YC. NF-YB and NF-YC must interact and dimerize for NF-YA association and DNA binding. Interacts with SP1; the interaction is inhibited by glycosylation of SP1. Interacts with ZHX1. Interacts (via N-terminus) with ZHX2 (via homeobox domain). Interacts with ZFX3.6 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    APPBP2Q926243EBI-389739,EBI-743771
    NFYBP252085EBI-389739,EBI-389728
    NFYCQ139524EBI-389739,EBI-389755
    SRSF1Q079555EBI-389739,EBI-398920
    TP53P0463711EBI-389739,EBI-366083

    Protein-protein interaction databases

    BioGridi110866. 32 interactions.
    IntActiP23511. 9 interactions.
    MINTiMINT-98352.
    STRINGi9606.ENSP00000345702.

    Structurei

    Secondary structure

    1
    347
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi264 – 2674
    Turni269 – 2713
    Helixi272 – 28716
    Helixi301 – 3099
    Helixi314 – 3163

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4AWLX-ray3.08A262-332[»]
    ProteinModelPortaliP23511.
    SMRiP23511. Positions 262-322.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi266 – 28924Subunit association domain (SAD)Add
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi14 – 161148Gln-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NFYA/HAP2 subunit family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5224.
    HOGENOMiHOG000247016.
    HOVERGENiHBG005253.
    InParanoidiP23511.
    KOiK08064.
    OMAiQGMITIP.
    OrthoDBiEOG7MD4RM.
    PhylomeDBiP23511.
    TreeFamiTF323257.

    Family and domain databases

    InterProiIPR018362. CCAAT-binding_factor_CS.
    IPR001289. TF_CBFB.
    [Graphical view]
    PANTHERiPTHR12632. PTHR12632. 1 hit.
    PfamiPF02045. CBFB_NFYA. 1 hit.
    [Graphical view]
    PRINTSiPR00616. CCAATSUBUNTB.
    SMARTiSM00521. CBF. 1 hit.
    [Graphical view]
    PROSITEiPS00686. NFYA_HAP2_1. 1 hit.
    PS51152. NFYA_HAP2_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Long (identifier: P23511-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MEQYTANSNS STEQIVVQAG QIQQQQQGGV TAVQLQTEAQ VASASGQQVQ    50
    TLQVVQGQPL MVQVSGGQLI TSTGQPIMVQ AVPGGQGQTI MQVPVSGTQG 100
    LQQIQLVPPG QIQIQGGQAV QVQGQQGQTQ QIIIQQPQTA VTAGQTQTQQ 150
    QIAVQGQQVA QTAEGQTIVY QPVNADGTIL QQVTVPVSGM ITIPAASLAG 200
    AQIVQTGANT NTTSSGQGTV TVTLPVAGNV VNSGGMVMMV PGAGSVPAIQ 250
    RIPLPGAEML EEEPLYVNAK QYHRILKRRQ ARAKLEAEGK IPKERRKYLH 300
    ESRHRHAMAR KRGEGGRFFS PKEKDSPHMQ DPNQADEEAM TQIIRVS 347
    Length:347
    Mass (Da):36,877
    Last modified:May 1, 1992 - v2
    Checksum:iD00BE17041EB1A9E
    GO
    Isoform Short (identifier: P23511-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         26-54: Missing.

    Show »
    Length:318
    Mass (Da):33,939
    Checksum:i9BF57D6031D83D8C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti273 – 2731H → N in AAH39244. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei26 – 5429Missing in isoform Short. 1 PublicationVSP_000849Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59711 mRNA. Translation: CAA42231.1.
    AL031778 Genomic DNA. Translation: CAB44743.1.
    AL031778 Genomic DNA. Translation: CAI20286.1.
    BC039244 mRNA. Translation: AAH39244.1.
    M59079 mRNA. Translation: AAA35950.1.
    CCDSiCCDS4849.1. [P23511-1]
    CCDS4850.1. [P23511-2]
    PIRiS22816. A39123.
    RefSeqiNP_002496.1. NM_002505.4. [P23511-1]
    NP_068351.1. NM_021705.3. [P23511-2]
    UniGeneiHs.10441.

    Genome annotation databases

    EnsembliENST00000341376; ENSP00000345702; ENSG00000001167. [P23511-1]
    ENST00000353205; ENSP00000229418; ENSG00000001167. [P23511-2]
    GeneIDi4800.
    KEGGihsa:4800.
    UCSCiuc003opo.3. human. [P23511-1]
    uc003opp.3. human. [P23511-2]

    Polymorphism databases

    DMDMi115844.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59711 mRNA. Translation: CAA42231.1 .
    AL031778 Genomic DNA. Translation: CAB44743.1 .
    AL031778 Genomic DNA. Translation: CAI20286.1 .
    BC039244 mRNA. Translation: AAH39244.1 .
    M59079 mRNA. Translation: AAA35950.1 .
    CCDSi CCDS4849.1. [P23511-1 ]
    CCDS4850.1. [P23511-2 ]
    PIRi S22816. A39123.
    RefSeqi NP_002496.1. NM_002505.4. [P23511-1 ]
    NP_068351.1. NM_021705.3. [P23511-2 ]
    UniGenei Hs.10441.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4AWL X-ray 3.08 A 262-332 [» ]
    ProteinModelPortali P23511.
    SMRi P23511. Positions 262-322.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 110866. 32 interactions.
    IntActi P23511. 9 interactions.
    MINTi MINT-98352.
    STRINGi 9606.ENSP00000345702.

    PTM databases

    PhosphoSitei P23511.

    Polymorphism databases

    DMDMi 115844.

    Proteomic databases

    MaxQBi P23511.
    PaxDbi P23511.
    PRIDEi P23511.

    Protocols and materials databases

    DNASUi 4800.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000341376 ; ENSP00000345702 ; ENSG00000001167 . [P23511-1 ]
    ENST00000353205 ; ENSP00000229418 ; ENSG00000001167 . [P23511-2 ]
    GeneIDi 4800.
    KEGGi hsa:4800.
    UCSCi uc003opo.3. human. [P23511-1 ]
    uc003opp.3. human. [P23511-2 ]

    Organism-specific databases

    CTDi 4800.
    GeneCardsi GC06P041040.
    HGNCi HGNC:7804. NFYA.
    HPAi CAB009250.
    HPA050779.
    MIMi 189903. gene.
    neXtProti NX_P23511.
    PharmGKBi PA31609.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG5224.
    HOGENOMi HOG000247016.
    HOVERGENi HBG005253.
    InParanoidi P23511.
    KOi K08064.
    OMAi QGMITIP.
    OrthoDBi EOG7MD4RM.
    PhylomeDBi P23511.
    TreeFami TF323257.

    Enzyme and pathway databases

    Reactomei REACT_116145. PPARA activates gene expression.
    REACT_147904. Activation of gene expression by SREBF (SREBP).
    REACT_18355. ATF4 activates genes.
    REACT_18423. ATF6-alpha activates chaperone genes.
    SignaLinki P23511.

    Miscellaneous databases

    ChiTaRSi NFYA. human.
    GeneWikii NFYA.
    GenomeRNAii 4800.
    NextBioi 18500.
    PROi P23511.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P23511.
    Bgeei P23511.
    CleanExi HS_NFYA.
    Genevestigatori P23511.

    Family and domain databases

    InterProi IPR018362. CCAAT-binding_factor_CS.
    IPR001289. TF_CBFB.
    [Graphical view ]
    PANTHERi PTHR12632. PTHR12632. 1 hit.
    Pfami PF02045. CBFB_NFYA. 1 hit.
    [Graphical view ]
    PRINTSi PR00616. CCAATSUBUNTB.
    SMARTi SM00521. CBF. 1 hit.
    [Graphical view ]
    PROSITEi PS00686. NFYA_HAP2_1. 1 hit.
    PS51152. NFYA_HAP2_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Evolutionary variation of the CCAAT-binding transcription factor NF-Y."
      Li X.-Y., Mantovani R., Hooft van Huijsduijnen R., Andre I., Benoist C., Mathis D.
      Nucleic Acids Res. 20:1087-1091(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The DNA sequence and analysis of human chromosome 6."
      Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
      , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
      Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM SHORT).
      Tissue: Testis.
    4. "A cDNA encoding a human CCAAT-binding protein cloned by functional complementation in yeast."
      Becker D.M., Fikes J.D., Guarente L.
      Proc. Natl. Acad. Sci. U.S.A. 88:1968-1972(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 91-347.
    5. "Human ZHX1: cloning, chromosomal location, and interaction with transcription factor NF-Y."
      Yamada K., Printz R.L., Osawa H., Granner D.K.
      Biochem. Biophys. Res. Commun. 261:614-621(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZHX1.
    6. "Identification of proteins that interact with NF-YA."
      Yamada K., Osawa H., Granner D.K.
      FEBS Lett. 460:41-45(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZHX1.
    7. "Analysis of zinc-fingers and homeoboxes (ZHX)-1-interacting proteins: molecular cloning and characterization of a member of the ZHX family, ZHX3."
      Yamada K., Kawata H., Shou Z., Hirano S., Mizutani T., Yazawa T., Sekiguchi T., Yoshino M., Kajitani T., Miyamoto K.
      Biochem. J. 373:167-178(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH ZFX3.
      Tissue: Testis.
    8. "Zinc-fingers and homeoboxes (ZHX) 2, a novel member of the ZHX family, functions as a transcriptional repressor."
      Kawata H., Yamada K., Shou Z., Mizutani T., Yazawa T., Yoshino M., Sekiguchi T., Kajitani T., Miyamoto K.
      Biochem. J. 373:747-757(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH ZHX2.
      Tissue: Testis.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-326, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    10. "O-GlcNAcylation of Sp1 interrupts Sp1 interaction with NF-Y."
      Lim K., Chang H.I.
      Biochem. Biophys. Res. Commun. 382:593-597(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SP1.
    11. "Sequence-specific transcription factor NF-Y displays histone-like DNA binding and H2B-like ubiquitination."
      Nardini M., Gnesutta N., Donati G., Gatta R., Forni C., Fossati A., Vonrhein C., Moras D., Romier C., Bolognesi M., Mantovani R.
      Cell 152:132-143(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.08 ANGSTROMS) OF 262-332 IN COMPLEX WITH NYFB; NYFC AND PROMOTER DNA, SUBUNIT.

    Entry informationi

    Entry nameiNFYA_HUMAN
    AccessioniPrimary (citable) accession number: P23511
    Secondary accession number(s): Q8IXU0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 139 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 6
      Human chromosome 6: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3