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P23508

- CRCM_HUMAN

UniProt

P23508 - CRCM_HUMAN

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Protein

Colorectal mutant cancer protein

Gene

MCC

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Candidate for the putative colorectal tumor suppressor gene located at 5q21. Suppresses cell proliferation and the Wnt/b-catenin pathway in colorectal cancer cells. Inhibits DNA binding of b-catenin/TCF/LEF transcription factors. Involved in cell migration independently of RAC1, CDC42 and p21-activated kinase (PAK) activation.3 Publications

GO - Molecular functioni

  1. receptor activity Source: ProtInc

GO - Biological processi

  1. negative regulation of canonical Wnt signaling pathway Source: UniProtKB
  2. negative regulation of epithelial cell migration Source: UniProtKB
  3. negative regulation of epithelial cell proliferation Source: UniProtKB
  4. signal transduction Source: ProtInc
  5. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Wnt signaling pathway

Enzyme and pathway databases

SignaLinkiP23508.

Names & Taxonomyi

Protein namesi
Recommended name:
Colorectal mutant cancer protein
Short name:
Protein MCC
Gene namesi
Name:MCC
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:6935. MCC.

Subcellular locationi

Cell membrane. Cell projectionlamellipodium. Nucleus. Cytoplasm
Note: Colocalizes with actin at the leading edge of polarized cells.

GO - Cellular componenti

  1. cell projection Source: UniProtKB-KW
  2. cytoplasm Source: UniProtKB
  3. nucleus Source: UniProtKB
  4. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi828 – 8281S → A: Reduced binding to SCRIB. 1 Publication
Mutagenesisi828 – 8281S → D: Higher membrane localization, reduced formation of lamellipodia, accumulation of MYH10 at the cell cortex. 1 Publication

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

PharmGKBiPA30679.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 829829Colorectal mutant cancer proteinPRO_0000079333Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei828 – 8281Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP23508.
PaxDbiP23508.
PRIDEiP23508.

PTM databases

PhosphoSiteiP23508.

Expressioni

Tissue specificityi

Expressed in a variety of tissues.

Gene expression databases

BgeeiP23508.
CleanExiHS_MCC.
ExpressionAtlasiP23508. baseline and differential.
GenevestigatoriP23508.

Organism-specific databases

HPAiHPA037390.
HPA037391.

Interactioni

Subunit structurei

Interacts with SCRIB (via phosphorylated PDZ-binding motif), EZR, SNX27, SLC9A3R1 and SLC9A3R2. Interacts with CTNNB1; the interaction is enhanced upon Wnt stimulation. Interacts with MYH10.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
GTF2E2P290842EBI-307531,EBI-2853321
SCRIBQ141608EBI-307531,EBI-357345

Protein-protein interaction databases

BioGridi110333. 44 interactions.
DIPiDIP-27599N.
IntActiP23508. 13 interactions.
MINTiMINT-1161194.
STRINGi9606.ENSP00000386227.

Structurei

3D structure databases

ProteinModelPortaliP23508.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi826 – 8294PDZ-binding

Sequence similaritiesi

Belongs to the MCC family.Curated

Phylogenomic databases

eggNOGiNOG122105.
GeneTreeiENSGT00530000063974.
HOGENOMiHOG000111967.
HOVERGENiHBG004762.
InParanoidiP23508.
OMAiPGKECAD.
OrthoDBiEOG7B05C9.
PhylomeDBiP23508.
TreeFamiTF333056.

Family and domain databases

InterProiIPR019536. USH1C-bd_PDZ_domain.
[Graphical view]
PfamiPF10506. MCC-bdg_PDZ. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P23508-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNSGVAMKYG NDSSAELSEL HSAALASLKG DIVELNKRLQ QTERERDLLE
60 70 80 90 100
KKLAKAQCEQ SHLMREHEDV QERTTLRYEE RITELHSVIA ELNKKIDRLQ
110 120 130 140 150
GTTIREEDEY SELRSELSQS QHEVNEDSRS MDQDQTSVSI PENQSTMVTA
160 170 180 190 200
DMDNCSDLNS ELQRVLTGLE NVVCGRKKSS CSLSVAEVDK HIEQLTTASE
210 220 230 240 250
HCDLAIKTVE EIEGVLGRDL YPNLAEERSR WEKELAGLRE ENESLTAMLC
260 270 280 290 300
SKEEELNRTK ATMNAIREER DRLRRRVREL QTRLQSVQAT GPSSPGRLTS
310 320 330 340 350
TNRPINPSTG ELSTSSSSND IPIAKIAERV KLSKTRSESS SSDRPVLGSE
360 370 380 390 400
ISSIGVSSSV AEHLAHSLQD CSNIQEIFQT LYSHGSAISE SKIREFEVET
410 420 430 440 450
ERLNSRIEHL KSQNDLLTIT LEECKSNAER MSMLVGKYES NATALRLALQ
460 470 480 490 500
YSEQCIEAYE LLLALAESEQ SLILGQFRAA GVGSSPGDQS GDENITQMLK
510 520 530 540 550
RAHDCRKTAE NAAKALLMKL DGSCGGAFAV AGCSVQPWES LSSNSHTSTT
560 570 580 590 600
SSTASSCDTE FTKEDEQRLK DYIQQLKNDR AAVKLTMLEL ESIHIDPLSY
610 620 630 640 650
DVKPRGDSQR LDLENAVLMQ ELMAMKEEMA ELKAQLYLLE KEKKALELKL
660 670 680 690 700
STREAQEQAY LVHIEHLKSE VEEQKEQRMR SLSSTSSGSK DKPGKECADA
710 720 730 740 750
ASPALSLAEL RTTCSENELA AEFTNAIRRE KKLKARVQEL VSALERLTKS
760 770 780 790 800
SEIRHQQSAE FVNDLKRANS NLVAAYEKAK KKHQNKLKKL ESQMMAMVER
810 820
HETQVRMLKQ RIALLEEENS RPHTNETSL
Length:829
Mass (Da):93,027
Last modified:January 11, 2011 - v2
Checksum:iC90611B2E91A7F60
GO
Isoform 2 (identifier: P23508-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MNSGVAMKYGNDSSAELSE → MMAAAAAAAA...GGSYLELANT

Show »
Length:1,019
Mass (Da):113,279
Checksum:i09C330B8B43832F1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti353 – 3531S → G in BAC87521. (PubMed:14702039)Curated
Isoform 2 (identifier: P23508-2)
Sequence conflicti25 – 251S → GSSSG in BAC87521. (PubMed:14702039)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti190 – 1901K → R.3 Publications
Corresponds to variant rs6594681 [ dbSNP | Ensembl ].
VAR_050905
Natural varianti267 – 2671R → L in a colorectal cancer sample. 1 Publication
VAR_005141
Natural varianti486 – 4861P → L in a colorectal cancer sample. 1 Publication
Corresponds to variant rs35269015 [ dbSNP | Ensembl ].
VAR_005142
Natural varianti490 – 4901S → L in a colorectal cancer sample. 1 Publication
VAR_005143
Natural varianti506 – 5061R → Q in colorectal cancer samples; somatic mutation. 2 Publications
VAR_005144
Natural varianti698 – 6981A → V in colorectal cancer samples; somatic mutation. 2 Publications
VAR_005145
Natural varianti751 – 7511S → C.
Corresponds to variant rs17313892 [ dbSNP | Ensembl ].
VAR_033753

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919MNSGV…AELSE → MMAAAAAAAAGSSSSGGGGG GSGSSSSSSDTSSTGEEERM RRLFQTCDGDGDGYISRNDL LMVCRQLNMEESVAEIMNQL GADENGKISFQDFTRCRMQL VREIRKEEVDLSAKSDNSCT KKLRDRIASWPTSSDNSLGA LSAARESWEYDSGARDLQSP DVQSQSALQKLLEYGGSSLH QQAALHKLLTQSPHIGNSVG GSYLELANT in isoform 2. 1 PublicationVSP_037660Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62397 mRNA. Translation: AAA52069.1.
AC008536 Genomic DNA. No translation available.
AC010431 Genomic DNA. No translation available.
AC079465 Genomic DNA. No translation available.
AC093208 Genomic DNA. No translation available.
AC106750 Genomic DNA. No translation available.
AC126917 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW48984.1.
CH471086 Genomic DNA. Translation: EAW48986.1.
BC009279 mRNA. Translation: AAH09279.1.
BC018919 mRNA. Translation: AAH18919.1.
AK128596 mRNA. Translation: BAC87521.1.
CCDSiCCDS4111.1. [P23508-1]
CCDS43351.1. [P23508-2]
PIRiA38434. A33166.
RefSeqiNP_001078846.1. NM_001085377.1.
NP_002378.1. NM_002387.2.
UniGeneiHs.593171.

Genome annotation databases

EnsembliENST00000302475; ENSP00000305617; ENSG00000171444. [P23508-1]
ENST00000408903; ENSP00000386227; ENSG00000171444. [P23508-2]
GeneIDi4163.
KEGGihsa:4163.
UCSCiuc003kqj.4. human. [P23508-1]
uc003kql.4. human. [P23508-2]

Polymorphism databases

DMDMi317373352.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62397 mRNA. Translation: AAA52069.1 .
AC008536 Genomic DNA. No translation available.
AC010431 Genomic DNA. No translation available.
AC079465 Genomic DNA. No translation available.
AC093208 Genomic DNA. No translation available.
AC106750 Genomic DNA. No translation available.
AC126917 Genomic DNA. No translation available.
CH471086 Genomic DNA. Translation: EAW48984.1 .
CH471086 Genomic DNA. Translation: EAW48986.1 .
BC009279 mRNA. Translation: AAH09279.1 .
BC018919 mRNA. Translation: AAH18919.1 .
AK128596 mRNA. Translation: BAC87521.1 .
CCDSi CCDS4111.1. [P23508-1 ]
CCDS43351.1. [P23508-2 ]
PIRi A38434. A33166.
RefSeqi NP_001078846.1. NM_001085377.1.
NP_002378.1. NM_002387.2.
UniGenei Hs.593171.

3D structure databases

ProteinModelPortali P23508.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 110333. 44 interactions.
DIPi DIP-27599N.
IntActi P23508. 13 interactions.
MINTi MINT-1161194.
STRINGi 9606.ENSP00000386227.

PTM databases

PhosphoSitei P23508.

Polymorphism databases

DMDMi 317373352.

Proteomic databases

MaxQBi P23508.
PaxDbi P23508.
PRIDEi P23508.

Protocols and materials databases

DNASUi 4163.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000302475 ; ENSP00000305617 ; ENSG00000171444 . [P23508-1 ]
ENST00000408903 ; ENSP00000386227 ; ENSG00000171444 . [P23508-2 ]
GeneIDi 4163.
KEGGi hsa:4163.
UCSCi uc003kqj.4. human. [P23508-1 ]
uc003kql.4. human. [P23508-2 ]

Organism-specific databases

CTDi 4163.
GeneCardsi GC05M112388.
HGNCi HGNC:6935. MCC.
HPAi HPA037390.
HPA037391.
MIMi 159350. gene.
neXtProti NX_P23508.
PharmGKBi PA30679.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG122105.
GeneTreei ENSGT00530000063974.
HOGENOMi HOG000111967.
HOVERGENi HBG004762.
InParanoidi P23508.
OMAi PGKECAD.
OrthoDBi EOG7B05C9.
PhylomeDBi P23508.
TreeFami TF333056.

Enzyme and pathway databases

SignaLinki P23508.

Miscellaneous databases

ChiTaRSi MCC. human.
GeneWikii MCC_(gene).
GenomeRNAii 4163.
NextBioi 16402.
PROi P23508.
SOURCEi Search...

Gene expression databases

Bgeei P23508.
CleanExi HS_MCC.
ExpressionAtlasi P23508. baseline and differential.
Genevestigatori P23508.

Family and domain databases

InterProi IPR019536. USH1C-bd_PDZ_domain.
[Graphical view ]
Pfami PF10506. MCC-bdg_PDZ. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS ARG-190; GLN-506 AND VAL-698.
    Tissue: Brain.
  2. "The DNA sequence and comparative analysis of human chromosome 5."
    Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S., Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M., She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.
    , Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R., Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J., Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A., Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.
    Nature 431:268-274(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT ARG-190.
    Tissue: Muscle.
  5. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-779 (ISOFORM 2), VARIANT ARG-190.
    Tissue: Trachea.
  6. "Mutated in colorectal cancer, a putative tumor suppressor for serrated colorectal cancer, selectively represses beta-catenin-dependent transcription."
    Fukuyama R., Niculaita R., Ng K.P., Obusez E., Sanchez J., Kalady M., Aung P.P., Casey G., Sizemore N.
    Oncogene 27:6044-6055(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN WNT SIGNALING, SUBCELLULAR LOCATION, INTERACTION WITH CTNNB1.
  7. "MCC, a new interacting protein for Scrib, is required for cell migration in epithelial cells."
    Arnaud C., Sebbagh M., Nola S., Audebert S., Bidaut G., Hermant A., Gayet O., Dusetti N.J., Ollendorff V., Santoni M.J., Borg J.P., Lecine P.
    FEBS Lett. 583:2326-2332(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN CELL MIGRATION, SUBCELLULAR LOCATION, INTERACTION WITH SCRIB; EZR; SNX27; SLC9A3R1 AND SLC9A3R2.
  8. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  9. "The PDZ-binding motif of MCC is phosphorylated at position -1 and controls lamellipodia formation in colon epithelial cells."
    Pangon L., Van Kralingen C., Abas M., Daly R.J., Musgrove E.A., Kohonen-Corish M.R.
    Biochim. Biophys. Acta 1823:1058-1067(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PDZ-BINDING MOTIF, PHOSPHORYLATION AT SER-828, INTERACTION WITH SCRIB AND MYH10, SUBCELLULAR LOCATION, MUTAGENESIS OF SER-828.
  10. Cited for: VARIANTS LEU-267; LEU-486; LEU-490; GLN-506 AND VAL-698.

Entry informationi

Entry nameiCRCM_HUMAN
AccessioniPrimary (citable) accession number: P23508
Secondary accession number(s): D3DT05, Q6ZR04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 11, 2011
Last modified: November 26, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3