Reviewed, UniProtKB/Swiss-Prot P23506 (PIMT_MOUSE)

Names and origin

Protein names

Recommended name:
    Protein-L-isoaspartate(D-aspartate) O-methyltransferase
      Short name=PIMT
    EC=2.1.1.77
Alternative name(s):
    Protein-beta-aspartate methyltransferase
    Protein L-isoaspartyl/D-aspartyl methyltransferase
    L-isoaspartyl protein carboxyl methyltransferase

Gene names

Name:

Pcmt1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	protein amino acid methylation Inferred from direct assay. Source: MGI
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from direct assay. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P23506-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P23506-2)

The sequence of this isoform differs from the canonical sequence as follows:
     226-227: WK → DEL

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 227	226	Protein-L-isoaspartate(D-aspartate) O-methyltransferase		PRO_0000111876
Sites
	Active site	60	1	By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylalanine By similarity
Natural variations
	Alternative sequence	226 – 227	2	WK → DEL in isoform 2.		VSP_004717

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 954E2491A9DA9249	FASTA	227	24,634
	10 20 30 40 50 60 MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKSNPYMDSP QSIGFQATIS 70 80 90 100 110 120 APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV GNSGKVIGID HIKELVDDSI 130 140 150 160 170 180 TNVKKDDPML LSSGRVRLVV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI 190 200 210 220 LPVGPAGGNQ MLEQYDKLQD GSVKMKPLMG VIYVPLTDKE KQWSRWK
	Isoform 2. Checksum: 07ED77F491A9DA92	FASTA	228	24,677
	10 20 30 40 50 60 MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKSNPYMDSP QSIGFQATIS 70 80 90 100 110 120 APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV GNSGKVIGID HIKELVDDSI 130 140 150 160 170 180 TNVKKDDPML LSSGRVRLVV GDGRMGYAEE APYDAIHVGA AAPVVPQALI DQLKPGGRLI 190 200 210 220 LPVGPAGGNQ MLEQYDKLQD GSVKMKPLMG VIYVPLTDKE KQWSRDEL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization and tissue expression of the murine gene encoding the protein beta-aspartate methyltransferase." Romanik E.A., Ladino C.A., Killoy L.C., D'Ardenne S.C., O'Connor C.M. Gene 118:217-222(1992) [PubMed: 1511895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: C57BL/6. Tissue: Brain and Testis.
[3]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 82-98, MASS SPECTROMETRY. Tissue: Hippocampus.
[4]	"Structural analysis of transcripts for the protein L-isoaspartyl methyltransferase reveals multiple transcription initiation sites and a distinct pattern of expression in mouse testis: identification of a 5'-flanking sequence with promoter activity." Galus A., Lagos A., Romanik E.A., O'Connor C.M. Arch. Biochem. Biophys. 312:524-533(1994) [PubMed: 8037467] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 186-227 (ISOFORM 2).
[5]	"Proteomic identification of novel substrates of a protein isoaspartyl methyltransferase repair enzyme." Vigneswara V., Lowenson J.D., Powell C.D., Thakur M., Bailey K., Clarke S., Ray D.E., Carter W.G. J. Biol. Chem. 281:32619-32629(2006) [PubMed: 16923807] [Abstract] Cited for: SUBSTRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60320 mRNA. Translation: AAA92742.1. Sequence problems. M84684 Genomic DNA. Translation: AAA74565.1. BC049613 mRNA. Translation: AAH49613.1. Different initiation. BC058966 mRNA. Translation: AAH58966.1. S72473 mRNA. Translation: AAB31369.1.
IPI	IPI00329913. IPI00757109.
PIR	JC1248.
UniGene	Mm.258431
3D structure databases
HSSP	HSSP built from PDB template 1I1N based on UniProtKB P22061.
SMR	P23506. Positions 3-226.
ModBase	Search...
2-D gel databases
REPRODUCTION-2DPAGE	P23506.
Proteomic databases
PRIDE	P23506.
Genome annotation databases
Ensembl	ENSMUSG00000019795. Mus musculus. [Contig view]
KEGG	mmu:18537.
Organism-specific databases
MGI	MGI:97502. Pcmt1.
Phylogenomic databases
HOGENOM	P23506.
HOVERGEN	P23506.
OMA	P23506. EKQWSRW.
Enzyme and pathway databases
BRENDA	2.1.1.77. 244.
Gene expression databases
ArrayExpress	P23506.
Bgee	P23506.
GermOnline	ENSMUSG00000019795. Mus musculus.
Family and domain databases
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PCMT. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	294308.
SOURCE	Search...

Entry information

Entry name

PIMT_MOUSE

Accession

Primary (citable) accession number: P23506
Secondary accession number(s): Q810R4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 81 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)