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Protein

Protein-L-isoaspartate(D-aspartate) O-methyltransferase

Gene

Pcmt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Acts on EIF4EBP2, microtubule-associated protein 2, calreticulin, clathrin light chains a and b, Ubiquitin carboxyl-terminal hydrolase isozyme L1, phosphatidylethanolamine-binding protein 1, stathmin, beta-synuclein and alpha-synuclein.2 Publications

Catalytic activityi

S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei60By similarity1

GO - Molecular functioni

  • protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Source: MGI

GO - Biological processi

  • protein methylation Source: MGI

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-L-isoaspartate(D-aspartate) O-methyltransferase (EC:2.1.1.77)
Short name:
PIMT
Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl/D-aspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Gene namesi
Name:Pcmt1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97502 Pcmt1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001118762 – 227Protein-L-isoaspartate(D-aspartate) O-methyltransferaseAdd BLAST226

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP23506
MaxQBiP23506
PaxDbiP23506
PRIDEiP23506

2D gel databases

REPRODUCTION-2DPAGEiP23506

PTM databases

iPTMnetiP23506
PhosphoSitePlusiP23506

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP23506, 2 interactors
MINTiP23506
STRINGi10090.ENSMUSP00000124932

Structurei

3D structure databases

ProteinModelPortaliP23506
SMRiP23506
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1661 Eukaryota
COG2518 LUCA
HOGENOMiHOG000257189
HOVERGENiHBG004483
InParanoidiP23506

Family and domain databases

InterProiView protein in InterPro
IPR000682 PCMT
IPR029063 SAM-dependent_MTases
PANTHERiPTHR11579 PTHR11579, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00080 pimt, 1 hit
PROSITEiView protein in PROSITE
PS01279 PCMT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P23506-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAWKSGGASH SELIHNLRKN GIIKTDKVFE VMLATDRSHY AKSNPYMDSP
60 70 80 90 100
QSIGFQATIS APHMHAYALE LLFDQLHEGA KALDVGSGSG ILTACFARMV
110 120 130 140 150
GNSGKVIGID HIKELVDDSI TNVKKDDPML LSSGRVRLVV GDGRMGYAEE
160 170 180 190 200
APYDAIHVGA AAPVVPQALI DQLKPGGRLI LPVGPAGGNQ MLEQYDKLQD
210 220
GSVKMKPLMG VIYVPLTDKE KQWSRWK
Length:227
Mass (Da):24,634
Last modified:January 23, 2007 - v3
Checksum:i954E2491A9DA9249
GO
Isoform 2 (identifier: P23506-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     226-227: WK → DEL

Show »
Length:228
Mass (Da):24,677
Checksum:i07ED77F491A9DA92
GO

Sequence cautioni

The sequence AAH49613 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004717226 – 227WK → DEL in isoform 2. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60320 mRNA Translation: AAA92742.1 Sequence problems.
M84684 Genomic DNA Translation: AAA74565.1
BC049613 mRNA Translation: AAH49613.1 Different initiation.
BC058966 mRNA Translation: AAH58966.1
S72473 mRNA Translation: AAB31369.1
PIRiJC1248
UniGeneiMm.258431
Mm.394947

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiPIMT_MOUSE
AccessioniPrimary (citable) accession number: P23506
Secondary accession number(s): Q810R4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: January 23, 2007
Last modified: February 28, 2018
This is version 141 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
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Main funding by: National Institutes of Health