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Protein

Purine nucleoside phosphorylase

Gene

Pnp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.1 Publication

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

Pathwayi: purine nucleoside salvage

This protein is involved in the pathway purine nucleoside salvage, which is part of Purine metabolism.
View all proteins of this organism that are known to be involved in the pathway purine nucleoside salvage and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33PhosphateBy similarity1
Binding sitei64PhosphateBy similarity1
Binding sitei116Phosphate; via amide nitrogenBy similarity1
Binding sitei201Purine nucleosideBy similarity1
Binding sitei220PhosphateBy similarity1
Binding sitei243Purine nucleosideBy similarity1

GO - Molecular functioni

GO - Biological processi

  • apoptotic mitochondrial changes Source: MGI
  • deoxyguanosine catabolic process Source: MGI
  • deoxyinosine catabolic process Source: MGI
  • dGTP metabolic process Source: MGI
  • GTP biosynthetic process Source: MGI
  • guanosine catabolic process Source: MGI
  • inosine catabolic process Source: MGI
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • negative regulation of T cell apoptotic process Source: MGI
  • positive regulation of alpha-beta T cell differentiation Source: MGI
  • positive regulation of B cell differentiation Source: MGI
  • positive regulation of DNA repair Source: MGI
  • positive regulation of T cell mediated cytotoxicity Source: MGI
  • positive regulation of T cell proliferation Source: MGI
  • purine nucleoside metabolic process Source: MGI
  • response to gamma radiation Source: MGI
  • urate biosynthetic process Source: MGI

Keywordsi

Molecular functionGlycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00606

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase (EC:2.4.2.1)
Short name:
PNP
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Gene namesi
Name:Pnp
Synonyms:Np, Pnp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97365 Pnp

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2215

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001845371 – 289Purine nucleoside phosphorylaseAdd BLAST289

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP23492
MaxQBiP23492
PaxDbiP23492
PeptideAtlasiP23492
PRIDEiP23492

PTM databases

iPTMnetiP23492
PhosphoSitePlusiP23492
SwissPalmiP23492

Expressioni

Gene expression databases

CleanExiMM_PNP1

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

BioGridi202284, 1 interactor
IntActiP23492, 3 interactors
MINTiP23492
STRINGi10090.ENSMUSP00000043926

Chemistry databases

BindingDBiP23492

Structurei

3D structure databases

ProteinModelPortaliP23492
SMRiP23492
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni84 – 86Phosphate bindingBy similarity3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family.Curated

Phylogenomic databases

eggNOGiKOG3984 Eukaryota
COG0005 LUCA
HOGENOMiHOG000045183
HOVERGENiHBG002460
InParanoidiP23492
KOiK03783
PhylomeDBiP23492

Family and domain databases

InterProiView protein in InterPro
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR011270 Pur_Nuc_Pase_Ino/Guo-sp
IPR011268 Purine_phosphorylase
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR11904 PTHR11904, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
PIRSFiPIRSF000477 PurNPase, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01700 PNPH, 1 hit
TIGR01697 PNPH-PUNA-XAPA, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

P23492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENEFTYEDY ETTAKWLLQH TEYRPQVAVI CGSGLGGLTA HLKEAQIFDY
60 70 80 90 100
NEIPNFPQST VQGHAGRLVF GLLNGRCCVM MQGRFHMYEG YSLSKVTFPV
110 120 130 140 150
RVFHLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FCGQNPLRGP
160 170 180 190 200
NDERFGVRFP AMSDAYDRDM RQKAFTAWKQ MGEQRKLQEG TYVMLAGPNF
210 220 230 240 250
ETVAESRLLK MLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVVMDYE
260 270 280
NLEKANHMEV LDAGKAAAQT LERFVSILME SIPLPDRGS
Length:289
Mass (Da):32,277
Last modified:February 1, 1996 - v2
Checksum:iDE7C1C2B004A1113
GO

Polymorphismi

Four electrophoretic alleles of NP are known; NPA (shown here), NPB, NPC and NPD.

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti22E → K in haplotype NPD. 1
Natural varianti39T → A in haplotype NPD. 1
Natural varianti152D → E in haplotype NPD. 1
Natural varianti176T → S in haplotype NPB. 1
Natural varianti258M → K in haplotype NPC. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56548 mRNA Translation: CAA39888.1
M84563 mRNA Translation: AAA39835.1
L11290 mRNA Translation: AAC37634.1
L11291 mRNA Translation: AAC37635.1
L11292 mRNA Translation: AAC37706.1
U35374 mRNA Translation: AAB60510.1
CT010316 mRNA Translation: CAJ18524.1
BC003788 mRNA Translation: AAH03788.1
BC052679 mRNA Translation: AAH52679.1
CCDSiCCDS27029.1
PIRiI57010
I76672
RefSeqiNP_038660.1, NM_013632.4
UniGeneiMm.17932

Genome annotation databases

GeneIDi18950
KEGGimmu:18950

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiPNPH_MOUSE
AccessioniPrimary (citable) accession number: P23492
Secondary accession number(s): Q4FJT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: June 20, 2018
This is version 147 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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