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Protein

Purine nucleoside phosphorylase

Gene

Pnp

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.1 Publication

Catalytic activityi

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

Pathwayi

GO - Molecular functioni

  1. drug binding Source: MGI
  2. nucleoside binding Source: MGI
  3. phosphate ion binding Source: MGI
  4. purine nucleobase binding Source: MGI
  5. purine-nucleoside phosphorylase activity Source: MGI

GO - Biological processi

  1. apoptotic mitochondrial changes Source: MGI
  2. deoxyguanosine catabolic process Source: MGI
  3. deoxyinosine catabolic process Source: MGI
  4. dGTP metabolic process Source: MGI
  5. GTP biosynthetic process Source: MGI
  6. guanosine catabolic process Source: MGI
  7. inosine catabolic process Source: MGI
  8. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  9. negative regulation of T cell apoptotic process Source: MGI
  10. positive regulation of alpha-beta T cell differentiation Source: MGI
  11. positive regulation of B cell differentiation Source: MGI
  12. positive regulation of DNA repair Source: MGI
  13. positive regulation of T cell mediated cytotoxicity Source: MGI
  14. positive regulation of T cell proliferation Source: MGI
  15. purine nucleoside metabolic process Source: MGI
  16. response to gamma radiation Source: MGI
  17. urate biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00606.

Names & Taxonomyi

Protein namesi
Recommended name:
Purine nucleoside phosphorylase (EC:2.4.2.1)
Short name:
PNP
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Gene namesi
Name:Pnp
Synonyms:Np, Pnp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97365. Pnp.

Subcellular locationi

Cytoplasmcytoskeleton By similarity

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytoskeleton Source: UniProtKB-SubCell
  3. cytosol Source: MGI
  4. extracellular vesicular exosome Source: MGI
  5. intracellular Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Purine nucleoside phosphorylasePRO_0000184537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP23492.
PaxDbiP23492.
PRIDEiP23492.

PTM databases

PhosphoSiteiP23492.

Expressioni

Gene expression databases

CleanExiMM_PNP1.
GenevestigatoriP23492.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

IntActiP23492. 3 interactions.
MINTiMINT-1869537.

Structurei

3D structure databases

ProteinModelPortaliP23492.
SMRiP23492. Positions 3-282.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family.Curated

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000045183.
HOVERGENiHBG002460.
InParanoidiP23492.
KOiK03783.
PhylomeDBiP23492.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENEFTYEDY ETTAKWLLQH TEYRPQVAVI CGSGLGGLTA HLKEAQIFDY
60 70 80 90 100
NEIPNFPQST VQGHAGRLVF GLLNGRCCVM MQGRFHMYEG YSLSKVTFPV
110 120 130 140 150
RVFHLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FCGQNPLRGP
160 170 180 190 200
NDERFGVRFP AMSDAYDRDM RQKAFTAWKQ MGEQRKLQEG TYVMLAGPNF
210 220 230 240 250
ETVAESRLLK MLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVVMDYE
260 270 280
NLEKANHMEV LDAGKAAAQT LERFVSILME SIPLPDRGS
Length:289
Mass (Da):32,277
Last modified:January 31, 1996 - v2
Checksum:iDE7C1C2B004A1113
GO

Polymorphismi

Four electrophoretic alleles of NP are known; NPA (shown here), NPB, NPC and NPD.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti22 – 221E → K in haplotype NPD.
Natural varianti39 – 391T → A in haplotype NPD.
Natural varianti152 – 1521D → E in haplotype NPD.
Natural varianti176 – 1761T → S in haplotype NPB.
Natural varianti258 – 2581M → K in haplotype NPC.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56548 mRNA. Translation: CAA39888.1.
M84563 mRNA. Translation: AAA39835.1.
L11290 mRNA. Translation: AAC37634.1.
L11291 mRNA. Translation: AAC37635.1.
L11292 mRNA. Translation: AAC37706.1.
U35374 mRNA. Translation: AAB60510.1.
CT010316 mRNA. Translation: CAJ18524.1.
BC003788 mRNA. Translation: AAH03788.1.
BC052679 mRNA. Translation: AAH52679.1.
CCDSiCCDS27029.1.
PIRiI57010.
I76672.
RefSeqiNP_038660.1. NM_013632.4.
UniGeneiMm.17932.

Genome annotation databases

GeneIDi18950.
KEGGimmu:18950.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X56548 mRNA. Translation: CAA39888.1.
M84563 mRNA. Translation: AAA39835.1.
L11290 mRNA. Translation: AAC37634.1.
L11291 mRNA. Translation: AAC37635.1.
L11292 mRNA. Translation: AAC37706.1.
U35374 mRNA. Translation: AAB60510.1.
CT010316 mRNA. Translation: CAJ18524.1.
BC003788 mRNA. Translation: AAH03788.1.
BC052679 mRNA. Translation: AAH52679.1.
CCDSiCCDS27029.1.
PIRiI57010.
I76672.
RefSeqiNP_038660.1. NM_013632.4.
UniGeneiMm.17932.

3D structure databases

ProteinModelPortaliP23492.
SMRiP23492. Positions 3-282.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP23492. 3 interactions.
MINTiMINT-1869537.

Chemistry

BindingDBiP23492.
ChEMBLiCHEMBL2215.

PTM databases

PhosphoSiteiP23492.

Proteomic databases

MaxQBiP23492.
PaxDbiP23492.
PRIDEiP23492.

Protocols and materials databases

DNASUi18950.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18950.
KEGGimmu:18950.

Organism-specific databases

CTDi4860.
MGIiMGI:97365. Pnp.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000045183.
HOVERGENiHBG002460.
InParanoidiP23492.
KOiK03783.
PhylomeDBiP23492.

Enzyme and pathway databases

UniPathwayiUPA00606.

Miscellaneous databases

NextBioi295296.
PROiP23492.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PNP1.
GenevestigatoriP23492.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
InterProiIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFiPIRSF000477. PurNPase. 1 hit.
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of murine purine nucleoside phosphorylase cDNA."
    Jenuth J.P., Snyder F.F.
    Nucleic Acids Res. 19:1708-1708(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
  2. "Isolation and expression of a murine purine nucleoside phosphorylase-encoding cDNA and sequence similarity with the human message."
    Nelson D.M., Foresman M.D., Ronnei B.J., McIvor R.S.
    Gene 113:215-221(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  3. "cDNA sequence of four purine nucleoside phosphorylase (Np) alleles in the mouse."
    Jenuth J.P., Mangat R.K., Snyder F.F.
    Mamm. Genome 4:598-603(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HEHA, MOLF/Ei and SPRET-1.
    Tissue: Liver.
  4. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
    Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland and Mesenchymal cell.
  6. Lubec G., Klug S.
    Submitted (FEB-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 134-148, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Hippocampus.

Entry informationi

Entry nameiPNPH_MOUSE
AccessioniPrimary (citable) accession number: P23492
Secondary accession number(s): Q4FJT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 1991
Last sequence update: January 31, 1996
Last modified: March 3, 2015
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.