Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P23492 (PNPH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 119. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Purine nucleoside phosphorylase

Short name=PNP
EC=2.4.2.1
Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase
Gene names
Name:Pnp
Synonyms:Np, Pnp1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length289 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Ref.1

Catalytic activity

Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

Pathway

Purine metabolism; purine nucleoside salvage.

Subunit structure

Homotrimer.

Subcellular location

Cytoplasmcytoskeleton By similarity.

Polymorphism

Four electrophoretic alleles of NP are known; NPA (shown here), NPB, NPC and NPD.

Sequence similarities

Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoskeleton
   Coding sequence diversityPolymorphism
   Molecular functionGlycosyltransferase
Transferase
   PTMAcetylation
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processGTP biosynthetic process

Inferred from mutant phenotype PubMed 10859343. Source: MGI

apoptotic mitochondrial changes

Inferred from mutant phenotype PubMed 10859343. Source: MGI

dGTP metabolic process

Inferred from mutant phenotype PubMed 10859343PubMed 9122228. Source: MGI

deoxyguanosine catabolic process

Inferred from mutant phenotype PubMed 10859343. Source: MGI

deoxyinosine catabolic process

Inferred from mutant phenotype PubMed 10859343. Source: MGI

guanosine catabolic process

Inferred from mutant phenotype PubMed 10859343. Source: MGI

inosine catabolic process

Inferred from mutant phenotype PubMed 10859343PubMed 16964310. Source: MGI

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from mutant phenotype PubMed 10859343. Source: MGI

negative regulation of T cell apoptotic process

Inferred from mutant phenotype PubMed 10859343. Source: MGI

positive regulation of B cell differentiation

Inferred from mutant phenotype PubMed 10859343. Source: MGI

positive regulation of DNA repair

Inferred from mutant phenotype PubMed 10859343. Source: MGI

positive regulation of T cell mediated cytotoxicity

Inferred from mutant phenotype PubMed 10859343. Source: MGI

positive regulation of T cell proliferation

Inferred from mutant phenotype PubMed 16964310PubMed 9122228. Source: MGI

positive regulation of alpha-beta T cell differentiation

Inferred from mutant phenotype PubMed 10859343PubMed 16964310PubMed 9122228. Source: MGI

purine nucleoside metabolic process

Inferred from direct assay PubMed 109081Ref.2PubMed 3925939PubMed 405970PubMed 6407470PubMed 7918681PubMed 8663040. Source: MGI

response to gamma radiation

Inferred from mutant phenotype PubMed 10859343. Source: MGI

urate biosynthetic process

Inferred from mutant phenotype PubMed 16964310. Source: MGI

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytosol

Inferred from direct assay PubMed 109081PubMed 3925939PubMed 6407470PubMed 7918681. Source: MGI

   Molecular_functionpurine-nucleoside phosphorylase activity

Inferred from direct assay PubMed 109081PubMed 1235912Ref.2PubMed 1914521PubMed 204065PubMed 3925939PubMed 405970PubMed 6407470PubMed 7323947PubMed 7918681PubMed 8663040. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 289289Purine nucleoside phosphorylase
PRO_0000184537

Amino acid modifications

Modified residue11N-acetylmethionine By similarity

Natural variations

Natural variant221E → K in haplotype NPD.
Natural variant391T → A in haplotype NPD.
Natural variant1521D → E in haplotype NPD.
Natural variant1761T → S in haplotype NPB.
Natural variant2581M → K in haplotype NPC.

Sequences

Sequence LengthMass (Da)Tools
P23492 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: DE7C1C2B004A1113

FASTA28932,277
        10         20         30         40         50         60 
MENEFTYEDY ETTAKWLLQH TEYRPQVAVI CGSGLGGLTA HLKEAQIFDY NEIPNFPQST 

        70         80         90        100        110        120 
VQGHAGRLVF GLLNGRCCVM MQGRFHMYEG YSLSKVTFPV RVFHLLGVET LVVTNAAGGL 

       130        140        150        160        170        180 
NPNFEVGDIM LIRDHINLPG FCGQNPLRGP NDERFGVRFP AMSDAYDRDM RQKAFTAWKQ 

       190        200        210        220        230        240 
MGEQRKLQEG TYVMLAGPNF ETVAESRLLK MLGADAVGMS TVPEVIVARH CGLRVFGFSL 

       250        260        270        280 
ITNKVVMDYE NLEKANHMEV LDAGKAAAQT LERFVSILME SIPLPDRGS 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of murine purine nucleoside phosphorylase cDNA."
Jenuth J.P., Snyder F.F.
Nucleic Acids Res. 19:1708-1708(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]"Isolation and expression of a murine purine nucleoside phosphorylase-encoding cDNA and sequence similarity with the human message."
Nelson D.M., Foresman M.D., Ronnei B.J., McIvor R.S.
Gene 113:215-221(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
[3]"cDNA sequence of four purine nucleoside phosphorylase (Np) alleles in the mouse."
Jenuth J.P., Mangat R.K., Snyder F.F.
Mamm. Genome 4:598-603(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H/HEHA, MOLF/Ei and SPRET-1.
Tissue: Liver.
[4]"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary gland and Mesenchymal cell.
[6]Lubec G., Klug S.
Submitted (MAR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 134-148, IDENTIFICATION BY MASS SPECTROMETRY.
Tissue: Hippocampus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X56548 mRNA. Translation: CAA39888.1.
M84563 mRNA. Translation: AAA39835.1.
L11290 mRNA. Translation: AAC37634.1.
L11291 mRNA. Translation: AAC37635.1.
L11292 mRNA. Translation: AAC37706.1.
U35374 mRNA. Translation: AAB60510.1.
CT010316 mRNA. Translation: CAJ18524.1.
BC003788 mRNA. Translation: AAH03788.1.
BC052679 mRNA. Translation: AAH52679.1.
PIRI57010.
I76672.
RefSeqNP_038660.1. NM_013632.4.
UniGeneMm.17932.

3D structure databases

ProteinModelPortalP23492.
SMRP23492. Positions 3-282.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP23492. 3 interactions.
MINTMINT-1869537.

Chemistry

BindingDBP23492.
ChEMBLCHEMBL2215.

PTM databases

PhosphoSiteP23492.

Proteomic databases

PaxDbP23492.
PRIDEP23492.

Protocols and materials databases

DNASU18950.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID18950.
KEGGmmu:18950.

Organism-specific databases

CTD4860.
MGIMGI:97365. Pnp.

Phylogenomic databases

eggNOGCOG0005.
HOGENOMHOG000045183.
HOVERGENHBG002460.
InParanoidQ4FJT6.
KOK03783.
PhylomeDBP23492.

Enzyme and pathway databases

UniPathwayUPA00606.

Gene expression databases

CleanExMM_PNP1.
GenevestigatorP23492.

Family and domain databases

Gene3D3.40.50.1580. 1 hit.
InterProIPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
IPR011268. Purine_phosphorylase.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERPTHR11904. PTHR11904. 1 hit.
PTHR11904:SF9. PTHR11904:SF9. 1 hit.
PfamPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
PIRSFPIRSF000477. PurNPase. 1 hit.
SUPFAMSSF53167. SSF53167. 1 hit.
TIGRFAMsTIGR01700. PNPH. 1 hit.
TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITEPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295296.
PROP23492.
SOURCESearch...

Entry information

Entry namePNPH_MOUSE
AccessionPrimary (citable) accession number: P23492
Secondary accession number(s): Q4FJT6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot