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P23492

- PNPH_MOUSE

UniProt

P23492 - PNPH_MOUSE

Protein

Purine nucleoside phosphorylase

Gene

Pnp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.1 Publication

    Catalytic activityi

    Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.

    Pathwayi

    GO - Molecular functioni

    1. purine-nucleoside phosphorylase activity Source: MGI

    GO - Biological processi

    1. apoptotic mitochondrial changes Source: MGI
    2. deoxyguanosine catabolic process Source: MGI
    3. deoxyinosine catabolic process Source: MGI
    4. dGTP metabolic process Source: MGI
    5. GTP biosynthetic process Source: MGI
    6. guanosine catabolic process Source: MGI
    7. inosine catabolic process Source: MGI
    8. intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
    9. negative regulation of T cell apoptotic process Source: MGI
    10. positive regulation of alpha-beta T cell differentiation Source: MGI
    11. positive regulation of B cell differentiation Source: MGI
    12. positive regulation of DNA repair Source: MGI
    13. positive regulation of T cell mediated cytotoxicity Source: MGI
    14. positive regulation of T cell proliferation Source: MGI
    15. purine nucleoside metabolic process Source: MGI
    16. response to gamma radiation Source: MGI
    17. urate biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00606.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Purine nucleoside phosphorylase (EC:2.4.2.1)
    Short name:
    PNP
    Alternative name(s):
    Inosine phosphorylase
    Inosine-guanosine phosphorylase
    Gene namesi
    Name:Pnp
    Synonyms:Np, Pnp1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97365. Pnp.

    Subcellular locationi

    Cytoplasmcytoskeleton By similarity

    GO - Cellular componenti

    1. cytoskeleton Source: UniProtKB-SubCell
    2. cytosol Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 289289Purine nucleoside phosphorylasePRO_0000184537Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP23492.
    PaxDbiP23492.
    PRIDEiP23492.

    PTM databases

    PhosphoSiteiP23492.

    Expressioni

    Gene expression databases

    CleanExiMM_PNP1.
    GenevestigatoriP23492.

    Interactioni

    Subunit structurei

    Homotrimer.

    Protein-protein interaction databases

    IntActiP23492. 3 interactions.
    MINTiMINT-1869537.

    Structurei

    3D structure databases

    ProteinModelPortaliP23492.
    SMRiP23492. Positions 3-282.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0005.
    HOGENOMiHOG000045183.
    HOVERGENiHBG002460.
    InParanoidiQ4FJT6.
    KOiK03783.
    PhylomeDBiP23492.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    InterProiIPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
    IPR011268. Purine_phosphorylase.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000477. PurNPase. 1 hit.
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01700. PNPH. 1 hit.
    TIGR01697. PNPH-PUNA-XAPA. 1 hit.
    PROSITEiPS01240. PNP_MTAP_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23492-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENEFTYEDY ETTAKWLLQH TEYRPQVAVI CGSGLGGLTA HLKEAQIFDY    50
    NEIPNFPQST VQGHAGRLVF GLLNGRCCVM MQGRFHMYEG YSLSKVTFPV 100
    RVFHLLGVET LVVTNAAGGL NPNFEVGDIM LIRDHINLPG FCGQNPLRGP 150
    NDERFGVRFP AMSDAYDRDM RQKAFTAWKQ MGEQRKLQEG TYVMLAGPNF 200
    ETVAESRLLK MLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVVMDYE 250
    NLEKANHMEV LDAGKAAAQT LERFVSILME SIPLPDRGS 289
    Length:289
    Mass (Da):32,277
    Last modified:February 1, 1996 - v2
    Checksum:iDE7C1C2B004A1113
    GO

    Polymorphismi

    Four electrophoretic alleles of NP are known; NPA (shown here), NPB, NPC and NPD.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti22 – 221E → K in haplotype NPD.
    Natural varianti39 – 391T → A in haplotype NPD.
    Natural varianti152 – 1521D → E in haplotype NPD.
    Natural varianti176 – 1761T → S in haplotype NPB.
    Natural varianti258 – 2581M → K in haplotype NPC.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56548 mRNA. Translation: CAA39888.1.
    M84563 mRNA. Translation: AAA39835.1.
    L11290 mRNA. Translation: AAC37634.1.
    L11291 mRNA. Translation: AAC37635.1.
    L11292 mRNA. Translation: AAC37706.1.
    U35374 mRNA. Translation: AAB60510.1.
    CT010316 mRNA. Translation: CAJ18524.1.
    BC003788 mRNA. Translation: AAH03788.1.
    BC052679 mRNA. Translation: AAH52679.1.
    CCDSiCCDS27029.1.
    PIRiI57010.
    I76672.
    RefSeqiNP_038660.1. NM_013632.4.
    UniGeneiMm.17932.

    Genome annotation databases

    GeneIDi18950.
    KEGGimmu:18950.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X56548 mRNA. Translation: CAA39888.1 .
    M84563 mRNA. Translation: AAA39835.1 .
    L11290 mRNA. Translation: AAC37634.1 .
    L11291 mRNA. Translation: AAC37635.1 .
    L11292 mRNA. Translation: AAC37706.1 .
    U35374 mRNA. Translation: AAB60510.1 .
    CT010316 mRNA. Translation: CAJ18524.1 .
    BC003788 mRNA. Translation: AAH03788.1 .
    BC052679 mRNA. Translation: AAH52679.1 .
    CCDSi CCDS27029.1.
    PIRi I57010.
    I76672.
    RefSeqi NP_038660.1. NM_013632.4.
    UniGenei Mm.17932.

    3D structure databases

    ProteinModelPortali P23492.
    SMRi P23492. Positions 3-282.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P23492. 3 interactions.
    MINTi MINT-1869537.

    Chemistry

    BindingDBi P23492.
    ChEMBLi CHEMBL2215.

    PTM databases

    PhosphoSitei P23492.

    Proteomic databases

    MaxQBi P23492.
    PaxDbi P23492.
    PRIDEi P23492.

    Protocols and materials databases

    DNASUi 18950.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 18950.
    KEGGi mmu:18950.

    Organism-specific databases

    CTDi 4860.
    MGIi MGI:97365. Pnp.

    Phylogenomic databases

    eggNOGi COG0005.
    HOGENOMi HOG000045183.
    HOVERGENi HBG002460.
    InParanoidi Q4FJT6.
    KOi K03783.
    PhylomeDBi P23492.

    Enzyme and pathway databases

    UniPathwayi UPA00606 .

    Miscellaneous databases

    NextBioi 295296.
    PROi P23492.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PNP1.
    Genevestigatori P23492.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    InterProi IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    IPR011270. Pur_Nuc_Pase_Ino/Guo-sp.
    IPR011268. Purine_phosphorylase.
    IPR018099. Purine_phosphorylase-2_CS.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000477. PurNPase. 1 hit.
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01700. PNPH. 1 hit.
    TIGR01697. PNPH-PUNA-XAPA. 1 hit.
    PROSITEi PS01240. PNP_MTAP_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of murine purine nucleoside phosphorylase cDNA."
      Jenuth J.P., Snyder F.F.
      Nucleic Acids Res. 19:1708-1708(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    2. "Isolation and expression of a murine purine nucleoside phosphorylase-encoding cDNA and sequence similarity with the human message."
      Nelson D.M., Foresman M.D., Ronnei B.J., McIvor R.S.
      Gene 113:215-221(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    3. "cDNA sequence of four purine nucleoside phosphorylase (Np) alleles in the mouse."
      Jenuth J.P., Mangat R.K., Snyder F.F.
      Mamm. Genome 4:598-603(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C3H/HEHA, MOLF/Ei and SPRET-1.
      Tissue: Liver.
    4. "Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)."
      Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland and Mesenchymal cell.
    6. Lubec G., Klug S.
      Submitted (MAR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 134-148, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Hippocampus.

    Entry informationi

    Entry nameiPNPH_MOUSE
    AccessioniPrimary (citable) accession number: P23492
    Secondary accession number(s): Q4FJT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3