P23492 (PNPH_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Purine nucleoside phosphorylase
Short name=PNP
EC=2.4.2.1

Alternative name(s):
Inosine phosphorylase
Inosine-guanosine phosphorylase

Gene names

Name:	Pnp
Synonyms:	Np, Pnp1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	289 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. Ref.1
Catalytic activity	Purine nucleoside + phosphate = purine + alpha-D-ribose 1-phosphate.
Pathway	Purine metabolism; purine nucleoside salvage.
Subunit structure	Homotrimer.
Subcellular location	Cytoplasm › cytoskeleton By similarity.
Polymorphism	Four electrophoretic alleles of NP are known; NPA (shown here), NPB, NPC and NPD.
Sequence similarities	Belongs to the PNP/MTAP phosphorylase family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Coding sequence diversity	Polymorphism
Molecular function	Glycosyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	GTP biosynthetic process Inferred from mutant phenotype PubMed 10859343. Source: MGI dGTP metabolic process Inferred from mutant phenotype PubMed 10859343PubMed 9122228. Source: MGI deoxyguanosine catabolic process Inferred from mutant phenotype PubMed 10859343. Source: MGI deoxyinosine catabolic process Inferred from mutant phenotype PubMed 10859343. Source: MGI guanosine catabolic process Inferred from mutant phenotype PubMed 10859343. Source: MGI inosine catabolic process Inferred from mutant phenotype PubMed 10859343PubMed 16964310. Source: MGI negative regulation of apoptotic process Inferred from mutant phenotype PubMed 10859343. Source: MGI positive regulation of B cell differentiation Inferred from mutant phenotype PubMed 10859343. Source: MGI positive regulation of DNA repair Inferred from mutant phenotype PubMed 10859343. Source: MGI positive regulation of T cell mediated cytotoxicity Inferred from mutant phenotype PubMed 10859343. Source: MGI positive regulation of T cell proliferation Inferred from mutant phenotype PubMed 16964310PubMed 9122228. Source: MGI positive regulation of alpha-beta T cell differentiation Inferred from mutant phenotype PubMed 10859343PubMed 16964310PubMed 9122228. Source: MGI response to gamma radiation Inferred from mutant phenotype PubMed 10859343. Source: MGI urate biosynthetic process Inferred from mutant phenotype PubMed 16964310. Source: MGI
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: UniProtKB-SubCell cytosol Inferred from direct assay PubMed 109081PubMed 3925939PubMed 6407470PubMed 7918681. Source: MGI
Molecular_function	purine-nucleoside phosphorylase activity Inferred from direct assay PubMed 109081PubMed 1235912Ref.2PubMed 1914521PubMed 204065PubMed 3925939PubMed 405970PubMed 6407470PubMed 7323947PubMed 7918681PubMed 8663040. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 289	289	Purine nucleoside phosphorylase		PRO_0000184537
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
Natural variations
	Natural variant	22	1	E → K in haplotype NPD.
	Natural variant	39	1	T → A in haplotype NPD.
	Natural variant	152	1	D → E in haplotype NPD.
	Natural variant	176	1	T → S in haplotype NPB.
	Natural variant	258	1	M → K in haplotype NPC.

Sequences

	Sequence		Length	Mass (Da)
	P23492 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: DE7C1C2B004A1113	FASTA	289	32,277
	10 20 30 40 50 60 MENEFTYEDY ETTAKWLLQH TEYRPQVAVI CGSGLGGLTA HLKEAQIFDY NEIPNFPQST 70 80 90 100 110 120 VQGHAGRLVF GLLNGRCCVM MQGRFHMYEG YSLSKVTFPV RVFHLLGVET LVVTNAAGGL 130 140 150 160 170 180 NPNFEVGDIM LIRDHINLPG FCGQNPLRGP NDERFGVRFP AMSDAYDRDM RQKAFTAWKQ 190 200 210 220 230 240 MGEQRKLQEG TYVMLAGPNF ETVAESRLLK MLGADAVGMS TVPEVIVARH CGLRVFGFSL 250 260 270 280 ITNKVVMDYE NLEKANHMEV LDAGKAAAQT LERFVSILME SIPLPDRGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of murine purine nucleoside phosphorylase cDNA." Jenuth J.P., Snyder F.F. Nucleic Acids Res. 19:1708-1708(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
[2]	"Isolation and expression of a murine purine nucleoside phosphorylase-encoding cDNA and sequence similarity with the human message." Nelson D.M., Foresman M.D., Ronnei B.J., McIvor R.S. Gene 113:215-221(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[3]	"cDNA sequence of four purine nucleoside phosphorylase (Np) alleles in the mouse." Jenuth J.P., Mangat R.K., Snyder F.F. Mamm. Genome 4:598-603(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C3H/HEHA, MOLF/Ei and SPRET-1. Tissue: Liver.
[4]	"Cloning of mouse full open reading frames in Gateway(R) system entry vector (pDONR201)." Ebert L., Muenstermann E., Schatten R., Henze S., Bohn E., Mollenhauer J., Wiemann S., Schick M., Korn B. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland and Mesenchymal cell.
[6]	Lubec G., Klug S. Submitted (MAR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 134-148, MASS SPECTROMETRY. Tissue: Hippocampus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X56548 mRNA. Translation: CAA39888.1. M84563 mRNA. Translation: AAA39835.1. L11290 mRNA. Translation: AAC37634.1. L11291 mRNA. Translation: AAC37635.1. L11292 mRNA. Translation: AAC37706.1. U35374 mRNA. Translation: AAB60510.1. CT010316 mRNA. Translation: CAJ18524.1. BC003788 mRNA. Translation: AAH03788.1. BC052679 mRNA. Translation: AAH52679.1.
IPI	IPI00315452.
PIR	I57010. I76672.
RefSeq	NP_038660.1. NM_013632.4.
UniGene	Mm.17932.
3D structure databases
ProteinModelPortal	P23492.
SMR	P23492. Positions 3-282.
ModBase	Search...
PTM databases
PhosphoSite	P23492.
Proteomic databases
PaxDb	P23492.
PRIDE	P23492.
Protocols and materials databases
DNASU	18950.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	18950.
KEGG	mmu:18950.
Organism-specific databases
CTD	4860.
MGI	MGI:97365. Pnp.
Phylogenomic databases
eggNOG	COG0005.
HOGENOM	HOG000045183.
HOVERGEN	HBG002460.
InParanoid	Q4FJT6.
KO	K03783.
OrthoDB	EOG4CZBGH.
Enzyme and pathway databases
UniPathway	UPA00606.
Gene expression databases
CleanEx	MM_PNP1.
Genevestigator	P23492.
GermOnline	ENSMUSG00000021871. Mus musculus.
Family and domain databases
InterPro	IPR000845. Nucleoside_phosphorylase_d. IPR001369. PNP/MTAP. IPR011270. Pur_Nuc_Pase_Ino/Guo-sp. IPR011268. Purine_phosphorylase. IPR018099. Purine_phosphorylase-2_CS. [Graphical view]
PANTHER	PTHR11904. PTHR11904. 1 hit. PTHR11904:SF9. PTHR11904:SF9. 1 hit.
Pfam	PF01048. PNP_UDP_1. 1 hit. [Graphical view]
PIRSF	PIRSF000477. PurNPase. 1 hit.
TIGRFAMs	TIGR01700. PNPH. 1 hit. TIGR01697. PNPH-PUNA-XAPA. 1 hit.
PROSITE	PS01240. PNP_MTAP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P23492.
ChEMBL	CHEMBL2215.
NextBio	295296.
SOURCE	Search...

Entry information

Entry name

PNPH_MOUSE

Accession

Primary (citable) accession number: P23492
Secondary accession number(s): Q4FJT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 111 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program