Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P23491 (ZP3_MESAU)

Last modified October 13, 2009. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Zona pellucida sperm-binding protein 3
Alternative name(s):
    Zona pellucida glycoprotein ZP3
    Zona pellucida protein C
    Sperm receptor
Cleaved into the following chain:
    1- Recommended name:
            Processed zona pellucida sperm-binding protein 3
Gene names
Name: ZP3
Synonyms: ZPC
OrganismMesocricetus auratus (Golden hamster)
Taxonomic identifier10036 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Mesocricetus

Hide | Top

Protein attributes

Sequence length422 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

Hide | Top

General annotation (Comments)

Function

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

Subunit structure

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers By similarity.

Subcellular location

Processed zona pellucida sperm-binding protein 3: Secreted › extracellular space › extracellular matrix.

Cell membrane; Single-pass type I membrane protein.

Tissue specificity

Oocytes.

Developmental stage

Growing oocytes.

Domain

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Post-translational modification

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.

N-glycosylated By similarity.

O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy By similarity.

Sequence similarities

Belongs to the ZP domain family. ZPC subfamily.

Contains 1 ZP domain.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 By similarity
Chain23 – 349327Zona pellucida sperm-binding protein 3
PRO_0000041713
Chain23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304571
Propeptide350 – 42273Removed in mature form By similarity
PRO_0000041714

Regions

Topological domain23 – 386364Extracellular Potential
Transmembrane387 – 40721 Potential
Topological domain408 – 42215Cytoplasmic Potential
Domain45 – 306262ZP
Compositional bias119 – 15840Pro-rich
Compositional bias208 – 25750Pro-rich

Amino acid modifications

Modified residue231Pyrrolidone carboxylic acid By similarity
Glycosylation321O-linked (GalNAc...) By similarity
Glycosylation341O-linked (GalNAc...) By similarity
Glycosylation1461N-linked (GlcNAc...) By similarity
Glycosylation1551O-linked (GalNAc...) By similarity
Glycosylation1611O-linked (GalNAc...) By similarity
Glycosylation1621O-linked (GalNAc...) By similarity
Glycosylation2711N-linked (GlcNAc...) By similarity
Glycosylation3021N-linked (GlcNAc...) By similarity
Disulfide bond46 ↔ 139 By similarity
Disulfide bond78 ↔ 98 By similarity
Disulfide bond216 ↔ 281 By similarity
Disulfide bond238 ↔ 299 By similarity

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P23491-1 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: D0F95BE7FF8E7E01

FASTA42245,827
        10         20         30         40         50         60 
MGLSYQLLLC LLLCGGAKQC CSQPLWLLPG GTPTPGKLTS SVEVECLEAE LVVTVSRDLF 

        70         80         90        100        110        120 
GTGKLIQPED LTLGSENCRP LVSVATDVVR FKAQLHECSN RVQVTEDALV YSTVLLHQPR 

       130        140        150        160        170        180 
PVPGLSILRT NRADVPIECR YPRQGNVSSH AIRPTWVPFS TTVSSEEKLV FSLRLMEENW 

       190        200        210        220        230        240 
NTEKLSPTSH LGEVAYLQAE VQTGSHLPLL LFVDRCVPTP SPDQTASPYH VIVDFHGCLV 

       250        260        270        280        290        300 
DGLSESFSAF QVPRPRPETL QFTVDVFHFA NSSRNTIYIT CHLKVTPANQ TPDELNKACS 

       310        320        330        340        350        360 
FNRSSKSWSP VEGDAEVCGC CSSGDCGSSS RSRYQAHGVS QWPKSASRRR RHVRDEADVT 

       370        380        390        400        410        420 
VGPLIFLGKA SDQAVEGWAS SAQTSLALGL GLAAVAFLTL AAIVLGVTRS CHTPSHVVSL 


SQ

« Hide

Hide | Top

References

[1]"Genomic organization and polypeptide primary structure of zona pellucida glycoprotein hZP3, the hamster sperm receptor."
Kinloch R.A., Ruiz-Seller B., Wassarman P.M.
Dev. Biol. 142:414-421(1990) [PubMed: 2257975] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.

Hide | Top

Web resources

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Hide | Top

Cross-references

Sequence databases

M63629 mRNA. Translation: AAA37079.1.
PIRA60503.

3D structure databases

ModBaseSearch...

Phylogenomic databases

HOVERGENP23491.

Family and domain databases

InterProIPR001507. Endoglin/CD105.
IPR017977. Endoglin/CD105_CS.
IPR017976. Endoglin/CD105_subgr.
[Graphical view]
PfamPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSPR00023. ZPELLUCIDA.
SMARTSM00241. ZP. 1 hit.
[Graphical view]
PROSITEPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Hide | Top

Entry information

Entry nameZP3_MESAU
AccessionPrimary (citable) accession number: P23491
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: October 13, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

Protein Spotlight

Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Web resources · Cross-references · Entry information · Relevant documents