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P23491

- ZP3_MESAU

UniProt

P23491 - ZP3_MESAU

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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Mesocricetus auratus (Golden hamster)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for sperm binding and zona matrix formation.

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB
  2. manganese ion transmembrane transporter activity Source: UniProtKB
  3. signal transducer activity Source: UniProtKB

GO - Biological processi

  1. binding of sperm to zona pellucida Source: UniProtKB
  2. blastocyst formation Source: UniProtKB
  3. egg coat formation Source: UniProtKB
  4. humoral immune response mediated by circulating immunoglobulin Source: UniProtKB
  5. intracellular protein transport Source: UniProtKB
  6. intracellular signal transduction Source: UniProtKB
  7. manganese ion transmembrane transport Source: GOC
  8. manganese ion transport Source: UniProtKB
  9. negative regulation of binding of sperm to zona pellucida Source: UniProtKB
  10. negative regulation of transcription, DNA-templated Source: UniProtKB
  11. oocyte development Source: UniProtKB
  12. phosphatidylinositol-mediated signaling Source: UniProtKB
  13. positive regulation of acrosomal vesicle exocytosis Source: UniProtKB
  14. positive regulation of acrosome reaction Source: UniProtKB
  15. positive regulation of antral ovarian follicle growth Source: UniProtKB
  16. positive regulation of calcium ion import Source: UniProtKB
  17. positive regulation of humoral immune response Source: UniProtKB
  18. positive regulation of inflammatory response Source: UniProtKB
  19. positive regulation of interferon-gamma production Source: UniProtKB
  20. positive regulation of interleukin-4 production Source: UniProtKB
  21. positive regulation of leukocyte migration Source: UniProtKB
  22. positive regulation of ovarian follicle development Source: UniProtKB
  23. positive regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  24. positive regulation of protein kinase activity Source: UniProtKB
  25. positive regulation of protein kinase B signaling Source: UniProtKB
  26. positive regulation of T cell proliferation Source: UniProtKB
  27. positive regulation of transcription, DNA-templated Source: UniProtKB
  28. positive regulation of type IV hypersensitivity Source: UniProtKB
  29. protein kinase C signaling Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Fertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZPC
OrganismiMesocricetus auratus (Golden hamster)
Taxonomic identifieri10036 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeMesocricetus

Subcellular locationi

Chain Processed zona pellucida sperm-binding protein 3 : Secretedextracellular spaceextracellular matrix
Note: The glycoproteinaceous translucent extracellular matrix that surrounds the mammalian oocyte is called zona pellucida.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB
  3. extracellular matrix Source: UniProtKB
  4. extracellular space Source: UniProtKB
  5. Golgi apparatus Source: UniProtKB
  6. integral component of membrane Source: UniProtKB-KW
  7. multivesicular body Source: UniProtKB
  8. outer acrosomal membrane Source: UniProtKB
  9. perinuclear region of cytoplasm Source: UniProtKB
  10. plasma membrane Source: UniProtKB
  11. proteinaceous extracellular matrix Source: UniProtKB-KW
  12. secretory granule Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222By similarityAdd
BLAST
Chaini23 – 349327Zona pellucida sperm-binding protein 3PRO_0000041713Add
BLAST
Chaini23 – ?Processed zona pellucida sperm-binding protein 3PRO_0000304571
Propeptidei350 – 42273Removed in mature formBy similarityPRO_0000041714Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei23 – 231Pyrrolidone carboxylic acidBy similarity
Glycosylationi32 – 321O-linked (GalNAc...)By similarity
Glycosylationi34 – 341O-linked (GalNAc...)By similarity
Disulfide bondi46 ↔ 139By similarity
Disulfide bondi78 ↔ 98By similarity
Glycosylationi146 – 1461N-linked (GlcNAc...)By similarity
Glycosylationi155 – 1551O-linked (GalNAc...)By similarity
Glycosylationi161 – 1611O-linked (GalNAc...)By similarity
Glycosylationi162 – 1621O-linked (GalNAc...)By similarity
Disulfide bondi216 ↔ 281By similarity
Disulfide bondi238 ↔ 299By similarity
Glycosylationi271 – 2711N-linked (GlcNAc...)By similarity
Glycosylationi302 – 3021N-linked (GlcNAc...)By similarity

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.
N-glycosylated.By similarity
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Oocytes.

Developmental stagei

Growing oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers.By similarity

Structurei

3D structure databases

ProteinModelPortaliP23491.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 386364ExtracellularSequence AnalysisAdd
BLAST
Topological domaini408 – 42215CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei387 – 40721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 306262ZPPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi119 – 15840Pro-richAdd
BLAST
Compositional biasi208 – 25750Pro-richAdd
BLAST

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated
Contains 1 ZP domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG007985.

Family and domain databases

InterProiIPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view]
PfamiPF00100. Zona_pellucida. 1 hit.
[Graphical view]
PRINTSiPR00023. ZPELLUCIDA.
SMARTiSM00241. ZP. 1 hit.
[Graphical view]
PROSITEiPS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23491-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLSYQLLLC LLLCGGAKQC CSQPLWLLPG GTPTPGKLTS SVEVECLEAE
60 70 80 90 100
LVVTVSRDLF GTGKLIQPED LTLGSENCRP LVSVATDVVR FKAQLHECSN
110 120 130 140 150
RVQVTEDALV YSTVLLHQPR PVPGLSILRT NRADVPIECR YPRQGNVSSH
160 170 180 190 200
AIRPTWVPFS TTVSSEEKLV FSLRLMEENW NTEKLSPTSH LGEVAYLQAE
210 220 230 240 250
VQTGSHLPLL LFVDRCVPTP SPDQTASPYH VIVDFHGCLV DGLSESFSAF
260 270 280 290 300
QVPRPRPETL QFTVDVFHFA NSSRNTIYIT CHLKVTPANQ TPDELNKACS
310 320 330 340 350
FNRSSKSWSP VEGDAEVCGC CSSGDCGSSS RSRYQAHGVS QWPKSASRRR
360 370 380 390 400
RHVRDEADVT VGPLIFLGKA SDQAVEGWAS SAQTSLALGL GLAAVAFLTL
410 420
AAIVLGVTRS CHTPSHVVSL SQ
Length:422
Mass (Da):45,827
Last modified:February 1, 1996 - v2
Checksum:iD0F95BE7FF8E7E01
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63629 mRNA. Translation: AAA37079.1.
PIRiA60503.
RefSeqiNP_001268531.1. NM_001281602.1.

Genome annotation databases

GeneIDi101824371.

Cross-referencesi

Web resourcesi

Protein Spotlight

Molecular chastity - Issue 93 of April 2008

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63629 mRNA. Translation: AAA37079.1 .
PIRi A60503.
RefSeqi NP_001268531.1. NM_001281602.1.

3D structure databases

ProteinModelPortali P23491.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 101824371.

Organism-specific databases

CTDi 7784.

Phylogenomic databases

HOVERGENi HBG007985.

Family and domain databases

InterProi IPR001507. ZP_dom.
IPR017977. ZP_dom_CS.
[Graphical view ]
Pfami PF00100. Zona_pellucida. 1 hit.
[Graphical view ]
PRINTSi PR00023. ZPELLUCIDA.
SMARTi SM00241. ZP. 1 hit.
[Graphical view ]
PROSITEi PS00682. ZP_1. 1 hit.
PS51034. ZP_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genomic organization and polypeptide primary structure of zona pellucida glycoprotein hZP3, the hamster sperm receptor."
    Kinloch R.A., Ruiz-Seller B., Wassarman P.M.
    Dev. Biol. 142:414-421(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.

Entry informationi

Entry nameiZP3_MESAU
AccessioniPrimary (citable) accession number: P23491
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 77 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3