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Protein

Hydrogenase-4 component B

Gene

hyfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-153.
ECOL316407:JW2467-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component B (EC:1.-.-.-)
Gene namesi
Name:hyfB
Synonyms:yffF
Ordered Locus Names:b2482, JW2467
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11282. hyfB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55PeriplasmicSequence analysis
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Topological domaini27 – 304CytoplasmicSequence analysis
Transmembranei31 – 5121HelicalSequence analysisAdd
BLAST
Topological domaini52 – 7928PeriplasmicSequence analysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence analysisAdd
BLAST
Topological domaini101 – 11919CytoplasmicSequence analysisAdd
BLAST
Transmembranei120 – 14021HelicalSequence analysisAdd
BLAST
Topological domaini141 – 16424PeriplasmicSequence analysisAdd
BLAST
Transmembranei165 – 18521HelicalSequence analysisAdd
BLAST
Topological domaini186 – 19914CytoplasmicSequence analysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence analysisAdd
BLAST
Topological domaini221 – 24222PeriplasmicSequence analysisAdd
BLAST
Transmembranei243 – 26321HelicalSequence analysisAdd
BLAST
Topological domaini264 – 2729CytoplasmicSequence analysis
Transmembranei273 – 29321HelicalSequence analysisAdd
BLAST
Topological domaini294 – 31118PeriplasmicSequence analysisAdd
BLAST
Transmembranei312 – 33221HelicalSequence analysisAdd
BLAST
Topological domaini333 – 34210CytoplasmicSequence analysis
Transmembranei343 – 36321HelicalSequence analysisAdd
BLAST
Topological domaini364 – 38421PeriplasmicSequence analysisAdd
BLAST
Transmembranei385 – 40521HelicalSequence analysisAdd
BLAST
Topological domaini406 – 42722CytoplasmicSequence analysisAdd
BLAST
Transmembranei428 – 44821HelicalSequence analysisAdd
BLAST
Topological domaini449 – 47426PeriplasmicSequence analysisAdd
BLAST
Transmembranei475 – 49521HelicalSequence analysisAdd
BLAST
Topological domaini496 – 5049CytoplasmicSequence analysis
Transmembranei505 – 52521HelicalSequence analysisAdd
BLAST
Topological domaini526 – 5316PeriplasmicSequence analysis
Transmembranei532 – 55221HelicalSequence analysisAdd
BLAST
Topological domaini553 – 65199CytoplasmicSequence analysisAdd
BLAST
Transmembranei652 – 67221HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 672672Hydrogenase-4 component BPRO_0000118226Add
BLAST

Proteomic databases

PaxDbiP23482.

Interactioni

Protein-protein interaction databases

BioGridi4259196. 5 interactions.
DIPiDIP-9984N.
IntActiP23482. 3 interactions.
MINTiMINT-1254904.
STRINGi511145.b2482.

Structurei

3D structure databases

ProteinModelPortaliP23482.
SMRiP23482. Positions 73-436.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JPZ. Bacteria.
COG0651. LUCA.
HOGENOMiHOG000278686.
InParanoidiP23482.
KOiK12137.
OMAiAAMCFVK.
OrthoDBiEOG6CCH3Q.
PhylomeDBiP23482.

Family and domain databases

InterProiIPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.

Sequencei

Sequence statusi: Complete.

P23482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALQLLTWS LILYLFASLA SLFLLGLDRL AIKLSGITSL VGGVIGIISG
60 70 80 90 100
ITQLHAGVTL VARFAPPFEF ADLTLRMDSL SAFMVLVISL LVVVCSLYSL
110 120 130 140 150
TYMREYEGKG AAAMGFFMNI FIASMVALLV MDNAFWFIVL FEMMSLSSWF
160 170 180 190 200
LVIARQDKTS INAGMLYFFI AHAGSVLIMI AFLLMGRESG SLDFASFRTL
210 220 230 240 250
SLSPGLASAV FLLAFFGFGA KAGMMPLHSW LPRAHPAAPS HASALMSGVM
260 270 280 290 300
VKIGIFGILK VAMDLLAQTG LPLWWGILVM AIGAISALLG VLYALAEQDI
310 320 330 340 350
KRLLAWSTVE NVGIILLAVG VAMVGLSLHD PLLTVVGLLG ALFHLLNHAL
360 370 380 390 400
FKGLLFLGAG AIISRLHTHD MEKMGALAKR MPWTAAACLI GCLAISAIPP
410 420 430 440 450
LNGFISEWYT WQSLFSLSRV EAVALQLAGP IAMVMLAVTG GLAVMCFVKM
460 470 480 490 500
YGITFCGAPR STHAEEAQEV PNTMIVAMLL LAALCVLIAL SASWLAPKIM
510 520 530 540 550
HIAHAFTNTP PATVASGIAL VPGTFHTQVT PSLLLLLLLA MPLLPGLYWL
560 570 580 590 600
WCRSRRAAFR RTGDAWACGY GWENAMAPSG NGVMQPLRVV FSALFRLRQQ
610 620 630 640 650
LDPTLRLNKG LAHVTARAQS TEPFWDERVI RPIVSATQRL AKEIQHLQSG
660 670
DFRLYCLYVV AALVVLLIAI AV
Length:672
Mass (Da):72,583
Last modified:November 1, 1997 - v2
Checksum:i3CA8C2BD25E54B1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88564.1.
U00096 Genomic DNA. Translation: AAC75535.1.
AP009048 Genomic DNA. Translation: BAA16360.1.
PIRiA65024.
RefSeqiNP_416977.1. NC_000913.3.
WP_000339455.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75535; AAC75535; b2482.
BAA16360; BAA16360; BAA16360.
GeneIDi946961.
KEGGiecj:JW2467.
eco:b2482.
PATRICi32120351. VBIEscCol129921_2578.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88564.1.
U00096 Genomic DNA. Translation: AAC75535.1.
AP009048 Genomic DNA. Translation: BAA16360.1.
PIRiA65024.
RefSeqiNP_416977.1. NC_000913.3.
WP_000339455.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23482.
SMRiP23482. Positions 73-436.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259196. 5 interactions.
DIPiDIP-9984N.
IntActiP23482. 3 interactions.
MINTiMINT-1254904.
STRINGi511145.b2482.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP23482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75535; AAC75535; b2482.
BAA16360; BAA16360; BAA16360.
GeneIDi946961.
KEGGiecj:JW2467.
eco:b2482.
PATRICi32120351. VBIEscCol129921_2578.

Organism-specific databases

EchoBASEiEB1259.
EcoGeneiEG11282. hyfB.

Phylogenomic databases

eggNOGiENOG4108JPZ. Bacteria.
COG0651. LUCA.
HOGENOMiHOG000278686.
InParanoidiP23482.
KOiK12137.
OMAiAAMCFVK.
OrthoDBiEOG6CCH3Q.
PhylomeDBiP23482.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-153.
ECOL316407:JW2467-MONOMER.

Miscellaneous databases

PROiP23482.

Family and domain databases

InterProiIPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Andrews S.C., McClay J., Ambler A., Quail M., Berks B.C., Guest J.R.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map."
    Andrews S.C., Harrison P.M., Guest J.R.
    J. Gen. Microbiol. 137:361-367(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.
    Strain: K12.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiHYFB_ECOLI
AccessioniPrimary (citable) accession number: P23482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: January 20, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.