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Protein

Hydrogenase-4 component B

Gene

hyfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Possible component of hydrogenase 4.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-153

Protein family/group databases

TCDBi3.D.1.9.1 the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component B (EC:1.-.-.-)
Gene namesi
Name:hyfB1 Publication
Synonyms:yffF
Ordered Locus Names:b2482, JW2467
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11282 hyfB

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5PeriplasmicSequence analysis5
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Topological domaini27 – 30CytoplasmicSequence analysis4
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 79PeriplasmicSequence analysisAdd BLAST28
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 119CytoplasmicSequence analysisAdd BLAST19
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 164PeriplasmicSequence analysisAdd BLAST24
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 199CytoplasmicSequence analysisAdd BLAST14
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 242PeriplasmicSequence analysisAdd BLAST22
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 272CytoplasmicSequence analysis9
Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
Topological domaini294 – 311PeriplasmicSequence analysisAdd BLAST18
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 342CytoplasmicSequence analysis10
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Topological domaini364 – 384PeriplasmicSequence analysisAdd BLAST21
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 427CytoplasmicSequence analysisAdd BLAST22
Transmembranei428 – 448HelicalSequence analysisAdd BLAST21
Topological domaini449 – 474PeriplasmicSequence analysisAdd BLAST26
Transmembranei475 – 495HelicalSequence analysisAdd BLAST21
Topological domaini496 – 504CytoplasmicSequence analysis9
Transmembranei505 – 525HelicalSequence analysisAdd BLAST21
Topological domaini526 – 531PeriplasmicSequence analysis6
Transmembranei532 – 552HelicalSequence analysisAdd BLAST21
Topological domaini553 – 651CytoplasmicSequence analysisAdd BLAST99
Transmembranei652 – 672HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of most of the operon (hyfBCDEFGHIJR) has no visible effect under fermentative growth conditions at pH 6.5 or 7.5, during aerobic or anaerobic glucose-limited growth at pH 6.5, or when combined with hyc mutations.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001182261 – 672Hydrogenase-4 component BAdd BLAST672

Proteomic databases

PaxDbiP23482
PRIDEiP23482

Expressioni

Inductioni

Most efficiently induced by formate during post-exponential growth at low external pH (pH 6.5) in the absence of respiratory electron acceptors O2+, NO3- or trimethylamine-N-oxide, i.e. under anaerobic control. Transcription is activated by FhlA and HyfR, inhibited by HycA, part of the sigma-54 (rpoN) regulon. Second member of a 10 gene operon (hyfABCDEFGHIJ); it is not clear if the 2 following genes (hydR-focB) are also in the operon.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
ychJP370522EBI-554793,EBI-560596

Protein-protein interaction databases

BioGridi4259196, 9 interactors
DIPiDIP-9984N
IntActiP23482, 3 interactors
STRINGi316385.ECDH10B_2648

Structurei

3D structure databases

ProteinModelPortaliP23482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JPZ Bacteria
COG0651 LUCA
HOGENOMiHOG000278686
InParanoidiP23482
KOiK12137
OMAiAMCFVKV
PhylomeDBiP23482

Family and domain databases

InterProiView protein in InterPro
IPR003918 NADH_UbQ_OxRdtase
IPR001750 ND/Mrp_mem
PfamiView protein in Pfam
PF00361 Proton_antipo_M, 1 hit
PRINTSiPR01437 NUOXDRDTASE4

Sequencei

Sequence statusi: Complete.

P23482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALQLLTWS LILYLFASLA SLFLLGLDRL AIKLSGITSL VGGVIGIISG
60 70 80 90 100
ITQLHAGVTL VARFAPPFEF ADLTLRMDSL SAFMVLVISL LVVVCSLYSL
110 120 130 140 150
TYMREYEGKG AAAMGFFMNI FIASMVALLV MDNAFWFIVL FEMMSLSSWF
160 170 180 190 200
LVIARQDKTS INAGMLYFFI AHAGSVLIMI AFLLMGRESG SLDFASFRTL
210 220 230 240 250
SLSPGLASAV FLLAFFGFGA KAGMMPLHSW LPRAHPAAPS HASALMSGVM
260 270 280 290 300
VKIGIFGILK VAMDLLAQTG LPLWWGILVM AIGAISALLG VLYALAEQDI
310 320 330 340 350
KRLLAWSTVE NVGIILLAVG VAMVGLSLHD PLLTVVGLLG ALFHLLNHAL
360 370 380 390 400
FKGLLFLGAG AIISRLHTHD MEKMGALAKR MPWTAAACLI GCLAISAIPP
410 420 430 440 450
LNGFISEWYT WQSLFSLSRV EAVALQLAGP IAMVMLAVTG GLAVMCFVKM
460 470 480 490 500
YGITFCGAPR STHAEEAQEV PNTMIVAMLL LAALCVLIAL SASWLAPKIM
510 520 530 540 550
HIAHAFTNTP PATVASGIAL VPGTFHTQVT PSLLLLLLLA MPLLPGLYWL
560 570 580 590 600
WCRSRRAAFR RTGDAWACGY GWENAMAPSG NGVMQPLRVV FSALFRLRQQ
610 620 630 640 650
LDPTLRLNKG LAHVTARAQS TEPFWDERVI RPIVSATQRL AKEIQHLQSG
660 670
DFRLYCLYVV AALVVLLIAI AV
Length:672
Mass (Da):72,583
Last modified:November 1, 1997 - v2
Checksum:i3CA8C2BD25E54B1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA Translation: AAB88564.1
U00096 Genomic DNA Translation: AAC75535.1
AP009048 Genomic DNA Translation: BAA16360.1
PIRiA65024
RefSeqiNP_416977.1, NC_000913.3
WP_000339455.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC75535; AAC75535; b2482
BAA16360; BAA16360; BAA16360
GeneIDi946961
KEGGiecj:JW2467
eco:b2482
PATRICifig|1411691.4.peg.4257

Similar proteinsi

Entry informationi

Entry nameiHYFB_ECOLI
AccessioniPrimary (citable) accession number: P23482
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health