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Protein

Hydrogenase-4 component B

Gene

hyfB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-153.
ECOL316407:JW2467-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component B (EC:1.-.-.-)
Gene namesi
Name:hyfB
Synonyms:yffF
Ordered Locus Names:b2482, JW2467
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11282. hyfB.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 5PeriplasmicSequence analysis5
Transmembranei6 – 26HelicalSequence analysisAdd BLAST21
Topological domaini27 – 30CytoplasmicSequence analysis4
Transmembranei31 – 51HelicalSequence analysisAdd BLAST21
Topological domaini52 – 79PeriplasmicSequence analysisAdd BLAST28
Transmembranei80 – 100HelicalSequence analysisAdd BLAST21
Topological domaini101 – 119CytoplasmicSequence analysisAdd BLAST19
Transmembranei120 – 140HelicalSequence analysisAdd BLAST21
Topological domaini141 – 164PeriplasmicSequence analysisAdd BLAST24
Transmembranei165 – 185HelicalSequence analysisAdd BLAST21
Topological domaini186 – 199CytoplasmicSequence analysisAdd BLAST14
Transmembranei200 – 220HelicalSequence analysisAdd BLAST21
Topological domaini221 – 242PeriplasmicSequence analysisAdd BLAST22
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 272CytoplasmicSequence analysis9
Transmembranei273 – 293HelicalSequence analysisAdd BLAST21
Topological domaini294 – 311PeriplasmicSequence analysisAdd BLAST18
Transmembranei312 – 332HelicalSequence analysisAdd BLAST21
Topological domaini333 – 342CytoplasmicSequence analysis10
Transmembranei343 – 363HelicalSequence analysisAdd BLAST21
Topological domaini364 – 384PeriplasmicSequence analysisAdd BLAST21
Transmembranei385 – 405HelicalSequence analysisAdd BLAST21
Topological domaini406 – 427CytoplasmicSequence analysisAdd BLAST22
Transmembranei428 – 448HelicalSequence analysisAdd BLAST21
Topological domaini449 – 474PeriplasmicSequence analysisAdd BLAST26
Transmembranei475 – 495HelicalSequence analysisAdd BLAST21
Topological domaini496 – 504CytoplasmicSequence analysis9
Transmembranei505 – 525HelicalSequence analysisAdd BLAST21
Topological domaini526 – 531PeriplasmicSequence analysis6
Transmembranei532 – 552HelicalSequence analysisAdd BLAST21
Topological domaini553 – 651CytoplasmicSequence analysisAdd BLAST99
Transmembranei652 – 672HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001182261 – 672Hydrogenase-4 component BAdd BLAST672

Proteomic databases

PaxDbiP23482.
PRIDEiP23482.

Interactioni

Protein-protein interaction databases

BioGridi4259196. 5 interactors.
DIPiDIP-9984N.
IntActiP23482. 3 interactors.
MINTiMINT-1254904.
STRINGi511145.b2482.

Structurei

3D structure databases

ProteinModelPortaliP23482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 5 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108JPZ. Bacteria.
COG0651. LUCA.
HOGENOMiHOG000278686.
InParanoidiP23482.
KOiK12137.
OMAiAAMCFVK.
PhylomeDBiP23482.

Family and domain databases

InterProiIPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.

Sequencei

Sequence statusi: Complete.

P23482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDALQLLTWS LILYLFASLA SLFLLGLDRL AIKLSGITSL VGGVIGIISG
60 70 80 90 100
ITQLHAGVTL VARFAPPFEF ADLTLRMDSL SAFMVLVISL LVVVCSLYSL
110 120 130 140 150
TYMREYEGKG AAAMGFFMNI FIASMVALLV MDNAFWFIVL FEMMSLSSWF
160 170 180 190 200
LVIARQDKTS INAGMLYFFI AHAGSVLIMI AFLLMGRESG SLDFASFRTL
210 220 230 240 250
SLSPGLASAV FLLAFFGFGA KAGMMPLHSW LPRAHPAAPS HASALMSGVM
260 270 280 290 300
VKIGIFGILK VAMDLLAQTG LPLWWGILVM AIGAISALLG VLYALAEQDI
310 320 330 340 350
KRLLAWSTVE NVGIILLAVG VAMVGLSLHD PLLTVVGLLG ALFHLLNHAL
360 370 380 390 400
FKGLLFLGAG AIISRLHTHD MEKMGALAKR MPWTAAACLI GCLAISAIPP
410 420 430 440 450
LNGFISEWYT WQSLFSLSRV EAVALQLAGP IAMVMLAVTG GLAVMCFVKM
460 470 480 490 500
YGITFCGAPR STHAEEAQEV PNTMIVAMLL LAALCVLIAL SASWLAPKIM
510 520 530 540 550
HIAHAFTNTP PATVASGIAL VPGTFHTQVT PSLLLLLLLA MPLLPGLYWL
560 570 580 590 600
WCRSRRAAFR RTGDAWACGY GWENAMAPSG NGVMQPLRVV FSALFRLRQQ
610 620 630 640 650
LDPTLRLNKG LAHVTARAQS TEPFWDERVI RPIVSATQRL AKEIQHLQSG
660 670
DFRLYCLYVV AALVVLLIAI AV
Length:672
Mass (Da):72,583
Last modified:November 1, 1997 - v2
Checksum:i3CA8C2BD25E54B1B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88564.1.
U00096 Genomic DNA. Translation: AAC75535.1.
AP009048 Genomic DNA. Translation: BAA16360.1.
PIRiA65024.
RefSeqiNP_416977.1. NC_000913.3.
WP_000339455.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75535; AAC75535; b2482.
BAA16360; BAA16360; BAA16360.
GeneIDi946961.
KEGGiecj:JW2467.
eco:b2482.
PATRICi32120351. VBIEscCol129921_2578.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88564.1.
U00096 Genomic DNA. Translation: AAC75535.1.
AP009048 Genomic DNA. Translation: BAA16360.1.
PIRiA65024.
RefSeqiNP_416977.1. NC_000913.3.
WP_000339455.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259196. 5 interactors.
DIPiDIP-9984N.
IntActiP23482. 3 interactors.
MINTiMINT-1254904.
STRINGi511145.b2482.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP23482.
PRIDEiP23482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75535; AAC75535; b2482.
BAA16360; BAA16360; BAA16360.
GeneIDi946961.
KEGGiecj:JW2467.
eco:b2482.
PATRICi32120351. VBIEscCol129921_2578.

Organism-specific databases

EchoBASEiEB1259.
EcoGeneiEG11282. hyfB.

Phylogenomic databases

eggNOGiENOG4108JPZ. Bacteria.
COG0651. LUCA.
HOGENOMiHOG000278686.
InParanoidiP23482.
KOiK12137.
OMAiAAMCFVK.
PhylomeDBiP23482.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-153.
ECOL316407:JW2467-MONOMER.

Miscellaneous databases

PROiP23482.

Family and domain databases

InterProiIPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
ProtoNetiSearch...

Entry informationi

Entry nameiHYFB_ECOLI
AccessioniPrimary (citable) accession number: P23482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1997
Last modified: November 2, 2016
This is version 127 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.