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Protein

Hydrogenase-4 component A

Gene

hyfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Probable electron transfer protein for hydrogenase 4.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi12 – 121Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi15 – 151Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi18 – 181Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi22 – 221Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi51 – 511Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi54 – 541Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi59 – 591Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi63 – 631Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi82 – 821Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi85 – 851Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi88 – 881Iron-sulfur 4 (4Fe-4S)By similarity
Metal bindingi92 – 921Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi146 – 1461Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi149 – 1491Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi158 – 1581Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi162 – 1621Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-152.
ECOL316407:JW2466-MONOMER.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component A (EC:1.-.-.-)
Gene namesi
Name:hyfA
Synonyms:yffE
Ordered Locus Names:b2481, JW2466
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11150. hyfA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 205205Hydrogenase-4 component APRO_0000159267Add
BLAST

Proteomic databases

PaxDbiP23481.
PRIDEiP23481.

Interactioni

Protein-protein interaction databases

BioGridi4259199. 13 interactions.
DIPiDIP-9983N.
IntActiP23481. 3 interactions.
STRINGi511145.b2481.

Structurei

3D structure databases

ProteinModelPortaliP23481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 31304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini41 – 72324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini73 – 102304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST
Domaini140 – 172334Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 4 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP23481.
KOiK12136.
OMAiKLCGLVC.
OrthoDBiEOG6XSZQF.
PhylomeDBiP23481.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF12798. Fer4_3. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFVVAEPL WCTGCNTCLA ACSDVHKTQG LQQHPRLALA KTSTITAPVV
60 70 80 90 100
CHHCEEAPCL QVCPVNAISQ RDDAIQLNES LCIGCKLCAV VCPFGAISAS
110 120 130 140 150
GSRPVNAHAQ YVFQAEGSLK DGEENAPTQH ALLRWEPGVQ TVAVKCDLCD
160 170 180 190 200
FLPEGPACVR ACPNQALRLI TGDSLQRQMK EKQRLAASWF ANGGEDPLSL

TQEQR
Length:205
Mass (Da):22,154
Last modified:November 1, 1991 - v1
Checksum:iC06ED8FB0617B46B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88563.1.
U00096 Genomic DNA. Translation: AAC75534.2.
AP009048 Genomic DNA. Translation: BAA16359.1.
PIRiH65023.
RefSeqiNP_416976.4. NC_000913.3.
WP_001336048.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75534; AAC75534; b2481.
BAA16359; BAA16359; BAA16359.
GeneIDi946959.
KEGGiecj:JW2466.
eco:b2481.
PATRICi32120349. VBIEscCol129921_2577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88563.1.
U00096 Genomic DNA. Translation: AAC75534.2.
AP009048 Genomic DNA. Translation: BAA16359.1.
PIRiH65023.
RefSeqiNP_416976.4. NC_000913.3.
WP_001336048.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259199. 13 interactions.
DIPiDIP-9983N.
IntActiP23481. 3 interactions.
STRINGi511145.b2481.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP23481.
PRIDEiP23481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75534; AAC75534; b2481.
BAA16359; BAA16359; BAA16359.
GeneIDi946959.
KEGGiecj:JW2466.
eco:b2481.
PATRICi32120349. VBIEscCol129921_2577.

Organism-specific databases

EchoBASEiEB1139.
EcoGeneiEG11150. hyfA.

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP23481.
KOiK12136.
OMAiKLCGLVC.
OrthoDBiEOG6XSZQF.
PhylomeDBiP23481.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-152.
ECOL316407:JW2466-MONOMER.

Miscellaneous databases

PROiP23481.

Family and domain databases

InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
[Graphical view]
PfamiPF00037. Fer4. 1 hit.
PF12798. Fer4_3. 1 hit.
[Graphical view]
PROSITEiPS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A molecular analysis of the 53.3 minute region of the Escherichia coli linkage map."
    Andrews S.C., Harrison P.M., Guest J.R.
    J. Gen. Microbiol. 137:361-367(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features."
    Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T., Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K., Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T.
    , Oyama S., Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H., Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.
    DNA Res. 4:91-113(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. Andrews S.C.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiHYFA_ECOLI
AccessioniPrimary (citable) accession number: P23481
Secondary accession number(s): P76565
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: May 11, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.