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Protein

Hydrogenase-4 component A

Gene

hyfA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Probable electron transfer protein for hydrogenase 4.1 Publication

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 4 [4Fe-4S] clusters.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi12Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi15Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi18Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi22Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi51Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi54Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi59Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi63Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi82Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi85Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi88Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi92Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi146Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi149Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi158Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi162Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-152.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydrogenase-4 component A (EC:1.-.-.-)
Gene namesi
Name:hyfA1 Publication
Synonyms:yffE
Ordered Locus Names:b2481, JW2466
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11150. hyfA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001592671 – 205Hydrogenase-4 component AAdd BLAST205

Proteomic databases

PaxDbiP23481.
PRIDEiP23481.

Expressioni

Inductioni

Most efficiently induced by formate during post-exponential growth at low external pH (pH 6.5) in the absence of respiratory electron acceptors O2+, NO3- or trimethylamine-N-oxide, i.e. under anaerobic control (PubMed:12426353, PubMed:14702328). Transcription is activated by FhlA and HyfR, inhibited by HycA, part of the sigma-54 (rpoN) regulon (PubMed:12426353). Subject to catabolite repression (PubMed:14702328). First member of a 10 gene operon (hyfABCDEFGHIJ); it is not clear if the 2 following genes (hydR-focB) are also in the operon (PubMed:12426353).2 Publications

Interactioni

Protein-protein interaction databases

BioGridi4259199. 13 interactors.
DIPiDIP-9983N.
IntActiP23481. 3 interactors.
STRINGi511145.b2481.

Structurei

3D structure databases

ProteinModelPortaliP23481.
SMRiP23481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 314Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST30
Domaini41 – 724Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST32
Domaini73 – 1024Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd BLAST30
Domaini140 – 1724Fe-4S ferredoxin-type 4PROSITE-ProRule annotationAdd BLAST33

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP23481.
KOiK12136.
OMAiKLCGLVC.
PhylomeDBiP23481.

Family and domain databases

InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
PfamiView protein in Pfam
PF00037. Fer4. 1 hit.
PF12798. Fer4_3. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.

Sequencei

Sequence statusi: Complete.

P23481-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRFVVAEPL WCTGCNTCLA ACSDVHKTQG LQQHPRLALA KTSTITAPVV
60 70 80 90 100
CHHCEEAPCL QVCPVNAISQ RDDAIQLNES LCIGCKLCAV VCPFGAISAS
110 120 130 140 150
GSRPVNAHAQ YVFQAEGSLK DGEENAPTQH ALLRWEPGVQ TVAVKCDLCD
160 170 180 190 200
FLPEGPACVR ACPNQALRLI TGDSLQRQMK EKQRLAASWF ANGGEDPLSL

TQEQR
Length:205
Mass (Da):22,154
Last modified:November 1, 1991 - v1
Checksum:iC06ED8FB0617B46B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88563.1.
U00096 Genomic DNA. Translation: AAC75534.2.
AP009048 Genomic DNA. Translation: BAA16359.1.
PIRiH65023.
RefSeqiNP_416976.4. NC_000913.3.
WP_001336048.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75534; AAC75534; b2481.
BAA16359; BAA16359; BAA16359.
GeneIDi946959.
KEGGiecj:JW2466.
eco:b2481.
PATRICi32120349. VBIEscCol129921_2577.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63654 Genomic DNA. Translation: AAB88563.1.
U00096 Genomic DNA. Translation: AAC75534.2.
AP009048 Genomic DNA. Translation: BAA16359.1.
PIRiH65023.
RefSeqiNP_416976.4. NC_000913.3.
WP_001336048.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP23481.
SMRiP23481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259199. 13 interactors.
DIPiDIP-9983N.
IntActiP23481. 3 interactors.
STRINGi511145.b2481.

Protein family/group databases

TCDBi3.D.1.9.1. the h(+) or na(+)-translocating nadh dehydrogenase (ndh) family.

Proteomic databases

PaxDbiP23481.
PRIDEiP23481.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75534; AAC75534; b2481.
BAA16359; BAA16359; BAA16359.
GeneIDi946959.
KEGGiecj:JW2466.
eco:b2481.
PATRICi32120349. VBIEscCol129921_2577.

Organism-specific databases

EchoBASEiEB1139.
EcoGeneiEG11150. hyfA.

Phylogenomic databases

eggNOGiENOG4108MFN. Bacteria.
COG1142. LUCA.
HOGENOMiHOG000163385.
InParanoidiP23481.
KOiK12136.
OMAiKLCGLVC.
PhylomeDBiP23481.

Enzyme and pathway databases

BioCyciEcoCyc:MONOMER0-152.

Miscellaneous databases

PROiPR:P23481.

Family and domain databases

InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
PfamiView protein in Pfam
PF00037. Fer4. 1 hit.
PF12798. Fer4_3. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 4 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiHYFA_ECOLI
AccessioniPrimary (citable) accession number: P23481
Secondary accession number(s): P76565
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 15, 2017
This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.