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Reviewed, UniProtKB/Swiss-Prot P23443 (KS6B1_HUMAN)

Last modified June 16, 2009. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Ribosomal protein S6 kinase beta-1
    EC=2.7.11.1
Alternative name(s):
    Ribosomal protein S6 kinase I
    S6K1
      Short name=S6K
    70 kDa ribosomal protein S6 kinase 1
    p70 S6 kinase alpha
      Short name=p70(S6K)-alpha
      Short name=p70-S6K
      Short name=P70S6K
      Short name=p70-alpha
Gene names
Name: RPS6KB1
Synonyms: STK14A
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Activation by serine/threonine phosphorylation and protein kinase C, inactivated by type 2A phosphatase.

Subunit structure

Interacts with PPP1R9A/neurabin-1 By similarity.

Subcellular location

Cytoplasm By similarity. Cell junction › synapse › synaptosome By similarity.

Tissue specificity

Widely expressed. Ref.2

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 protein kinase domain.

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Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]
Isoform Alpha I (identifier: P23443-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha II (identifier: P23443-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-23: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Ribosomal protein S6 kinase beta-1
PRO_0000024342

Regions

Domain91 – 352262Protein kinase
Domain353 – 42371AGC-kinase C-terminal
Nucleotide binding97 – 1059ATP By similarity

Sites

Active site2181Proton acceptor By similarity
Binding site1231ATP By similarity

Amino acid modifications

Modified residue4411Phosphoserine Ref.3 Ref.5
Modified residue4441Phosphothreonine Ref.3 Ref.5
Modified residue4471Phosphoserine Ref.3 Ref.5 Ref.4

Natural variations

Alternative sequence1 – 2323Missing in isoform Alpha II.
VSP_018839
Natural variant2251M → I Ref.7
VAR_040639
Natural variant2721R → C Ref.7
VAR_040640
Natural variant2761W → C Ref.7
VAR_040641
Natural variant2891G → E in a colorectal cancer sample; somatic mutation. Ref.6
VAR_035628
Natural variant3981S → A Ref.7
VAR_040642

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Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha I [UniParc].

Last modified October 11, 2004. Version 2.
Checksum: 2C3BA13CCDAF4AB3

FASTA52559,140
        10         20         30         40         50         60 
MRRRRRRDGF YPAPDFRDRE AEDMAGVFDI DLDQPEDAGS EDELEEGGQL NESMDHGGVG 

        70         80         90        100        110        120 
PYELGMEHCE KFEISETSVN RGPEKIRPEC FELLRVLGKG GYGKVFQVRK VTGANTGKIF 

       130        140        150        160        170        180 
AMKVLKKAMI VRNAKDTAHT KAERNILEEV KHPFIVDLIY AFQTGGKLYL ILEYLSGGEL 

       190        200        210        220        230        240 
FMQLEREGIF MEDTACFYLA EISMALGHLH QKGIIYRDLK PENIMLNHQG HVKLTDFGLC 

       250        260        270        280        290        300 
KESIHDGTVT HTFCGTIEYM APEILMRSGH NRAVDWWSLG ALMYDMLTGA PPFTGENRKK 

       310        320        330        340        350        360 
TIDKILKCKL NLPPYLTQEA RDLLKKLLKR NAASRLGAGP GDAGEVQAHP FFRHINWEEL 

       370        380        390        400        410        420 
LARKVEPPFK PLLQSEEDVS QFDSKFTRQT PVDSPDDSTL SESANQVFLG FTYVAPSVLE 

       430        440        450        460        470        480 
SVKEKFSFEP KIRSPRRFIG SPRTPVSPVK FSPGDFWGRG ASASTANPQT PVEYPMETSG 

       490        500        510        520 
IEQMDVTMSG EASAPLPIRQ PNSGPYKKQA FPMISKRPEH LRMNL

« Hide

Isoform Alpha II.

Checksum: 2DE6CF3FCABB9FD2
Show »

FASTA50256,189

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References

« Hide 'large scale' references
[1]"Cloning and expression of two human p70 S6 kinase polypeptides differing only at their amino termini."
Grove J., Bonerjee P., Balasubramanyam A., Coffer P., Price D.J., Avruch J., Woodgett J.R.
Mol. Cell. Biol. 11:5541-5550(1991) [PubMed: 1922062] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE INITIATION.
[2]"Molecular cloning and characterization of a novel p70 S6 kinase, p70 S6 kinase beta containing a proline-rich region."
Gout I., Minami T., Hara K., Tsujishita Y., Filonenko V., Waterfield M.D., Yonezawa K.
J. Biol. Chem. 273:30061-30064(1998) [PubMed: 9804755] [Abstract]
Cited for: TISSUE SPECIFICITY.
[3]"Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
Cell 127:635-648(2006) [PubMed: 17081983] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441; THR-444 AND SER-447, MASS SPECTROMETRY.
Tissue: Epithelium.
[4]"A probability-based approach for high-throughput protein phosphorylation analysis and site localization."
Beausoleil S.A., Villen J., Gerber S.A., Rush J., Gygi S.P.
Nat. Biotechnol. 24:1285-1292(2006) [PubMed: 16964243] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-447, MASS SPECTROMETRY.
Tissue: Epithelium.
[5]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-441; THR-444 AND SER-447, MASS SPECTROMETRY.
[6]"The consensus coding sequences of human breast and colorectal cancers."
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. expand/collapse author list , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.
Science 314:268-274(2006) [PubMed: 16959974] [Abstract]
Cited for: VARIANT [LARGE SCALE ANALYSIS] GLU-289.
[7]"Patterns of somatic mutation in human cancer genomes."
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G. expand/collapse author list , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
Nature 446:153-158(2007) [PubMed: 17344846] [Abstract]
Cited for: VARIANTS [LARGE SCALE ANALYSIS] ILE-225; CYS-272; CYS-276 AND ALA-398.
+Additional computationally mapped references.

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Cross-references

Sequence databases

M60724 mRNA. Translation: AAA36410.1.
M60725 mRNA. Translation: AAA36411.1.
IPIIPI00216132.
IPI00759649.
PIRA41687.
RefSeqNP_003152.1.
UniGeneHs.463642

3D structure databases

HSSPHSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBaseSearch...

PTM databases

PhosphoSiteP23443.

Proteomic databases

PRIDEP23443.

Genome annotation databases

EnsemblENSG00000108443. Homo sapiens. [Contig view]
GeneID6198.
KEGGhsa:6198.

Organism-specific databases

GeneCardsGC17P055325.
H-InvDBHIX0014049.
HGNCHGNC:10436. RPS6KB1.
HPACAB003838.
CAB018346.
MIM608938. gene.
PharmGKBPA34851.
GenAtlasSearch...

Phylogenomic databases

HOGENOMP23443.
HOVERGENP23443.
OMAP23443. DELEEGX.

Enzyme and pathway databases

BRENDA2.7.11.1. 247.
Pathway_Interaction_DBa6b1_a6b4_integrin_pathway. a6b1 and a6b4 Integrin signaling.
angiopoietinreceptor_pathway. Angiopoietin receptor Tie2-mediated signaling.
igf1_pathway. IGF1 pathway.
il2_pi3kpathway. IL2 signaling events mediated by PI3K.
il4_2pathway. IL4-mediated signaling events.
insulin_pathway. Insulin Pathway.
avb3_integrin_pathway. Integrins in angiogenesis.
mtor_4pathway. mTOR signaling pathway.
telomerasepathway. Regulation of Telomerase.
rxr_vdr_pathway. RXR and RAR hetrodimerization with other nuclear receptor.
met_pathway. Signaling events activated by Hepatocyte Growth Factor Receptor (c-Met).
kitpathway. Signaling events mediated by Stem cell factor receptor (c-Kit).
tgfbrpathway. TGF-beta receptor signaling.
ReactomeREACT_498. Signaling by Insulin receptor.

Gene expression databases

ArrayExpressP23443.
BgeeP23443.
CleanExHS_RPS6KB1.
GermOnlineENSG00000108443. Homo sapiens.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR017892. Pkinase_C.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR016238. Ribosomal_S6_kinase.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000605. Ribsml_S6_kin_1. 1 hit.
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio24073.
SOURCESearch...

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Entry information

Entry nameKS6B1_HUMAN
AccessionPrimary (citable) accession number: P23443
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: October 11, 2004
Last modified: June 16, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents