Homeobox protein Nkx-2.1 - Rattus norvegicus (Rat)

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Reviewed, UniProtKB/Swiss-Prot P23441 (NKX21_RAT)

Last modified October 13, 2009. Version 88. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Homeobox protein Nkx-2.1
Alternative name(s):
    Thyroid transcription factor 1
      Short name=TTF-1
    Thyroid nuclear factor 1

Gene names

Name:	Nkx2-1
Synonyms:	Nkx-2.1, Titf1, Ttf-1, Ttf1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Transcription factor that binds and activates the promoter of thyroid specific genes such as thyroglobulin, thyroperoxidase, and thyrotropin receptor. Crucial in the maintenance of the thyroid differentiation phenotype. May play a role in lung development and surfactant homeostasis.
Subcellular location	Nucleus.
Tissue specificity	Thyroid, lung and CNS. Expressed in restricted regions of the developing brain within the diencephalon, in parts of the hypothalamus and neurohypophysis, and in the telencephalon.
Post-translational modification	Phosphorylated on serine residues by STK3. Ref.3
Sequence similarities	Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.

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Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	development of primary female sexual characteristics Inferred from expression pattern. Source: RGD feeding behavior Inferred from mutant phenotype. Source: RGD hyperosmotic salinity response Inferred from direct assay. Source: RGD positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay. Source: MGI regulation of blood volume by renin-angiotensin Inferred from direct assay. Source: RGD response to ethanol Inferred from expression pattern. Source: RGD response to lipopolysaccharide Inferred from direct assay. Source: RGD transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB transcription activator activity Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
Pax8	P51974	2	EBI-1223127,EBI-1223113
Wwtr1	Q9EPK5	1	EBI-1223127,EBI-1211920	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 372

372

Homeobox protein Nkx-2.1

PRO_0000049345

Regions

DNA binding

161 – 220

Homeobox

Compositional bias

234 – 244

Poly-Gly

Compositional bias

247 – 254

Poly-Gln

Compositional bias

295 – 304

Poly-Ala

Secondary structure

372

Helix Strand Turn

Details...

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Sequences

Sequence

Length

Mass (Da)

Tools

P23441-1 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 8694421DA25D4135
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FASTA

372

38,554

        10         20         30         40         50         60 
MSMSPKHTTP FSVSDILSPL EESYKKVGME GGGLGAPLAA YRQGQAAPPA AAMQQHAVGH 

        70         80         90        100        110        120 
HGAVTAAYHM TAAGVPQLSH SAVGGYCNGN LGNMSELPPY QDTMRNSASG PGWYGANPDP 

       130        140        150        160        170        180 
RFPAISRFMG PASGMNMSGM GGLGSLGDVS KNMAPLPSAP RRKRRVLFSQ AQVYELERRF 

       190        200        210        220        230        240 
KQQKYLSAPE REHLASMIHL TPTQVKIWFQ NHRYKMKRQA KDKAAQQQLQ QDSGGGGGGG 

       250        260        270        280        290        300 
GGAGCPQQQQ AQQQSPRRVA VPVLVKDGKP CQAGAPAPGA ASLQGHAQQQ AQQQAQAAQA 

       310        320        330        340        350        360 
AAAAISVGSG GAGLGAHPGH QPGSAGQSPD LAHHAASPAA LQGQVSSLSH LNSSGSDYGA 

       370 
MSCSTLLYGR TW

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References

[1]	"Thyroid nuclear factor 1 (TTF-1) contains a homeodomain and displays a novel DNA binding specificity." Guazzi S., Price M., de Felice M., Damante G., Mattei M.-G., di Lauro R. EMBO J. 9:3631-3639(1990) [PubMed: 1976511] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Structure of the rat thyroid transcription factor-1 (TTF-1) gene." Endo T., Ohta K., Saito T., Haraguchi K., Nakazato M., Kogai T., Onaya T. Biochem. Biophys. Res. Commun. 204:1358-1363(1994) [PubMed: 7980615] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[3]	"Identification of the thyroid transcription factor 1 as a target for rat MST2 kinase." Aurisicchio L., Dilauro R., Zannini M. J. Biol. Chem. 273:1477-1482(1998) [PubMed: 9430685] [Abstract] Cited for: PHOSPHORYLATION.
[4]	"Structural study of rat thyroid transcription factor 1 homeodomain (TTF-1 HD) by nuclear magnetic resonance." Viglino P., Fogolari F., Formisiano S., Bortolotti N., Damante G., di Lauro R., Esposito G. FEBS Lett. 336:397-402(1993) [PubMed: 8282100] [Abstract] Cited for: STRUCTURE BY NMR OF 161-227.
[5]	"Analysis of the solution structure of the homeodomain of rat thyroid transcription factor 1 by 1H-NMR spectroscopy and restrained molecular mechanics." Esposito G., Fogolari F., Damante G., Formisano S., Tell G., Leonardi A., di Lauro R., Viglino P. Eur. J. Biochem. 241:101-113(1996) [PubMed: 8898894] [Abstract] Cited for: STRUCTURE BY NMR OF 161-227.
+	Additional computationally mapped references.

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Cross-references

Sequence databases

X53858 mRNA. Translation: CAA37851.1.
D38035 Genomic DNA. Translation: BAA07231.1. Sequence problems.

IPI

IPI00554071.

PIR

S12002.

UniGene

Rn.34265

3D structure databases

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1FTT	NMR	-	A	161-227	[»]

DisProt

DP00071.

ModBase

Search...

Protein-protein interaction databases

IntAct

P23441. 2 interactions.

STRING

P23441.

PTM databases

PhosphoSite

P23441.

Genome annotation databases

Ensembl

ENSRNOT00000011453; ENSRNOP00000011453; ENSRNOG00000008644; Rattus norvegicus. [Genome view]

UCSC

X53858. rat.

Organism-specific databases

RGD

3866. Nkx2-1.

Phylogenomic databases

HOVERGEN

P23441.

Gene expression databases

ArrayExpress

P23441.

Genevestigator

P23441.

GermOnline

ENSRNOG00000008644. Rattus norvegicus.

Family and domain databases

InterPro

IPR001356. Homeobox.
IPR017970. Homeobox_CS.
IPR020479. Homeobox_region.
IPR012287. Homeodomain-rel.
[Graphical view]

Gene3D

G3DSA:1.10.10.60. Homeodomain-rel. 1 hit.

Pfam

PF00046. Homeobox. 1 hit.
[Graphical view]

PRINTS

PR00024. HOMEOBOX.

ProDom

PD000010. Homeobox. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00389. HOX. 1 hit.
[Graphical view]

PROSITE

PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

ProtoNet

Search...

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Entry information

Entry name

NKX21_RAT

Accession

Primary (citable) accession number: P23441
Secondary accession number(s): O08630, O70121

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	October 13, 2009
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents