Reviewed, UniProtKB/Swiss-Prot P23440 (PDE6B_MOUSE)

Names and origin

Protein names

Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
      Short name=GMP-PDE beta
    EC=3.1.4.35

Gene names

Name:	Pde6b
Synonyms:	Mpb, Pdeb, rd

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	856 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Membrane; Lipid-anchor.
Involvement in disease	Defects in Pde6b are the cause of retinal degeneration.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Lipoprotein Prenylation
Gene Ontology (GO)
Biological process	detection of light stimulus Inferred from mutant phenotype. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P23440-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P23440-2)

Also known as: Beta';

The sequence of this isoform differs from the canonical sequence as follows:
     801-856: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 853	853	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta		PRO_0000023350
	Propeptide	854 – 856	3	Removed in mature form By similarity		PRO_0000023351
Regions
	Domain	71 – 220	150	GAF 1
	Domain	252 – 429	178	GAF 2
Amino acid modifications
	Lipidation	853	1	S-geranylgeranyl cysteine By similarity
Natural variations
	Alternative sequence	801 – 856	56	Missing in isoform 2.		VSP_004592
Experimental info
	Sequence conflict	5	1	G → E in CAA42719. Ref.2
	Sequence conflict	19	1	S → A in CAA42719. Ref.2
	Sequence conflict	49 – 50	2	DV → EL in CAA42719. Ref.2
	Sequence conflict	158	1	T → P in CAA42719. Ref.2
	Sequence conflict	176	1	C → L in CAA42719. Ref.2
	Sequence conflict	232	1	R → E in CAA42719. Ref.2
	Sequence conflict	236	1	S → G in CAA42719. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified April 1, 1993. Version 2. Checksum: 3677704D0C7496D1	FASTA	856	98,502
	10 20 30 40 50 60 MSLSGEQVRS FLDGNPTFSH QYFGKKLSPE NVAGACEDGW LADCGSLRDV CQVEESAALF 70 80 90 100 110 120 ELVQDMQESV NMERVVFKIL RRLCTILHAD RCSLFMYRQR NGIAELATRL FSVQPDSLLE 130 140 150 160 170 180 DCLVPPDSEI VFPLDIGIVG HVAQTKKMIN VQDVAECTHF SSFADELTDY VTKNICSTPI 190 200 210 220 230 240 MNGKDVVAVI MAVNKLDGPC FTSEDEDVFT KYLNFATLNL KIYHLSYLHN CRTRRSQVLL 250 260 270 280 290 300 WSANKVFEEL TDIERQFHKA FYTVRAYLNC ERYSVGLLDM TKEKEFFDVW PVLMGEAQPY 310 320 330 340 350 360 SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PTPPADHWAL ASGLPTYVAE SGFICNIMNA 370 380 390 400 410 420 SADEMFNFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DDQDEVLMES 430 440 450 460 470 480 LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDK DEIQEILPTR DRLGKEPADC 490 500 510 520 530 540 EEDELGKILK EELPGPTKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 550 560 570 580 590 600 VRFLFSVSKA YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID 610 620 630 640 650 660 HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLAEE SLNIYQNLNR RQHEHVIHLM 670 680 690 700 710 720 DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DKKSWVEYLS LETTRKEIVM AMMMTACDLS 730 740 750 760 770 780 AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 790 800 810 820 830 840 FVYKEFSRFH EEILPMFDRL QNNRKEWKAL ADEYEAKVKA LEEEKKKEED RVAAKKVGTE 850 VCNGGPAPKS STCCIL
	Isoform 2 (Beta'). Checksum: 7517B29BEF75C839	FASTA	800	92,298
	10 20 30 40 50 60 MSLSGEQVRS FLDGNPTFSH QYFGKKLSPE NVAGACEDGW LADCGSLRDV CQVEESAALF 70 80 90 100 110 120 ELVQDMQESV NMERVVFKIL RRLCTILHAD RCSLFMYRQR NGIAELATRL FSVQPDSLLE 130 140 150 160 170 180 DCLVPPDSEI VFPLDIGIVG HVAQTKKMIN VQDVAECTHF SSFADELTDY VTKNICSTPI 190 200 210 220 230 240 MNGKDVVAVI MAVNKLDGPC FTSEDEDVFT KYLNFATLNL KIYHLSYLHN CRTRRSQVLL 250 260 270 280 290 300 WSANKVFEEL TDIERQFHKA FYTVRAYLNC ERYSVGLLDM TKEKEFFDVW PVLMGEAQPY 310 320 330 340 350 360 SGPRTPDGRE IVFYKVIDYI LHGKEDIKVI PTPPADHWAL ASGLPTYVAE SGFICNIMNA 370 380 390 400 410 420 SADEMFNFQE GPLDDSGWVI KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DDQDEVLMES 430 440 450 460 470 480 LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDK DEIQEILPTR DRLGKEPADC 490 500 510 520 530 540 EEDELGKILK EELPGPTKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 550 560 570 580 590 600 VRFLFSVSKA YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID 610 620 630 640 650 660 HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLAEE SLNIYQNLNR RQHEHVIHLM 670 680 690 700 710 720 DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DKKSWVEYLS LETTRKEIVM AMMMTACDLS 730 740 750 760 770 780 AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 790 800 FVYKEFSRFH EEILPMFDRL

References

[1]	"Retinal degeneration in the rd mouse is caused by a defect in the beta subunit of rod cGMP-phosphodiesterase." Bowes C., Li T., Danciger M., Baxter L.C., Applebury M.L., Farber D.B. Nature 347:677-680(1990) [PubMed: 1977087] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Retina.
[2]	"Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene." Baehr W., Champagne M.S., Lee A.K., Pittler S.J. FEBS Lett. 278:107-114(1991) [PubMed: 1847109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X55968 mRNA. Translation: CAA39439.1. Sequence problems. X60133 mRNA. Translation: CAA42719.1.
IPI	IPI00132326. IPI00227887.
PIR	S30762.
UniGene	Mm.1372
3D structure databases
ModBase	Search...
PTM databases
PhosphoSite	P23440.
Genome annotation databases
Ensembl	ENSMUSG00000029491. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:97525. Pde6b.
Phylogenomic databases
HOGENOM	P23440.
HOVERGEN	P23440.
Enzyme and pathway databases
BRENDA	3.1.4.35. 244.
Gene expression databases
ArrayExpress	P23440.
Bgee	P23440.
GermOnline	ENSMUSG00000029491. Mus musculus.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PDE6B_MOUSE

Accession

Primary (citable) accession number: P23440

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)