Reviewed, UniProtKB/Swiss-Prot P23439 (PDE6B_BOVIN)

Names and origin

Protein names

Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta
      Short name=GMP-PDE beta
    EC=3.1.4.35

Gene names

Name:	PDE6B
Synonyms:	PDEB

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	853 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Membrane; Lipid-anchor.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Acetylation Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 850	849	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta		PRO_0000023344
	Propeptide	851 – 853	3	Removed in mature form By similarity		PRO_0000023345
Regions
	Domain	71 – 220	150	GAF 1
	Domain	252 – 429	178	GAF 2
Sites
	Active site	557	1	Proton donor By similarity
	Metal binding	561	1	Divalent metal cation 1 By similarity
	Metal binding	597	1	Divalent metal cation 1 By similarity
	Metal binding	598	1	Divalent metal cation 1 By similarity
	Metal binding	598	1	Divalent metal cation 2 By similarity
	Metal binding	718	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylserine
	Modified residue	850	1	Cysteine methyl ester By similarity
	Lipidation	850	1	S-geranylgeranyl cysteine By similarity
Experimental info
	Sequence conflict	483 – 484	2	DE → EQ Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P23439-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C4B3F22CFFE7F2FB	FASTA	853	98,331
	10 20 30 40 50 60 MSLSEGQVHR FLDQNPGFAD QYFGRKLSPE DVANACEDGC PEGCTSFREL CQVEESAALF 70 80 90 100 110 120 ELVQDMQENV NMERVVFKIL RRLCSILHAD RCSLFMYRQR NGVAELATRL FSVQPDSVLE 130 140 150 160 170 180 DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN VQDVMECPHF SSFADELTDY VTRNILATPI 190 200 210 220 230 240 MNGKDVVAVI MAVNKLDGPC FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL 250 260 270 280 290 300 WSANKVFEEL TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQAY 310 320 330 340 350 360 SGPRTPDGRE ILFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE SGFICNIMNA 370 380 390 400 410 420 PADEMFNFQE GPLDDSGWIV KNVLSMPIVN KKEEIVGVAT FYNRKDGKPF DEQDEVLMES 430 440 450 460 470 480 LTQFLGWSVL NTDTYDKMNK LENRKDIAQD MVLYHVRCDR EEIQLILPTR ERLGKEPADC 490 500 510 520 530 540 EEDELGKILK EVLPGPAKFD IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL 550 560 570 580 590 600 VRFLFSVSKG YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID 610 620 630 640 650 660 HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR RQHEHVIHLM 670 680 690 700 710 720 DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS LETTRKEIVM AMMMTACDLS 730 740 750 760 770 780 AITKPWEVQS KVALLVAAEF WEQGDLERTV LDQQPIPMMD RNKAAELPKL QVGFIDFVCT 790 800 810 820 830 840 FVYKEFSRFH EEILPMFDRL QNNRKEWKAL ADEYEAKVKA LEEDQKKETT AKKVGTEICN 850 GGPAPRSSTC RIL

References

[1]	"Beta-subunit of bovine rod photoreceptor cGMP phosphodiesterase. Comparison with the phosphodiesterase family." Lipkin V.M., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Gubanov V.V., Li T., Johnston J.P., Volpp K.J., Applebury M.L. J. Biol. Chem. 265:12955-12959(1990) [PubMed: 2165490] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of beta-subunit and nucleotide sequence of corresponding cDNA." Lipkin V.M., Gubanov V.V., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Shuvaeva T.M., Surina E.A., Zagranichny V.E., Li T. Bioorg. Khim. 16:118-120(1990) [PubMed: 2161230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	J05553 mRNA. Translation: AAA30440.1. X57146 mRNA. Translation: CAA40436.1.
IPI	IPI00711026.
PIR	A36617.
RefSeq	NP_776843.1.
UniGene	Bt.55
3D structure databases
SMR	P23439. Positions 481-813.
ModBase	Search...
Protein-protein interaction databases
STRING	P23439.
Genome annotation databases
Ensembl	ENSBTAT00000023239; ENSBTAP00000023239; ENSBTAG00000017480; Bos taurus. [Genome view]
GeneID	281974.
KEGG	bta:281974.
Organism-specific databases
CTD	281974.
Phylogenomic databases
eggNOG	maNOG07458.
HOVERGEN	P23439.
InParanoid	P23439.
Enzyme and pathway databases
BRENDA	3.1.4.35. 251.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PDE6B_BOVIN

Accession

Primary (citable) accession number: P23439

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 19, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)