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P23439

- PDE6B_BOVIN

UniProt

P23439 - PDE6B_BOVIN

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Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Gene

PDE6B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei557 – 5571Proton donorBy similarity
Metal bindingi561 – 5611Divalent metal cation 1By similarity
Metal bindingi597 – 5971Divalent metal cation 1By similarity
Metal bindingi598 – 5981Divalent metal cation 1By similarity
Metal bindingi598 – 5981Divalent metal cation 2By similarity
Metal bindingi718 – 7181Divalent metal cation 1By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. signal transduction Source: InterPro
  2. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_211562. Activation of the phototransduction cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)
Short name:
GMP-PDE beta
Gene namesi
Name:PDE6B
Synonyms:PDEB
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. photoreceptor disc membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed
Chaini2 – 850849Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit betaPRO_0000023344Add
BLAST
Propeptidei851 – 8533Removed in mature formBy similarityPRO_0000023345

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine
Modified residuei850 – 8501Cysteine methyl esterBy similarity
Lipidationi850 – 8501S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

PRIDEiP23439.

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

IntActiP23439. 2 interactions.
MINTiMINT-6824892.
STRINGi9913.ENSBTAP00000023239.

Structurei

3D structure databases

ProteinModelPortaliP23439.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini71 – 220150GAF 1Add
BLAST
Domaini252 – 429178GAF 2Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG242608.
HOGENOMiHOG000007069.
HOVERGENiHBG053539.
InParanoidiP23439.
KOiK13756.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23439-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSEGQVHR FLDQNPGFAD QYFGRKLSPE DVANACEDGC PEGCTSFREL
60 70 80 90 100
CQVEESAALF ELVQDMQENV NMERVVFKIL RRLCSILHAD RCSLFMYRQR
110 120 130 140 150
NGVAELATRL FSVQPDSVLE DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN
160 170 180 190 200
VQDVMECPHF SSFADELTDY VTRNILATPI MNGKDVVAVI MAVNKLDGPC
210 220 230 240 250
FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL
260 270 280 290 300
TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQAY
310 320 330 340 350
SGPRTPDGRE ILFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE
360 370 380 390 400
SGFICNIMNA PADEMFNFQE GPLDDSGWIV KNVLSMPIVN KKEEIVGVAT
410 420 430 440 450
FYNRKDGKPF DEQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD
460 470 480 490 500
MVLYHVRCDR EEIQLILPTR ERLGKEPADC EEDELGKILK EVLPGPAKFD
510 520 530 540 550
IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKG
560 570 580 590 600
YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID
610 620 630 640 650
HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR
660 670 680 690 700
RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS
710 720 730 740 750
LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV
760 770 780 790 800
LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL
810 820 830 840 850
QNNRKEWKAL ADEYEAKVKA LEEDQKKETT AKKVGTEICN GGPAPRSSTC

RIL
Length:853
Mass (Da):98,331
Last modified:November 1, 1991 - v1
Checksum:iC4B3F22CFFE7F2FB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti483 – 4842DE → EQ(PubMed:2161230)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05553 mRNA. Translation: AAA30440.1.
X57146 mRNA. Translation: CAA40436.1.
PIRiA36617.
RefSeqiNP_776843.1. NM_174418.1.
UniGeneiBt.55.

Genome annotation databases

GeneIDi281974.
KEGGibta:281974.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J05553 mRNA. Translation: AAA30440.1 .
X57146 mRNA. Translation: CAA40436.1 .
PIRi A36617.
RefSeqi NP_776843.1. NM_174418.1.
UniGenei Bt.55.

3D structure databases

ProteinModelPortali P23439.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P23439. 2 interactions.
MINTi MINT-6824892.
STRINGi 9913.ENSBTAP00000023239.

Chemistry

ChEMBLi CHEMBL2096979.

Proteomic databases

PRIDEi P23439.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281974.
KEGGi bta:281974.

Organism-specific databases

CTDi 5158.

Phylogenomic databases

eggNOGi NOG242608.
HOGENOMi HOG000007069.
HOVERGENi HBG053539.
InParanoidi P23439.
KOi K13756.

Enzyme and pathway databases

Reactomei REACT_211562. Activation of the phototransduction cascade.

Miscellaneous databases

NextBioi 20805845.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Beta-subunit of bovine rod photoreceptor cGMP phosphodiesterase. Comparison with the phosphodiesterase family."
    Lipkin V.M., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Gubanov V.V., Li T., Johnston J.P., Volpp K.J., Applebury M.L.
    J. Biol. Chem. 265:12955-12959(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of beta-subunit and nucleotide sequence of corresponding cDNA."
    Lipkin V.M., Gubanov V.V., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Shuvaeva T.M., Surina E.A., Zagranichny V.E., Li T.
    Bioorg. Khim. 16:118-120(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiPDE6B_BOVIN
AccessioniPrimary (citable) accession number: P23439
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 26, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3