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P23439

- PDE6B_BOVIN

UniProt

P23439 - PDE6B_BOVIN

Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta

Gene

PDE6B

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    This protein participates in processes of transmission and amplification of the visual signal. Necessary for the formation of a functional phosphodiesterase holoenzyme.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei557 – 5571Proton donorBy similarity
    Metal bindingi561 – 5611Divalent metal cation 1By similarity
    Metal bindingi597 – 5971Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 1By similarity
    Metal bindingi598 – 5981Divalent metal cation 2By similarity
    Metal bindingi718 – 7181Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. signal transduction Source: InterPro
    2. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_211562. Activation of the phototransduction cascade.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta (EC:3.1.4.35)
    Short name:
    GMP-PDE beta
    Gene namesi
    Name:PDE6B
    Synonyms:PDEB
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. photoreceptor disc membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed
    Chaini2 – 850849Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit betaPRO_0000023344Add
    BLAST
    Propeptidei851 – 8533Removed in mature formBy similarityPRO_0000023345

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine
    Modified residuei850 – 8501Cysteine methyl esterBy similarity
    Lipidationi850 – 8501S-geranylgeranyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    PRIDEiP23439.

    Interactioni

    Subunit structurei

    Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

    Protein-protein interaction databases

    IntActiP23439. 2 interactions.
    MINTiMINT-6824892.
    STRINGi9913.ENSBTAP00000023239.

    Structurei

    3D structure databases

    ProteinModelPortaliP23439.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini71 – 220150GAF 1Add
    BLAST
    Domaini252 – 429178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG242608.
    HOGENOMiHOG000007069.
    HOVERGENiHBG053539.
    InParanoidiP23439.
    KOiK13756.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23439-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLSEGQVHR FLDQNPGFAD QYFGRKLSPE DVANACEDGC PEGCTSFREL    50
    CQVEESAALF ELVQDMQENV NMERVVFKIL RRLCSILHAD RCSLFMYRQR 100
    NGVAELATRL FSVQPDSVLE DCLVPPDSEI VFPLDIGVVG HVAQTKKMVN 150
    VQDVMECPHF SSFADELTDY VTRNILATPI MNGKDVVAVI MAVNKLDGPC 200
    FTSEDEDVFL KYLNFGTLNL KIYHLSYLHN CETRRGQVLL WSANKVFEEL 250
    TDIERQFHKA FYTVRAYLNC DRYSVGLLDM TKEKEFFDVW PVLMGEAQAY 300
    SGPRTPDGRE ILFYKVIDYI LHGKEDIKVI PSPPADHWAL ASGLPTYVAE 350
    SGFICNIMNA PADEMFNFQE GPLDDSGWIV KNVLSMPIVN KKEEIVGVAT 400
    FYNRKDGKPF DEQDEVLMES LTQFLGWSVL NTDTYDKMNK LENRKDIAQD 450
    MVLYHVRCDR EEIQLILPTR ERLGKEPADC EEDELGKILK EVLPGPAKFD 500
    IYEFHFSDLE CTELELVKCG IQMYYELGVV RKFQIPQEVL VRFLFSVSKG 550
    YRRITYHNWR HGFNVAQTMF TLLMTGKLKS YYTDLEAFAM VTAGLCHDID 600
    HRGTNNLYQM KSQNPLAKLH GSSILERHHL EFGKFLLSEE TLNIYQNLNR 650
    RQHEHVIHLM DIAIIATDLA LYFKKRTMFQ KIVDESKNYE DRKSWVEYLS 700
    LETTRKEIVM AMMMTACDLS AITKPWEVQS KVALLVAAEF WEQGDLERTV 750
    LDQQPIPMMD RNKAAELPKL QVGFIDFVCT FVYKEFSRFH EEILPMFDRL 800
    QNNRKEWKAL ADEYEAKVKA LEEDQKKETT AKKVGTEICN GGPAPRSSTC 850
    RIL 853
    Length:853
    Mass (Da):98,331
    Last modified:November 1, 1991 - v1
    Checksum:iC4B3F22CFFE7F2FB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti483 – 4842DE → EQ(PubMed:2161230)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05553 mRNA. Translation: AAA30440.1.
    X57146 mRNA. Translation: CAA40436.1.
    PIRiA36617.
    RefSeqiNP_776843.1. NM_174418.1.
    UniGeneiBt.55.

    Genome annotation databases

    GeneIDi281974.
    KEGGibta:281974.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J05553 mRNA. Translation: AAA30440.1 .
    X57146 mRNA. Translation: CAA40436.1 .
    PIRi A36617.
    RefSeqi NP_776843.1. NM_174418.1.
    UniGenei Bt.55.

    3D structure databases

    ProteinModelPortali P23439.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P23439. 2 interactions.
    MINTi MINT-6824892.
    STRINGi 9913.ENSBTAP00000023239.

    Chemistry

    BindingDBi P23439.
    ChEMBLi CHEMBL2096979.

    Proteomic databases

    PRIDEi P23439.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281974.
    KEGGi bta:281974.

    Organism-specific databases

    CTDi 5158.

    Phylogenomic databases

    eggNOGi NOG242608.
    HOGENOMi HOG000007069.
    HOVERGENi HBG053539.
    InParanoidi P23439.
    KOi K13756.

    Enzyme and pathway databases

    Reactomei REACT_211562. Activation of the phototransduction cascade.

    Miscellaneous databases

    NextBioi 20805845.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Beta-subunit of bovine rod photoreceptor cGMP phosphodiesterase. Comparison with the phosphodiesterase family."
      Lipkin V.M., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Gubanov V.V., Li T., Johnston J.P., Volpp K.J., Applebury M.L.
      J. Biol. Chem. 265:12955-12959(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cyclic GMP phosphodiesterase from bovine retina. Amino acid sequence of beta-subunit and nucleotide sequence of corresponding cDNA."
      Lipkin V.M., Gubanov V.V., Khramtsov N.V., Vasilevskaya I.A., Atabekova N.V., Muradov K.G., Shuvaeva T.M., Surina E.A., Zagranichny V.E., Li T.
      Bioorg. Khim. 16:118-120(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiPDE6B_BOVIN
    AccessioniPrimary (citable) accession number: P23439
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3