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Protein

Cyclin-dependent kinase 2

Gene

cdk2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the control of the cell cycle. Interacts with cyclins A, B, D, or E. Activity of CDK2 is maximal during S phase and G2.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it (By similarity). Activated by spdya.By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei33 – 331ATPPROSITE-ProRule annotation
Binding sitei86 – 861ATPPROSITE-ProRule annotation
Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation
Binding sitei145 – 1451ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation
Nucleotide bindingi81 – 833ATPPROSITE-ProRule annotation
Nucleotide bindingi129 – 1324ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.22. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-dependent kinase 2 (EC:2.7.11.22)
Alternative name(s):
CDC2 homolog Eg1 protein kinase
Cell division protein kinase 2
Gene namesi
Name:cdk2
Synonyms:eg1
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-1001995. cdk2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 297297Cyclin-dependent kinase 2PRO_0000085774Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei14 – 141PhosphothreonineBy similarity
Modified residuei15 – 151PhosphotyrosineBy similarity
Modified residuei160 – 1601Phosphothreonine; by CAK1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP23437.

Expressioni

Developmental stagei

Synthesized in unfertilized egg, but no longer made in the early embryo.1 Publication

Interactioni

Subunit structurei

Interacts with spdya.1 Publication

Protein-protein interaction databases

BioGridi100639. 5 interactions.
DIPiDIP-60879N.

Structurei

3D structure databases

ProteinModelPortaliP23437.
SMRiP23437. Positions 1-296.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini4 – 286283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG014652.
KOiK02206.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENFQKVEKI GEGTYGVVYK ARNRETGEIV ALKKIRLDTE TEGVPSTAIR
60 70 80 90 100
EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLNQDLKKF MDGSNISGIS
110 120 130 140 150
LALVKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINSDGA IKLADFGLAR
160 170 180 190 200
AFGVPVRTFT HEVVTLWYRA PEILLGCKFY STAVDIWSLG CIFAEMITRR
210 220 230 240 250
ALFPGDSEID QLFRIFRTLG TPDEVSWPGV TTMPDYKSTF PKWIRQDFSK
260 270 280 290
VVPPLDEDGR DLLAQMLQYD SNKRISAKVA LTHPFFRDVS RPTPHLI
Length:297
Mass (Da):33,870
Last modified:May 2, 2006 - v3
Checksum:i173ED4E4C12DA397
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931G → R in CAA32443 (PubMed:1704128).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14227 mRNA. Translation: CAA32443.1.
BC106636 mRNA. Translation: AAI06637.1.
PIRiA37871.
RefSeqiNP_001084120.1. NM_001090651.1.
UniGeneiXl.4227.

Genome annotation databases

GeneIDi399314.
KEGGixla:399314.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X14227 mRNA. Translation: CAA32443.1.
BC106636 mRNA. Translation: AAI06637.1.
PIRiA37871.
RefSeqiNP_001084120.1. NM_001090651.1.
UniGeneiXl.4227.

3D structure databases

ProteinModelPortaliP23437.
SMRiP23437. Positions 1-296.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi100639. 5 interactions.
DIPiDIP-60879N.

Proteomic databases

PRIDEiP23437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi399314.
KEGGixla:399314.

Organism-specific databases

CTDi1017.
XenbaseiXB-GENE-1001995. cdk2.

Phylogenomic databases

HOVERGENiHBG014652.
KOiK02206.

Enzyme and pathway databases

BRENDAi2.7.11.22. 6725.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2."
    Paris J., le Guellec R., Couturier A., le Guellec K., Omilli F., Camonis J., Macneill S., Philippe M.
    Proc. Natl. Acad. Sci. U.S.A. 88:1039-1043(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
    Tissue: Ovary.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Oocyte.
  3. "The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2."
    Poon R.Y.C., Yamashita K., Adamczewski J.P., Hunt T., Shuttleworth J.
    EMBO J. 12:3123-3132(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT THR-160.
  4. "Speedy: a novel cell cycle regulator of the G2/M transition."
    Lenormand J.-L., Dellinger R.W., Knudsen K.E., Subramani S., Donoghue D.J.
    EMBO J. 18:1869-1877(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SPDYA, ENZYME REGULATION.
    Tissue: Ovary.

Entry informationi

Entry nameiCDK2_XENLA
AccessioniPrimary (citable) accession number: P23437
Secondary accession number(s): Q3KPN7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: May 2, 2006
Last modified: April 1, 2015
This is version 100 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.