Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P23437

- CDK2_XENLA

UniProt

P23437 - CDK2_XENLA

Protein

Cyclin-dependent kinase 2

Gene

cdk2

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 3 (02 May 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the control of the cell cycle. Interacts with cyclins A, B, D, or E. Activity of CDK2 is maximal during S phase and G2.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it By similarity. Activated by spdya.By similarity1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Binding sitei86 – 861ATPPROSITE-ProRule annotation
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation
    Binding sitei145 – 1451ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation
    Nucleotide bindingi81 – 833ATPPROSITE-ProRule annotation
    Nucleotide bindingi129 – 1324ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    3. protein binding Source: UniProtKB

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. regulation of cell cycle Source: GOC

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.22. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 2 (EC:2.7.11.22)
    Alternative name(s):
    CDC2 homolog Eg1 protein kinase
    Cell division protein kinase 2
    Gene namesi
    Name:cdk2
    Synonyms:eg1
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-1001995. cdk2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 297297Cyclin-dependent kinase 2PRO_0000085774Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei14 – 141PhosphothreonineBy similarity
    Modified residuei15 – 151PhosphotyrosineBy similarity
    Modified residuei160 – 1601Phosphothreonine; by CAK1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PRIDEiP23437.

    Expressioni

    Developmental stagei

    Synthesized in unfertilized egg, but no longer made in the early embryo.1 Publication

    Interactioni

    Subunit structurei

    Interacts with spdya.1 Publication

    Protein-protein interaction databases

    BioGridi100639. 5 interactions.
    DIPiDIP-60879N.

    Structurei

    3D structure databases

    ProteinModelPortaliP23437.
    SMRiP23437. Positions 1-296.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 286283Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    HOVERGENiHBG014652.
    KOiK02206.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23437-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENFQKVEKI GEGTYGVVYK ARNRETGEIV ALKKIRLDTE TEGVPSTAIR    50
    EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLNQDLKKF MDGSNISGIS 100
    LALVKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINSDGA IKLADFGLAR 150
    AFGVPVRTFT HEVVTLWYRA PEILLGCKFY STAVDIWSLG CIFAEMITRR 200
    ALFPGDSEID QLFRIFRTLG TPDEVSWPGV TTMPDYKSTF PKWIRQDFSK 250
    VVPPLDEDGR DLLAQMLQYD SNKRISAKVA LTHPFFRDVS RPTPHLI 297
    Length:297
    Mass (Da):33,870
    Last modified:May 2, 2006 - v3
    Checksum:i173ED4E4C12DA397
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti93 – 931G → R in CAA32443. (PubMed:1704128)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14227 mRNA. Translation: CAA32443.1.
    BC106636 mRNA. Translation: AAI06637.1.
    PIRiA37871.
    RefSeqiNP_001084120.1. NM_001090651.1.
    UniGeneiXl.4227.

    Genome annotation databases

    GeneIDi399314.
    KEGGixla:399314.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X14227 mRNA. Translation: CAA32443.1 .
    BC106636 mRNA. Translation: AAI06637.1 .
    PIRi A37871.
    RefSeqi NP_001084120.1. NM_001090651.1.
    UniGenei Xl.4227.

    3D structure databases

    ProteinModelPortali P23437.
    SMRi P23437. Positions 1-296.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 100639. 5 interactions.
    DIPi DIP-60879N.

    Proteomic databases

    PRIDEi P23437.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 399314.
    KEGGi xla:399314.

    Organism-specific databases

    CTDi 1017.
    Xenbasei XB-GENE-1001995. cdk2.

    Phylogenomic databases

    HOVERGENi HBG014652.
    KOi K02206.

    Enzyme and pathway databases

    BRENDAi 2.7.11.22. 6726.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning by differential screening of a Xenopus cDNA coding for a protein highly homologous to cdc2."
      Paris J., le Guellec R., Couturier A., le Guellec K., Omilli F., Camonis J., Macneill S., Philippe M.
      Proc. Natl. Acad. Sci. U.S.A. 88:1039-1043(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE.
      Tissue: Ovary.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Oocyte.
    3. "The cdc2-related protein p40MO15 is the catalytic subunit of a protein kinase that can activate p33cdk2 and p34cdc2."
      Poon R.Y.C., Yamashita K., Adamczewski J.P., Hunt T., Shuttleworth J.
      EMBO J. 12:3123-3132(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT THR-160.
    4. "Speedy: a novel cell cycle regulator of the G2/M transition."
      Lenormand J.-L., Dellinger R.W., Knudsen K.E., Subramani S., Donoghue D.J.
      EMBO J. 18:1869-1877(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SPDYA, ENZYME REGULATION.
      Tissue: Ovary.

    Entry informationi

    Entry nameiCDK2_XENLA
    AccessioniPrimary (citable) accession number: P23437
    Secondary accession number(s): Q3KPN7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: May 2, 2006
    Last modified: October 1, 2014
    This is version 97 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3