P23433 (E13D_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase

Gene names

Name:

SP41B

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space › extracellular matrix. Note: Stylar secretory matrix. Ref.1
Developmental stage	Maximal level of accumulation during anthesis. Ref.1
Post-translational modification	Glycosylated. Ref.1 The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Hypersensitive response Plant defense
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro plant-type hypersensitive response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32
	Chain	33 – 351	319	Glucan endo-1,3-beta-glucosidase		PRO_0000011876
Sites
	Active site	268	1	Nucleophile By similarity
	Active site	328	1	Proton donor By similarity
Amino acid modifications
	Modified residue	33	1	Pyrrolidone carboxylic acid Probable Ref.1
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	235	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P23433 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C13273CFA59A3F8E	FASTA	351	39,262
	10 20 30 40 50 60 MALWYLFNKR SLGAAVLILV GLLMCNIQIT GAQSNIGVCY GEIANNLPSE QDVINLYKAN 70 80 90 100 110 120 GIRKMRIYYP DTNIFKALNG SNIEIILEVP NQDLEALANS SIANGWVQDN IRSHFPYVKF 130 140 150 160 170 180 KYISIGNEVS PTNNGQYSQF LLHAMKNVYN ALAAAGLQDK IKVSTATYSG LLANTYPPKD 190 200 210 220 230 240 SIFREELKSF INPIIEFLAR NNLPLLANIY PYFGHIYNTV DVPLSYALFN QQETNSTGYQ 250 260 270 280 290 300 NLFDALLDSI YFAVEKAGGP NVEIIVSESG WPSEGNSAAT IENAQTYYRN LVNHVKGGAG 310 320 330 340 350 TPKKPGRIIE TYLFAMFDEN EKQGEITEKH FGLFYPNRAA KYQLNFMYSD S

References

[1]

"A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass." Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R. EMBO J. 9:3429-3436(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X54431 mRNA. Translation: CAA38303.1.
PIR	S12014.
3D structure databases
ProteinModelPortal	P23433.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13D_TOBAC

Accession

Primary (citable) accession number: P23433

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program