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P23432 (E13C_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene names
Name:SP41A
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length351 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Implicated in the defense of plants against pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedextracellular spaceextracellular matrix. Note: Stylar secretory matrix. Ref.1 Ref.2

Developmental stage

Maximal level of accumulation during anthesis. Ref.1 Ref.2

Post-translational modification

Glycosylated. Ref.1

The N-terminus is blocked.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232
Chain33 – 351319Glucan endo-1,3-beta-glucosidase
PRO_0000011875

Sites

Active site2681Nucleophile By similarity
Active site3281Proton donor By similarity

Amino acid modifications

Modified residue331Pyrrolidone carboxylic acid Probable Ref.1
Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2351N-linked (GlcNAc...) Potential
Glycosylation3371N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P23432 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: 5B8B82C3E8C71F39

FASTA35139,186
        10         20         30         40         50         60 
MALWYLFNKR SLGAAVLILV GLLMCNIQMT GAQSNIGVCY GKIANNLPSE QDVINLYKAN 

        70         80         90        100        110        120 
GIRKMRIYNS DTNIFKSLNG SNIEIILDVP NQDLEALANS SIANGWVQDN IRSHFPYVKF 

       130        140        150        160        170        180 
KYISIGNEVS PSNNGQYSQF LLHAMENVYN ALAAAGLQDK IKVTTATYSG LLANTYPPKD 

       190        200        210        220        230        240 
SIFREEFKSF INPIIEFLAR NNLPLLANIY PYFGHIYNTV DVPLSYALFN QQGTNSTGYQ 

       250        260        270        280        290        300 
NLFDALLDSI YFAVEKAGGP NVEIIVSESG WPSEGNSAAT IENAQTYYRN LVNHVKGGAG 

       310        320        330        340        350 
TPKKPGRIVE TYLFAMFDEN EKNGEVTEKH FGLFYPNRTA KYQLNFMYSD T 

« Hide

References

[1]"A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass."
Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R.
EMBO J. 9:3429-3436(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]"The expression of an abundant transmitting tract-specific endoglucanase (Sp41) is promoter-dependent and not essential for the reproductive physiology of tobacco."
Sessa G., Fluhr R.
Plant Mol. Biol. 29:969-982(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
Strain: cv. Samsun NN.
Tissue: Style.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54430 mRNA. Translation: CAA38302.1.
X81560 Genomic DNA. Translation: CAA57255.1.
PIRS12013. S62695.

3D structure databases

ProteinModelPortalP23432.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Proteomic databases

ProMEXP23432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13C_TOBAC
AccessionPrimary (citable) accession number: P23432
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries