Reviewed, UniProtKB/Swiss-Prot P23432 (E13C_TOBAC)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase

Gene names

Name:

SP41A

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	351 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space › extracellular matrix. Note: Stylar secretory matrix. Ref.1 Ref.2
Developmental stage	Maximal level of accumulation during anthesis. Ref.1 Ref.2
Post-translational modification	Glycosylated. Ref.1 The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Hypersensitive response Plant defense
Cellular component	Extracellular matrix Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro plant-type hypersensitive response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32
	Chain	33 – 351	319	Glucan endo-1,3-beta-glucosidase		PRO_0000011875
Sites
	Active site	268	1	Nucleophile By similarity
	Active site	328	1	Proton donor By similarity
Amino acid modifications
	Modified residue	33	1	Pyrrolidone carboxylic acid Probable Ref.1
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	235	1	N-linked (GlcNAc...) Potential
	Glycosylation	337	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P23432-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 5B8B82C3E8C71F39	FASTA	351	39,186
	10 20 30 40 50 60 MALWYLFNKR SLGAAVLILV GLLMCNIQMT GAQSNIGVCY GKIANNLPSE QDVINLYKAN 70 80 90 100 110 120 GIRKMRIYNS DTNIFKSLNG SNIEIILDVP NQDLEALANS SIANGWVQDN IRSHFPYVKF 130 140 150 160 170 180 KYISIGNEVS PSNNGQYSQF LLHAMENVYN ALAAAGLQDK IKVTTATYSG LLANTYPPKD 190 200 210 220 230 240 SIFREEFKSF INPIIEFLAR NNLPLLANIY PYFGHIYNTV DVPLSYALFN QQGTNSTGYQ 250 260 270 280 290 300 NLFDALLDSI YFAVEKAGGP NVEIIVSESG WPSEGNSAAT IENAQTYYRN LVNHVKGGAG 310 320 330 340 350 TPKKPGRIVE TYLFAMFDEN EKNGEVTEKH FGLFYPNRTA KYQLNFMYSD T

References

[1]	"A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass." Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R. EMBO J. 9:3429-3436(1990) [PubMed: 2120041] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
[2]	"The expression of an abundant transmitting tract-specific endoglucanase (Sp41) is promoter-dependent and not essential for the reproductive physiology of tobacco." Sessa G., Fluhr R. Plant Mol. Biol. 29:969-982(1995) [PubMed: 8555460] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE. Strain: cv. Samsun NN. Tissue: Style.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54430 mRNA. Translation: CAA38302.1. X81560 Genomic DNA. Translation: CAA57255.1.
PIR	S12013. S62695.
3D structure databases
HSSP	HSSP built from PDB template 1AQ0 based on UniProtKB P12257.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 298.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13C_TOBAC

Accession

Primary (citable) accession number: P23432

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	September 22, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)