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P23432

- E13C_TOBAC

UniProt

P23432 - E13C_TOBAC

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Protein

Glucan endo-1,3-beta-glucosidase

Gene

SP41A

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei268 – 2681NucleophileBy similarity
Active sitei328 – 3281Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. plant-type hypersensitive response Source: UniProtKB-KW
  3. response to biotic stimulus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Pathogenesis-related protein

Keywords - Biological processi

Hypersensitive response, Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene namesi
Name:SP41A
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

Secretedextracellular spaceextracellular matrix 2 Publications
Note: Stylar secretory matrix.

GO - Cellular componenti

  1. proteinaceous extracellular matrix Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Chaini33 – 351319Glucan endo-1,3-beta-glucosidasePRO_0000011875Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acid1 Publication
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi235 – 2351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi337 – 3371N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Glycosylated.1 Publication
The N-terminus is blocked.

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

ProMEXiP23432.

Expressioni

Developmental stagei

Maximal level of accumulation during anthesis.2 Publications

Structurei

3D structure databases

ProteinModelPortaliP23432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P23432-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALWYLFNKR SLGAAVLILV GLLMCNIQMT GAQSNIGVCY GKIANNLPSE
60 70 80 90 100
QDVINLYKAN GIRKMRIYNS DTNIFKSLNG SNIEIILDVP NQDLEALANS
110 120 130 140 150
SIANGWVQDN IRSHFPYVKF KYISIGNEVS PSNNGQYSQF LLHAMENVYN
160 170 180 190 200
ALAAAGLQDK IKVTTATYSG LLANTYPPKD SIFREEFKSF INPIIEFLAR
210 220 230 240 250
NNLPLLANIY PYFGHIYNTV DVPLSYALFN QQGTNSTGYQ NLFDALLDSI
260 270 280 290 300
YFAVEKAGGP NVEIIVSESG WPSEGNSAAT IENAQTYYRN LVNHVKGGAG
310 320 330 340 350
TPKKPGRIVE TYLFAMFDEN EKNGEVTEKH FGLFYPNRTA KYQLNFMYSD

T
Length:351
Mass (Da):39,186
Last modified:November 1, 1991 - v1
Checksum:i5B8B82C3E8C71F39
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54430 mRNA. Translation: CAA38302.1.
X81560 Genomic DNA. Translation: CAA57255.1.
PIRiS62695. S12013.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X54430 mRNA. Translation: CAA38302.1 .
X81560 Genomic DNA. Translation: CAA57255.1 .
PIRi S62695. S12013.

3D structure databases

ProteinModelPortali P23432.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Proteomic databases

ProMEXi P23432.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A major stylar matrix polypeptide (sp41) is a member of the pathogenesis-related proteins superclass."
    Ori N., Sessa G., Lotan T., Himmelhoch S., Fluhr R.
    EMBO J. 9:3429-3436(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE, GLYCOSYLATION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
  2. "The expression of an abundant transmitting tract-specific endoglucanase (Sp41) is promoter-dependent and not essential for the reproductive physiology of tobacco."
    Sessa G., Fluhr R.
    Plant Mol. Biol. 29:969-982(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.
    Strain: cv. Samsun NN.
    Tissue: Style.

Entry informationi

Entry nameiE13C_TOBAC
AccessioniPrimary (citable) accession number: P23432
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3