ID   E13B_NICPL              Reviewed;         365 AA.
AC   P23431;
DT   01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 1.
DT   22-FEB-2023, entry version 83.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase, basic;
DE   Flags: Precursor;
GN   Name=GN2;
OS   Nicotiana plumbaginifolia (Leadwort-leaved tobacco) (Tex-Mex tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4092;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2123542; DOI=10.1093/nar/18.22.6685;
RA   Gheysen G.D., Inze D., Soetaert P., van Montagu M., Castresana C.;
RT   "Sequence of a Nicotiana plumbaginifolia beta(1,3)-glucanase gene encoding
RT   a vacuolar isoform.";
RL   Nucleic Acids Res. 18:6685-6685(1990).
CC   -!- FUNCTION: Implicated in the defense of plants against pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SUBCELLULAR LOCATION: Vacuole.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; X54742; CAA38540.1; -; Genomic_DNA.
DR   PIR; S13594; S13594.
DR   AlphaFoldDB; P23431; -.
DR   SMR; P23431; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   GlyCosmos; P23431; 1 site, No reported glycans.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227:SF251; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE B; 1.
DR   PANTHER; PTHR32227; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Glycosidase; Hydrolase; Plant defense; Signal; Vacuole.
FT   SIGNAL          1..32
FT   CHAIN           33..348
FT                   /note="Glucan endo-1,3-beta-glucosidase, basic vacuolar
FT                   isoform"
FT                   /id="PRO_0000011867"
FT   PROPEP          349..365
FT                   /note="Removed in mature form"
FT                   /evidence="ECO:0000250"
FT                   /id="PRO_0000011868"
FT   ACT_SITE        128
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        273
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   CARBOHYD        356
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   365 AA;  39984 MW;  C744FD90E1D9C505 CRC64;
     MSTLHKHNTP QMAAITLLGL LLVASSIEIA GAESIGVCYG MLGNNLPNHW EVIQLYKSRN
     IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI
     KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS
     QGSFRNDARW FVDPIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG
     SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLKNLIQHA
     KEGSPRKPRP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS GSVETNATAS
     LISEI
//