Reviewed, UniProtKB/Swiss-Prot P23431 (E13B_NICPL)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase, basic

Gene names

Name:

GN2

Organism

Nicotiana plumbaginifolia (Leadwort-leaved tobacco)

Taxonomic identifier

4092 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 32	32
	Chain	33 – 348	316	Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform		PRO_0000011867
	Propeptide	349 – 365	17	Removed in mature form By similarity		PRO_0000011868
Sites
	Active site	273	1	Nucleophile By similarity
	Active site	329	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	356	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P23431-1 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: C744FD90E1D9C505	FASTA	365	39,984
	10 20 30 40 50 60 MSTLHKHNTP QMAAITLLGL LLVASSIEIA GAESIGVCYG MLGNNLPNHW EVIQLYKSRN 70 80 90 100 110 120 IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI 130 140 150 160 170 180 KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS 190 200 210 220 230 240 QGSFRNDARW FVDPIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG 250 260 270 280 290 300 SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLKNLIQHA 310 320 330 340 350 360 KEGSPRKPRP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS GSVETNATAS LISEI

References

[1]	"Sequence of a Nicotiana plumbaginifolia beta(1,3)-glucanase gene encoding a vacuolar isoform." Gheysen G.D., Inze D., Soetaert P., van Montagu M., Castresana C. Nucleic Acids Res. 18:6685-6685(1990) [PubMed: 2123542] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54742 Genomic DNA. Translation: CAA38540.1.
PIR	S13594.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 257040.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_NICPL

Accession

Primary (citable) accession number: P23431

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)