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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi127Zinc1
Metal bindingi130Zinc1
Metal bindingi144Zinc1
Metal bindingi147Zinc1
Binding sitei300ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Synonyms:metS
Ordered Locus Names:TTHA1298
OrganismiThermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
Taxonomic identifieri300852 [NCBI]
Taxonomic lineageiBacteriaDeinococcus-ThermusDeinococciThermalesThermaceaeThermus
Proteomesi
  • UP000000532 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1641341.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392581 – 618Methionine--tRNA ligaseAdd BLAST618

Proteomic databases

PRIDEiP23395.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi300852.TTHA1298.

Structurei

Secondary structure

1618
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Helixi20 – 38Combined sources19
Beta strandi42 – 50Combined sources9
Helixi54 – 63Combined sources10
Helixi67 – 84Combined sources18
Beta strandi90 – 94Combined sources5
Helixi98 – 113Combined sources16
Beta strandi117 – 127Combined sources11
Turni128 – 131Combined sources4
Beta strandi132 – 134Combined sources3
Turni136 – 138Combined sources3
Turni145 – 147Combined sources3
Beta strandi152 – 161Combined sources10
Helixi163 – 166Combined sources4
Helixi167 – 175Combined sources9
Beta strandi180 – 183Combined sources4
Helixi184 – 194Combined sources11
Beta strandi204 – 206Combined sources3
Turni207 – 209Combined sources3
Beta strandi219 – 224Combined sources6
Helixi226 – 231Combined sources6
Helixi233 – 236Combined sources4
Turni237 – 242Combined sources6
Helixi244 – 249Combined sources6
Helixi250 – 252Combined sources3
Beta strandi253 – 258Combined sources6
Helixi259 – 261Combined sources3
Helixi262 – 266Combined sources5
Helixi268 – 276Combined sources9
Beta strandi282 – 287Combined sources6
Turni300 – 302Combined sources3
Helixi308 – 315Combined sources8
Helixi317 – 327Combined sources11
Helixi338 – 348Combined sources11
Helixi349 – 353Combined sources5
Helixi354 – 367Combined sources14
Helixi379 – 386Combined sources8
Helixi387 – 396Combined sources10
Helixi400 – 421Combined sources22
Helixi423 – 426Combined sources4
Turni427 – 429Combined sources3
Helixi431 – 452Combined sources22
Turni453 – 455Combined sources3
Helixi457 – 466Combined sources10
Helixi475 – 479Combined sources5
Beta strandi480 – 482Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8HX-ray2.00A1-500[»]
1WOYX-ray2.00A1-500[»]
2D54X-ray2.00A1-502[»]
2D5BX-ray1.80A1-500[»]
3VU8X-ray2.20A1-502[»]
ProteinModelPortaliP23395.
SMRiP23395.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP23395.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini518 – 618tRNA-bindingAdd BLAST101

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi12 – 22"HIGH" regionAdd BLAST11
Motifi297 – 301"KMSKS" region5

Sequence similaritiesi

Contains 1 tRNA-binding domain.Curated

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.
PhylomeDBiP23395.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23395-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKVFYVTTP IYYVNAEPHL GHAYTTVVAD FLARWHRLDG YRTFFLTGTD
60 70 80 90 100
EHGETVYRAA QAAGEDPKAF VDRVSGRFKR AWDLLGIAYD DFIRTTEERH
110 120 130 140 150
KKVVQLVLKK VYEAGDIYYG EYEGLYCVSC ERFYTEKELV EGLCPIHGRP
160 170 180 190 200
VERRKEGNYF FRMEKYRPWL QEYIQENPDL IRPEGYRNEV LAMLAEPIGD
210 220 230 240 250
LSISRPKSRV PWGIPLPWDE NHVTYVWFDA LLNYVSALDY PEGEAYRTFW
260 270 280 290 300
PHAWHLIGKD ILKPHAVFWP TMLKAAGIPM YRHLNVGGFL LGPDGRKMSK
310 320 330 340 350
TLGNVVDPFA LLEKYGRDAL RYYLLREIPY GQDTPVSEEA LRTRYEADLA
360 370 380 390 400
DDLGNLVQRT RAMLFRFAEG RIPEPVAGEE LAEGTGLAGR LRPLVRELKF
410 420 430 440 450
HVALEEAMAY VKALNRYINE KKPWELFKKE PEEARAVLYR VVEGLRIASI
460 470 480 490 500
LLTPAMPDKM AELRRALGLK EEVRLEEAER WGLAEPRPIP EEAPVLFPKK
510 520 530 540 550
EAKVEAKPKE EAWIGIEDFA KVELRVAEVL AAEKHPNADR LLVLRLSLGN
560 570 580 590 600
EERTVVSGIA KWYRPEELVG KKVVLVANLK PAKLRGIESQ GMILAAQEGE
610
ALALVTVEGE VPPGAVVK
Length:618
Mass (Da):70,693
Last modified:March 29, 2005 - v2
Checksum:i920A449052C62558
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti615 – 618AVVK → RW in AAA27510 (PubMed:1993699).Curated4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64273 Genomic DNA. Translation: AAA27510.1.
AP008226 Genomic DNA. Translation: BAD71121.1.
RefSeqiWP_011228577.1. NC_006461.1.
YP_144564.1. NC_006461.1.

Genome annotation databases

EnsemblBacteriaiBAD71121; BAD71121; BAD71121.
GeneIDi3169329.
KEGGittj:TTHA1298.
PATRICi23957557. VBITheThe93045_1276.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M64273 Genomic DNA. Translation: AAA27510.1.
AP008226 Genomic DNA. Translation: BAD71121.1.
RefSeqiWP_011228577.1. NC_006461.1.
YP_144564.1. NC_006461.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A8HX-ray2.00A1-500[»]
1WOYX-ray2.00A1-500[»]
2D54X-ray2.00A1-502[»]
2D5BX-ray1.80A1-500[»]
3VU8X-ray2.20A1-502[»]
ProteinModelPortaliP23395.
SMRiP23395.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi300852.TTHA1298.

Chemistry databases

ChEMBLiCHEMBL1641341.

Proteomic databases

PRIDEiP23395.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAD71121; BAD71121; BAD71121.
GeneIDi3169329.
KEGGittj:TTHA1298.
PATRICi23957557. VBITheThe93045_1276.

Phylogenomic databases

eggNOGiENOG4105CKH. Bacteria.
COG0073. LUCA.
COG0143. LUCA.
HOGENOMiHOG000200401.
KOiK01874.
OMAiHTFYVWF.
PhylomeDBiP23395.

Miscellaneous databases

EvolutionaryTraceiP23395.
PROiP23395.

Family and domain databases

CDDicd00814. MetRS_core. 1 hit.
cd02800. tRNA_bind_EcMetRS_like. 1 hit.
Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_01228. Met_tRNA_synth_type2. 1 hit.
InterProiIPR002300. aa-tRNA-synth_Ia.
IPR004495. Met-tRNA-synth_bsu_C.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR033911. MetRS_core.
IPR012340. NA-bd_OB-fold.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002547. tRNA-bd_dom.
IPR009080. tRNAsynth_Ia_anticodon-bd.
[Graphical view]
PfamiPF00133. tRNA-synt_1. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.
TIGR00399. metG_C_term. 1 hit.
PROSITEiPS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYM_THET8
AccessioniPrimary (citable) accession number: P23395
Secondary accession number(s): Q5SIR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: March 29, 2005
Last modified: November 30, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.