P23388 (PTFAX_RHOCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multiphosphoryl transfer protein
Short name=MTP

Including the following 3 domains:

1. Phosphoenolpyruvate-protein phosphotransferase
   EC=2.7.3.9
   Alternative name(s):
   Phosphotransferase system enzyme I
2. Phosphocarrier protein HPr
   Short name=Protein H
3. Fructose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   EIII-Fru
   PTS system fructose-specific EIIA component

Gene names

Name:

fruB(HI)

Organism

Rhodobacter capsulatus (Rhodopseudomonas capsulata)

Taxonomic identifier

1061 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Rhodobacter

Protein attributes

Sequence length	827 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport By similarity.
Catalytic activity	Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)-phospho-L-histidine. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cytoplasm Potential.
Domain	The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Belongs to the PEP-utilizing enzyme family. Contains 1 HPr domain. Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW phosphoenolpyruvate-protein phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 827	827	Multiphosphoryl transfer protein		PRO_0000186512
Regions
	Domain	2 – 142	141	PTS EIIA type-2
	Domain	157 – 245	89	HPr
	Region	270 – 827	558	PTS EI
Sites
	Active site	62	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity
	Active site	171	1	Pros-phosphohistidine intermediate; for HPr activity By similarity
	Active site	457	1	Tele-phosphohistidine intermediate; for PTS EI activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P23388 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: 82E536754187696B	FASTA	827	86,394
	10 20 30 40 50 60 MIPLTSELVA IGKTATDKAD AIAQAVDLLT AAGKIDPRYG QSMMGREAVA NTFLGNGIAI 70 80 90 100 110 120 PHGLPQDRDL IHDTAIAVVQ LPAGVEWAPG DTARLVVAIA AKSDEHLQVL SNLTDVLGDE 130 140 150 160 170 180 AEAERLATTL DAAVIVARLT GAAAPVAAPA ETPADFAQGI DVVVTGAHGL HARPATTLVD 190 200 210 220 230 240 LAKGFAAEIR IRNGAKVANG KSLISLLNLG AAQGAALRIS AEGADATAAL AAIAAAFEAG 250 260 270 280 290 300 LEDEEDTGAA APEAATPGLT GAGASMASYE GRTLVGISSS PGYALAPVFR FARDEVVFDT 310 320 330 340 350 360 DAADAAFETD RLDTALQTAW HELEELHDEV WKTSGPARAA IFRAHQEFLH DPEMVAEAKA 370 380 390 400 410 420 LIGQGRSAGF AWHRVFSDRA DMLGAMKDAV LSGRAIDLRD AGQRVLQHLG RVRTGETHLP 430 440 450 460 470 480 TAPCILLADD LTPSDTARLD PALVRGLATA QGGPTSHTSI IARALDIPAV AGVGPRLLDL 490 500 510 520 530 540 ATGTPVLLDG GAGVIVVAPT EADKARAETA MAALTAQREL EARERYKPAL TVDGARVEVV 550 560 570 580 590 600 ANISDVAEAI ASVEAGAEGV GLLRTEFLFV NREAPPGEDE QLAIYAAMLS ALNGLPIIIR 610 620 630 640 650 660 TLDVGGDKEI PYLRMPVEQN PFLGERGIRF CLSHEDLFRT QLRAIYRASA GGQVRIMFPM 670 680 690 700 710 720 IAMIEELETA RRIAEEVRLE VGAAPVEIGI MIEIPSAVMM APELAKRVDF FSIGTNDLTQ 730 740 750 760 770 780 YALAMDRMHP VLAKQADGLH PAVLRLIDST VRAAEAARIW VGACGGIAGD PVGAAVLSGL 790 800 810 820 GVRELSVSIP AVAGIKAQLR HSAMAENRDL ARRALACTTA AEVRGLK

References

[1]	"Structure and evolution of a multidomain multiphosphoryl transfer protein. Nucleotide sequence of the fruB(HI) gene in Rhodobacter capsulatus and comparisons with homologous genes from other organisms." Wu L.-F., Tomich J.M., Saier M.H. Jr. J. Mol. Biol. 213:687-703(1990) [PubMed: 2193161] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: DSM 938 / 37b4.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53150 Genomic DNA. Translation: CAA37301.1.
PIR	S10639.
3D structure databases
ProteinModelPortal	P23388.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR008279. PEP-util_enz_mobile_dom. IPR018274. PEP_util_AS. IPR000121. PEP_util_C. IPR023151. PEP_util_CS. IPR006318. PEP_util_enz. IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. IPR008731. PTS_PEP_utilis_N. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.40.930.10. PTS_EIIA_2. 1 hit. G3DSA:3.30.1340.10. PTS_HPr_protein. 1 hit. G3DSA:1.10.274.10. PTS_PEP_utilis_N. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
Pfam	PF05524. PEP-utilisers_N. 1 hit. PF00391. PEP-utilizers. 1 hit. PF02896. PEP-utilizers_C. 1 hit. PF00381. PTS-HPr. 1 hit. PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
PRINTS	PR00107. PHOSPHOCPHPR. PR01736. PHPHTRNFRASE.
SUPFAM	SSF55594. HPr_protein. 1 hit. SSF47831. PEP-utilisers_N. 1 hit. SSF52009. PEP_mobile. 1 hit. SSF55804. PTrfase/Anion_transptr. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01003. PTS_HPr_family. 1 hit. TIGR01417. PTS_I_fam. 1 hit.
PROSITE	PS00742. PEP_ENZYMES_2. 1 hit. PS00370. PEP_ENZYMES_PHOS_SITE. 1 hit. PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51350. PTS_HPR_DOM. 1 hit. PS00369. PTS_HPR_HIS. 1 hit. PS00589. PTS_HPR_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFAX_RHOCA

Accession

Primary (citable) accession number: P23388

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	October 19, 2011
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program