Skip Header

 
Contribute Send feedback
Read comments (1) or add your own

Reviewed, UniProtKB/Swiss-Prot P23385 (GRM1_RAT)

Last modified June 16, 2009. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Metabotropic glutamate receptor 1
      Short name=mGluR1
Gene names
Name: Grm1
Synonyms: Gprc1a, Mglur1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Hide | Top

Protein attributes

Sequence length1199 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Receptor for glutamate. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system. May participate in the central action of glutamate in the CNS, such as long-term potentiation in the hippocampus and long-term depression in the cerebellum.

Subunit structure

Homodimer; disulfide-linked. The PPXXF motif binds HOMER1, HOMER2 and HOMER3. Interacts with RYR1, RYR2, ITPR1, SHANK1 and SHANK3 By similarity. Interacts with SIAH1 and GRASP.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Tissue specificity

Predominantly expressed in cerebellar Purkinje cells, CA2-CA3 pyramidal cells of the hippocampus, and mitral and tufted cells of the olfactory bulb.

Miscellaneous

Activated by quisqualate > glutamate > ibotenate > trans-1- aminocyclopentyl-1,3-dicarboxylate; inhibited by 2-amino-3-phosphonopropionate.

Sequence similarities

Belongs to the G-protein coupled receptor 3 family.

Hide | Top

Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   Coding sequence diversityAlternative splicing
   DomainSignal
Transmembrane
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological processactivation of phospholipase C activity by metabotropic glutamate receptor signaling pathway

Traceable author statement. Source: UniProtKB

calcium-mediated signaling

Inferred from direct assay. Source: RGD

elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating)

Traceable author statement. Source: UniProtKB

membrane depolarization

Traceable author statement. Source: UniProtKB

regulation of glutamate secretion

Traceable author statement. Source: UniProtKB

regulation of synaptic transmission, glutamatergic

Inferred from direct assay. Source: UniProtKB

sensory perception of pain

Inferred from expression pattern. Source: RGD

synaptic transmission, GABAergic

Traceable author statement. Source: UniProtKB

   Cellular componentaxon

Inferred from direct assay. Source: UniProtKB

dendritic spine

Inferred from direct assay. Source: RGD

integral to plasma membrane

Traceable author statement. Source: RGD

postsynaptic density

Inferred from direct assay. Source: RGD

presynaptic membrane

Inferred from direct assay. Source: UniProtKB

   Molecular functionG-protein coupled receptor activity

Traceable author statement. Source: RGD

PLC activating metabotropic glutamate receptor activity

Traceable author statement. Source: UniProtKB

Complete GO annotation...

Hide | Top

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1A (identifier: P23385-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 1B (identifier: P23385-2)

The sequence of this isoform differs from the canonical sequence as follows:
     887-906: NSNGKSVSWSEPGGRQAPKG → KKRQPEFSPSSQCPSAHAQL
     907-1199: Missing.
Note: C-terminally truncated forms of isoform 1A.
Isoform 1C (identifier: P23385-3)

The sequence of this isoform differs from the canonical sequence as follows:
     888-897: SNGKSVSWSE → FALDRQNTVY
     898-1199: Missing.
Note: C-terminally truncated forms of isoform 1A.

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Chain19 – 11991181Metabotropic glutamate receptor 1
PRO_0000012924

Regions

Topological domain19 – 592574Extracellular Potential
Transmembrane593 – 615231 Potential
Topological domain616 – 62914Cytoplasmic Potential
Transmembrane630 – 650212 Potential
Topological domain651 – 66111Extracellular Potential
Transmembrane662 – 680193 Potential
Topological domain681 – 70626Cytoplasmic Potential
Transmembrane707 – 727214 Potential
Topological domain728 – 75023Extracellular Potential
Transmembrane751 – 772225 Potential
Topological domain773 – 78513Cytoplasmic Potential
Transmembrane786 – 808236 Potential
Topological domain809 – 8146Extracellular Potential
Transmembrane815 – 840267 Potential
Topological domain841 – 1199359Cytoplasmic Potential
Compositional bias1014 – 103421Gln/Pro-rich
Compositional bias1074 – 10807Gln/Pro-rich
Compositional bias1126 – 113510Asp/Glu-rich (acidic)
Compositional bias1140 – 119960Ser-rich

Amino acid modifications

Modified residue6721Phosphotyrosine By similarity
Modified residue11191Phosphotyrosine By similarity
Glycosylation981N-linked (GlcNAc...) Potential
Glycosylation2231N-linked (GlcNAc...) Potential
Glycosylation3971N-linked (GlcNAc...) Potential
Glycosylation5151N-linked (GlcNAc...) Potential
Disulfide bond140Interchain By similarity

Natural variations

Alternative sequence887 – 90620NSNGK…QAPKG → KKRQPEFSPSSQCPSAHAQL in isoform 1B.
VSP_002026
Alternative sequence888 – 89710SNGKSVSWSE → FALDRQNTVY in isoform 1C.
VSP_002028
Alternative sequence898 – 1199302Missing in isoform 1C.
VSP_002029
Alternative sequence907 – 1199293Missing in isoform 1B.
VSP_002027

Secondary structure

1 ................................................................................. 1199
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
Isoform 1A [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: EEE5A04C50694B9F

FASTA1,199133,236
        10         20         30         40         50         60 
MVRLLLIFFP MIFLEMSILP RMPDRKVLLA GASSQRSVAR MDGDVIIGAL FSVHHQPPAE 

        70         80         90        100        110        120 
KVPERKCGEI REQYGIQRVE AMFHTLDKIN ADPVLLPNIT LGSEIRDSCW HSSVALEQSI 

       130        140        150        160        170        180 
EFIRDSLISI RDEKDGLNRC LPDGQTLPPG RTKKPIAGVI GPGSSSVAIQ VQNLLQLFDI 

       190        200        210        220        230        240 
PQIAYSATSI DLSDKTLYKY FLRVVPSDTL QARAMLDIVK RYNWTYVSAV HTEGNYGESG 

       250        260        270        280        290        300 
MDAFKELAAQ EGLCIAHSDK IYSNAGEKSF DRLLRKLRER LPKARVVVCF CEGMTVRGLL 

       310        320        330        340        350        360 
SAMRRLGVVG EFSLIGSDGW ADRDEVIEGY EVEANGGITI KLQSPEVRSF DDYFLKLRLD 

       370        380        390        400        410        420 
TNTRNPWFPE FWQHRFQCRL PGHLLENPNF KKVCTGNESL EENYVQDSKM GFVINAIYAM 

       430        440        450        460        470        480 
AHGLQNMHHA LCPGHVGLCD AMKPIDGRKL LDFLIKSSFV GVSGEEVWFD EKGDAPGRYD 

       490        500        510        520        530        540 
IMNLQYTEAN RYDYVHVGTW HEGVLNIDDY KIQMNKSGMV RSVCSEPCLK GQIKVIRKGE 

       550        560        570        580        590        600 
VSCCWICTAC KENEFVQDEF TCRACDLGWW PNAELTGCEP IPVRYLEWSD IESIIAIAFS 

       610        620        630        640        650        660 
CLGILVTLFV TLIFVLYRDT PVVKSSSREL CYIILAGIFL GYVCPFTLIA KPTTTSCYLQ 

       670        680        690        700        710        720 
RLLVGLSSAM CYSALVTKTN RIARILAGSK KKICTRKPRF MSAWAQVIIA SILISVQLTL 

       730        740        750        760        770        780 
VVTLIIMEPP MPILSYPSIK EVYLICNTSN LGVVAPVGYN GLLIMSCTYY AFKTRNVPAN 

       790        800        810        820        830        840 
FNEAKYIAFT MYTTCIIWLA FVPIYFGSNY KIITTCFAVS LSVTVALGCM FTPKMYIIIA 

       850        860        870        880        890        900 
KPERNVRSAF TTSDVVRMHV GDGKLPCRSN TFLNIFRRKK PGAGNANSNG KSVSWSEPGG 

       910        920        930        940        950        960 
RQAPKGQHVW QRLSVHVKTN ETACNQTAVI KPLTKSYQGS GKSLTFSDAS TKTLYNVEEE 

       970        980        990       1000       1010       1020 
DNTPSAHFSP PSSPSMVVHR RGPPVATTPP LPPHLTAEET PLFLADSVIP KGLPPPLPQQ 

      1030       1040       1050       1060       1070       1080 
QPQQPPPQQP PQQPKSLMDQ LQGVVTNFGS GIPDFHAVLA GPGTPGNSLR SLYPPPPPPQ 

      1090       1100       1110       1120       1130       1140 
HLQMLPLHLS TFQEESISPP GEDIDDDSER FKLLQEFVYE REGNTEEDEL EEEEDLPTAS 

      1150       1160       1170       1180       1190 
KLTPEDSPAL TPPSPFRDSV ASGSSVPSSP VSESVLCTPP NVTYASVILR DYKQSSSTL

« Hide

Isoform 1B.

Checksum: 93201A86AD366DB3
Show »

FASTA906101,638
Isoform 1C.

Checksum: 6F1E809C2AC521B9
Show »

FASTA897100,752

Hide | Top

References

[1]"Sequence and expression of a metabotropic glutamate receptor."
Masu M., Tanabe Y., Tsuchida K., Shigemoto R., Nakanishi S.
Nature 349:760-765(1991) [PubMed: 1847995] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Cloning, expression, and gene structure of a G protein-coupled glutamate receptor from rat brain."
Houamed K.M., Kuijper J.L., Gilbert T.L., Haldeman B.A., O'Hara P.J., Mulvihill E.R., Almers W., Hagen F.S.
Science 252:1318-1321(1991) [PubMed: 1656524] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[3]"A family of metabotropic glutamate receptors."
Tanabe Y., Masu M., Ishii T., Shigemoto R., Nakanishi S.
Neuron 8:169-179(1992) [PubMed: 1309649] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM 1B).
Tissue: Brain.
[4]"Alternative splicing generates metabotropic glutamate receptors inducing different patterns of calcium release in Xenopus oocytes."
Pin J.-P., Waeber C., Prezeau L., Bockaert J., Heinemann S.F.
Proc. Natl. Acad. Sci. U.S.A. 89:10331-10335(1992) [PubMed: 1438218] [Abstract]
Cited for: ALTERNATIVE SPLICING (ISOFORM 1C).
Tissue: Brain.
[5]"Competitive interaction of seven in absentia homolog-1A and Ca2+/calmodulin with the cytoplasmic tail of group 1 metabotropic glutamate receptors."
Ishikawa K., Nash S.R., Nishimune A., Neki A., Kaneko S., Nakanishi S.
Genes Cells 4:381-390(1999) [PubMed: 10469171] [Abstract]
Cited for: INTERACTION WITH SIAH1.
[6]"Tamalin, a PDZ domain-containing protein, links a protein complex formation of group 1 metabotropic glutamate receptors and the guanine nucleotide exchange factor cytohesins."
Kitano J., Kimura K., Yamazaki Y., Soda T., Shigemoto R., Nakajima Y., Nakanishi S.
J. Neurosci. 22:1280-1289(2002) [PubMed: 11850456] [Abstract]
Cited for: INTERACTION WITH GRASP.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

X57569 mRNA. Translation: CAA40799.1.
M61099 mRNA. Translation: AAA19497.1.
S48085 mRNA. Translation: AAB24138.1.
IPIIPI00210260.
IPI00231006.
IPI00231007.
PIRA41939.
RefSeqNP_001107802.1.
NP_058707.1.
UniGeneRn.87787

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1EWKX-ray2.20A/B33-522[»]
1EWTX-ray3.70A/B33-522[»]
1EWVX-ray4.00A/B33-522[»]
1ISRX-ray4.00A33-522[»]
1ISSX-ray3.30A/B33-522[»]
ModBaseSearch...

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP23385.

Genome annotation databases

EnsemblENSRNOG00000014290. Rattus norvegicus. [Contig view]
GeneID24414.
KEGGrno:24414.

Organism-specific databases

RGD2742. Grm1.

Phylogenomic databases

HOVERGENP23385.

Gene expression databases

ArrayExpressP23385.
GermOnlineENSRNOG00000014290. Rattus norvegicus.

Family and domain databases

InterProIPR001828. ANF_lig_bd_rcpt.
IPR000337. GPCR_3.
IPR017978. GPCR_3_C.
IPR017979. GPCR_3_CS.
IPR000162. GPCR_3_mtglu_rcpt.
IPR001256. GPCR_3_mtglu_rcpt_1.
IPR017971. GPCR_3_mtglu_rcpt_1/5.
IPR011500. GPCR_3_NCR.
IPR019588. Metabotropic_Glu_rcpt_Homer-bd.
[Graphical view]
PANTHERPTHR11336:SF11. Mtglu_rcpt_5. 1 hit.
PfamPF00003. 7tm_3. 1 hit.
PF01094. ANF_receptor. 1 hit.
PF10606. GluR_Homer-bdg. 1 hit.
PF07562. NCD3G. 1 hit.
[Graphical view]
PRINTSPR00248. GPCRMGR.
PR01051. MTABOTROPC1R.
PR00593. MTABOTROPICR.
PROSITEPS00979. G_PROTEIN_RECEP_F3_1. 1 hit.
PS00980. G_PROTEIN_RECEP_F3_2. 1 hit.
PS00981. G_PROTEIN_RECEP_F3_3. 1 hit.
PS50259. G_PROTEIN_RECEP_F3_4. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio603247.
PMAP-CutDBP23385.

Hide | Top

Entry information

Entry nameGRM1_RAT
AccessionPrimary (citable) accession number: P23385
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: June 16, 2009
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents