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P23384 (NPRE_BACCL) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bacillolysin
EC=3.4.24.28
Alternative name(s):
Thermostable neutral protease
Gene names
Name:npr
OrganismBacillus caldolyticus
Taxonomic identifier1394 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit By similarity.

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

Biophysicochemical properties

Temperature dependence:

Thermostable.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionmetalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525
Propeptide26 – 225200Activation peptide
PRO_0000028600
Chain226 – 544319Bacillolysin
PRO_0000028601

Sites

Active site3711 By similarity
Active site4591Proton donor By similarity
Metal binding2851Calcium 1 By similarity
Metal binding2871Calcium 1 By similarity
Metal binding2891Calcium 1; via carbonyl oxygen By similarity
Metal binding3661Calcium 2 By similarity
Metal binding3701Zinc; catalytic By similarity
Metal binding3741Zinc; catalytic By similarity
Metal binding3941Zinc; catalytic By similarity
Metal binding4051Calcium 2 By similarity
Metal binding4051Calcium 3 By similarity
Metal binding4111Calcium 3; via carbonyl oxygen By similarity
Metal binding4131Calcium 2 By similarity
Metal binding4131Calcium 3 By similarity
Metal binding4151Calcium 2; via carbonyl oxygen By similarity
Metal binding4181Calcium 2 By similarity
Metal binding4181Calcium 3 By similarity
Metal binding4211Calcium 4; via carbonyl oxygen By similarity
Metal binding4221Calcium 4 By similarity
Metal binding4251Calcium 4; via carbonyl oxygen By similarity
Metal binding4281Calcium 4 By similarity

Sequences

Sequence LengthMass (Da)Tools
P23384 [UniParc].

Last modified November 1, 1991. Version 1.
Checksum: C551E67ACCA356FD

FASTA54459,413
        10         20         30         40         50         60 
MNKRAMLGAI GLAFGLMAWP FGASAKGKSM VWNEQWKTPS FVSGSLLGRC SQELVYRYLD 

        70         80         90        100        110        120 
QEKNTFQLGG QARERLSLIG NKLDELGHTV MRFEQAIAAS LCMGAVLVAH VNDGELSSLS 

       130        140        150        160        170        180 
GTLIPNLDKR TLKTEAAISI QQAEMIAKQD VADRVTKERP AAEEGKPTRL VIYPDEETPR 

       190        200        210        220        230        240 
LAYEVNVRFL TPVPGNWIYM IDAADGKVLN KWNQMDEAKP GGAQPVAGTS TVGVGRGVLG 

       250        260        270        280        290        300 
DQKYINTTYS SYYGYYYLQD NTRGSGIFTY DGRNRTVLPG SLWADGDNQF FASYDAAAVD 

       310        320        330        340        350        360 
AHYYAGVVYD YYKNVHGRLS YDGSNAAIRS TVHYGRGYNN AFWNGSQMVY GDGDGQTFLP 

       370        380        390        400        410        420 
FSGGIDVVGH ELTHAVTDYT AGLVYQNESG AINEAMSDIF GTLVEFYANR NPDWEIGEDI 

       430        440        450        460        470        480 
YTPGVAGDAL RSMSDPAKYG DPDHYSKRYT GTQDNGGVHT NSGIINKAAY LLSQGGVHYG 

       490        500        510        520        530        540 
VSVTGIGRDK MGKIFYRALV YYLTPTSNFS QLRAACVQAA ADLYGSTSQE VNSVKQAFNA 


VGVY

« Hide

References

[1]"A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product."
van den Burg B., Enequist H.G., van de Haar M.E., Eijsink V.G.H., Stulp B.K., Venema G.
J. Bacteriol. 173:4107-4115(1991) [PubMed: 1905714] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M63575 Genomic DNA. Translation: AAA22623.1.
PIRA42464.

3D structure databases

ProteinModelPortalP23384.
SMRP23384. Positions 226-543.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR011096. FTP_domain.
IPR005075. Pept_M4_propep_PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
Gene3DG3DSA:3.10.170.10. Peptidase_M4. 1 hit.
G3DSA:1.10.390.10. Peptidase_M4/M36. 1 hit.
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPRE_BACCL
AccessionPrimary (citable) accession number: P23384
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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