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P23384

- NPRE_BACCL

UniProt

P23384 - NPRE_BACCL

Protein

Bacillolysin

Gene

npr

Organism
Bacillus caldolyticus
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 Nov 1991)
      Previous versions | rss
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    Functioni

    Extracellular zinc metalloprotease.

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.By similarity
    Binds 1 zinc ion per subunit.By similarity

    Temperature dependencei

    Thermostable.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi285 – 2851Calcium 1By similarity
    Metal bindingi287 – 2871Calcium 1By similarity
    Metal bindingi289 – 2891Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi366 – 3661Calcium 2By similarity
    Metal bindingi370 – 3701Zinc; catalyticPROSITE-ProRule annotation
    Active sitei371 – 3711PROSITE-ProRule annotation
    Metal bindingi374 – 3741Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi394 – 3941Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi405 – 4051Calcium 2By similarity
    Metal bindingi405 – 4051Calcium 3By similarity
    Metal bindingi411 – 4111Calcium 3; via carbonyl oxygenBy similarity
    Metal bindingi413 – 4131Calcium 2By similarity
    Metal bindingi413 – 4131Calcium 3By similarity
    Metal bindingi415 – 4151Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi418 – 4181Calcium 2By similarity
    Metal bindingi418 – 4181Calcium 3By similarity
    Metal bindingi421 – 4211Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi422 – 4221Calcium 4By similarity
    Metal bindingi425 – 4251Calcium 4; via carbonyl oxygenBy similarity
    Metal bindingi428 – 4281Calcium 4By similarity
    Active sitei459 – 4591Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    Thermostable neutral protease
    Gene namesi
    Name:npr
    OrganismiBacillus caldolyticus
    Taxonomic identifieri1394 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillusGeobacillus thermoleovorans group

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2525Add
    BLAST
    Propeptidei26 – 225200Activation peptidePRO_0000028600Add
    BLAST
    Chaini226 – 544319BacillolysinPRO_0000028601Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP23384.
    SMRiP23384. Positions 226-543.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P23384-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNKRAMLGAI GLAFGLMAWP FGASAKGKSM VWNEQWKTPS FVSGSLLGRC    50
    SQELVYRYLD QEKNTFQLGG QARERLSLIG NKLDELGHTV MRFEQAIAAS 100
    LCMGAVLVAH VNDGELSSLS GTLIPNLDKR TLKTEAAISI QQAEMIAKQD 150
    VADRVTKERP AAEEGKPTRL VIYPDEETPR LAYEVNVRFL TPVPGNWIYM 200
    IDAADGKVLN KWNQMDEAKP GGAQPVAGTS TVGVGRGVLG DQKYINTTYS 250
    SYYGYYYLQD NTRGSGIFTY DGRNRTVLPG SLWADGDNQF FASYDAAAVD 300
    AHYYAGVVYD YYKNVHGRLS YDGSNAAIRS TVHYGRGYNN AFWNGSQMVY 350
    GDGDGQTFLP FSGGIDVVGH ELTHAVTDYT AGLVYQNESG AINEAMSDIF 400
    GTLVEFYANR NPDWEIGEDI YTPGVAGDAL RSMSDPAKYG DPDHYSKRYT 450
    GTQDNGGVHT NSGIINKAAY LLSQGGVHYG VSVTGIGRDK MGKIFYRALV 500
    YYLTPTSNFS QLRAACVQAA ADLYGSTSQE VNSVKQAFNA VGVY 544
    Length:544
    Mass (Da):59,413
    Last modified:November 1, 1991 - v1
    Checksum:iC551E67ACCA356FD
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63575 Genomic DNA. Translation: AAA22623.1.
    PIRiA42464.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63575 Genomic DNA. Translation: AAA22623.1 .
    PIRi A42464.

    3D structure databases

    ProteinModelPortali P23384.
    SMRi P23384. Positions 226-543.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A highly thermostable neutral protease from Bacillus caldolyticus: cloning and expression of the gene in Bacillus subtilis and characterization of the gene product."
      van den Burg B., Enequist H.G., van de Haar M.E., Eijsink V.G.H., Stulp B.K., Venema G.
      J. Bacteriol. 173:4107-4115(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiNPRE_BACCL
    AccessioniPrimary (citable) accession number: P23384
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3