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Protein

V-type proton ATPase 16 kDa proteolipid subunit

Gene

Vha16-1

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Proton-conducting pore forming subunit of the membrane integral V0 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei143Essential for proton translocationBy similarity1

GO - Molecular functioni

GO - Biological processi

  • ATP hydrolysis coupled proton transport Source: FlyBase
  • dsRNA transport Source: FlyBase
  • imaginal disc-derived wing morphogenesis Source: FlyBase
  • lysosomal lumen acidification Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-1222556. ROS, RNS production in response to bacteria.
R-DME-6798695. Neutrophil degranulation.
R-DME-77387. Insulin receptor recycling.
R-DME-917977. Transferrin endocytosis and recycling.
R-DME-983712. Ion channel transport.

Protein family/group databases

TCDBi9.B.16.1.1. the putative ductin channel (ductin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
V-type proton ATPase 16 kDa proteolipid subunit
Short name:
V-ATPase 16 kDa proteolipid subunit
Short name:
VHA16K
Alternative name(s):
Ductin
Vacuolar H+ ATPase subunit 16-1
Vacuolar proton pump 16 kDa proteolipid subunit
Gene namesi
Name:Vha16-1
Synonyms:VHA, Vha16
ORF Names:CG3161
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0262736. Vha16-1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12LumenalSequence analysisAdd BLAST12
Transmembranei13 – 35HelicalSequence analysisAdd BLAST23
Topological domaini36 – 57CytoplasmicSequence analysisAdd BLAST22
Transmembranei58 – 78HelicalSequence analysisAdd BLAST21
Topological domaini79 – 96LumenalSequence analysisAdd BLAST18
Transmembranei97 – 118HelicalSequence analysisAdd BLAST22
Topological domaini119 – 130CytoplasmicSequence analysisAdd BLAST12
Transmembranei131 – 156HelicalSequence analysisAdd BLAST26
Topological domaini157 – 159LumenalSequence analysis3

GO - Cellular componenti

  • fusome Source: FlyBase
  • integral component of membrane Source: GO_Central
  • plasma membrane proton-transporting V-type ATPase complex Source: FlyBase
  • vacuolar proton-transporting V-type ATPase, V0 domain Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000717501 – 159V-type proton ATPase 16 kDa proteolipid subunitAdd BLAST159

Proteomic databases

PaxDbiP23380.
PRIDEiP23380.

Expressioni

Gene expression databases

BgeeiFBgn0262736.
GenevisibleiP23380. DM.

Interactioni

Subunit structurei

V-ATPase is a heteromultimeric enzyme composed of a peripheral catalytic V1 complex (main components: subunits A, B, C, D, E, and F) attached to an integral membrane V0 proton pore complex (main component: the proteolipid protein; which is present as a hexamer that forms the proton-conducting pore).

Protein-protein interaction databases

BioGridi68967. 7 interactors.
DIPiDIP-19390N.
IntActiP23380. 150 interactors.
MINTiMINT-304429.
STRINGi7227.FBpp0085483.

Structurei

3D structure databases

ProteinModelPortaliP23380.
SMRiP23380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0232. Eukaryota.
COG0636. LUCA.
GeneTreeiENSGT00550000074873.
InParanoidiP23380.
KOiK02155.
OMAiTCAIVFT.
OrthoDBiEOG091G0U1Y.
PhylomeDBiP23380.

Family and domain databases

InterProiIPR002379. ATPase_proteolipid_c-like_dom.
IPR000245. ATPase_proteolipid_csu.
IPR011555. ATPase_proteolipid_su_C_euk.
[Graphical view]
PfamiPF00137. ATP-synt_C. 2 hits.
[Graphical view]
PRINTSiPR00122. VACATPASE.
SUPFAMiSSF81333. SSF81333. 2 hits.
TIGRFAMsiTIGR01100. V_ATP_synt_C. 1 hit.

Sequencei

Sequence statusi: Complete.

P23380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSEVSSDNP IYGPFFGVMG AASAIIFSAL GAAYGTAKSG TGIAAMSVMR
60 70 80 90 100
PELIMKSIIP VVMAGIIAIY GLVVAVLIAG ALEEPSKYSL YRGFIHLGAG
110 120 130 140 150
LAVGFSGLAA GFAIGIVGDA GVRGTAQQPR LFVGMILILI FAEVLGLYGL

IVAIYLYTK
Length:159
Mass (Da):16,267
Last modified:November 1, 1991 - v1
Checksum:i9F147F8D72C72123
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55979 mRNA. Translation: CAA39449.1.
X77936 Genomic DNA. Translation: CAA54908.1.
AE013599 Genomic DNA. Translation: AAF57359.1.
AE013599 Genomic DNA. Translation: AAF57360.1.
AE013599 Genomic DNA. Translation: AAM68381.1.
BT012440 mRNA. Translation: AAS93711.1.
PIRiS42878.
RefSeqiNP_476801.1. NM_057453.5.
NP_724474.1. NM_165474.3.
NP_724475.1. NM_165475.3.
NP_724476.1. NM_165476.3.
UniGeneiDm.6911.

Genome annotation databases

EnsemblMetazoaiFBtr0086150; FBpp0085483; FBgn0262736.
FBtr0086151; FBpp0085484; FBgn0262736.
FBtr0086152; FBpp0085485; FBgn0262736.
FBtr0086153; FBpp0085486; FBgn0262736.
GeneIDi44307.
KEGGidme:Dmel_CG3161.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X55979 mRNA. Translation: CAA39449.1.
X77936 Genomic DNA. Translation: CAA54908.1.
AE013599 Genomic DNA. Translation: AAF57359.1.
AE013599 Genomic DNA. Translation: AAF57360.1.
AE013599 Genomic DNA. Translation: AAM68381.1.
BT012440 mRNA. Translation: AAS93711.1.
PIRiS42878.
RefSeqiNP_476801.1. NM_057453.5.
NP_724474.1. NM_165474.3.
NP_724475.1. NM_165475.3.
NP_724476.1. NM_165476.3.
UniGeneiDm.6911.

3D structure databases

ProteinModelPortaliP23380.
SMRiP23380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi68967. 7 interactors.
DIPiDIP-19390N.
IntActiP23380. 150 interactors.
MINTiMINT-304429.
STRINGi7227.FBpp0085483.

Protein family/group databases

TCDBi9.B.16.1.1. the putative ductin channel (ductin) family.

Proteomic databases

PaxDbiP23380.
PRIDEiP23380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0086150; FBpp0085483; FBgn0262736.
FBtr0086151; FBpp0085484; FBgn0262736.
FBtr0086152; FBpp0085485; FBgn0262736.
FBtr0086153; FBpp0085486; FBgn0262736.
GeneIDi44307.
KEGGidme:Dmel_CG3161.

Organism-specific databases

CTDi44307.
FlyBaseiFBgn0262736. Vha16-1.

Phylogenomic databases

eggNOGiKOG0232. Eukaryota.
COG0636. LUCA.
GeneTreeiENSGT00550000074873.
InParanoidiP23380.
KOiK02155.
OMAiTCAIVFT.
OrthoDBiEOG091G0U1Y.
PhylomeDBiP23380.

Enzyme and pathway databases

ReactomeiR-DME-1222556. ROS, RNS production in response to bacteria.
R-DME-6798695. Neutrophil degranulation.
R-DME-77387. Insulin receptor recycling.
R-DME-917977. Transferrin endocytosis and recycling.
R-DME-983712. Ion channel transport.

Miscellaneous databases

GenomeRNAii44307.
PROiP23380.

Gene expression databases

BgeeiFBgn0262736.
GenevisibleiP23380. DM.

Family and domain databases

InterProiIPR002379. ATPase_proteolipid_c-like_dom.
IPR000245. ATPase_proteolipid_csu.
IPR011555. ATPase_proteolipid_su_C_euk.
[Graphical view]
PfamiPF00137. ATP-synt_C. 2 hits.
[Graphical view]
PRINTSiPR00122. VACATPASE.
SUPFAMiSSF81333. SSF81333. 2 hits.
TIGRFAMsiTIGR01100. V_ATP_synt_C. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVATL_DROME
AccessioniPrimary (citable) accession number: P23380
Secondary accession number(s): A4UZ55
, Q0E9N6, Q53XD4, Q9V9D2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: November 30, 2016
This is version 159 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.