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P23373

- E10_VACCW

UniProt

P23373 - E10_VACCW

Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

VACWR066

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves thiol-disulfide transfer between E10, A2.5 and G4.1 PublicationPROSITE-ProRule annotation

    Catalytic activityi

    2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

    Cofactori

    FAD.PROSITE-ProRule annotation

    GO - Molecular functioni

    1. thiol oxidase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
    Gene namesi
    Ordered Locus Names:VACWR066
    ORF Names:E10R
    OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
    Taxonomic identifieri10254 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiBos taurus (Bovine) [TaxID: 9913]
    ProteomesiUP000000344: Genome

    Subcellular locationi

    Virion membrane 1 Publication
    Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).Curated

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. viral envelope Source: UniProtKB-KW
    3. virion membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Membrane, Viral envelope protein, Virion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099465Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.1 Publication

    Keywords - Developmental stagei

    Late protein

    Interactioni

    Subunit structurei

    Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.1 Publication

    Structurei

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88IntravirionSequence Analysis
    Topological domaini26 – 9570Virion surfaceSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2517HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 9595ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the poxviridae E10 family.Curated
    Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Redox-active center, Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.20.120.310. 1 hit.
    InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view]
    PfamiPF04805. Pox_E10. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015696. VAC_E10R. 1 hit.
    SUPFAMiSSF69000. SSF69000. 1 hit.
    PROSITEiPS51324. ERV_ALR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P23373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA   50
    TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVT KVNPL 95
    Length:95
    Mass (Da):10,851
    Last modified:November 1, 1991 - v1
    Checksum:iF4A2FAB4159DFA50
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36339 Genomic DNA. Translation: AAB59830.1.
    AY243312 Genomic DNA. Translation: AAO89345.1.
    PIRiT30803.
    RefSeqiYP_232948.1. NC_006998.1.

    Genome annotation databases

    GeneIDi3707599.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M36339 Genomic DNA. Translation: AAB59830.1 .
    AY243312 Genomic DNA. Translation: AAO89345.1 .
    PIRi T30803.
    RefSeqi YP_232948.1. NC_006998.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 3707599.

    Family and domain databases

    Gene3Di 1.20.120.310. 1 hit.
    InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
    IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
    [Graphical view ]
    Pfami PF04805. Pox_E10. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015696. VAC_E10R. 1 hit.
    SUPFAMi SSF69000. SSF69000. 1 hit.
    PROSITEi PS51324. ERV_ALR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of rpo30, a vaccinia virus RNA polymerase gene with structural similarity to a eucaryotic transcription elongation factor."
      Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.
      Mol. Cell. Biol. 10:5433-5441(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
      Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Vaccinia virus E10R protein is associated with the membranes of intracellular mature virions and has a role in morphogenesis."
      Senkevich T.G., Weisberg A.S., Moss B.
      Virology 278:244-252(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, INDUCTION.
    4. "Complete pathway for protein disulfide bond formation encoded by poxviruses."
      Senkevich T.G., White C.L., Koonin E.V., Moss B.
      Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH A2.5.

    Entry informationi

    Entry nameiE10_VACCW
    AccessioniPrimary (citable) accession number: P23373
    Secondary accession number(s): Q76ZV5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1991
    Last sequence update: November 1, 1991
    Last modified: October 1, 2014
    This is version 62 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3