P23373 (E10R_VACCW) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable FAD-linked sulfhydryl oxidase E10
EC=1.8.3.2

Gene names

Ordered Locus Names:	VACWR066
ORF Names:	E10R

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves thiol-disulfide transfer between E10, A2.5 and G4. Ref.4
Catalytic activity	2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.
Cofactor	FAD By similarity.
Subunit structure	Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer. Ref.4
Subcellular location	Virion membrane Probable. Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV) Probable. Ref.3
Induction	Expressed in the late phase of the viral replicative cycle. Ref.3
Sequence similarities	Belongs to the poxviridae E10 family. Contains 1 ERV/ALR sulfhydryl oxidase domain.

Ontologies

Keywords
Cellular component	Membrane Viral envelope protein Virion
Developmental stage	Late protein
Domain	Redox-active center Transmembrane Transmembrane helix
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiol oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Probable FAD-linked sulfhydryl oxidase E10		PRO_0000099465
Regions
	Transmembrane	9 – 25	17	Helical; Potential
	Domain	1 – 95	95	ERV/ALR sulfhydryl oxidase
Amino acid modifications
	Disulfide bond	43 ↔ 46		Redox-active Potential

Sequences

	Sequence		Length	Mass (Da)
	P23373 [UniParc]. Last modified November 1, 1991. Version 1. Checksum: F4A2FAB4159DFA50	FASTA	95	10,851
	10 20 30 40 50 60 MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA TIAIEDNNVM 70 80 90 SSDDLNYIYY FFIRLFNNLA SDPKYAIDVT KVNPL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of rpo30, a vaccinia virus RNA polymerase gene with structural similarity to a eucaryotic transcription elongation factor." Ahn B.-Y., Gershon P.D., Jones E.V., Moss B. Mol. Cell. Biol. 10:5433-5441(1990) [PubMed: 2398897] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"Vaccinia virus E10R protein is associated with the membranes of intracellular mature virions and has a role in morphogenesis." Senkevich T.G., Weisberg A.S., Moss B. Virology 278:244-252(2000) [PubMed: 11112499] [Abstract] Cited for: SUBCELLULAR LOCATION, INDUCTION.
[4]	"Complete pathway for protein disulfide bond formation encoded by poxviruses." Senkevich T.G., White C.L., Koonin E.V., Moss B. Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002) [PubMed: 11983854] [Abstract] Cited for: FUNCTION, INTERACTION WITH A2.5.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M36339 Genomic DNA. Translation: AAB59830.1. AY243312 Genomic DNA. Translation: AAO89345.1.
PIR	T30803.
RefSeq	YP_232948.1. NC_006998.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3707599.
Phylogenomic databases
ProtClustDB	PHA3005.
Family and domain databases
InterPro	IPR017905. ERV/ALR_sulphydryl_oxidase. IPR006863. Evr1_Alr. IPR006890. Sulphydryl_Oase_FAD-link_ERV1. [Graphical view]
Gene3D	G3DSA:1.20.120.310. Evr1_Alr. 1 hit.
Pfam	PF04805. Pox_E10. 1 hit. [Graphical view]
PIRSF	PIRSF015696. VAC_E10R. 1 hit.
PROSITE	PS51324. ERV_ALR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E10R_VACCW

Accession

Primary (citable) accession number: P23373
Secondary accession number(s): Q76ZV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1991
Last sequence update:	November 1, 1991
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program