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P23373

- E10_VACCW

UniProt

P23373 - E10_VACCW

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Protein

Probable FAD-linked sulfhydryl oxidase E10

Gene

VACWR066

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

FAD-dependent sulfhydryl oxidase that catalyzes disulfide bond formation. The complete pathway for formation of disulfide bonds in intracellular virion membrane proteins sequentially involves thiol-disulfide transfer between E10, A2.5 and G4.1 PublicationPROSITE-ProRule annotation

Catalytic activityi

2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2.

Cofactori

FAD.PROSITE-ProRule annotation

GO - Molecular functioni

  1. thiol oxidase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

FAD, Flavoprotein

Names & Taxonomyi

Protein namesi
Recommended name:
Probable FAD-linked sulfhydryl oxidase E10 (EC:1.8.3.2)
Gene namesi
Ordered Locus Names:VACWR066
ORF Names:E10R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
ProteomesiUP000000344: Genome

Subcellular locationi

Virion membrane 1 Publication
Note: Associated with crescent membranes, immature virions (IV) and mature virions (MV).Curated

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. viral envelope Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 9595Probable FAD-linked sulfhydryl oxidase E10PRO_0000099465Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi43 ↔ 46Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Expressioni

Inductioni

Expressed in the late phase of the viral replicative cycle.1 Publication

Keywords - Developmental stagei

Late protein

Interactioni

Subunit structurei

Interacts with A2.5; this interaction involves formation of a transient disulfide-bonded intermediate, allowing disulfide bond transfer.1 Publication

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88IntravirionSequence Analysis
Topological domaini26 – 9570Virion surfaceSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2517HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 9595ERV/ALR sulfhydryl oxidasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the poxviridae E10 family.Curated
Contains 1 ERV/ALR sulfhydryl oxidase domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.20.120.310. 1 hit.
InterProiIPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view]
PfamiPF04805. Pox_E10. 1 hit.
[Graphical view]
PIRSFiPIRSF015696. VAC_E10R. 1 hit.
SUPFAMiSSF69000. SSF69000. 1 hit.
PROSITEiPS51324. ERV_ALR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P23373-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNPKHWGRAV WTIIFIVLSQ AGLDGNIEAC KRKLYTIVST LPCPACRRHA
60 70 80 90
TIAIEDNNVM SSDDLNYIYY FFIRLFNNLA SDPKYAIDVT KVNPL
Length:95
Mass (Da):10,851
Last modified:November 1, 1991 - v1
Checksum:iF4A2FAB4159DFA50
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36339 Genomic DNA. Translation: AAB59830.1.
AY243312 Genomic DNA. Translation: AAO89345.1.
PIRiT30803.
RefSeqiYP_232948.1. NC_006998.1.

Genome annotation databases

GeneIDi3707599.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M36339 Genomic DNA. Translation: AAB59830.1 .
AY243312 Genomic DNA. Translation: AAO89345.1 .
PIRi T30803.
RefSeqi YP_232948.1. NC_006998.1.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 3707599.

Family and domain databases

Gene3Di 1.20.120.310. 1 hit.
InterProi IPR017905. ERV/ALR_sulphydryl_oxidase.
IPR006890. Sulphydryl_Oase_FAD-link_ERV1.
[Graphical view ]
Pfami PF04805. Pox_E10. 1 hit.
[Graphical view ]
PIRSFi PIRSF015696. VAC_E10R. 1 hit.
SUPFAMi SSF69000. SSF69000. 1 hit.
PROSITEi PS51324. ERV_ALR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of rpo30, a vaccinia virus RNA polymerase gene with structural similarity to a eucaryotic transcription elongation factor."
    Ahn B.-Y., Gershon P.D., Jones E.V., Moss B.
    Mol. Cell. Biol. 10:5433-5441(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb."
    Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R.
    Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Vaccinia virus E10R protein is associated with the membranes of intracellular mature virions and has a role in morphogenesis."
    Senkevich T.G., Weisberg A.S., Moss B.
    Virology 278:244-252(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INDUCTION.
  4. "Complete pathway for protein disulfide bond formation encoded by poxviruses."
    Senkevich T.G., White C.L., Koonin E.V., Moss B.
    Proc. Natl. Acad. Sci. U.S.A. 99:6667-6672(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH A2.5.

Entry informationi

Entry nameiE10_VACCW
AccessioniPrimary (citable) accession number: P23373
Secondary accession number(s): Q76ZV5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1991
Last sequence update: November 1, 1991
Last modified: October 29, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3